A generic greedy algorithm, partially-ordered graphs and NP-completeness.

Let π be any fixed polynomial-time testable, non-trivial, hereditary property of graphs. Suppose that the vertices of a graph G are not necessarily linearly ordered but partially ordered, where we think of this partial order as a collection of (possibly exponentially many) linear orders in the natural way. We prove that the problem of deciding whether a lexicographically first maximal subgraph of G satisfying π, with respect to one of these linear orders, contains a specified vertex is NP-complete

Interaction of the Streptomyces Wbl protein WhiD with the principal sigma factor σHrdB depends on the WhiD [4Fe-4S] cluster

The bacterial protein WhiD belongs to the Wbl family of iron–sulfur [Fe-S] proteins present only in the actinomycetes. In Streptomyces coelicolor, it is required for the late stages of sporulation, but precisely how it functions is unknown. Here, we report results from in vitro and in vivo experiments with WhiD from Streptomyces venezuelae (SvWhiD), which differs from S. coelicolor WhiD (ScWhiD) only at the C terminus. We observed that, like ScWhiD and other Wbl proteins, SvWhiD binds a [4Fe-4S] cluster that is moderately sensitive to O2 and highly sensitive to nitric oxide (NO). However, although all previous studies have reported that Wbl proteins are monomers, we found that SvWhiD exists in a monomer–dimer equilibrium associated with its unusual C-terminal extension. Several Wbl proteins of Mycobacterium tuberculosis are known to interact with its principal sigma factor SigA. Using bacterial two-hybrid, gel filtration, and MS analyses, we demonstrate that SvWhiD interacts with domain 4 of the principal sigma factor of Streptomyces, σHrdB (σHrdB4). Using MS, we determined the dissociation constant (Kd) for the SvWhiD–σHrdB4 complex as ~0.7 μM, consistent with a relatively tight binding interaction. We found that complex formation was cluster dependent and that a reaction with NO, which was complete at 8–10 NO molecules per cluster, resulted in dissociation into the separate proteins. The SvWhiD [4Fe-4S] cluster was significantly less sensitive to reaction with O2 and NO when SvWhiD was bound to σHrdB4, consistent with protection of the cluster in the complex

Characterisation of friction and lubrication regimes in premium tubular connections

A friction test rig has been developed to carry out repeated sliding friction tests for premium tubular connections. The test rig enables accurate measurement of friction in various contact regimes which are relevant to the threaded connections between tubular components. Higher load tests can simulate the contact in metal-to-metal seals under very high contact pressures by using perpendicular pin-on-pin tests. The contact in the thread loading flank under intermediate pressures can be simulated by using larger radius coupon-on-coupon tests. The measured coefficient of friction is well correlated with a lubrication parameter combining lubricant film thickness and initial surface roughness. © 2012 Elsevier Ltd. All rights reserved

Bulletin No. 224 - Muck Soil Investigations: Progress Report, Sanpete County Experimental Farm 1927-30, Inclusive

The estimated area of muck or peat soil in Utah is approximately 21,000 acres. The muck occurs in many valleys of Utah. The largest areas are in the Sanpete and Utah Lake Valleys. The area in the former is estimated at 6500 acres and the Utah Valley area at 9000 acres; the other areas, all more or less significant in size, are scattered throughout the state. Investigations reported have been confined entirely to the Sanpete area which is located near the south and bottom end of the valley and is west of the towns of Chester, Ephraim, and Manti. During part of the year the lands involved are subject to overflow by the San Pitch River. Water covers most of the area early in the winter and during the spring months high water keeps it submerged until June. Before the neighboring communities were settled by white people, the area was probably submerged the greater part of the year. As the section developed, most of the water from the San Pitch River was diverted for irrigation purposes, resulting in an annual decrease of water which reached the swamp

The Impact of Adult Attention Deficit Hyperactivity Disorder Symptoms on Maternal Parenting Behaviors

The purpose of this qualitative research project is to examine the impact of maternal ADHD symptoms on managing the responsibilities related to parent-ing. The primary researcher conducted three interviews with mothers who exhibit ADHD symptoms in order to gain insight into their perceived difficulties related to the disorder. Analysis of the data indicates these mothers have diffi-culty completing tasks, are disorganized, and are easily overwhelmed with the responsibilities of parenting resulting in difficulty managing their own behavior and being consistent in disciplining their children. Due to these areas of defi-ciency, it is suggested the presence of maternal ADHD symptoms results in less than optimal parenting behaviors, which could result in negative out-comes for children

Generation of 34S-substituted protein-bound [4Fe-4S] clusters using 34S-L-cysteine

The ability to specifically label the sulphide ions of protein-bound iron–sulphur (FeS) clusters with 34S isotope greatly facilitates structure–function studies. In particular, it provides insight when using either spectroscopic techniques that probe cluster-associated vibrations, or non-denaturing mass spectrometry, where the ∼+2 Da average increase per sulphide enables unambiguous assignment of the FeS cluster and, where relevant, its conversion/degradation products. Here, we employ a thermostable homologue of the O-acetyl-L-serine sulfhydrylase CysK to generate 34S-substituted L-cysteine and subsequently use it as a substrate for the L-cysteine desulfurase NifS to gradually supply 34S2− for in vitro FeS cluster assembly in an otherwise standard cluster reconstitution protocol

