Search for new strategies for controlling viral diseases in aquaculture

A enfermidade que causa maiores perdas económicas na acuicultura europea de robaliza é a retinopatía e encefalopatía vírica, cuxo axente causante é o nodavirus. O obxectivo desta tese de doutoramento foi profundar no coñecemento da resposta a unha infección por nodavirus. O uso de ferramentas bioinformáticas permítenos estudar con maior profundidade as interaccións entre o hóspede e o patóxeno, para o que se analizou a resposta transcriptómica completa da robaliza europea ao nodavirus coa técnica RNA-Seq, así como a súa posible modulación por ARNs non codificantes do xenoma (lncRNAs). Tamén se desenvolveu un prototipo vacina usando spynicterins, mellorando a seguridade e a rendibilidade ambiental ao non requirir adxuvantes. A relativa dificultade para traballar con especies de alto valor comercial levounos a establecer o peixe cebra como modelo de infección por nodavirus

BIENESTAR EN REPTILES EN CAUTIVIDAD: ASPECTOS BÁSICOS Y REPORTES DE CASOS

La tenencia de reptiles en cautividad se ha incrementado en los últimos años, al mismo tiempo, su población silvestre cada vez se ve más disminuida en la naturaleza. Muchas de las especies que se comercializan están protegidas, y las condiciones de captura y de exportación les están llevando al borde de la extinción. Los reptiles atraen la atención de muchos potenciales propietarios pero la gran mayoría no sabe cómo mantenerlos en condiciones de cautiverio, ni las necesidades a cubrirpara cada especie. Las principales causas de problemas de salud en reptiles en cautiverio están relacionadas con las condiciones inadecuadas de cría y manejo de los mismos. El objetivo general de este trabajo está dirigido a hacer una revisión bibliográfica sobre aspectos generales del mantenimiento de reptiles en cautividad y las principales enfermedades derivadas de un manejo inadecuado. Reflexionar sobre las graves consecuencias que para muchas especies exóticas conlleva su comercio ilegal o incontrolado. Además, se presentan tres estudios de casos clínicos relacionados con problemas de salud y bienestar detectados durante la estancia de la autora en el hospital veterinario de la Universidad de Medicina Veterinaria y Farmacia en Kosice.<br /

3D-printed PLA medical devices: physicochemical changes and biological response after sterilisation treatments

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGPolylactic acid (PLA) has become one of the most commonly used polymers in medical devices given its biocompatible, biodegradable and bioabsorbable properties. In addition, due to PLA’s thermoplastic behaviour, these medical devices are now obtained using 3D printing technologies. Once obtained, the 3D-printed PLA devices undergo different sterilisation procedures, which are essential to prevent infections. This work was an in-depth study of the physicochemical changes caused by novel and conventional sterilisation techniques on 3D-printed PLA and their impact on the biological response in terms of toxicity. The 3D-printed PLA physicochemical (XPS, FTIR, DSC, XRD) and mechanical properties as well as the hydrophilic degree were evaluated after sterilisation using saturated steam (SS), low temperature steam with formaldehyde (LTSF), gamma irradiation (GR), hydrogen peroxide gas plasma (HPGP) and CO2 under critical conditions (SCCO). The biological response was tested in vitro (fibroblasts NCTC-929) and in vivo (embryos and larvae wild-type zebrafish Danio rerio). The results indicated that after GR sterilisation, PLA preserved the O:C ratio and the semi-crystalline structure. Significant changes in the polymer surface were found after HPGP, LTSF and SS sterilisations, with a decrease in the O:C ratio. Moreover, the FTIR, DSC and XRD analysis revealed PLA crystallisation after SS sterilisation, with a 52.9% increase in the crystallinity index. This structural change was also reflected in the mechanical properties and wettability. An increase in crystallinity was also observed after SCCO and LTSF sterilisations, although to a lesser extent. Despite these changes, the biological evaluation revealed that none of the techniques were shown to promote the release of toxic compounds or PLA modifications with toxicity effects. GR sterilisation was concluded as the least reactive technique with good perspectives in the biological response, not only at the level of toxicity but at all levels, since the 3D-printed PLA remained almost unaltered.POCTEP INTERREG España- Portugal | Ref. BLUEBIOLABInterreg Atlantic Area | Ref. BLUEHUMAN EAPA_151/2016Ministerio de Ciencia e Innovación | Ref. PID 2020-115415RB-100Xunta de Galicia | Ref. ED431C 2021/49Xunta de Galicia | Ref. ED481A 2019/314Xunta de Galicia | Ref. IN606A-2017/011Fundação para a Ciência e a Tecnologia | Ref. UIDB/50016/202

