Study and validation of data recorded in the vehicles’ EDR in order to perform a road accident’s dynamic reconstruction

Road accident reconstruction is an issue which involves multiple and differentiated subjects. A collision contours’ determination requires the investigation and the analysis of all the evidence provided from highly distinct sources and remaining from uncertain and, sometimes, chaotic scenarios. People are vastly involved in traffic accident situations, either being drivers, victims, injured or witnesses. Therefore, accident investigation is a sensitive matter which requires objectiveness, accuracy, efficiency, and effectiveness, to draw faithful and factual conclusions about the collisions’ contours. The accidents reconstruction science’s main objective is to determine and describe the involved vehicles dynamics, which is accomplished by collecting and interconnect all the available evidence extracted from the impacts’ scenarios, from the vehicles, and from the involved people. In the past, many authors developed mathematical models which describe, approximately, the vehicles’ dynamics involved in a road traffic collision. Over the years, with the technology evolution and the advances on the area, multiple solutions have been created and enhanced to provide to accident reconstructionists better and more reliable evidence, allowing them to perform crash reconstructions with higher accuracy. These solutions include numerical methods, simulation and evaluation software, and tools for evidence collection. However, the introduction of the Event Data Recorder (EDR) on the vehicles consists of a great progression concerning the availability of valid and meaningful clues which can be used as inputs for the scientific crash reconstruction, since the EDR stores data that was unavailable and was difficult to deduce from the accident’s remaining evidence, previously. On the scope of this project, a vehicle data logging device was developed and tested regarding the validation of the EDR’s recorded data. The device’s purpose is to acquire the most relevant variables for crash reconstruction, which are also stored by the EDR, and provide a source of information for comparison and validation. This device was integrated with the respective sensors, programmed with a developed software, and tested on a vehicle. The tests for dynamic data acquisition consisted of travelling a defined path around the school campus, since there was not the opportunity to perform a real crash test with an EDR equipped vehicle

Solving a case of allelic dropout in the GNPTAB gene: implications in the molecular diagnosis of mucolipidosis type III alpha/beta

While being well known that the diagnosis of many genetic disorders relies on a combination of clinical suspicion and confirmatory genetic testing, not rarely, however, genetic testing needs much perseverance and cunning strategies to identify the causative mutation(s). Here we present a case of a thorny molecular diagnosis of mucolipidosis type III alpha/beta, which is an autosomal recessive lysosomal storage disorder, caused by a defect in the GNPTAB gene that codes for the α/β-subunits of the GlcNAc-1-phosphotransferase. We used both cDNA and gDNA analyses to characterize a mucolipidosis type III alpha/beta patient whose clinical diagnosis was already confirmed biochemically. In a first stage only one causal mutation was identified in heterozygosity, the already described missense mutation c.1196C>T(p.S399F), both at cDNA and gDNA levels. Only after conducting inhibition of nonsense-mediated mRNA decay (NMD) assays and after the utilization of another pair of primers the second mutation, the c.3503_3504delTC deletion, was identified. Our findings illustrate that allelic dropout due to the presence of polymorphisms and/or of mutations that trigger the NMD pathway can cause difficulties in current molecular diagnosis tests.M.F. Coutinho is grantee from the FCT (SFRH/BPD/101965/2014).info:eu-repo/semantics/publishedVersio

