Assessing the mechanical stresses of dynamic cables for floating offshore wind applications

This is the final version. Available from IOP Publishing via the DOI in this record.WindEurope conference 2018 within the Global Wind Summit 25–28 September 2018, Hamburg, GermanyOffshore wind farms are progressing further offshore and into deeper waters, presenting the need for new substructures, including floating offshore wind turbines. These floating turbines will require dynamic cables to run through the water column, exposing them to the dynamic loadings of the marine environment. This paper presents a tool which models the stresses across a dynamic cable cross section’s insulation layers when attached to a floating wind platform. Differing wave, wind and current flow conditions are applied and their impact on the stress distributions of the dynamic cable’s insulation layers are presented. Finally from these stress histories, accumulated fatigue damage of the insulation is calculated and presented. The outcome of this can be used to estimate fatigue damage of a cable components cross section at any point along the cable length, and aid in cable installation configuration decisions.The authors would like to thank the Energy Technology Institute and the Research Council Energy Programme for funding this research as part of the IDCORE programme (grant EP/J500847), and the Offshore Renewable Energy Catapult for providing technical assistance and equipment. A special thanks to Orcina for providing OrcaFlex software and to JDR cables for allowing presentation of cable data

Ground state fidelity in bond-alternative Ising chains with Dzyaloshinskii-Moriya interactions

A systematic analysis is performed for quantum phase transitions in a bond-alternative one-dimensional Ising model with a Dzyaloshinskii-Moriya (DM) interaction by using the fidelity of ground state wave functions based on the infinite matrix product states algorithm. For an antiferromagnetic phase, the fidelity per lattice site exhibits a bifurcation, which shows spontaneous symmetry breaking in the system. A critical DM interaction is inversely proportional to an alternating exchange coupling strength for a quantum phase transition. Further, a finite-entanglement scaling of von Neumann entropy with respect to truncation dimensions gives a central charge c = 0.5 at the critical point.Comment: 6 pages, 4 figure

Granzyme B (GraB) Autonomously Crosses the Cell Membrane and Perforin Initiates Apoptosis and GraB Nuclear Localization

Granzyme B (GraB) induces apoptosis in the presence of perforin. Perforin polymerizes in the cell membrane to form a nonspecific ion pore, but it is not known where GraB acts to initiate the events that ultimately lead to apoptosis. It has been hypothesized that GraB enters the target cell through a perforin channel and then initiates apoptosis by cleaving and activating members of the ICE/Ced-3 family of cell death proteases. To determine if GraB can enter the cell, we treated YAC-1 or HeLa cells with FITC-labeled GraB and measured intracellular fluorescence with a high sensitivity CCD camera and image analyzer. GraB was internalized and found diffusely dispersed in the cell cytoplasm within 10 min. Uptake was inhibited at low temperature (4°C) and by pretreatment with metabolic inhibitors, NaF and DNP, or cytochalasin B, a drug that both blocks microfilament formation, and FITC-GraB remained on the cell membrane localized in patches. With the simultaneous addition of perforin and FITC-GraB, no significant increase in cytoplasmic fluorescence was observed over that found in cells treated only with FITC-GraB. However, FITC-GraB was now detected in the nucleus of apoptotic cells labeling apoptotic bodies and localized areas within and along the nuclear membrane. The ability of GraB to enter cells in the absence of perforin was reexamined using anti-GraB antibody immunogold staining of ultrathin cryosections of cells incubated with GraB. Within 15 min, gold particles were detected both on the plasma membrane and in the cytoplasm of cells with some gold staining adjacent to the nuclear envelope but not in the nucleus. Cells internalizing GraB in the absence of perforin appeared morphologically normal by Hoechst staining and electron microscopy. GraB directly microinjected into the cytoplasm of B16 melanoma cells induced transient plasma membrane blebbing and nuclear coarsening but the cells did not become frankly apoptotic unless perforin was added. We conclude that GraB can enter cells autonomously but that perforin initiates the apoptotic process and the entry of GraB into the nucleus

Transcriptionally active HERV-H retrotransposons demarcate topologically associating domains in human pluripotent stem cells.

Chromatin architecture has been implicated in cell type-specific gene regulatory programs, yet how chromatin remodels during development remains to be fully elucidated. Here, by interrogating chromatin reorganization during human pluripotent stem cell (hPSC) differentiation, we discover a role for the primate-specific endogenous retrotransposon human endogenous retrovirus subfamily H (HERV-H) in creating topologically associating domains (TADs) in hPSCs. Deleting these HERV-H elements eliminates their corresponding TAD boundaries and reduces the transcription of upstream genes, while de novo insertion of HERV-H elements can introduce new TAD boundaries. The ability of HERV-H to create TAD boundaries depends on high transcription, as transcriptional repression of HERV-H elements prevents the formation of boundaries. This ability is not limited to hPSCs, as these actively transcribed HERV-H elements and their corresponding TAD boundaries also appear in pluripotent stem cells from other hominids but not in more distantly related species lacking HERV-H elements. Overall, our results provide direct evidence for retrotransposons in actively shaping cell type- and species-specific chromatin architecture

A Directional Entropic Force Approach to Assemble Anisotropic Nanoparticles into Superlattices

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/102048/1/13980_ftp.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102048/2/anie_201306009_sm_miscellaneous_information.pd

Mapping interactions with the chaperone network reveals factors that protect against tau aggregation.

A network of molecular chaperones is known to bind proteins ('clients') and balance their folding, function and turnover. However, it is often unclear which chaperones are critical for selective recognition of individual clients. It is also not clear why these key chaperones might fail in protein-aggregation diseases. Here, we utilized human microtubule-associated protein tau (MAPT or tau) as a model client to survey interactions between ~30 purified chaperones and ~20 disease-associated tau variants (~600 combinations). From this large-scale analysis, we identified human DnaJA2 as an unexpected, but potent, inhibitor of tau aggregation. DnaJA2 levels were correlated with tau pathology in human brains, supporting the idea that it is an important regulator of tau homeostasis. Of note, we found that some disease-associated tau variants were relatively immune to interactions with chaperones, suggesting a model in which avoiding physical recognition by chaperone networks may contribute to disease

A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia

In vitro gene chemical synthesis is a powerful tool to improve the expression of gene in heterologous system. In this study, a two-step gene synthesis strategy that combines an assembly PCR and an overlap extension PCR (AOE) was developed. In this strategy, the chemically synthesized oligonucleotides were assembled into several 200–500 bp fragments with 20–25 bp overlap at each end by assembly PCR, and then an overlap extension PCR was conducted to assemble all these fragments into a full length DNA sequence. Using this method, we de novo designed and optimized the codon of Rhizopus oryzae lipase gene ROL (810 bp) and Aspergillus niger phytase gene phyA (1404 bp). Compared with the original ROL gene and phyA gene, the codon-optimized genes expressed at a significantly higher level in yeasts after methanol induction. We believe this AOE method to be of special interest as it is simple, accurate and has no limitation with respect to the size of the gene to be synthesized. Combined with de novo design, this method allows the rapid synthesis of a gene optimized for expression in the system of choice and production of sufficient biological material for molecular characterization and biotechnological application

Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in E scherichia coli

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/96334/1/mmi12105.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/96334/2/mmi12105-sup-0001-si.pd