Precision CW laser automatic tracking system investigated

Precision laser tracker capable of tracking a low acceleration target to an accuracy of about 20 microradians rms is being constructed and tested. This laser tracking has the advantage of discriminating against other optical sources and the capability of simultaneously measuring range

D.7.4 Project Outcome Workshop

To disseminate the results generated by the project and to raise awareness of the needs for standardization for SOC testing and industrialization, the final outcome workshop was organized in the form of a booth at the Hannover Fair (“Hannover Messe”). The test modules were presented, the process followed to arrive at validated procedures and the context in which the protocols operate. Many stakeholders were interviewed, yielding a wide variety of inputs and interpretations. Two oral presentations were given at the Technical Forum in addition to 5-day presence at the booth and promotion of the “SOCTESQA” outcome during all byside events

Collective autobiographical reflexivity on active and compassionate citizenship in the COVID-19 crisis

Since the outbreak of the coronavirus pandemic, socio-economic inequalities have become exacerbated and COVID-19 related hate crimes have increased. This paper explores how citizenship education might be reimagined in response to this context, with the vision of rebuilding a more equitable and compassionate society. By using a collective autobiographical writing approach, this study documented six different autobiographical reflexivities of citizenship education scholars who were from different parts of the world: China, South Korea, the Philippines, the United States, Nepal and the United Kingdom. It also observed the way the pandemic played out in the location where they were situated during the research as well as how it played out in their countries of origin, and further, how it affected the civic development in each context. The scholars' range of autobiographical expressions resulted in insights for developing a type of citizenship education, namely, education for active and compassionate citizenship

SOCTESQA - Solid Oxide Cell and Stack Testing, Safety and Quality Assurance

Many research facilities and industrial companies worldwide are engaged in the development and the improvement of solid oxide fuel cells/stacks (SOFC) and also of solid oxide electrolysis cells/stacks (SOEC). However, the successful application of fuel and electrolysis cells/stacks in real world conditions requires reliable assessment, testing and prediction of performance and durability. Therefore the EU-project SOCTESQA will start at the beginning of May with the aim to develop uniform and industry wide test procedures and protocols for SOC cell/stack assembly. The paper presents the main objectives, the project consortium, the structure, the work packages and the workflow plan of the project. The project builds on experiences gained in the FCTESTNET, FCTESQA series of projects taking up the methodology developed there. It will address new application fields which are based on the operation of the SOFC cell/stack assembly in the fuel cell and in the electrolysis mode, e.g. stationary SOFC μ-CHP, mobile SOFC APU and SOFC/SOEC power-to-gas systems. The test procedures will include current-voltage curves, electrochemical impedance spectroscopy and long term tests both under steady state and dynamic operating conditions. The project partners are from different countries in Europe: French Alternative Energies and Atomic Energy Commission (CEA), Technical University of Denmark (DTU), Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Joint Research Centre – European Commission (JRC) from Belgium, European Institute for Energy Research (EIFER) from Germany and German Aerospace Center (DLR). All of them have long-term experience in the development, testing and harmonization of solid oxide cells/stacks. The project will have a clear structure based on an initial definition phase, the development of generic test modules, the corresponding experimental validation phases and the review of the test procedures. Several of these validation loops will result at the end of the project in final test modules, which will be confirmed by round robin tests. Moreover, the project will address safety aspects, liaise with standardization organizations and establish contact with industrial practice. This collaborative project will essentially help to accelerate the development and the market penetration of hydrogen and fuel cell (H2&FC) energy systems in Europe

Impact of an interatrial shunt device on survival and heart failure hospitalization in patients with preserved ejection fraction

Aims: Impaired left ventricular diastolic function leading to elevated left atrial pressures, particularly during exertion, is a key driver of symptoms and outcomes in heart failure with preserved ejection fraction (HFpEF). Insertion of an interatrial shunt device (IASD) to reduce left atrial pressure in HFpEF has been shown to be associated with short‐term haemodynamic and symptomatic benefit. We aimed to investigate the potential effects of IASD placement on HFpEF survival and heart failure hospitalization (HFH). Methods and results: Heart failure with preserved ejection fraction patients participating in the Reduce Elevated Left Atrial Pressure in Patients with Heart Failure study (Corvia Medical) of an IASD were followed for a median duration of 739 days. The theoretical impact of IASD implantation on HFpEF mortality was investigated by comparing the observed survival of the study cohort with the survival predicted from baseline data using the Meta‐analysis Global Group in Chronic Heart Failure heart failure risk survival score. Baseline and post‐IASD implant parameters associated with HFH were also investigated. Based upon the individual baseline demographic and cardiovascular profile of the study cohort, the Meta‐analysis Global Group in Chronic Heart Failure score‐predicted mortality was 10.2/100 pt years. The observed mortality rate of the IASD‐treated cohort was 3.4/100 pt years, representing a 33% lower rate (P = 0.02). By Kaplan–Meier analysis, the observed survival in IASD patients was greater than predicted (P = 0.014). Baseline parameters were not predictive of future HFH events; however, poorer exercise tolerance and a higher workload‐corrected exercise pulmonary capillary wedge pressure at the 6 months post‐IASD study were associated with HFH. Conclusions: The current study suggests IASD implantation may be associated with a reduction in mortality in HFpEF. Large‐scale ongoing randomized studies are required to confirm the potential benefit of this therapy