Laranjeiras 'Valência' enxertadas em híbridos de trifoliata

Rangpur lime is the most important rootstock for the citrus industry of the São Paulo State. Diseases like tristeza, gummosis and declínio/blight have dangeroursly narrowed down the choice for citrus rootstocks. In order to broaden the rootstock avaliability the Centro de Citricultura Sylvio Moreira of the Instituto Agronômico de Campinas maintains a rootstock selection program, consisting specially of Rangpur lime and trifoliate hybrids. This article reports results of an experiment with trifoliate hybrids from the US Horticulture Laboratory in Orlando, Florida. Buds of a nucellar clone of 'Valencia' sweet orange carrying citrus tristeza virus (CTV) were grafted onto trifoliate hybrids. The trees were planted on a sandy textured Oxisol in Pirassununga, SP, near to groves showing declinio/blight and managed without irrigation. Rootstocks in a decreasing order of the yield of eight harvests are: Changsha mandarin ×English Small trifoliate, Sunki mandarin × Benecke trifoliate, Shamouti sweet orange × Mediterranean sweet orange × Christiansen trifoliate, Rangpur lime ×Swingle trifoliate (B), Rangpur lime ×Swingle trifoliate (A) Changsha mandarin × English Large trifoliate, Morton citrange, Miaray mandarin, Siamese × Gotha - road hybrid, Davis A trifoliate, Smooth Flat Seville sour orange × Argentina trifoliate, Troyer and Carrizo tetraployds citranges. The Miaray mandarin showed CTV stem pitting and Smooth Flat Seville ´ Swingle citrumelo hybrid were intolerant to CTV.O limão Cravo é o principal porta-enxerto da citricultura paulista. Sua suscetibilidade ao declínio dos citros e à gomose de Phytophthora reduz a produtividade, onera os tratos culturais e as colheitas e encurta a vida útil dos pomares. É necessário selecionar outros porta-enxertos tolerantes ou resistentes aos fatores bióticos e abióticos limitantes à citricultura paulista. Procuram-se também porta-enxertos nanicantes visando maior produtividade por área, aumento da eficiência e redução nos custos das inspeções e dos controles de pragas e doenças e nas colheitas. Os estudos vem se concentrando na seleção de clones e de híbridos de trifoliata. Laranjeiras 'Valência' enxertadas em híbridos de trifoliata produzidos no USDA foram plantadas em 1986 em Latossolo Vermelho-Amarelo, no município de Pirassununga, SP, próximo a pomares afetados pelo declínio dos citros e conduzidas sem irrigação. Nas oito primeiras colheitas os citrandarins Changsha ×English Small e Sunki × Benecke proporcionaram, em média, as maiores produções em frutos e em sólidos solúveis por planta. As menores produções foram obtidas com os citranges Carrizo e Troyer tetraplóides. A tangerina Miaray e o híbrido Smooth Flat Seville ´ citrumelo Swingle mostraram ser intolerantes à tristeza dos citros

Fabrication, characterization and implementation of thermo resistive TiCu(N,O) thin films in a polymer injection mold

This paper presents the development of metallic thermoresistive thin film, providing an innovative solution to dynamically control the temperature during the injection molding process of polymeric parts. The general idea was to tailor the signal response of the nitrogen- and oxygen-doped titanium-copper thin film (TiCu(N,O))-based transducers, in order to optimize their use in temperature sensor devices. The results reveal that the nitrogen or oxygen doping level has an evident effect on the thermoresistive response of TiCu(N,O) films. The temperature coefficient of resistance values reached 2.29 × 10−2 °C−1, which was almost six times higher than the traditional platinum-based sensors. In order to demonstrate the sensing capabilities of thin films, a proof-of-concept experiment was carried out, integrating the developed TiCu(N,O) films with the best response in an injection steel mold, connected to a data acquisition system. These novel sensor inserts proved to be sensitive to the temperature evolution during the injection process, directly in contact with the polymer melt in the mold, demonstrating their possible use in real operation devices where temperature profiles are a major parameter, such as the injection molding process of polymeric parts.This work was supported by the project SAM—Smart Active Mold (contract ANI—33/SI/2015) by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2019, and from the Basque Government Industry Department under the ELKARTEK and HAZITEK programs

Avaliação das necessidades dos serviços oficiais de saúde pública para o município de São Paulo: a médio e longo prazo

São apresentadas as necessidades de unidades sanitárias a médio e a longo prazo, correspondente aos respectivos anos de 1975 e 1990. Tal estudo integrou o Plano Urbanístico Básico de São Paulo (PUB) e as unidades propostas sempre obedeceram o critério de unidades integradas ou polivalentes. Para definição dos padrões propostos levou-se em conta o tamanho da população, densidade demográfica, tamanho da área, raio de atendimento e classe sócio-econômica. As unidades sanitárias foram divididas em três tipos e definidos, os seus programas de acôrdo com as respectivas atribuições propostas. A responsabilidade funcional foi definida para o setor público municipal, estadual e previdenciário.This paper refers to the demand of sanitary unities, at short and long run, for the years of 1975 and 1990, respectively. This study was part of the "Plano Urbanístico Básico de São Paulo (PUB)", and the proposition always followed the criterium of integrated or polivalent unities. In the definition of the proposed patterns it was considered the size of population, demographic density, size and extension of the area and socio-economic classes. The sanitary unities were divided into three types, and their programs established according to their purposes. The "municipal" authorities are responsible for their functioning, as well as the state and welfare agencies

