130 research outputs found
Développement d’une approche de planification systématique de la conservation des milieux humides et de la sauvagine : étude de cas en Abitibi-Témiscamingue
Une croyance populaire présume que les ressources monétaires investies en protection de l’environnement pénalisent globalement le bien-être d’une société en la privant des bénéfices économiques retirés par l’extraction de ressources. Pourtant la dégradation des écosystèmes négligent les gains communs à long terme procurés par les services écologiques. Il est impératif d’assurer la protection du capital naturel d’un territoire afin d’en conserver les bénéfices à long terme. Par exemple, la conservation des milieux humides permet d’assurer la pérennité de plusieurs espèces de sauvagine chassées. Nous avons développé une approche de planification systématique de la conservation (systematic conservation planning) pour les milieux humides (MH) et la sauvagine (SAU), adaptée à une échelle régionale, dans un contexte où la principale contrainte à la conservation est liée à l’activité minière. Les objectifs étaient d’évaluer 1) la complémentarité de différentes approches de réseaux de conservation de la diversité de milieux humides et de sauvagine; 2) les conséquences du développement de l'activité minière dans l’atteinte de cibles de protection pour les milieux humides et la sauvagine.
Nous avons réalisé une cartographie de dix-huit types de milieux humides et modélisé l’abondance potentielle de huit espèces de sauvagine, à une échelle fine de 4 km2 dans la région administrative de l’Abitibi-Témiscamingue, un territoire boréal de 65 000 km2 au Québec, Canada. En utilisant l’outil Marxan permettant la prise en compte d’objectifs multiples de conservation, nous avons comparé trois types d’approches de construction de réseaux de conservation (MH, SAU, MH+SAU). Les cibles de protection variaient entre 10% à 70% de représentativité de la superficie de milieux humides et d’abondance de sauvagine, tout en calibrant la pression minière (présente sur 42% du territoire d’étude). Nos résultats suggèrent que les réseaux de conservation individuels visant uniquement la protection du capital naturel (MH) ou des bénéfices (SAU) ne protègent pas une aussi grande quantité de milieux humides et de sauvagine qu’une approche combinée (MH+SAU). De plus, l’approche combinée nécessite en moyenne une superficie de territoire moindre de 32% pour atteindre les mêmes cibles de protection, par rapport à la planification de deux réseaux individuels construits en parallèle. L’approche combinée permettait d’encaisser le tiers de l’activité minière potentielle sans avoir d’impact dans l’atteinte de toutes les cibles de conservation. Toutefois, malgré l’ajout de la contrainte minière à niveau de pression maximal, il était toujours possible de protéger jusqu’à 20% des milieux humides et de la sauvagine de l’aire d’étude
Toll-Like Receptor 2-Independent Host Innate Immune Response against an Epidemic Strain of Streptococcus suis That Causes a Toxic Shock-Like Syndrome in Humans
Streptococcus suis is an emerging zoonotic agent causing meningitis and septicemia. Outbreaks in humans in China with atypical cases of streptococcal toxic shock-like syndrome have been described to be caused by a clonal epidemic S. suis strain characterized as sequence type (ST) 7 by multilocus sequence typing, different from the classical ST1 usually isolated in Europe. Previous in vitro studies showed that Toll-like receptor (TLR) 2 plays a major role in S. suis ST1 interactions with host cells. In the present study, the in vivo role of TLR2 in systemic infections caused by S. suis ST1 or ST7 strains using TLR2 deficient (TLR2−/−) mice was evaluated. TLR2-mediated recognition significantly contributes to the acute disease caused by the highly virulent S. suis ST1 strain, since the TLR2−/− mice remained unaffected when compared to wild type (WT) mice. The lack of mortality could not be associated with a lower bacterial burden; however, a significant decrease in the induction of pro-inflammatory mediators, as evaluated by microarray, real-time PCR and protein assays, was observed. On the other hand, TLR2−/− mice infected with the epidemic ST7 strain presented no significant differences regarding survival and expression of pro-inflammatory mediators when compared to the WT mice. Together, these results show a TLR2-independent host innate immune response to S. suis that depends on the strain.Fil: Lachance, Claude. University Of Montreal. Faculty of Veterinary Medicine; Canadá;Fil: Segura, Mariela. University Of Montreal. Faculty of Veterinary Medicine; Canadá;Fil: Pereyra Gerber, Federico Pehuén. Universidad de Buenos Aires. Facultad de Medicina. Departamento de MicrobiologÃa; Argentina; University Of Montreal. Faculty of Veterinary Medicine; Canadá; Consejo Nacional de Investigaciones CientÃficas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Fil: Xu, Jianguo. Chinese Center for Disease Control and Prevention. National Institute for Communicable Disease Control and Prevention. State Key Laboratory for Infectious Disease Prevention and Control; China;Fil: Gottschalk, Marcelo. University Of Montreal. Faculty of Veterinary Medicine; Canadá
Transcriptional analysis of PRRSV-infected porcine dendritic cell response to Streptococcus suis infection reveals up-regulation of inflammatory-related genes expression
The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important
swine pathogens and often serves as an entry door for other viral or bacterial pathogens,
of which Streptococcus suis is one of the most common. Pre-infection with PRRSV
leads to exacerbated disease caused by S. suis infection. Very few studies have assessed
the immunological mechanisms underlying this higher susceptibility. Since antigen presenting
cells play a major role in the initiation of the immune response, the in vitro transcriptional
response of bone marrow-derived dendritic cells (BMDCs) and monocytes in the context of
PRRSV and S. suis co-infection was investigated. BMDCs were found to be more permissive
than monocytes to PRRSV infection; S. suis phagocytosis by PRRSV-infected BMDCs
was found to be impaired, whereas no effect was found on bacterial intracellular survival.
