1,516 research outputs found
A Regularized Graph Layout Framework for Dynamic Network Visualization
Many real-world networks, including social and information networks, are
dynamic structures that evolve over time. Such dynamic networks are typically
visualized using a sequence of static graph layouts. In addition to providing a
visual representation of the network structure at each time step, the sequence
should preserve the mental map between layouts of consecutive time steps to
allow a human to interpret the temporal evolution of the network. In this
paper, we propose a framework for dynamic network visualization in the on-line
setting where only present and past graph snapshots are available to create the
present layout. The proposed framework creates regularized graph layouts by
augmenting the cost function of a static graph layout algorithm with a grouping
penalty, which discourages nodes from deviating too far from other nodes
belonging to the same group, and a temporal penalty, which discourages large
node movements between consecutive time steps. The penalties increase the
stability of the layout sequence, thus preserving the mental map. We introduce
two dynamic layout algorithms within the proposed framework, namely dynamic
multidimensional scaling (DMDS) and dynamic graph Laplacian layout (DGLL). We
apply these algorithms on several data sets to illustrate the importance of
both grouping and temporal regularization for producing interpretable
visualizations of dynamic networks.Comment: To appear in Data Mining and Knowledge Discovery, supporting material
(animations and MATLAB toolbox) available at
http://tbayes.eecs.umich.edu/xukevin/visualization_dmkd_201
Neural development features: Spatio-temporal development of the Caenorhabditis elegans neuronal network
The nematode Caenorhabditis elegans, with information on neural connectivity,
three-dimensional position and cell linage provides a unique system for
understanding the development of neural networks. Although C. elegans has been
widely studied in the past, we present the first statistical study from a
developmental perspective, with findings that raise interesting suggestions on
the establishment of long-distance connections and network hubs. Here, we
analyze the neuro-development for temporal and spatial features, using birth
times of neurons and their three-dimensional positions. Comparisons of growth
in C. elegans with random spatial network growth highlight two findings
relevant to neural network development. First, most neurons which are linked by
long-distance connections are born around the same time and early on,
suggesting the possibility of early contact or interaction between connected
neurons during development. Second, early-born neurons are more highly
connected (tendency to form hubs) than later born neurons. This indicates that
the longer time frame available to them might underlie high connectivity. Both
outcomes are not observed for random connection formation. The study finds that
around one-third of electrically coupled long-range connections are late
forming, raising the question of what mechanisms are involved in ensuring their
accuracy, particularly in light of the extremely invariant connectivity
observed in C. elegans. In conclusion, the sequence of neural network
development highlights the possibility of early contact or interaction in
securing long-distance and high-degree connectivity
Appropriate use criteria for endotracheal suction interventions in mechanically ventilated children : the RAND/UCLA development process
Objectives:
Endotracheal suction is an invasive airway clearance technique used in mechanically ventilated children. This article outlines the methods used to develop appropriate use criteria for endotracheal suction interventions in mechanically ventilated paediatric patients.
Methods:
The RAND Corporation and University of California, Los Angeles Appropriateness Method was used to develop paediatric appropriate use criteria. This included the following sequential phases of defining scope and key terms, a literature review and synthesis, expert multidisciplinary panel selection, case scenario development, and appropriateness ratings by an interdisciplinary expert panel over two rounds. The panel comprised experts in the fields of paediatric and neonatal intensive care, respiratory medicine, infectious diseases, critical care nursing, implementation science, retrieval medicine, and education. Case scenarios were developed iteratively by interdisciplinary experts and derived from common applications or anticipated intervention uses, as well as from current clinical practice guidelines and results of studies examining interventions efficacy and safety. Scenarios were rated on a scale of 1 (harm outweighs benefit) to 9 (benefit outweighs harm), to define appropriate use (median: 7 to 9), uncertain use (median: 4 to 6), and inappropriate use (median: 1 to 3) of endotracheal suction interventions. Scenarios were than classified as a level of appropriateness.
Conclusions:
The RAND Corporation/University of California, Los Angeles Appropriateness Method provides a thorough and transparent method to inform development of the first appropriate use criteria for endotracheal suction interventions in paediatric patients
An alginate-layer technique for culture of Brassica oleracea L. protoplasts
Ten accessions belonging to the Brassica oleracea subspecies alba and rubra, and to B. oleracea var. sabauda were used in this study. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants. The influence of selected factors on the yield, viability, and mitotic activity of protoplasts immobilized in calcium alginate layers was investigated. The efficiency of protoplast isolation from hypocotyls was lower (0.7 ± 0.1 × 106 ml−1) than for protoplasts isolated from leaf mesophyll tissue (2 ± 0.1 × 106 ml−1). High (70–90%) viabilities of immobilized protoplasts were recorded, independent of the explant sources. The highest proportion of protoplasts undergoing divisions was noted for cv. Reball F1, both from mesophyll (29.8 ± 2.2%) and hypocotyl (17.5 ± 0.3%) tissues. Developed colonies of callus tissue were subjected to regeneration and as a result plants from six accessions were obtained
Prompt atmospheric neutrino fluxes: perturbative QCD models and nuclear effects
We evaluate the prompt atmospheric neutrino flux at high energies using three
different frameworks for calculating the heavy quark production cross section
in QCD: NLO perturbative QCD, factorization including low-
resummation, and the dipole model including parton saturation. We use QCD
parameters, the value for the charm quark mass and the range for the
factorization and renormalization scales that provide the best description of
the total charm cross section measured at fixed target experiments, at RHIC and
at LHC. Using these parameters we calculate differential cross sections for
charm and bottom production and compare with the latest data on forward charm
