Use of Fluorescence Microscopy in an Assay of Sperm Density for the Gorgonian Coral, Plexaura kuna

The density of sperm in the water column during the broadcast spawning events of marine invertebrates is often undetermined or reported in terms of fertilization potential. The density of sperm during such events can be determined by directly counting numbers of spermatozoa using a modification of the acridine orange direct count (AODC) technique for enumerating bacteria. A number of variables in the handling and processing of samples may bias estimates. Sample collection in glass and rapid fixation and filtration are necessary for reproducible estimates. Once filtered, samples are stable for many months, and counts on filters that were poorly stained in the field can be enhanced by additional staining with 4\u276-diamidino-2-phynlindole (DAPI). The AODC technique was employed to examine the effects of distance from a male colony on density of sperm for the gorgonian coral Plexaura kuna. In situ sperm densities ranged from 0 to 1,000 sperm/ml. Sperm densities in 18 l aquaria containing 20 cm long P. kuna explants reached 100,000 sperm/ml

Pictor A (PKS 0518-45) - From Nucleus to Lobes

We present radio and optical imaging and kinematic data for the radio galaxy Pictor A, including HST continuum and [OIII], emission-line images (at a resolution of 25 - 100 mas) and ground-based imaging and spectroscopy (at a resolution of ~ 1.5". The radio data include 3 cm Australia Telescope images of the core, at a resolution comparable to that of the optical, ground-based images, and a VLBI image of a jet in the compact core (at a resolution of 2 - 25 mas), which seems to align with a continuum ``jet'' found in the HST images. The core radio jet, the HST optical continuum ``jet'', and the NW H-alpha filaments all appear to point toward the optical-synchrotron hot-spot in the NW lobe of this object and are associated with a disrupted velocity field in the extended ionized gas. The ground-based spectra which cover this trajectory also yield line ratios for the ionized gas which have anomalously low [NII] (6564), suggesting either a complex, clumpy structure in the gas with a higher cloud-covering factor at larger radii and with denser clouds than is found in the nuclear regions of most NLRG and Seyfert 2 galaxies, or some other, unmodeled, mechanism for the emergent spectrum from this region. The H-alpha emission-line filaments to the N appear to be associated with a 3 cm radio continuum knot which lies in a gap in the filaments ~ 4" from the nucleus. Altogether, the data in this paper provide good circumstantial evidence for non-disruptive redirection of a radio jet by interstellar gas clouds in the host galaxy.Comment: 19 pages, 6 ps.gz fig pages, to appear in the Ap.J. Supp

XMM-Newton observation of PSR B2224+65 and its jet

We have investigated the pulsar PSR B2224+65 and its X-ray jet with XMM-Newton. Apart from the long X-ray jet which is almost perpendicular to the direction of proper motion, a putative extended feature at the pulsar position, which oriented in the opposite direction of the proper motion, is also suggested by this deep X-ray imaging. Non-detection of any coherent X-ray pulsation disfavors the magnetospheric origin of the X-rays observed from the position of PSR B2224+65 and hence suggest that the interpretation of pulsar wind nebula is more viable. We have also probed the origin of PSR B2224+65 and identified a runaway star, which possibly originated from the Cygnus OB9 association, as a candidate for the former binary companion of the neutron star's progenitor.Comment: 24 pages, 8 figures, 3 tables, accepted for publication in Ap

The Hubble Legacy Archive NICMOS Grism Data

The Hubble Legacy Archive (HLA) aims to create calibrated science data from the Hubble Space Telescope archive and make them accessible via user-friendly and Virtual Observatory (VO) compatible interfaces. It is a collaboration between the Space Telescope Science Institute (STScI), the Canadian Astronomy Data Centre (CADC) and the Space Telescope - European Coordinating Facility (ST-ECF). Data produced by the Hubble Space Telescope (HST) instruments with slitless spectroscopy modes are among the most difficult to extract and exploit. As part of the HLA project, the ST-ECF aims to provide calibrated spectra for objects observed with these HST slitless modes. In this paper, we present the HLA NICMOS G141 grism spectra. We describe in detail the calibration, data reduction and spectrum extraction methods used to produce the extracted spectra. The quality of the extracted spectra and associated direct images is demonstrated through comparison with near-IR imaging catalogues and existing near-IR spectroscopy. The output data products and their associated metadata are publicly available through a web form at http://hla.stecf.org and via VO interfaces. In total, 2470 spectra of 1923 unique targets are included in the current release.Comment: 18 pages, 21 figures, accepted for publication in Astronomy & Astrophysic

