Effects of Genetic Captive-Breeding Protocols on Sperm Quality and Fertility in the White-Footed Mouse

P. 540–548Mice (Peromyscus leucopus noveboracensis) from a captive-breeding program were used to test the effects of three genetic breeding protocols (minimizing mean kinship [MK], random breeding, and selection for docility [DOC]) and inbreeding levels on sperm traits and fertility. Earlier, in generation 8, one DOC replicate went extinct because of poor reproductive success. By generation 10, spermatozoa from DOC mice had more acrosome and midpiece abnormalities, which were shown to be strong determinants of fertility, as well as lower sperm production and resistance to osmotic stress. In addition, determinants of fertility, including male and female components, were assessed in a comprehensive manner. Results showed that the probability (P) of siring litters is determined by sperm number, sperm viability, and midpiece and acrosome abnormalities; that the P of siring one versus two litters is determined by tail abnormalities; and that the total number of offspring is influenced by female size and proportion of normal sperm, showing the relative importance of different sperm traits on fertility. On average, males with 20% normal sperm sired one pup per litter, and males with 70% normal sperm sired eight pups per litter. Interestingly, the proportion of normal sperm was affected by docility but not by relatively low inbreeding. However, inbreeding depression in sperm motility was detected. In the MK group, inbreeding depression not only affected sperm motility but also fertility: An increase in the coefficient of inbreeding (f) of 0.03 reduced sperm motility by 30% and translated into an offspring reduction of three pups in second litters. A genetic load of 48 fecundity equivalents was calculated.S

Eight-Dimensional Mid-Infrared/Optical Bayesian Quasar Selection

We explore the multidimensional, multiwavelength selection of quasars from mid-IR (MIR) plus optical data, specifically from Spitzer-IRAC and the Sloan Digital Sky Survey (SDSS). We apply modern statistical techniques to combined Spitzer MIR and SDSS optical data, allowing up to 8-D color selection of quasars. Using a Bayesian selection method, we catalog 5546 quasar candidates to an 8.0 um depth of 56 uJy over an area of ~24 sq. deg; ~70% of these candidates are not identified by applying the same Bayesian algorithm to 4-color SDSS optical data alone. Our selection recovers 97.7% of known type 1 quasars in this area and greatly improves the effectiveness of identifying 3.5<z<5 quasars. Even using only the two shortest wavelength IRAC bandpasses, it is possible to use our Bayesian techniques to select quasars with 97% completeness and as little as 10% contamination. This sample has a photometric redshift accuracy of 93.6% (Delta Z +/-0.3), remaining roughly constant when the two reddest MIR bands are excluded. While our methods are designed to find type 1 (unobscured) quasars, as many as 1200 of the objects are type 2 (obscured) quasar candidates. Coupling deep optical imaging data with deep mid-IR data could enable selection of quasars in significant numbers past the peak of the quasar luminosity function (QLF) to at least z~4. Such a sample would constrain the shape of the QLF and enable quasar clustering studies over the largest range of redshift and luminosity to date, yielding significant gains in our understanding of quasars and the evolution of galaxies.Comment: 49 pages, 14 figures, 7 tables. AJ, accepte

Evaluating anthropogenic threats to endangered killer whales to inform effective recovery plans

Understanding cumulative effects of multiple threats is key to guiding effective management to conserve endangered species. The critically endangered, Southern Resident killer whale population of the northeastern Pacific Ocean provides a data-rich case to explore anthropogenic threats on population viability. Primary threats include: limitation of preferred prey, Chinook salmon; anthropogenic noise and disturbance, which reduce foraging efficiency; and high levels of stored contaminants, including PCBs. We constructed a population viability analysis to explore possible demographic trajectories and the relative importance of anthropogenic stressors. The population is fragile, with no growth projected under current conditions, and decline expected if new or increased threats are imposed. Improvements in fecundity and calf survival are needed to reach a conservation objective of 2.3% annual population growth. Prey limitation is the most important factor affecting population growth. However, to meet recovery targets through prey management alone, Chinook abundance would have to be sustained near the highest levels since the 1970s. The most optimistic mitigation of noise and contaminants would make the difference between a declining and increasing population, but would be insufficient to reach recovery targets. Reducing acoustic disturbance by 50% combined with increasing Chinook by 15% would allow the population to reach 2.3% growth