meson production from LHCb at TeV and at TeV, finding good agreement
with the data. In addition, we investigate the role of nuclear shadowing by
including nuclear parton distribution functions (PDF) for the target air
nucleus using two different nuclear PDF schemes. Depending on the scheme used,
we find the reduction of the flux due to nuclear effects varies from to
at the highest energies. Finally, we compare our results with the
IceCube limit on the prompt neutrino flux, which is already providing valuable
information about some of the QCD models.Comment: 61 pages, 25 figures, 11 table
Higgs mass and vacuum stability in the Standard Model at NNLO
We present the first complete next-to-next-to-leading order analysis of the
Standard Model Higgs potential. We computed the two-loop QCD and Yukawa
corrections to the relation between the Higgs quartic coupling (lambda) and the
Higgs mass (Mh), reducing the theoretical uncertainty in the determination of
the critical value of Mh for vacuum stability to 1 GeV. While lambda at the
Planck scale is remarkably close to zero, absolute stability of the Higgs
potential is excluded at 98% C.L. for Mh < 126 GeV. Possible consequences of
the near vanishing of lambda at the Planck scale, including speculations about
the role of the Higgs field during inflation, are discussed.Comment: 35 pages, 8 figures. Final published version, misprints fixed,
figures update
A cross-national study on the antecedents of work–life balance from the fit and balance perspective
Drawing on the perceived work–family fit and balance perspective, this study investigates demands and resources as antecedents of work–life balance (WLB) across four countries (New Zealand, France, Italy and Spain), so as to provide empirical cross-national evidence. Using structural equation modelling analysis on a sample of 870 full time employees, we found that work demands, hours worked and family demands were negatively related to WLB, while job autonomy and supervisor support were positively related to WLB. We also found evidence that resources (job autonomy and supervisor support) moderated the relationships between demands and work–life balance, with high resources consistently buffering any detrimental influence of demands on WLB. Furthermore, our study identified additional predictors of WLB that were unique to some national contexts. For example, in France and Italy, overtime hours worked were negatively associated with WLB, while parental status was positively associated with WLB. Overall, the implications for theory and practice are discussed.Peer ReviewedPostprint (author's final draft
Sustained Delivery of Activated Rho GTPases and BDNF Promotes Axon Growth in CSPG-Rich Regions Following Spinal Cord Injury
Background: Spinal cord injury (SCI) often results in permanent functional loss. This physical trauma leads to secondary events, such as the deposition of inhibitory chondroitin sulfate proteoglycan (CSPG) within astroglial scar tissue at the lesion. Methodology/Principal Findings: We examined whether local delivery of constitutively active (CA) Rho GTPases, Cdc42 and Rac1 to the lesion site alleviated CSPG-mediated inhibition of regenerating axons. A dorsal over-hemisection lesion was created in the rat spinal cord and the resulting cavity was conformally filled with an in situ gelling hydrogel combined with lipid microtubes that slowly released constitutively active (CA) Cdc42, Rac1, or Brain-derived neurotrophic factor (BDNF). Treatment with BDNF, CA-Cdc42, or CA-Rac1 reduced the number of GFAP-positive astrocytes, as well as CSPG deposition, at the interface of the implanted hydrogel and host tissue. Neurofilament 160kDa positively stained axons traversed the glial scar extensively, entering the hydrogel-filled cavity in the treatments with BDNF and CA-Rho GTPases. The treated animals had a higher percentage of axons from the corticospinal tract that traversed the CSPG-rich regions located proximal to the lesion site. Conclusion: Local delivery of CA-Cdc42, CA-Rac1, and BDNF may have a significant therapeutic role in overcoming CSPGmediate
Molecular characterisation of protist parasites in human-habituated mountain gorillas (Gorilla beringei beringei), humans and livestock, from Bwindi impenetrable National Park, Uganda
Over 60 % of human emerging infectious diseases are zoonotic, and there is growing evidence of the zooanthroponotic transmission of diseases from humans to livestock and wildlife species, with major implications for public health, economics, and conservation. Zooanthroponoses are of relevance to critically endangered species; amongst these is the mountain gorilla (Gorilla beringei beringei) of Uganda. Here, we assess the occurrence of Cryptosporidium, Cyclospora, Giardia, and Entamoeba infecting mountain gorillas in the Bwindi Impenetrable National Park (BINP), Uganda, using molecular methods. We also assess the occurrence of these parasites in humans and livestock species living in overlapping/adjacent geographical regions
Towards the clinical implementation of pharmacogenetics in bipolar disorder.
BackgroundBipolar disorder (BD) is a psychiatric illness defined by pathological alterations between the mood states of mania and depression, causing disability, imposing healthcare costs and elevating the risk of suicide. Although effective treatments for BD exist, variability in outcomes leads to a large number of treatment failures, typically followed by a trial and error process of medication switches that can take years. Pharmacogenetic testing (PGT), by tailoring drug choice to an individual, may personalize and expedite treatment so as to identify more rapidly medications well suited to individual BD patients.DiscussionA number of associations have been made in BD between medication response phenotypes and specific genetic markers. However, to date clinical adoption of PGT has been limited, often citing questions that must be answered before it can be widely utilized. These include: What are the requirements of supporting evidence? How large is a clinically relevant effect? What degree of specificity and sensitivity are required? Does a given marker influence decision making and have clinical utility? In many cases, the answers to these questions remain unknown, and ultimately, the question of whether PGT is valid and useful must be determined empirically. Towards this aim, we have reviewed the literature and selected drug-genotype associations with the strongest evidence for utility in BD.SummaryBased upon these findings, we propose a preliminary panel for use in PGT, and a method by which the results of a PGT panel can be integrated for clinical interpretation. Finally, we argue that based on the sufficiency of accumulated evidence, PGT implementation studies are now warranted. We propose and discuss the design for a randomized clinical trial to test the use of PGT in the treatment of BD
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