The Hubble Legacy Archive ACS Grism Data

A public release of slitless spectra, obtained with ACS/WFC and the G800L grism, is presented. Spectra were automatically extracted in a uniform way from 153 archival fields (or "associations") distributed across the two Galactic caps, covering all observations to 2008. The ACS G800L grism provides a wavelength range of 0.55-1.00 \mu$m, with a dispersion of$40 \ \AA / pixel$and a resolution of$\sim 80\ \AA$for point-like sources. The ACS G800L images and matched direct images were reduced with an automatic pipeline that handles all steps from archive retrieval, alignment and astrometric calibration, direct image combination, catalogue generation, spectral extraction and collection of metadata. The large number of extracted spectra (73,581) demanded automatic methods for quality control and an automated classification algorithm was trained on the visual inspection of several thousand spectra. The final sample of quality controlled spectra includes 47,919 datasets (65of extracted spectra) for$32,149$unique objects, with a median$i_{\rm AB}$-band magnitude of 23.7, reaching 26.5 AB for the faintest objects. Each released dataset contains science-ready 1D and 2D spectra, as well as multi-band image cutouts of corresponding sources and a useful preview page summarising the direct and slitless data, astrometric and photometric parameters. In order to characterize the slitless spectra, emission-line flux and equivalent width sensitivity of the ACS data were compared with public ground-based spectra in the GOODS-South field. An example list of emission line galaxies with two or more identified lines is also included, covering the redshift range$0.2-4.6$.Comment: Accepted for publication in Astronomy and Astrophysics; 29 pages, 16 Figures, 4 Tables in text and 3Tables in Appendi

The Large Quasar Reference Frame (LQRF) - an optical representation of the ICRS

The large number and all-sky distribution of quasars from different surveys, along with their presence in large, deep astrometric catalogs,enables the building of an optical materialization of the ICRS following its defining principles. Namely: that it is kinematically non-rotating with respect to the ensemble of distant extragalactic objects; aligned with the mean equator and dynamical equinox of J2000; and realized by a list of adopted coordinates of extragalatic sources. Starting from the updated and presumably complete LQAC list of QSOs, the initial optical positions of those quasars are found in the USNO B1.0 and GSC2.3 catalogs, and from the SDSS DR5. The initial positions are next placed onto UCAC2-based reference frames, following by an alignment with the ICRF, to which were added the most precise sources from the VLBA calibrator list and the VLA calibrator list - when reliable optical counterparts exist. Finally, the LQRF axes are inspected through spherical harmonics, contemplating to define right ascension, declination and magnitude terms. The LQRF contains J2000 referred equatorial coordinates for 100,165 quasars, well represented across the sky, from -83.5 to +88.5 degrees in declination, and with 10 arcmin being the average distance between adjacent elements. The global alignment with the ICRF is 1.5 mas, and the individual position accuracies are represented by a Poisson distribution that peaks at 139 mas in right ascension and 130 mas in declination. It is complemented by redshift and photometry information from the LQAC. The LQRF is designed to be an astrometric frame, but it is also the basis for the GAIA mission initial quasars' list, and can be used as a test bench for quasars' space distribution and luminosity function studies.Comment: 23 pages, 23 figures, 6 tables Accepted for publication by Astronomy & Astrophysics, on 25 May 200

Putting the Pieces Together: Integrative Modeling Platform Software for Structure Determination of Macromolecular Assemblies

A set of software tools for building and distributing models of macromolecular assemblies uses an integrative structure modeling approach, which casts the building of models as a computational optimization problem where information is encoded into a scoring function used to evaluate candidate models

The JCMT Nearby Galaxies Legacy Survey VII: H\alpha{} imaging and massive star formation properties

We present H\alpha{} fluxes, star formation rates (SFRs) and equivalent widths (EWs) for a sample of 156 nearby galaxies observed in the 12CO J=3-2 line as part of the James Clerk Maxwell Telescope Nearby Galaxies Legacy Survey. These are derived from images and values in the literature and from new H\alpha{} images for 72 galaxies which we publish here. We describe the sample, observations and procedures to extract the H\alpha{} fluxes and related quantities. We discuss the SFR properties of our sample and confirm the well-known correlation with galaxy luminosity, albeit with high dispersion. Our SFRs range from 0.1 to 11 Msun yr-1 with a median SFR value for the complete sample of 0.2 Msun yr-1. This median values is somewhat lower than similar published measurements, which we attribute, in part, to our sample being HI-selected and, thus, not biased towards high SFRs as has frequently been the case in previous studies. Additionally, we calculate internal absorptions for the H\alpha{} line, A(H\alpha{}), which are lower than many of those used in previous studies. Our derived EWs, which range from 1 to 880\AA{} with a median value of 27\AA{}, show little dependence with luminosity but rise by a factor of five from early- to late-type galaxies. This paper is the first in a series aimed at comparing SFRs obtained from H\alpha{} imaging of galaxies with information derived from other tracers of star formation and atomic and molecular gas.Comment: Accepted for publication in MNRAS. 47 pages, 18 figure