Transcription profile analysis, with a major focus on inflammatory genes, following S. suis
infection, with and without pre-infection with PRRSV, was then performed. While PRRSV
pre-infection had little effect on monocytes response to S. suis infection, a significant
expression of several pro-inflammatory molecules was observed in BMDCs pre-infected
with PRRSV after a subsequent infection with S. suis. While an additive effect could be
observed for CCL4, CCL14, CCL20, and IL-15, a distinct synergistic up-regulatory effect
was observed for IL-6, CCL5 and TNF-α after co-infection. This increased pro-inflammatory
response by DCs could participate in the exacerbation of the disease observed during
PRRSV and S. suis co-infection
Impact of anesthesia on echocardiographic evaluation of systolic and diastolic function in rats
Background : Echocardiography is used on rats but general anesthesia is usually necessary to be able to obtain a good quality echocardiogram. Each type of anesthetic agent has specific impacts on hemodynamics and, therefore, may affect differentially the echocardiographic measurements.
Objectives : We sought to compare the echocardiograms of normal rats and rats with chronic aortic regurgitation under anesthesia using ketamine-xylazine or isoflurane.
Methods : Animals underwent an echocardiogram with both drugs sequentially. Echocardiographic measurements were compared.
Results : Mitral diastolic Doppler measurements (early diastolic filling wave [E] and late atrial diastolic filling wave [A] velocities) were significantly affected by the type of anesthesia in the normal group but not left ventricular dimensions or ejection fraction. Left ventricular dimensions were affected by the type of anesthesia in the aortic regurgitation group and diastolic Doppler flow.
Conclusion : The anesthetic agent has significant specific impacts on many echocardiographic measurements. Investigators working with rat models should be aware of those potential effects
Fructose-fed rats and eccentric LVH
The development of left ventricular (LV) hypertrophy (LVH) can be affected by diet manipulation. Concentric LVH resulting from pressure overload can be worsened by feeding rats with a high-fructose diet. Eccentric LVH is a different type of hypertrophy and is associated with volume overload (VO) diseases. The impact of an abnormal diet on the development of eccentric LVH and on ventricular function in chronic VO is unknown. This study therefore examined the effects of a fructose-rich diet on LV eccentric hypertrophy, ventricular function, and myocardial metabolic enzymes in rats with chronic VO caused by severe aortic valve regurgitation (AR). Wistar rats were divided in four groups: sham-operated on control diet (SC; n = 13) or fructose-rich diet (SF; n = 13) and severe aortic regurgitation fed with the same diets [aortic regurgitation on control diet (ARC), n = 16, and aortic regurgitation on fructose-rich diet (ARF), n = 13]. Fructose-rich diet was started 1 wk before surgery, and the animals were euthanized 9 wk later. SF and ARF had high circulating triglycerides. ARC and ARF developed significant LV eccentric hypertrophy after 8 wk as expected. However, ARF developed more LVH than ARC. LV ejection fraction was slightly lower in the ARF compared with ARC. The increased LVH and decreased ejection fraction could not be explained by differences in hemodynamic load. SF, ARC, and ARF had lower phosphorylation levels of the AMP kinase compared with SC. A fructose-rich diet worsened LV eccentric hypertrophy and decreased LV function in a model of chronic VO caused by AR in rats. Normal animals fed the same diet did not develop these abnormalities. Hypertriglyceridemia may play a central role in this phenomenon as well as AMP kinase activity
Gender differences in left ventricular remodeling in chronic severe aortic valve regurgitation in rats.
BACKGROUND AND AIM OF THE STUDY:
Aortic valve regurgitation (AR) can result in heart failure from chronic overloading of the left ventricle. As little is known of gender-specific responses of the left ventricle to this condition, the study aim was to compare left ventricular (LV) remodeling in male and female rats with severe AR. In order to assess the impact of estrogens on LV remodeling in AR, the effect of ovariectomy (OVX) was also evaluated.