Risk Factors for COVID-19 in Inflammatory Bowel Disease: A National, ENEIDA-Based Case–Control Study (COVID-19-EII)

(1) Scant information is available concerning the characteristics that may favour the acquisition of COVID-19 in patients with inflammatory bowel disease (IBD). Therefore, the aim of this study was to assess these differences between infected and noninfected patients with IBD. (2) This nationwide case-control study evaluated patients with inflammatory bowel disease with COVID-19 (cases) and without COVID-19 (controls) during the period March-July 2020 included in the ENEIDA of GETECCU. (3) A total of 496 cases and 964 controls from 73 Spanish centres were included. No differences were found in the basal characteristics between cases and controls. Cases had higher comorbidity Charlson scores (24% vs. 19%; p = 0.02) and occupational risk (28% vs. 10.5%; p < 0.0001) more frequently than did controls. Lockdown was the only protective measure against COVID-19 (50% vs. 70%; p < 0.0001). No differences were found in the use of systemic steroids, immunosuppressants or biologics between cases and controls. Cases were more often treated with 5-aminosalicylates (42% vs. 34%; p = 0.003). Having a moderate Charlson score (OR: 2.7; 95%CI: 1.3-5.9), occupational risk (OR: 2.9; 95%CI: 1.8-4.4) and the use of 5-aminosalicylates (OR: 1.7; 95%CI: 1.2-2.5) were factors for COVID-19. The strict lockdown was the only protective factor (OR: 0.1; 95%CI: 0.09-0.2). (4) Comorbidities and occupational exposure are the most relevant factors for COVID-19 in patients with IBD. The risk of COVID-19 seems not to be increased by immunosuppressants or biologics, with a potential effect of 5-aminosalicylates, which should be investigated further and interpreted with caution

Search for new strategies for controlling viral diseases in aquaculture

A enfermidade que causa maiores perdas económicas na acuicultura europea de robaliza é a retinopatía e encefalopatía vírica, cuxo axente causante é o nodavirus. O obxectivo desta tese de doutoramento foi profundar no coñecemento da resposta a unha infección por nodavirus. O uso de ferramentas bioinformáticas permítenos estudar con maior profundidade as interaccións entre o hóspede e o patóxeno, para o que se analizou a resposta transcriptómica completa da robaliza europea ao nodavirus coa técnica RNA-Seq, así como a súa posible modulación por ARNs non codificantes do xenoma (lncRNAs). Tamén se desenvolveu un prototipo vacina usando spynicterins, mellorando a seguridade e a rendibilidade ambiental ao non requirir adxuvantes. A relativa dificultade para traballar con especies de alto valor comercial levounos a establecer o peixe cebra como modelo de infección por nodavirus

Actualización del cuestionario benchmarcking de ASLOG sobre la vanguardia logística en Francia y el extranjero : (2011-2012)

Ce rapport présente le travail effectué pendant 4 mois pour la réalisation du challenge de l’enquête Benchmarking de l’ASLOG menée tous les 2 ans. Cette étude a pour but d’établir les avis et les attentes des entreprises adhérentes à l’ASLOG ainsi que des Associations Logistiques étrangères sur l’état de l’art de la logistique et de la Supply Chain. Notre mission, limitée à interviewer aux entreprises d’Ile de France, était dans un premier temps de réaliser un maximum d’interviews au sein des entreprises auprès de responsables logistiques ou Supply Chain, pour réaliser le challenge du questionnaire Benchmarking. Dans un second temps, nous avons réalisé cette même démarche via mailing avec les Associations Logistiques étrangères. Après de nombreux mails, nous avons réussi à avoir des résultats qui nous ont permis d’effectuer une analyse sur les attentes actuelles de la logistique.Ingeniería Industria