1,007 research outputs found
Neuroprotective potential of isothiocyanates in an in vitro model of neuroinflammation
Isothiocyanates (ITCs), present as glucosinolate precursors in cruciferous vegetables, have shown anti-inflammatory, antioxidant
and anticarcinogenic activities. Here, we compared the effects of three different ITCs on ROS production and on
the expression of matrix metalloproteinase (MMP)-2 and -9, which represent important pathogenetic factors of various
neurological diseases. Primary cultures of rat astrocytes were activated by LPS and simultaneously treated with different
doses of Allyl isothiocyanate (AITC), 2-Phenethyl isothiocyanate (PEITC) and 2-Sulforaphane (SFN). Results showed
that SFN and PEITC were able to counteract ROS production induced by H2O2.
The zymographic analysis of cell culture
supernatants evidenced that PEITC and SFN were the most effective inhibitors of MMP-9, whereas, only SFN significantly
inhibited MMP-2 activity. PCR analysis showed that all the ITCs used significantly inhibited both MMP-2 and MMP-9
expression. The investigation on the mitogen-activated protein kinase (MAPK) signaling pathway demonstrated that ITCs
modulate MMP transcription by inhibition of extracellular-regulated protein kinase (ERK) activity. Results of this study
suggest that ITCs could be promising nutraceutical agents for the prevention and complementary treatment of neurological
diseases associated with MMP involvement
Neuroprotective potential of isothiocyanates in an in vitro model of neuroinflammation
Isothiocyanates (ITCs), present as glucosinolate precursors in cruciferous vegetables, have shown anti-inflammatory, antioxidant and anticarcinogenic activities. Here, we compared the effects of three different ITCs on ROS production and on the expression of matrix metalloproteinase (MMP)-2 and -9, which represent important pathogenetic factors of various neurological diseases. Primary cultures of rat astrocytes were activated by LPS and simultaneously treated with different doses of Allyl isothiocyanate (AITC), 2-Phenethyl isothiocyanate (PEITC) and 2-Sulforaphane (SFN). Results showed that SFN and PEITC were able to counteract ROS production induced by H2O2. The zymographic analysis of cell culture supernatants evidenced that PEITC and SFN were the most effective inhibitors of MMP-9, whereas, only SFN significantly inhibited MMP-2 activity. PCR analysis showed that all the ITCs used significantly inhibited both MMP-2 and MMP-9 expression. The investigation on the mitogen-activated protein kinase (MAPK) signaling pathway demonstrated that ITCs modulate MMP transcription by inhibition of extracellular-regulated protein kinase (ERK) activity. Results of this study suggest that ITCs could be promising nutraceutical agents for the prevention and complementary treatment of neurological diseases associated with MMP involvement
Sex-differences in the longitudinal recovery of neuromuscular function in COVID-19 associated acute respiratory distress syndrome survivors
Introduction: Patients admitted to the intensive care unit (ICU) following severe acute respiratory syndrome 2 (SARS-CoV-2) infection may have muscle weakness up to 1 year or more following ICU discharge. However, females show greater muscle weakness than males, indicating greater neuromuscular impairment. The objective of this work was to assess sex differences in longitudinal physical functioning following ICU discharge for SARS-CoV-2 infection. Methods: We performed longitudinal assessment of physical functioning in two groups: 14 participants (7 males, 7 females) in the 3-to-6 month and 28 participants (14 males, 14 females) in the 6-to-12 month group following ICU discharge and assessed differences between the sexes. We examined self-reported fatigue, physical functioning, compound muscle action potential (CMAP) amplitude, maximal strength, and the neural drive to the tibialis anterior muscle. Results: We found no sex differences in the assessed parameters in the 3-to-6-month follow-up, indicating significant weakness in both sexes. Sex differences emerged in the 6-to-12-month follow-up. Specifically, females exhibited greater impairments in physical functioning, including lower strength, walking lower distances, and high neural input even 1 year following ICU-discharge. Discussion: Females infected by SARS-CoV-2 display significant impairments in functional recovery up to 1 year following ICU discharge. The effects of sex should be considered in post-COVID neurorehabilitation