Effects of wounding, humidity, temperature, and inoculum concentrations on the severity of corky dry rot caused by Fusarium semitectum in melon fruits

[EN] Corky dry rot, caused by Fusarium semitectum, is the main postharvest disease of melons in Brazil. This study investigated the effects of wounding, humidity, temperature, and inoculum concentration on the severity of corky dry rot under controlled conditions. Cantaloupe and honeydew melon types were inoculated by spraying conidial suspensions of three F. semitectum isolates. In all experiments, the tested F. semitectum isolates did not differ in relation to disease severity, but, the cantaloupe melon showed higher levels of severity. No lesions appeared on fruits that lacked wounds, and increasing wound age reduced lesion severity. Melons that were inoculated with F. semitectum developed symptoms regardless of the presence or absence of a moist chamber at the post-inoculation stage, but the lesions were larger under moist chamber conditions. There were no symptoms at 10°C, but a temperature increase from 15 to 25°C resulted in a disease severity increase. The largest lesions were observed when both melon types were inoculated with a concentration of 106 conidia mL-1, but even the lowest concentration (101 conidia mL-1) was sufficient for causing lesions. Injury reduction and/or the acceleration of melon healing, as well as environmental variable control and a reduction of inoculum sources, are essential to reducing corky dry rot severityDe Oliveira, MJ.; Laranjeira, D.; Saraiva Camara, MP.; Ferraz Laranjeira, F.; Armengol Fortí, J.; Michereff, SJ. (2014). Effects of wounding, humidity, temperature, and inoculum concentrations on the severity of corky dry rot caused by Fusarium semitectum in melon fruits. Acta Scientiarum. Agronomy. 36(3):281-289. doi:10.4025/actasciagron.v36i3.17656S28128936

Prevalence, incidence and distribution of citrus variegated chlorosis in Bahia, Brazil

Citrus variegated chlorosis (CVC) is one of the most important diseases for Brazilian citriculture. It is caused by Xylella fastidiosa subsp. pauca, a xylem limited, cycadelid and budwood transmitted bacterium. In Bahia, the second most important citrus region in Brazil, CVC has been present since 1997. Our objectives were to characterize the regional spatial pattern of CVC and to establish a relationship between epidemiological variables and horticultural practices, as well as to evaluate whether control measures used so far have been effective and, based on that, to conceive suitable control measures. A series of surveys were performed in two regions of Bahia State (Recôncavo Baiano and Litoral Norte), along with a survey of horticultural and control practices associated with sampled groves. CVC was restricted to Litoral Norte region, especially to three municipalities along the border between Bahia and Sergipe States. The mean CVC incidence in these municipalities followed a gradient, higher in the countryside and decreasing along the coast. Presence and dissemination of CVC was related to poor nursery practices, a massive use of a susceptible orange variety, and an extreme concentration of orange groves in high incidence municipalities, as well as to the absence of specific CVC control. Considering that CVC was not found in Recôncavo Baiano, this region could be considered a "CVC free zone" by the local government. Copyright by the Brazilian Phytopathological Society.EC/ICA4-CT-2001-10005CNPqFAPES

Structural mechanical simulation to optimize the sensor arm geometry to be implemented on cranial remodeling orthosis

For the treatment of moderate and severe cases of deformational plagiocephaly, an asymmetrical deformation of the skull, a cranial remodeling orthosis (CRO) is used. For the development of a new CRO concept, a pressure sensor grid is placed inside the orthosis that will allow the monitoring of excessive pressures and incorrect CRO positioning throughout the treatment. To implement the sensor grid in the CRO, high mobility of the sensor arms structure that joins the several sensors of this grid is required, however, it is intended that this procedure does not damage the printed copper tracks on the sensor structure. In this study, computer simulations were performed to optimize the sensor arm structure geometry, minimizing undesirable mechanical behavior in the sensor structure when subjected to tensile forces and displacements applied during its placement in the CRO. It was observed that the different sensor arm structures geometries have different effects on the mechanical behavior of the sensor structure when subjected to tensile forces and tensile displacement. The zigzag curve geometry presents the best performance based on high mobility without intense strain on the structure that could damage the printed copper tracks.511F-603F-4B30 | Francisco MirandaN/

Toxicological in vivo studies of an oral insulin nanosystem

http://www.sciencedirect.com/science/article/B6TCR-4PKH640-7G/1/31df064ec957f50aa568d739cd5d741