Efeitos da frequência de fornecimento do volumoso e concentrado no comportamento ingestivo de vacas e novilhas em confinamento

Este estudo foi conduzido com o objetivo de avaliar a influência da frequência no fornecimento de volumoso e concentrado e/ou da categoria animal sobre o comportamento ingestivo de bovinos. Foram utilizadas 16 novilhas e 16 vacas com idade média inicial de 20 e 66 meses e peso médio inicial de 338 e 432 kg, respectivamente. Os tratamentos foram representados pelas frequências de fornecimento do volumoso e do concentrado: 2 V/C - volumoso e concentrado duas vezes ao dia; 1 V/C - volumoso e concentrado uma vez ao dia; 1 V/2 C - volumoso uma vez ao dia e concentrado duas vezes ao dia; 1 V/3 C - volumoso uma vez ao dia e concentrado três vezes ao dia, ofertadas às categorias vacas e novilhas. A dieta foi composta de 60% de silagem de milho e 40% de concentrado com base na matéria seca (MS). O delineamento experimental foi inteiramente casualizado em arranjo fatorial 4 × 2 (frequências × categorias). Os dados foram submetidos à análise de variância e as médias, comparadas pelo teste t. A interação frequência de fornecimento e categoria animal foi significativa para as características tempo de ruminação e ócio, eficiência de ruminação da matéria seca e da fibra detergente neutro, número de bolos diário e tempo diário de mastigação. Em relação às vacas, as novilhas apresentaram maiores tempos de ócio e mastigação quando receberam a alimentação uma vez ao dia. A frequência de fornecimento da dieta não influenciou o tempo de consumo. As vacas apresentaram maior tempo consumindo alimento e taxa de ingestão em comparação às novilhas (4,38 vs 4,09 horas e 2,91 vs 2,35 kg de MS/hora de consumo, respectivamente). O aumento na frequência da alimentação estimula os animais a maior ingestão de alimento no momento do fornecimento.The present study was conducted with the objective of evaluating the influence of frequency of roughage and supplement supply and/or animal category on the ingestive behavior of cattle. Sixteen heifers and sixteen cows, with average initial age of 20 and 66 months and average initial weight of 338 and 432 kg, respectively, were used. Treatments were represented by the frequencies of roughage and concentrate supply: 2 R/C - roughage and concentrate two times per day; 1 R/C - roughage and concentrate one time per day; 1 R/2C - roughage one time per day and concentrate two times per day; 1 R/3 C - roughage one time per day and concentrate three times per day, offered to cows and heifers. Diet was composed of 60% maize silage and 40% concentrate, on a dry matter basis (DM). The complete randomized experimental design was used, with a 4 × 2 factorial arrangement (frequencies × category). The data were submitted to variance analysis and averages were compared by "t" test. The interaction between supply frequencies and animal category was significant for rumination and idle times, efficiencies of dry matter rumination and of neutral detergent fiber rumination, number of ruminated bolus and daily time of chew. Heifers showed longer idle periods and daily time of chew when compared with cows that received food once in a day. Duration of feed intake did not vary with frequencies. Cows showed longer period of feed intake and ingestive rate in relation to heifers (4.38 vs. 4.09 hours and 2.91 vs. 2.35 kg of DM/hour of intake, respectively). The increase in food frequency stimulated animals to intensify feed intake at the moment of supply

The GHEP–EMPOP collaboration on mtDNA population data—A new resource for forensic casework

AbstractMitochondrial DNA (mtDNA) population data for forensic purposes are still scarce for some populations, which may limit the evaluation of forensic evidence especially when the rarity of a haplotype needs to be determined in a database search. In order to improve the collection of mtDNA lineages from the Iberian and South American subcontinents, we here report the results of a collaborative study involving nine laboratories from the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) and EMPOP. The individual laboratories contributed population data that were generated throughout the past 10 years, but in the majority of cases have not been made available to the scientific community. A total of 1019 haplotypes from Iberia (Basque Country, 2 general Spanish populations, 2 North and 1 Central Portugal populations), and Latin America (3 populations from São Paulo) were collected, reviewed and harmonized according to defined EMPOP criteria. The majority of data ambiguities that were found during the reviewing process (41 in total) were transcription errors confirming that the documentation process is still the most error-prone stage in reporting mtDNA population data, especially when performed manually. This GHEP–EMPOP collaboration has significantly improved the quality of the individual mtDNA datasets and adds mtDNA population data as valuable resource to the EMPOP database (www.empop.org)