The Massive Hosts of Radio Galaxies Across Cosmic Time

We present the results of a comprehensive Spitzer survey of 69 radio galaxies across 1<z<5.2. Using IRAC (3.6-8.0um), IRS (16um) and MIPS (24-160um) imaging, we decompose the rest-frame optical to infrared spectral energy distributions into stellar, AGN, and dust components and determine the contribution of host galaxy stellar emission at rest-frame H-band. Stellar masses derived from rest-frame near-IR data, where AGN and young star contributions are minimized, are significantly more reliable than those derived from rest-frame optical and UV data. We find that the fraction of emitted light at rest-frame H-band from stars is >60% for ~75% the high redshift radio galaxies. As expected from unified models of AGN, the stellar fraction of the rest-frame H-band luminosity has no correlation with redshift, radio luminosity, or rest-frame mid-IR (5um) luminosity. Additionally, while the stellar H-band luminosity does not vary with stellar fraction, the total H-band luminosity anti-correlates with the stellar fraction as would be expected if the underlying hosts of these radio galaxies comprise a homogeneous population. The resultant stellar luminosities imply stellar masses of 10^{11-11.5}Msun even at the highest redshifts. Powerful radio galaxies tend to lie in a similar region of mid-IR color-color space as unobscured AGN, despite the stellar contribution to their mid-IR SEDs at shorter-wavelengths. The mid-IR luminosities alone classify most HzRGs as LIRGs or ULIRGs with even higher total-IR luminosities. As expected, these exceptionally high mid-IR luminosities are consistent with an obscured, highly-accreting AGN. We find a weak correlation of stellar mass with radio luminosity.Comment: 63 pages, 14 figures, accepted for publication in ApJ

Gene content evolution in the arthropods

Arthropods comprise the largest and most diverse phylum on Earth and play vital roles in nearly every ecosystem. Their diversity stems in part from variations on a conserved body plan, resulting from and recorded in adaptive changes in the genome. Dissection of the genomic record of sequence change enables broad questions regarding genome evolution to be addressed, even across hyper-diverse taxa within arthropods. Using 76 whole genome sequences representing 21 orders spanning more than 500 million years of arthropod evolution, we document changes in gene and protein domain content and provide temporal and phylogenetic context for interpreting these innovations. We identify many novel gene families that arose early in the evolution of arthropods and during the diversification of insects into modern orders. We reveal unexpected variation in patterns of DNA methylation across arthropods and examples of gene family and protein domain evolution coincident with the appearance of notable phenotypic and physiological adaptations such as flight, metamorphosis, sociality, and chemoperception. These analyses demonstrate how large-scale comparative genomics can provide broad new insights into the genotype to phenotype map and generate testable hypotheses about the evolution of animal diversity

No effect of ascorbate on cutaneous vasodilation and sweating in older men and those with type 2 diabetes exercising in the heat

Aging and chronic disease such as type 2 diabetes (T2D) are associated with impairments in the body\u27s ability to dissipate heat. To reduce the risk of heat‐related injuries in these heat vulnerable individuals, it is necessary to identify interventions that can attenuate this impairment. We evaluated the hypothesis that intradermal administration of ascorbate improves cutaneous vasodilation and sweating in older adults via nitric oxide synthase (NOS)‐dependent mechanisms during exercise in the heat and whether these improvements, if any, are greater in individuals with T2D. Older males with (n = 12, 61 ± 9 years) and without (n = 12, 64 ± 7 years) T2D performed two 30‐min bouts of cycling at a fixed rate of metabolic heat production of 500 W (~70% peak oxygen uptake) in the heat (35°C); each followed by a 20‐ and 40‐min recovery, respectively. Cutaneous vascular conductance (CVC) and sweat rate were measured at four intradermal microdialysis sites treated with either (1) lactated Ringer (Control), (2) 10 mmol/L ascorbate (an antioxidant), (3) 10 mmol/L L‐NAME (non‐selective NOS inhibitor), or (4) a combination of ascorbate + L‐NAME. In both groups, ascorbate did not modulate CVC or sweating during exercise relative to Control (all P > 0.05). In comparison to Control, L‐NAME alone or combined with ascorbate attenuated CVC during exercise (all P ≤ 0.05) but had no influence on sweating (all P > 0.05). We show that in both healthy and T2D older adults, intradermal administration of ascorbate does not improve cutaneous vasodilation and sweating during exercise in the heat. However, NOS plays an important role in mediating cutaneous vasodilation

