Lumican is overexpressed in lung adenocarcinoma pleural effusions.

Adenocarcinoma (AdC) is the most common lung cancer subtype and is often associated with pleural effusion (PE). Its poor prognosis is attributable to diagnostic delay and lack of effective treatments and there is a pressing need in discovering new biomarkers for early diagnosis or targeted therapies. To date, little is known about lung AdC proteome. We investigated protein expression of lung AdC in PE using the isobaric Tags for Relative and Absolute Quantification (iTRAQ) approach to identify possible novel diagnostic/prognostic biomarkers. This provided the identification of 109 of lung AdC-related proteins. We further analyzed lumican, one of the overexpressed proteins, in 88 resected lung AdCs and in 23 malignant PE cell-blocks (13 lung AdCs and 10 non-lung cancers) using immunohistochemistry. In AdC surgical samples, lumican expression was low in cancer cells, whereas it was strong and diffuse in the stroma surrounding the tumor. However, lumican expression was not associated with tumor grade, stage, and vascular/pleural invasion. None of the lung cancer cell-blocks showed lumican immunoreaction, whereas those of all the other tumors were strongly positive. Finally, immunoblotting analysis showed lumican expression in both cell lysate and conditioned medium of a fibroblast culture but not in those of A549 lung cancer cell line. PE is a valid source of information for proteomic analysis without many of the restrictions of plasma. The high lumican levels characterizing AdC PEs are probably due to its release by the fibroblasts surrounding the tumor. Despite the role of lumican in lung AdC is still elusive, it could be of diagnostic value

Redes personales y trayectorias educativas de mujeres con estudios universitarios con el Plan social Jefas y Jefes de Hogar

Se abordan trayectorias vitales y educativas, redes personales, obstáculos y logros de tres mujeres que, siendo titulares de un plan social, obtuvieron un título universitario en una carrera de corte social. Investigación con un método mixto, con predominio cualitativo, con un diseño de anidado o incrustado concurrente. Objetivos: conocer las trayectorias vitales y educativas de estas mujeres y describir sus redes personales, identificando características y tipo de apoyo recibido. Los instrumentos utilizados para recabar la información fueron una entrevista semiestructurada ad hoc y un análisis de las redes personales. En el análisis de las redes sociales, se utilizó el software Egonet y el análisis cualitativo para las entrevistas. Sus trayectorias vitales permiten vislumbrar una acumulación de desventajas o exclusiones que, debido a ciertas características personales (perseverancia y expectativas de logro), de sus redes (unión y cooperación grupal) y la oportunidad, como beneficiarias de un plan social laboral, de materializar su contraprestación en estudios de nivel superior, obtuvieron un título de licenciatura

LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate

Background Lrrk2, a gene linked to Parkinson\u2019s disease, encodes a large scaffolding protein with kinase and GTPase activities implicated in vesicle and cytoskeletal-related processes. At the presynaptic site, LRRK2 associates with synaptic vesicles through interaction with a panel of presynaptic proteins. Results Here, we show that LRRK2 kinase activity influences the dynamics of synaptic vesicle fusion. We therefore investigated whether LRRK2 phosphorylates component(s) of the exo/endocytosis machinery. We have previously observed that LRRK2 interacts with NSF, a hexameric AAA+ ATPase that couples ATP hydrolysis to the disassembling of SNARE proteins allowing them to enter another fusion cycle during synaptic exocytosis. Here, we demonstrate that NSF is a substrate of LRRK2 kinase activity. LRRK2 phosphorylates full-length NSF at threonine 645 in the ATP binding pocket of D2 domain. Functionally, NSF phosphorylated by LRRK2 displays enhanced ATPase activity and increased rate of SNARE complex disassembling. Substitution of threonine 645 with alanine abrogates LRRK2-mediated increased ATPase activity. Conclusions Given that the most common Parkinson\u2019s disease LRRK2 G2019S mutation displays increased kinase activity, our results suggest that mutant LRRK2 may impair synaptic vesicle dynamics via aberrant phosphorylation of NSF

Redes pessoais e trajetórias educativas de mulheres com estudos universitários com o plano social chefas e chefes de família

