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Restoration and preservation of the anatomical specimens of the Museum of Pathological Anatomy at the University of Pisa

The recent establishment of the University Museum System, whose aim is to promote and enhance the University collections, encouraged the rearrangement of the Museum of Pathological Anatomy, currently not operating, because its precious pieces are stored in a warehouse. The economic support of the “Fondazione Pisa” permitted, in the last year, to begin the recovery and restoration of a part of the specimens. The collection consists in 1500 human and animal pathological specimens of great scientific relevance, some of which dated back to the Granducal period. The remains consist in pathological changes and congenital anomalies detected on human and animal bodies and organs, preserved in formaldehyde or dry. In particular, the Museum includes: a collection of 50 human bladder stones dating back to the first half of the 19th century; a collection of malformed human newborns documenting 25 rare congenital malformations, dating back to the end of 19th and the beginning of 20th century; a collection of animal teratology; a collection of helminthic parasitology. In the last 30 years, the Museum was then enriched with a collection of pieces from pathological autopsies, such as lung, cardio-vascular, renal, and brain diseases. The recovery was initially addressed to restore the wet preparations preserved in formaldehyde that required an urgent emergency. In fact, in many cases the evaporation of the liquid has determined the deterioration of the specimens; moreover, formalin was replaced with alcohol because it has been declared toxic and the new Museum dispositions require impose the substitution with not dangerous preserving liquids. In the GiPaleo Meeting a selection from over 100 artifacts restored will be exhibited

Wild cat and domestic cat: domestication or taming ?

This study point out the relationship between wild cat and domestic cat correlating the craniometric data of wild cats from literature to those from bone remains of the Museum of Veterinary Anatomy (University of Pisa). The morphological and morphometrical features closely associated with behavioral attitudes confirm the hypothesis that the cat would be considered as a taming animal

Growth in glucose-based medium and exposure to subinhibitory concentrations of imipenem induce biofilm formation in a multidrug-resistant clinical isolate of Acinetobacter baumannii

<p>Abstract</p> <p>Background</p> <p><it>Acinetobacter baumannii </it>is emerging as an important nosocomial pathogen. Multidrug resistance, as well as ability to withstand environmental stresses, makes eradication of <it>A. baumannii </it>difficult, particularly from hospital settings.</p> <p>Results</p> <p>Over a six-year period, 73 isolates of <it>A. baumannii </it>were collected from infected patients in two hospitals in Italy. While 69 out of the 73 isolates displayed identical multidrug antibiotic resistance pattern, they were susceptible to carbapenems. Genetic profiles of these 69 isolates, determined by Pulsed Field Gel Electrophoresis (PFGE), indicated that they were genetically related and could be clustered in a specific clone, called SMAL. We tested the ability of the SMAL clone to form biofilm, an important determinant for bacterial colonization of the human host and for persistence in the hospital environment. Biofilm formation by <it>A. baumannii </it>SMAL, measured as surface adhesion to polystyrene, is strongly affected by growth conditions, being impaired in rich growth media such as LB, while being favoured in glucose-based medium. Surface adhesion in glucose-based media is inhibited by treatment with cellulase, suggesting that it depends on production of cellulose or of a chemically related extracellular polysaccharide. Exposure of <it>A. baumannii </it>SMAL to subinhibitory concentrations of imipenem resulted in biofilm stimulation and increased production of iron uptake proteins. Growth in iron-supplemented medium also stimulated surface adhesion, thus suggesting that increased intracellular iron concentrations might act as an environmental signal for biofilm formation in <it>A. baumannii </it>SMAL.</p> <p>Conclusions</p> <p>Our results indicate that exposure to subinhibitory concentrations of imipenem can stimulate biofilm formation and induce iron uptake in a pathogenic strain of <it>A. baumannii</it>, with potential implications on antibiotic susceptibility and ability to persist in the human host.</p