METHODS:
AR was created in adult Wistar rats (females (control or OVX) and males). Animals were followed for 26 weeks and compared to sham-operated groups. Heart function was evaluated in vivo using echocardiography, and the hearts were subsequently harvested for tissue analysis.
RESULTS:
The LV ejection fraction was decreased similarly in both sexes. Despite similar echocardiographic AR severity, females had higher indexed cardiac output and the largest increase in LV weight, cardiomyocyte hypertrophy and eccentric remodeling. No differences were observed between control and OVX females. Ovariectomy had no significant impact on any of the parameters monitored.
CONCLUSION:
Female rats developed more LV remodeling in response to chronic AR than males. AR appears to impose a greater LV workload on females due to their smaller body and heart size. Hormonal status did not have any impact on LV remodeling in this experimental model
Vegetation dynamics on an abandoned vacuum-mined peatland: 5 years of monitoring.
Abstract We studied from 1998 to 2002 the fine-scale vegetation dynamics of a poorly regenerated vacuum-mined bog located in southern Quebec. We selected mined sites that have been abandoned for 14 years and monitored the vascular and non-vascular plants, and some hydrological characteristics. We focussed our study on the monitoring of cotton-grass (Eriophorum vaginatum L.) tussocks. Major changes in the plant cover were observed during the five-year period, such as a decrease (26-31%) in the number of cotton-grass tussocks and an increase in the ericaceous shrub cover. The water table level (lower than 40 cm below the soil surface) and frost heaving appear to be the main factors explaining the failure of cotton-grass and of other typical bog plant species to colonize abandoned mined surfaces. The ericaceous shrub cover, although increasing, was still sparse even after two decades of abandonment, and it may take several additional decades before a complete shrub cover establishes itself. While the natural revegetation process of this vacuum-mined bog is still dynamic after two decades of abandonment, there is no evidence to suggest that vegetation assemblages will eventually resemble those of undisturbed peatlands
Characterization of porcine dendritic cell response to Streptococcus suis
Streptococcus suis is a major swine pathogen and important zoonotic agent causing mainly septicemia and meningitis. However, the mechanisms involved in host innate and adaptive immune responses toward S. suis as well as the mechanisms used by S. suis to subvert these responses are unknown. Here, and for the first time, the ability of S. suis to interact with bone marrow-derived swine dendritic cells (DCs) was evaluated. In addition, the role of S. suis capsular polysaccharide in modulation of DC functions was also assessed. Well encapsulated S. suis was relatively resistant to phagocytosis, but it increased the relative expression of Toll-like receptors 2 and 6 and triggered the release of several cytokines by DCs, including IL-1β, IL-6, IL-8, IL-12p40 and TNF-α. The capsular polysaccharide was shown to interfere with DC phagocytosis; however, once internalized, S. suis was readily destroyed by DCs independently of the presence of the capsular polysaccharide. Cell wall components were mainly responsible for DC activation, since the capsular polysaccharide-negative mutant induced higher cytokine levels than the wild-type strain. The capsular polysaccharide also interfered with the expression of the co-stimulatory molecules CD80/86 and MHC-II on DCs. To conclude, our results show for the first time that S. suis interacts with swine origin DCs and suggest that these cells might play a role in the development of host innate and adaptive immunity during an infection with S. suis serotype 2
Hypertension and aortic valve regurgitation in rats
Background : Hypertension (HT) and aortic valve regurgitation (AR) often coexist but the specific impacts of AR + HT on the left ventricle (LV) are still unknown. The best treatment strategy for this combination of diseases is also unclear. The objectives of this study were 1) to evaluate LV function, remodeling and 2) to assess the effects of the angiotensin-converting enzyme (ACE) inhibitor captopril (C) in rats with AR ± HT in spontaneously hypertensive rats (SHR).
Methods : Animals were grouped as follows: normotensive (NT) Wistar-Kyoto, NT + AR, hypertensive SHR (HT), and HT + AR receiving or not captopril (150 mg/kg/d). Hearts were evaluated in vivo by echocardiography and harvested for tissue analysis after 6 months of evolution.
Results : The HT + AR rats had the worst LV hypertrophy (LVH), subendocardial fibrosis, and lowest ejection fraction. Captopril normalized BP in HT and HT + AR, but could not prevent LVH in HT + AR as well as it did in isolated HT. The LV ejection fraction remained below normal in HT + AR + captopril compared to HT alone + captopril. Cardiomyocyte hypertrophy remained in HT + AR + captopril but was normalized in HT + captopril. Subendocardial fibrosis was reduced by captopril in HT + AR.
Conclusions : The AR + HT rats had the most severe myocardial abnormalities. High dose captopril was effective to slow LVH and preserve normal LV ejection fraction in isolated HT or AR, but was less effective when both pathologies were combined. Prohypertrophic stimuli clearly remain active in HT + AR despite ACE inhibition. These results suggest that a very aggressive medical treatment strategy may be required to optimize LV protection when AR and HT co-exist
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