The Frog Vestibular System as a Model for Lesion-Induced Plasticity: Basic Neural Principles and Implications for Posture Control

Studies of behavioral consequences after unilateral labyrinthectomy have a long tradition in the quest of determining rules and limitations of the central nervous system (CNS) to exert plastic changes that assist the recuperation from the loss of sensory inputs. Frogs were among the first animal models to illustrate general principles of regenerative capacity and reorganizational neural flexibility after a vestibular lesion. The continuous successful use of the latter animals is in part based on the easy access and identifiability of nerve branches to inner ear organs for surgical intervention, the possibility to employ whole brain preparations for in vitro studies and the limited degree of freedom of postural reflexes for quantification of behavioral impairments and subsequent improvements. Major discoveries that increased the knowledge of post-lesional reactive mechanisms in the CNS include alterations in vestibular commissural signal processing and activation of cooperative changes in excitatory and inhibitory inputs to disfacilitated neurons. Moreover, the observed increase of synaptic efficacy in propriospinal circuits illustrates the importance of limb proprioceptive inputs for postural recovery. Accumulated evidence suggests that the lesion-induced neural plasticity is not a goal-directed process that aims toward a meaningful restoration of vestibular reflexes but rather attempts a survival of those neurons that have lost their excitatory inputs. Accordingly, the reaction mechanism causes an improvement of some components but also a deterioration of other aspects as seen by spatio-temporally inappropriate vestibulo-motor responses, similar to the consequences of plasticity processes in various sensory systems and species. The generality of the findings indicate that frogs continue to form a highly amenable vertebrate model system for exploring molecular and physiological events during cellular and network reorganization after a loss of vestibular function

Power laws in microrheology experiments on living cells: comparative analysis and modelling

We compare and synthesize the results of two microrheological experiments on the cytoskeleton of single cells. In the first one, the creep function J(t) of a cell stretched between two glass plates is measured after applying a constant force step. In the second one, a micrometric bead specifically bound to transmembrane receptors is driven by an oscillating optical trap, and the viscoelastic coefficient $G_e(\omega)$ is retrieved. Both $J(t)$ and $G_e(\omega)$ exhibit power law behavior: $J(t)= A(t/t_0)^\alpha$ and $\bar G_e(\omega)\bar = G_0 (\omega/\omega_0)^\alpha$, with the same exponent $\alpha\approx 0.2$. This power law behavior is very robust ; $\alpha$ is distributed over a narrow range, and shows almost no dependance on the cell type, on the nature of the protein complex which transmits the mechanical stress, nor on the typical length scale of the experiment. On the contrary, the prefactors $A_0$ and $G_0$appear very sensitive to these parameters. Whereas the exponents $\alpha$ are normally distributed over the cell population, the prefactors $A_0$ and $G_0$ follow a log-normal repartition. These results are compared with other data published in the litterature. We propose a global interpretation, based on a semi-phenomenological model, which involves a broad distribution of relaxation times in the system. The model predicts the power law behavior and the statistical repartition of the mechanical parameters, as experimentally observed for the cells. Moreover, it leads to an estimate of the largest response time in the cytoskeletal network: $\tau_m \approx 1000$ s.Comment: 47 pages, 14 figures // v2: PDF file is now Acrobat Reader 4 (and up) compatible // v3: Minor typos corrected - The presentation of the model have been substantially rewritten (p. 17-18), in order to give more details - Enhanced description of protocols // v4: Minor corrections in the text : the immersion angles are estimated and not measured // v5: Minor typos corrected. Two references were clarifie

Dual function of thalamic low-vigilance state oscillations: Rhythm-regulation and plasticity

During inattentive wakefulness and non-rapid eye movement (NREM) sleep, the neocortex and thalamus cooperatively engage in rhythmic activities that are exquisitely reflected in the electroencephalogram as distinctive rhythms spanning a range of frequencies from <1 Hz slow waves to 13 Hz alpha waves. In the thalamus, these diverse activities emerge through the interaction of cell-intrinsic mechanisms and local and long-range synaptic inputs. One crucial feature, however, unifies thalamic oscillations of different frequencies: repetitive burst firing driven by voltage-dependent Ca(2+) spikes. Recent evidence reveals that thalamic Ca(2+) spikes are inextricably linked to global somatodendritic Ca(2+) transients and are essential for several forms of thalamic plasticity. Thus, we propose herein that alongside their rhythm-regulation function, thalamic oscillations of low-vigilance states have a plasticity function that, through modifications of synaptic strength and cellular excitability in local neuronal assemblies, can shape ongoing oscillations during inattention and NREM sleep and may potentially reconfigure thalamic networks for faithful information processing during attentive wakefulness

Sulphur abundance in Galactic stars

We investigate sulphur abundance in 74 Galactic stars by using high resolution spectra obtained at ESO VLT and NTT telescopes. For the first time the abundances are derived, where possible, from three optical multiplets: Mult. 1, 6, and 8. By combining our own measurements with data in the literature we assemble a sample of 253 stars in the metallicity range -3.2 < [Fe/H] < +0.5. Two important features, which could hardly be detected in smaller samples, are obvious from this large sample: 1) a sizeable scatter in [S/Fe] ratios around [Fe/H] ~ -1 ; 2) at low metallicities we observe stars with [S/Fe] ~ 0.4, as well as stars with higher [S/Fe] ratios. The latter do not seem to be kinematically different from the former ones. Whether the latter finding stems from a distinct population of metal-poor stars or simply from an increased scatter in sulphur abundances remains an open question.Comment: accepted by A&