Se abordan trayectorias vitales y educativas, redes personales, obstáculos y logros de tres mujeres que, siendo titulares de un plan social, obtuvieron un título universitario en una carrera de corte social. Investigación con un método mixto, con predominio cualitativo, con un diseño de anidado o incrustado concurrente. Objetivos: conocer las trayectorias vitales y educativas de estas mujeres y describir sus redes personales, identificando características y tipo de apoyo recibido. Los instrumentos utilizados para recabar la información fueron una entrevista semiestructurada ad hoc y un análisis de las redes personales. En el análisis de las redes sociales, se utilizó el software Egonet y el análisis cualitativo para las entrevistas. Sus trayectorias vitales permiten vislumbrar una acumulación de desventajas o exclusiones que, debido a ciertas características personales (perseverancia y expectativas de logro), de sus redes (unión y cooperación grupal) y la oportunidad, como beneficiarias de un plan social laboral, de materializar su contraprestación en estudios de nivel superior, obtuvieron un título de licenciatura.The present study addresses the life and educational trajectories, personal network, obstacles and achievements of three women who, as holders of a social plan, obtained a bachelor's degree in a universitylevel career. This is a mixed method research, with qualitative predominance, with a concurrent nested or embedded design. Objectives: to know life and educational trajectories of these women and to describe their personal networks, identifying their characteristics and the type of support received from them. The instruments used to collect the information were an ad hoc semi-structured interview and personal networks analysis. Egonet software and qualitative analysis were used in the social media analysis for the interviews. Participants' life trajectories allow us to glimpse an accumulation of disadvantages or exclusions that, due to certain personal characteristics (perseverance and expectations of achievement), to their personal networks (union and group cooperation) and to the opportunity provided as beneficiaries of a social labor plan to access to a higher level education, they were able to complete their studies.Analisam-se trajetórias vitais e educativas, redes pessoais, obstáculos e logros de três mulheres que, sendo titulares de um plano social, obtiveram um título universitário em uma carreira de índole social. Investigação com um método misto, com predomínio qualitativo, com um desenho aninhado dentro de um método dominante. Objetivos: conhecer as trajetórias vitais e educativas destas mulheres e descrever suas redes pessoais, identificando características e tipo de apoio recebido. Os instrumentos utilizados para coletar a informação foram entrevistas semiestruturadas ad hoc e uma análise das redes pessoais. Na análise das redes sociais utilizou-se o software Egonet e a análise qualitativa para as entrevistas. Suas trajetórias vitais permitem vislumbrar uma acumulação de desvantagens ou exclusões que, devido a determinadas características pessoais (perseverança e expectativas de logro), de suas redes (a união e a cooperação grupal) e a oportunidade, como beneficiárias de um plano social de trabalho de materializar sua contraprestação em estudos de nível superior, obtiveram um título de licenciatura.Fil: Arrigoni, Flavia Susana. Universidad del Aconcagua. Facultad de Psicología; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Paredes, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; Argentina. Universidad del Aconcagua. Facultad de Psicología; ArgentinaFil: Muñoz Rodríguez, Laura Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Departamento de Psicología. Laboratorio de Investigación en Ciencias del Comportamiento; Argentin

Personal networks and resilience: A study of female inmates in a prisons of Argentina

El presente estudio aborda las características de las redes personales y la resiliencia de doce mujeres penadas privadas de libertad alojadas en una unidad penal de mujeres en Mendoza. Se trata de una investigación con alcance descriptivo y diseño no experimental, transeccional, de enfoque cuantitativo. El objetivo central es describir las características de la resiliencia y de las redes personales que posee un grupo de mujeres penadas, privadas de libertad. Los instrumentos utilizados para recabar la información fueron: entrevista estructurada para evaluar factores de riesgo, de protección y de su potencial resiliente en contexto carcelario- ERAC (Mikulik y Crespi, 2004). Igualmente, con el software Egonet se analizaron sus redes personales como fuente de apoyo. Se evidencia el impacto del encierro en las redes personales y se hacen necesarios los beneficios de programas basados en la promoción de los factoresprotectores. Esto requiere, por un lado, proponer estrategias de intervención interdisciplinarias e integrales para el desarrollo de la resiliencia, con el fin de asegurar una futura reinserción social más exitosa. Por otro lado, resulta necesario diseñar e implementar programas que promuevan el desarrollo de aspectos salutogénicos, que incrementen el bienestar de las mujeres durante su privación de libertad.This study addresses the characteristics of personal networks and resilience of twelve female inmates who have been found guilty and are in the “Unidad Penal de Mujeres” in Mendoza. It´s a descriptive study, with a non-experimental, cross-sectional design; with a quantitative approach. The main aim is to analyze the relationship between the characteristics of social networks and resilience among a group of female inmates. The instruments used to gather information were: structured interview to evaluate risk and protective factors and resilience potential in a prison contextERAC (Mikulik & Crespi, 2005) and personal networks analysis considering them as a support group with the software Egonet. It was observed that their relationships with others are the most affected factors of resilience. Therefore, the impact of the confinement in the personal networks becomes apparent as well as the need of those benefits offered by programs based in the promotion of protective factors. This requires, on the one hand, to propose interdisciplinary and integrative intervention strategies to develop resilience in order to assure a more successful future social insertion; and, on the other hand, to design and to implement programs that promote the development of salutogenic aspects that increase the wellbeing of women during their imprisonment.Fil: Paredes, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Muñoz Rodríguez, Laura Mariela. Universidad Nacional de San Luis. Facultad de Ciencias Humanas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arrigoni, Flavia Susana. Universidad del Aconcagua. Facultad de Psicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