High-Redshift Galaxy Outflows and the Formation of Dwarf Galaxies

We examine the effects of galaxy outflows on the formation of dwarf galaxies in numerical simulations of the high-redshift Universe. Using a Smoothed Particle Hydrodynamic code, we conduct two detailed simulations of a (5.2 Mpc/h)^3 comoving volume of the Universe. In both simulations we implement simple, well-motivated models of galaxy identification and star formation, while our second simulation also includes a simple ``blow-out'' model of galaxy outflows in which supernova driven winds from newly formed disk galaxies punch-out and shock the intergalactic medium while leaving the host galaxies intact. A direct comparison between these simulations suggests that there are two major mechanisms by which outflows affect dwarf formation. Firstly, the formation of an outflow slows down the further accretion of gas onto a galaxy, causing an overall decrease of approximately 50% in the total gas mass accreted by the objects in our simulations. Additionally, our simulations uncover a significant population of approximately 10^9 solar mass objects whose formation is suppressed by the mechanism of ``baryonic stripping,'' in which outflows from early galaxies strip the gas out of nearby overdense regions that would have otherwise later formed into dwarf galaxies. This mechanism may be important in explaining the observed discrepancy between the number of dwarf galaxies predicted and observed in the local group and provide a natural explanation for the formation of empty halos which may be required by the existence of the extremely gas-poor extra-galactic High-Velocity Clouds.Comment: 15 pages, 9 figures, accepted to the Astrophysical Journa

Impacts of urbanisation on the native avifauna of Perth, Western Australia

Urban development either eliminates, or severely fragments, native vegetation, and therefore alters the distribution and abundance of species that depend on it for habitat. We assessed the impact of urban development on bird communities at 121 sites in and around Perth, Western Australia. Based on data from community surveys, at least 83 % of 65 landbirds were found to be dependent, in some way, on the presence of native vegetation. For three groups of species defined by specific patterns of habitat use (bushland birds), there were sufficient data to show that species occurrences declined as the landscape changed from variegated to fragmented to relictual, according to the percentage of vegetation cover remaining. For three other groups (urban birds) species occurrences were either unrelated to the amount of vegetation cover, or increased as vegetation cover declined. In order to maximise the chances of retaining avian diversity when planning for broad-scale changes in land-use (i.e. clearing native vegetation for housing or industrial development), land planners should aim for a mosaic of variegated urban landscapes (\u3e60 % vegetation retention) set amongst the fragmented and relictual urban landscapes (% vegetation retention) that are characteristic of most cities and their suburbs. Management actions for conserving remnant biota within fragmented urban landscapes should concentrate on maintaining the integrity and quality of remnant native vegetation, and aim at building awareness among the general public of the conservation value of remnant native vegetation

Safety and pharmacokinetics of multiple dose myo-inositol in preterm infants

BACKGROUND: Preterm infants with respiratory distress syndrome (RDS) given inositol had reduced bronchopulmonary dysplasia (BPD), death and severe retinopathy of prematurity (ROP). We assessed the safety and pharmacokinetics of daily inositol to select a dose providing serum levels previously associated with benefit, and to learn if accumulation occurred when administered throughout the normal period of retinal vascularization. METHODS: Infants ≤ 29 wk GA (n = 122, 14 centers) were randomized and treated with placebo or inositol at 10, 40, or 80 mg/kg/d. Intravenous administration converted to enteral when feedings were established, and continued to the first of 10 wk, 34 wk postmenstrual age (PMA) or discharge. Serum collection employed a sparse sampling population pharmacokinetics design. Inositol urine losses and feeding intakes were measured. Safety was prospectively monitored. RESULTS: At 80 mg/kg/d mean serum levels reached 140 mg/l, similar to Hallman's findings. Levels declined after 2 wk, converging in all groups by 6 wk. Analyses showed a mean volume of distribution 0.657 l/kg, clearance 0.058 l/kg/h, and half-life 7.90 h. Adverse events and comorbidities were fewer in the inositol groups, but not significantly so. CONCLUSION: Multiple dose inositol at 80 mg/kg/d was not associated with increased adverse events, achieves previously effective serum levels, and is appropriate for investigation in a phase III trial