Exposición natural y envejecimiento acelerado de polietilenos para invernaderos, en diferentes condiciones climáticas

p.221-228Se evaluaron las propiedades mecánicas y ópticas de cuatro films de polietileno durante tres años de envejecimiento natural a la intemperie y mil horas de envejecimiento acelerado en laboratorio. Se expusieron en Yavi (Jujuy, Argentina) y se siguieron las normas A.S.T.M. Se correlacionaron los resultados obtenidos a la intemperie con los de envejecimiento acelerado para predecir la vida útil de un plástico, en condiciones climáticas similares, realizando sólo ensayos de laboratorio. Se observó que las propiedades mecánicas alcanzan más rápido las condiciones de envejecimiento y, por lo tanto, son mas relevantes para caracterizar el comportamiento de los mismos. Se analizaron las propiedades en función de la radiación solar y la precipitación acumulada, para independizarse de las variaciones climáticas. En Yavi la duración de los plásticos fue mayor que en Buenos Aires (Capital Federal, Argentina), en el mismo período. Se concluye que aunque la radiación en Yavi es elevada, el menor deterioro de los films probablemente sea por la baja precipitación y ausencia de contaminación

Conocer para elegir: una experiencia de articulación de la FCNYM con la Escuela Media

El taller “Conocer para elegir: disciplinas, incumbencias y perfil profesional del Graduado en Ciencias Naturales” es un taller integrado de articulación con la Escuela Media, surgido en el marco del Trayecto de Ingreso y Seguimiento de la Facultad de Ciencias Naturales y Museo de la Universidad Nacional de La Plata (FCNyM, UNLP), comprendido en el Plan de Acciones Complementarias Becas Bicentenario (Secretaría de Políticas Universitarias, Ministerio de Educación de la Nación). Se realizó en tres Instituciones educativas con el objetivo de promocionar las carreras de la FCNyM y dar a conocer algunos aspectos de la vida universitaria. El taller es dictado por un equipo interdisciplinario de graduados de Geología, Biología y Antropología de la FCNyM y está destinado a alumnos del último año de la escuela secundaria.Facultad de Ciencias Naturales y Muse

analysis of non animal methods and models for research in cardiovascular disease

Cardiovascular diseases (CVD) are disorders of the heart and blood vessels and represent 31% of all global deaths. In the contest of CVD, the use of animal experiments has been a contentious subject for many years. In recent years, in vitro and in silico models and methods have been proposed according to the 3Rs statement. However, an exhaustive report regarding the state of art in terms of in vitro and in silico experiments has not been reported yet. This work is focused on providing a collection of non-animal models and methods in use for basic and applied CVD research. The standardized descriptions of such studies will ultimately feed into EURL ECVAM database on alternative methods. Two are the research main phases. Firstly, the exclusion/ inclusion criteria and the list of relevant information resources of the research have been defined. The second phase regards the search, selection and detailed description of the literature papers by analysing records on Scopus and Pubmed databases

Gastrointestinal presentation of kawasaki disease: A red flag for severe disease?

Background Kawasaki disease (KD) is a febrile systemic vasculitis of unknown etiology and the main cause of acquired heart disease among children in the developed world. To date, abdominal involvement at presentation is not recognized as a risk factor for a more severe form of the disease. Objective To evaluate whether presenting abdominal manifestations identify a group at major risk for Intravenous immunoglobulin (IVIG)-resistance and coronary lesions. Methods Retrospective study of KD patients diagnosed between 2000 and 2015 in 13 pediatric units in Italy. Patients were divided into 2 groups according to the presence or absence of abdominal manifestations at onset. We compared their demographic and clinical data, IVIG-responsiveness, coronary ectasia/aneurysms, laboratory findings from the acute and subacute phases. Results 302 patients (181 boys) were enrolled: 106 patients with, and 196 patients without presenting abdominal features. Seasonality was different between the groups (p = 0.034). Patients with abdominal manifestations were younger (p = 0.006) and more frequently underwent delayed treatment (p = 0.014). In the acute phase, patients with abdominal presentation had higher platelet counts (PLT) (p = 0.042) and lower albuminemia (p = 0.009), while, in the subacute phase, they had higher white blood cell counts (WBC) and PLT (p = 0.002 and p < 0.005, respectively) and lower red blood cell counts (RBC) and hemoglobin (Hb) (p = 0.031 and p 0.009). Moreover, the above mentioned group was more likely to be IVIG-resistant (p < 0.005) and have coronary aneurysms (p = 0.007). In the multivariate analysis, presenting abdominal manifestations, age younger than 6 months, IVIG- resistance, delayed treatment and albumin concentration in the acute phase were independent risk factors for coronary aneurysms (respectively p<0.005, <0.005, = 0.005 and 0.009). Conclusions This is the first multicenter report demonstrating that presenting gastrointestinal features in KD identify patients at higher risk for IVIG-resistance and for the development of coronary aneurysms in a predominantly Caucasian population