¿Pan para hoy, hambre para mañana?: Una iniciativa de extensión para el desarrollo sustentable

Nuestro trabajo pretende reflexionar sobre el concepto de extensión, buscando un enfoque que pueda guiar nuestra práctica en el ámbito agronómico, anhelando que pueda resultar valiosa, transformadora y constructiva. Partiremos de una aproximación general, derivando en una experiencia particular de extensión: el proyecto “Pan para hoy, hambre para mañana”, radicado en la Facultad de Agronomía de Azul durante el período 2016-2017. El objetivo de este último fue generar un espacio de extensión donde participen jóvenes de escuelas secundarias agrarias, para que puedan compartir los conocimientos aprendidos y las técnicas para ponerlos en práctica en su entorno cercano. Esta intervención permitiría mejorar el sistema agrícola triguero tendiendo a cubrir necesidades particulares de los pequeños productores de la zona, como son la competitividad, la sustentabilidad y la subsistencia. En cuanto al impacto del proyecto, se percibió un notable interés por parte de los estudiantes en las temáticas planteadas, demostrando la importancia de la articulación interinstitucional para ampliar los campos de acción y transformación abordados por el sistema educativo. Otro aspecto importante fue el debate y la autoevaluación que se propició dentro del grupo de trabajo en la Universidad Nacional del Centro de la Provincia de Buenos Aires. Por lo tanto, consideramos que este proyecto constituye un primer paso en el vínculo entre ambos niveles educativos con relación a la temática planteada, surgiendo un abanico de posibilidades para su continuidad ampliando los sujetos participantes y las problemáticas abordadas. Finalmente, consideramos que una Educación Agraria inclusiva, accesible y de calidad en todos sus niveles constituye un pilar fundamental en la sustentabilidad de la producción agropecuaria, para que “pan para hoy” no signifique “hambre para mañana”.Fil: Arata, Agustin Francisco. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Arrigoni, Adriana Cecilia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Lázaro, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Pérez, Daniel Rubén. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Videla, Yanina Paola. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Centro Regional de Estudios Sistémico de Cadenas Agroalimentarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentin

Metal line emission from galaxy haloes at z~1

We present a study of the metal-enriched halo gas, traced using MgII and [OII] emission lines, in two large, blind galaxy surveys - the MUSE (Multi Unit Spectroscopic Explorer) Analysis of Gas around Galaxies (MAGG) and the MUSE Ultra Deep Field (MUDF). By stacking a sample of ~600 galaxies (stellar masses M* ~10^{6-12} Msun), we characterize for the first time the average metal line emission from a general population of galaxy haloes at 0.7 <= z <= 1.5. The MgII and [OII] line emission extends farther out than the stellar continuum emission, on average out to ~25 kpc and ~45 kpc, respectively, at a surface brightness (SB) level of 10^{-20} erg/s/cm2/arcsec2. The radial profile of the MgII SB is shallower than that of the [OII], suggesting that the resonant MgII emission is affected by dust and radiative transfer effects. The [OII] to MgII SB ratio is ~3 over ~20-40 kpc, also indicating a significant in situ origin of the extended metal emission. The average SB profiles are intrinsically brighter by a factor ~2-3 and more radially extended by a factor of ~1.3 at 1.0 < z <= 1.5 than at 0.7 <= z <= 1.0. The average extent of the metal emission also increases independently with increasing stellar mass and in overdense group environments. When considering individual detections, we find extended [OII] emission up to ~50 kpc around ~30-40 percent of the group galaxies, and extended (~30-40 kpc) MgII emission around two z~1 quasars in groups, which could arise from outflows or environmental processes.Comment: 24 pages, 21 figures, 2 tables, accepted for publication in MNRA

Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue

Previous work indicated that lysine-specific demethylase 1 (Lsd1) can positively regulate the oxidative and thermogenic capacities of white and beige adipocytes. Here we investigate the role of Lsd1 in brown adipose tissue (BAT) and find that BAT- selective Lsd1 ablation induces a shift from oxidative to glycolytic metabolism. This shift is associated with downregulation of BAT-specific and upregulation of white adipose tissue (WAT)-selective gene expression. This results in the accumulation of di- and triacylglycerides and culminates in a profound whitening of BAT in aged Lsd1- deficient mice. Further studies show that Lsd1 maintains BAT properties via a dual role. It activates BAT-selective gene expression in concert with the transcription factor Nrf1 and represses WAT-selective genes through recruitment of the CoREST complex. In conclusion, our data uncover Lsd1 as a key regulator of gene expression and metabolic function in BAT

Stripped gas as fuel for newly formed HII regions in the encounter between VCC1249 and M49: a unified picture from NGVS and GUViCS

Context: We study the peculiar interacting galaxy system of VCC1249/M49 located in the core of the Virgo B subcluster. Owing to a recent interaction between the dwarf galaxy VCC1249 and the halo gas of the gE M49, neutral hydrogen has been displaced from the interstellar medium of this dwarf into the Virgo ICM. Observations also reveal multiple compact star-forming regions that are embedded in this HI cloud, with a projected separation up to 13 kpc from VCC1249 in the northwest direction. Aims: Motivated by recent NUV imaging from GUViCS of the VCC1249/M49 system that shows significant ongoing/recent star formation in the compact regions, we aim to constrain the origin of these outlying HII regions with a multi-wavelength approach. Methods: Using deep optical (u, g, i, z) imaging from NGVS and new Halpha imaging obtained at the San Pedro Martir observatory together with Keck long-slit spectroscopy, we characterize the SFR, ages, and metallicity of VCC1249 and its outlying compact regions. Moreover, we analyze the color and luminosity profile of the galaxy to investigate its recent interaction with M49. Results: Our new observations indicate that VCC1249 underwent a recent interaction with M49 in which both ram-pressure stripping and tidal interaction occured. The joint action of the two mechanisms led to the removal of the HI gas from the ISM of VCC1249, while the gravitational tides triggered the stellar tail and counter-tail of VCC1249. Our SED analysis reveals that the star formation in this galaxy was truncated around 200 Myr ago and that the outlying HII regions were born in situ about 10 Myr ago out of pre-enriched gas removed from the dwarf galaxy. These observations also reveal that interactions between central and satellite galaxies similar to the one between VCC1249/M49 may be an effective way of dispersing metals into the halos of massive galaxies.Comment: 19 pages, 17 figures; one co-author adde

Cytosolic sequestration of spatacsin by Protein Kinase A and 14-3-3 proteins

Mutations in SPG11, encoding spatacsin, constitute the major cause of autosomal recessive Hereditary Spastic Paraplegia (HSP) with thinning of the corpus callosum. Previous studies showed that spatacsin orchestrates cellular traffic events through the formation of a coat-like complex and its loss of function results in lysosomal and axonal transport impairments. However, the upstream mechanisms that regulate spatacsin trafficking are unknown. Here, using proteomics and CRISPR/Cas9-mediated tagging of endogenous spatacsin, we identified a subset of 14-3-3 proteins as physiological interactors of spatacsin. The interaction is modulated by Protein Kinase A (PKA)-dependent phosphorylation of spatacsin at Ser1955, which initiates spatacsin trafficking from the plasma membrane to the intracellular space. Our study provides novel insight in understanding spatacsin physio- pathological roles with mechanistic dissection of its associated pathways