In-orbit Vignetting Calibrations of XMM-Newton Telescopes

We describe measurements of the mirror vignetting in the XMM-Newton Observatory made in-orbit, using observations of SNR G21.5-09 and SNR 3C58 with the EPIC imaging cameras. The instrument features that complicate these measurements are briefly described. We show the spatial and energy dependences of measured vignetting, outlining assumptions made in deriving the eventual agreement between simulation and measurement. Alternate methods to confirm these are described, including an assessment of source elongation with off-axis angle, the surface brightness distribution of the diffuse X-ray background, and the consistency of Coma cluster emission at different position angles. A synthesis of these measurements leads to a change in the XMM calibration data base, for the optical axis of two of the three telescopes, by in excess of 1 arcminute. This has a small but measureable effect on the assumed spectral responses of the cameras for on-axis targets.Comment: Accepted by Experimental Astronomy. 26 pages, 18 figure

Remarks on Hubble Induced Mass from Fermion Kinetic Term

We evaluate the effective mass of a scalar field which interacts with visible sector via Planck-suppressed coupling in supergravity framework. We focus on the radiation-dominated (RD) era after inflation and the contribution from a fermionic field in the thermal bath. We find that, in RD era, the fermion kinetic term gives the effective mass of the order of Hubble scale to the scalar field.Comment: 8 pages, v2: minor corrections made, published in PL

Super Resolution of Remote Sensing Images Using Edge-Directed Radial Basis Functions

Edge-Directed Radial Basis Functions (EDRBF) are used to compute super resolution(SR) image from a given set of low resolution (LR) images differing in subpixel shifts. The algorithm is tested on remote sensing images and compared for accuracy with other well-known algorithms such as Iterative Back Projection (IBP), Maximum Likelihood (ML) algorithm, interpolation of scattered points using Nearest Neighbor (NN) and Inversed Distance Weighted (IDW) interpolation, and Radial Basis Functin(RBF) . The accuracy of SR depends on various factors besides the algorithm (i) number of subpixel shifted LR images (ii) accuracy with which the LR shifts are estimated by registration algorithms (iii) and the targeted spatial resolution of SR. In our studies, the accuracy of EDRBF is compared with other algorithms keeping these factors constant. The algorithm has two steps: i) registration of low resolution images and (ii) estimating the pixels in High Resolution (HR) grid using EDRBF. Experiments are conducted by simulating LR images from a input HR image with different sub-pixel shifts. The reconstructed SR image is compared with input HR image to measure the accuracy of the algorithm using sum of squared errors (SSE). The algorithm has outperformed all of the algorithms mentioned above. The algorithm is robust and is not overly sensitive to the registration inaccuracies

Late Ordovician to early Silurian acritarchs from the Qusaiba-1 shallow core hole, central Saudi Arabia

Well-preserved acritarchs are documented from Upper Ordovician and lower Silurian sections in the Qusaiba-1 shallow core hole of central Saudi Arabia. Sixty-nine genera comprising 68 named species and 62 forms under open nomenclature were recorded from forty core samples. At the base of the Upper Ordovician and lower Silurian succession in Qusaiba-1 is the Quwarah Member of the Qasim Formation. This is overlain by glacio-marine deposits of the Sarah Formation, which are overlain in turn by the Qusaiba Member of the Qalibah Formation. Four distinct acritarch assemblages are informally numbered 1 to 4 from the base of the core upwards. Assemblage 1 is from the Quwarah Member, and is independently dated by Chitinozoa as being late Katian to early Hirnantian in age (Late Ordovician). The assemblage contains a number of new species, plus species reported from low-latitude Late Ordovician Laurentia and Baltica as well as the Gondwanan margin. Assemblage 2 is from a glacitectonite at the base of the Sarah Formation and is early Hirnantian in age. Assemblage 3, from the Baq'a Shale Member of the Sarah Formation, is also Hirnantian in age and is characterized by a stratigraphically admixed Ordovician palynoflora. Assemblage 4 is restricted to three samples from the Qusaiba Member in the lowermost part of the Qalibah Formation and is dated as Rhuddanian (earliest Silurian). The highest of the three samples that comprise Assemblage 4 is from the same level as a gamma ray peak at 254.8 ft. Reworking of Middle Ordovician forms is evident in Assemblage 3 and is attributed to processes of glacial erosion and resedimentation during glacial melting. Reworked specimens are probably from the Hanadir Member and possibly also the Kahfah Member of the Qasim Formation. The extent of later Ordovician reworking in Assemblage 3, for example reworking from the Quwarah Member, is unclear. However, given that glacial erosion extended to levels below the Quwarah Member, Late Ordovician palynomorphs present in Assemblage 3 might also be reworked. The extent of any reworking in assemblages 1 and 2 is uncertain. There is no evidence for reworking in Assemblage 4. Two new acritarch genera, five new species and one new combination are proposed: Dorsennidium polorum (Miller and Eames, 1982) comb. nov., Falavia magniretifera gen. et sp. nov., Inflatarium trilobatum gen. et sp. nov., Nexosarium mansouri sp. nov., Orthosphaeridium orthogonium sp. nov. and Tunisphaeridium bicaudatum sp. nov. Samples from the same set were used for chitinozoan, scolecodont and miospore studies (this volume). Eurypterid and graptolite remains are also presen