D.3.1 Test Matrix Document

The present document defines the test matrix, i.e. a list of test modules relevant for different applications. According to the project objectives the applications are SOFC (stationary and mobile), SOEC (H2- production) and combined SOFC/SOEC (electricity storage via H2). These test modules can be combined to form test programs in order to realize application-oriented testing. This test matrix has been created based on a brief review of results from the precedent project - FCTESQA dealing with cell/stack/system testing procedures for three types of fuel cells (PEMFC, SOFC and MCFC) and the on-going project STACKTEST dealing with testing procedures for PEMFC stacks. Industrial stake holders who are developing SOFC/SOEC products have been contacted to gather information regarding the required operation modes during the lifecycle of the product for each application. Feedbacks from industrial stake holders have also been integrated

The role of TcdB and TccC subunits in secretion of the photorhabdus Tcd toxin complex

The Toxin Complex (TC) is a large multi-subunit toxin encoded by a range of bacterial pathogens. The best-characterized examples are from the insect pathogens Photorhabdus, Xenorhabdus and Yersinia. They consist of three large protein subunits, designated A, B and C that assemble in a 5:1:1 stoichiometry. Oral toxicity to a range of insects means that some have the potential to be developed as pest control technology. The three subunit proteins do not encode any recognisable export sequences and as such little progress has been made in understanding their secretion. We have developed heterologous TC production and secretion models in E. coli and used them to ascribe functions to different domains of the crucial B+C sub-complex. We have determined that the B and C subunits use a secretion mechanism that is either encoded by the proteins themselves or employ an as yet undefined system common to laboratory strains of E. coli. We demonstrate that both the N-terminal domains of the B and C subunits are required for secretion of the whole complex. We propose a model whereby the N-terminus of the C-subunit toxin exports the B+C sub-complex across the inner membrane while that of the B-subunit allows passage across the outer membrane. We also demonstrate that even in the absence of the B-subunit, that the C-subunit can also facilitate secretion of the larger A-subunit. The recognition of this novel export system is likely to be of importance to future protein secretion studies. Finally, the identification of homologues of B and C subunits in diverse bacterial pathogens, including Burkholderia and Pseudomonas, suggests that these toxins are likely to be important in a range of different hosts, including man

A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative type IV secretion system

Type IV secretion system (T4SS) substrates are recruited through a translocation signal that is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is translocated by its cognate conjugative T4SS, and it can also be translocated by the VirB/D4 T4SS of Bartonella henselae, causing DNA transfer to human cells. In this work, we constructed a series of TrwC variants and assayed them for DNA transfer to bacteria and human cells to compare recruitment requirements by both T4SSs. Comparison with other reported relaxase translocation signals allowed us to determine two putative translocation sequence (TS) motifs, TS1 and TS2. Mutations affecting TS1 drastically affected conjugation frequencies, while mutations affecting either motif had only a mild effect on DNA transfer rates through the VirB/D4 T4SS of B. henselae. These results indicate that a single substrate can be recruited by two different T4SSs through different signals. The C terminus affected DNA transfer rates through both T4SSs tested, but no specific sequence requirement was detected. The addition of a Bartonella intracellular delivery (BID) domain, the translocation signal for the Bartonella VirB/D4 T4SS, increased DNA transfer up to 4% of infected human cells, providing an excellent tool for DNA delivery to specific cell types. We show that the R388 coupling protein TrwB is also required for this high-efficiency TrwC-BID translocation. Other elements apart from the coupling protein may also be involved in substrate recognition by T4SSs

Resuscitation Endpoints in Trauma

Fluid and blood resuscitation is the mainstay of therapy for the treatment of hemorrhagic shock, whether due to trauma or other etiology. Cessation of hemorrhage with rapid hemostatic techniques is the first priority in the treatment of traumatic hemorrhagic shock, with concomitant fluid resuscitation with blood and crystalloids to maintain perfusion and organ function. “Hypotensive” or “low-volume” resuscitation has become increasingly accepted in the prehospital resuscitation phase of trauma, prior to definitive hemorrhage control, since aggressive fluid resuscitation may increase bleeding. Resuscitation after hemorrhage control is focused on restoration of tissue oxygenation. Efforts to optimize resuscitation have used “resuscitation endpoints” as markers of adequacy of resuscitation. The resuscitation endpoints that have been evaluated include both global (restoration of blood pressure, heart rate and urine output, lactate, base deficit, mixed venous oxygen saturation, ventricular end-diastolic volume) and regional (gastric tonometry, near-infrared spectroscopy for measurement of muscle tissue oxygen saturation) measures. This review critically evaluates the evidence regarding the use of resuscitation endpoints in trauma.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75386/1/j.1778-428X.2005.tb00127.x.pd