High surface area mesoporous silica nanoparticles with tunable size in the sub-micrometer regime: Insights on the size and porosity control mechanisms
Mesoporous silica nanostructures (MSNs) attract high interest due to their unique and tunable physical chemical features, including high specific surface area and large pore volume, that hold a great potential in a variety of fields, i.e., adsorption, catalysis, and biomedicine. An essential feature for biomedical application of MSNs is limiting MSN size in the sub-micrometer regime to control uptake and cell viability. However, careful size tuning in such a regime remains still chal-lenging. We aim to tackling this issue by developing two synthetic procedures for MSN size mod-ulation, performed in homogenous aqueous/ethanol solution or two-phase aqueous/ethyl acetate system. Both approaches make use of tetraethyl orthosilicate as precursor, in the presence of cetyltri-methylammonium bromide, as structure-directing agent, and NaOH, as base-catalyst. NaOH catalyzed syntheses usually require high temperature (>80 °C) and large reaction medium volume to trigger MSN formation and limit aggregation. Here, a successful modulation of MSNs size from 40 up to 150 nm is demonstrated to be achieved by purposely balancing synthesis conditions, being able, in addition, to keep reaction temperature not higher than 50 °C (30 °C and 50 °C, respectively) and reaction mixture volume low. Through a comprehensive and in-depth systematic morphologi-cal and structural investigation, the mechanism and kinetics that sustain the control of MSNs size in such low dimensional regime are defined, highlighting that modulation of size and pores of the structures are mainly mediated by base concentration, reaction time and temperature and ageing, for the homogenous phase approach, and by temperature for the two-phase synthesis. Finally, an in vitro study is performed on bEnd.3 cells to investigate on the cytotoxicity of the MNSs
COVID-19 affects serum brain-derived neurotrophic factor and neurofilament light chain in aged men. Implications for morbidity and mortality
Background and Methods: Severe COVID-19 is known to induce neurological damage (NeuroCOVID), mostly in aged individuals, by affecting brain-derived neurotrophic factor (BDNF), matrix metalloproteinases (MMP) 2 and 9 and the neurofilament light chain (NFL) pathways. Thus, the aim of this pilot study was to investigate BDNF, MMP-2, MMP-9, and NFL in the serum of aged men affected by COVID-19 at the beginning of the hospitalization period and characterized by different outcomes, i.e., attending a hospital ward or an intensive care unit (ICU) or with a fatal outcome. As a control group, we used a novelty of the study, unexposed age-matched men. We also correlated these findings with the routine blood parameters of the recruited individuals. Results: We found in COVID-19 individuals with severe or lethal outcomes disrupted serum BDNF, NFL, and MMP-2 presence and gross changes in ALT, GGT, LDH, IL-6, ferritin, and CRP. We also confirmed and extended previous data, using ROC analyses, showing that the ratio MMPs (2 and 9) versus BDNF and NFL might be a useful tool to predict a fatal COVID-19 outcome. Conclusions: Serum BDNF and NFL and/or their ratios with MMP-2 and MMP-9 could represent early predictors of NeuroCOVID in aged men
On possible interpretations of the high energy electron-positron spectrum measured by the Fermi Large Area Telescope
The Fermi-LAT experiment recently reported high precision measurements of the
spectrum of cosmic-ray electrons-plus-positrons (CRE) between 20 GeV and 1 TeV.