Sulphur and zinc abundances in Galactic stars and damped Lyman-alpha systems

High resolution spectra of 34 halo population dwarf and subgiant stars have been obtained with VLT/UVES and used to derive sulphur abundances from the 8694.0, 8694.6 A and 9212.9, 9237.5 A SI lines. In addition, iron abundances have been determined from 19 FeII lines and zinc abundances from the 4722.2, 4810.5 ZnI lines. The abundances are based on a classical 1D, LTE model atmosphere analysis, but effects of 3D hydrodynamical modelling on the [S/Fe], [Zn/Fe] and [S/Zn] ratios are shown to be small. We find that most halo stars with metallicities in the range -3.2 < [Fe/H] < -0.8 have a near-constant [S/Fe] = +0.3; a least square fit to [S/Fe] vs. {Fe/H] shows a slope of only -0.04 +/- 0.01. Among halo stars with -1.2 < [Fe/H] < -0.8 the majority have [S/Fe] ~ +0.3, but two stars (previously shown to have low [alpha/Fe] ratios) have [S/Fe] ~ 0. For disk stars with [Fe/H] > -1, [S/Fe] decreases with increasing [Fe/H]. Hence, sulphur behaves like other typical alpha-capture elements, Mg, Si and Ca. Zinc, on the other hand, traces iron over three orders of magnitude in [Fe/H], although there is some evidence for a small systematic Zn overabundance ([Zn/Fe] ~ +0.1) among metal-poor disk stars and for halo stars with [Fe/H] < -2.0. Recent measurements of S and Zn in ten damped Ly-alpha systems (DLAs) with redshifts between 1.9 and 3.4 and zinc abundances in the range -2.1 < [Zn/H] < -0.15 show an offset relative to the [S/Zn] - [Zn/H] relation in Galactic stars. Possible reasons for this offset are discussed, including low and intermittent star formation rates in DLAs.Comment: Accepted for publication in A&A. 16pages; 9 figure

V453 Oph: a s-process enriched, but carbon-deficient RV Tauri star of low intrinsic metallicity

This paper reports the detection of a heavy element enriched RV Tauri variable with an abundance pattern that differs significantly from a standard s-process enriched object: V453 Oph. Based on optical high-resolution spectra, we determined that this object of low intrinsic metallicity ([Fe/H] = -2.2) has a mild, but significant, enrichment ([s/Fe] ~ +0.5) of heavy elements for which the distribution points to slow neutron capture nucleosynthesis. This result is strengthened by a comparative analysis to the non-enriched RV Tauri star DS Aqr ([s/Fe] = 0.0). Although V453 Oph is the first RV Tauri star showing a strong s-process signature, it is NOT accompanied by C enhancement, challenging our current nucleosynthetic models of post-AGB stars that predict a simultaneous enrichment in C and s-process elements. The low N abundance excludes CN cycling as being responsible for the low C abundance. We explore three different scenarios to explain the heavy element distribution in this evolved object: an enrichment of the parental cloud, an accretion scenario in which the chemical patterns were acquired by mass transfer in a binary system and an intrinsic enrichment by dredge-up.Comment: Accepted for publication in A&

Intersection rules, dynamics and symmetries

We consider theories containing gravity, at most one dilaton and form field strengths. We show that the existence of particular BPS solutions of intersecting extremal closed branes select the theories, which upon dimensional reduction to three dimensions possess a simple simply laced Lie group symmetry G. Furthermore these theories can be fully reconstructed from the dynamics of such branes and of their openings. Amongst such theories are the effective actions of the bosonic sector of M-theory and of the bosonic string. The BPS intersecting brane solutions form representations of a subgroup of the group of Weyl reflections and outer automorphisms of the triple Kac-Moody extension G+++ of the G algebra, which cannot be embedded in the overextended Kac-Moody subalgebra G++ characterising the cosmological Kasner solutions.Comment: Latex 30 pages, 3 figure

Analysis of 26 Barium Stars I. Abundances

We present a detailed analysis of 26 barium stars, including dwarf barium stars, providing their atmospheric parameters (Teff, log g, [Fe/H], vt) and elemental abundances. We aim at deriving gravities and luminosity classes of the sample stars, in particular to confirm the existence of dwarf barium stars. Accurate abundances of chemical elements were derived. Abundance ratios between nucleosynthetic processes, by using Eu and Ba as representatives of the r- and s-processes are presented. High-resolution spectra with the FEROS spectrograph at the ESO-1.5m Telescope, and photometric data with Fotrap at the Zeiss telescope at the LNA were obtained. The atmospheric parameters were derived in an iterative way, with temperatures obtained from colour-temperature calibrations. The abundances were derived using spectrum synthesis for Li, Na, Al, alpha-, iron peak, s- and r-elements atomic lines, and C and N molecular lines. Atmospheric parameters in the range 4300 < Teff < 6500, -1.2 < [Fe/H] < 0.0 and 1.4 < log g < 4.6 were derived, confirming that our sample contains giants, subgiants and dwarfs. The abundance results obtained for Li, Al, Na, alpha- and iron peak elements for the sample stars show that they are compatible with the values found in the literature for normal disk stars in the same range of metallicities. Enhancements of C, N and heavy elements relative to Fe, that characterise barium stars, were derived and showed that [X/Ba] vs. [Ba/H] and [X/Ba] vs. [Fe/H] present different behaviour as compared to [X/Eu] vs. [Eu/H] and [X/Eu] vs. [Fe/H], reflecting the different nucleosynthetic sites for the s- and r-processes.Comment: 32 pages including 18 figures, accepted to A&