The spectrum shows no prominent spectral features, and is significantly harder
than that inferred from several previous experiments. Here we discuss several
interpretations of the Fermi results based either on a single large scale
Galactic CRE component or by invoking additional electron-positron primary
sources, e.g. nearby pulsars or particle Dark Matter annihilation. We show that
while the reported Fermi-LAT data alone can be interpreted in terms of a single
component scenario, when combined with other complementary experimental
results, specifically the CRE spectrum measured by H.E.S.S. and especially the
positron fraction reported by PAMELA between 1 and 100 GeV, that class of
models fails to provide a consistent interpretation. Rather, we find that
several combinations of parameters, involving both the pulsar and dark matter
scenarios, allow a consistent description of those results. We also briefly
discuss the possibility of discriminating between the pulsar and dark matter
interpretations by looking for a possible anisotropy in the CRE flux.Comment: 29 pages, 12 figures. Final version accepted for publication in
Astroparticle Physic
Fermi LAT Observations of the Supernova Remnant W28 (G6.4-0.1)
We present detailed analysis of the two gamma-ray sources,1FGL J1801.3-2322c
and 1FGL J1800.5-2359c,that have been found toward the supernova remnant(SNR)
W28 with the Large Area Telescope(LAT) on board the Fermi Gamma-ray Space
Telescope.1FGL J1801.3-2322c is found to be an extended source within the
boundary of SNR W28,and to extensively overlap with the TeV gamma-ray source
HESS J1801-233,which is associated with a dense molecular cloud interacting
with the supernova remnant.The gamma-ray spectrum measured with LAT from
0.2--100 GeV can be described by a broken power-law function with a break of
~1GeV,and photon indices of 2.090.08(stat)0.28(sys) below the break
and 2.740.06(stat)0.09(sys) above the break.Given the clear
association between HESS J1801-233 and the shocked molecular cloud and a
smoothly connected spectrum in the GeV--TeV band,we consider the origin of the
gamma-ray emission in both GeV and TeV ranges to be the interaction between
particles accelerated in the SNR and the molecular cloud.The decay of neutral
pions produced in interactions between accelerated hadrons and dense molecular
gas provide a reasonable explanation for the broadband gamma-ray spectrum. 1FGL
J1800.5-2359c, located outside the southern boundary of SNR W28, cannot be
resolved.An upper limit on the size of the gamma-ray emission was estimated to
be ~16 using events above ~2GeV under the assumption of a circular shape
with uniform surface brightness. It appears to coincide with the TeV source
HESS J1800-240B,which is considered to be associated with a dense molecular
cloud that contains the ultra compact HII region W28A2(G5.89-0.39).We found no
significant gamma-ray emission in the LAT energy band at the positions of TeV
sources HESS J1800-230A and HESS J1800-230C.The LAT data for HESS J1800-230A
combined with the TeV data points indicate a spectral break between 10GeV and
100GeV.Comment: 23 pages, 6 figures. Accepted for publication in the Astrophysical
Journal. Corresponding authors: H. Katagiri, H. Tajima, T. Tanaka, and Y.
Uchiyam
GeV Gamma-ray Flux Upper Limits from Clusters of Galaxies
The detection of diffuse radio emission associated with clusters of galaxies
indicates populations of relativistic leptons infusing the intracluster medium.
Those electrons and positrons are either injected into and accelerated directly
in the intracluster medium, or produced as secondary pairs by cosmic-ray ions
scattering on ambient protons. Radiation mechanisms involving the energetic
leptons together with decay of neutral pions produced by hadronic interactions
have the potential to produce abundant GeV photons. Here, we report on the
search for GeV emission from clusters of galaxies using data collected by the
Large Area Telescope (LAT) on the Fermi Gamma-ray Space Telescope (Fermi) from
August 2008 to February 2010. Thirty-three galaxy clusters have been selected
according to their proximity and high mass, X-ray flux and temperature, and
indications of non-thermal activity for this study. We report upper limits on
the photon flux in the range 0.2-100 GeV towards a sample of observed clusters
(typical values 1-5 x 10^-9 ph cm^-2 s^-1) considering both point-like and
spatially resolved models for the high-energy emission, and discuss how these
results constrain the characteristics of energetic leptons and hadrons, and
magnetic fields in the intracluster medium. The volume-averaged
relativistic-hadron-to-thermal energy density ratio is found to be < 5-10% in
several clusters.Comment: 9 pages, 3 tables, 1 figure, accepted for publication in ApJ Letter
Searches for Cosmic-Ray Electron Anisotropies with the Fermi Large Area Telescope
The Large Area Telescope on board the \textit{Fermi} satellite
(\textit{Fermi}-LAT) detected more than 1.6 million cosmic-ray
electrons/positrons with energies above 60 GeV during its first year of
operation. The arrival directions of these events were searched for
anisotropies of angular scale extending from 10 up to
90, and of minimum energy extending from 60 GeV up to 480 GeV. Two
independent techniques were used to search for anisotropies, both resulting in
null results. Upper limits on the degree of the anisotropy were set that
depended on the analyzed energy range and on the anisotropy's angular scale.
The upper limits for a dipole anisotropy ranged from to .Comment: 16 pages, 10 figures, accepted for publication in Physical Review D -
contact authors: M.N. Mazziotta and V. Vasileio
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