4,638 research outputs found
Fabrication of calcium phosphate microcapsules using emulsion droplets stabilized with branched copolymers as templates
We report on a versatile and time-efficient method to fabricate calcium phosphate (CaP) microcapsules by utilizing oil-in-water emulsion droplets stabilized with synthetic branched copolymer (BCP) as templates. The BCP was designed to provide a suitable architecture and functionality to produce stable emulsion droplets, and to permit the mineralization of CaP at the surface of the oil droplet when incubated in a solution containing calcium and phosphate ions. The CaP shells of the microcapsules were established to be calcium deficient hydroxyapatite with incorporated chlorine and carbonate species. These capsule walls were made fluorescent by decoration with a fluorescein-bisphosphonate conjugate
Phenoloxidase activity acts as a mosquito innate immune response against infection with semliki forest virus
Several components of the mosquito immune system including the RNA interference (RNAi), JAK/STAT, Toll and IMD pathways have previously been implicated in controlling arbovirus infections. In contrast, the role of the phenoloxidase (PO) cascade in mosquito antiviral immunity is unknown. Here we show that conditioned medium from the Aedes albopictus-derived U4.4 cell line contains a functional PO cascade, which is activated by the bacterium Escherichia coli and the arbovirus Semliki Forest virus (SFV) (Togaviridae; Alphavirus). Production of recombinant SFV expressing the PO cascade inhibitor Egf1.0 blocked PO activity in U4.4 cell- conditioned medium, which resulted in enhanced spread of SFV. Infection of adult female Aedes aegypti by feeding mosquitoes a bloodmeal containing Egf1.0-expressing SFV increased virus replication and mosquito mortality. Collectively, these results suggest the PO cascade of mosquitoes plays an important role in immune defence against arboviruses
An XMM-Newton view of the `bare' nucleus of Fairall 9
We present the spectral results from a 130 ks observation, obtained from the
X-ray Multi-Mirror Mission-Newton (XMM-Newton) observatory, of the type I
Seyfert galaxy Fairall 9. An X-ray hardness-ratio analysis of the light-curves,
reveals a `softer-when-brighter' behaviour which is typical for radio-quiet
type I Seyfert galaxies. Moreover, we analyse the high spectral-resolution data
of the reflection grating spectrometer and we did not find any significant
evidence supporting the presence of warm-absorber in the low X-ray energy part
of the source's spectrum. This means that the central nucleus of Fairall 9 is
`clean' and thus its X-ray spectral properties probe directly the physical
conditions of the central engine. The overall X-ray spectrum in the 0.5-10 keV
energy-range, derived from the EPIC data, can be modelled by a relativistically
blurred disc-reflection model. This spectral model yields for Fairall 9 an
intermediate black-hole best-fit spin parameter of
.Comment: Accepted for publication in MNRAS. The paper contains 11 figures and
1 tabl
MICU1 Controls Both the Threshold and Cooperative Activation of the Mitochondrial Ca(2+) Uniporter.
Mitochondrial Ca(2+) uptake via the uniporter is central to cell metabolism, signaling, and survival. Recent studies identified MCU as the uniporter\u27s likely pore and MICU1, an EF-hand protein, as its critical regulator. How this complex decodes dynamic cytoplasmic [Ca(2+)] ([Ca(2+)]c) signals, to tune out small [Ca(2+)]c increases yet permit pulse transmission, remains unknown. We report that loss of MICU1 in mouse liver and cultured cells causes mitochondrial Ca(2+) accumulation during small [Ca(2+)]c elevations but an attenuated response to agonist-induced [Ca(2+)]c pulses. The latter reflects loss of positive cooperativity, likely via the EF-hands. MICU1 faces the intermembrane space and responds to [Ca(2+)]c changes. Prolonged MICU1 loss leads to an adaptive increase in matrix Ca(2+) binding, yet cells show impaired oxidative metabolism and sensitization to Ca(2+) overload. Collectively, the data indicate that MICU1 senses the [Ca(2+)]c to establish the uniporter\u27s threshold and gain, thereby allowing mitochondria to properly decode different inputs
Mid-Infrared Properties of the Swift Burst Alert Telescope Active Galactic Nuclei Sample of the Local Universe. I. Emission-Line Diagnostics
We compare mid-infrared emission-line properties, from high-resolution
Spitzer spectra of a hard X-ray (14 -- 195 keV) selected sample of nearby (z <
0.05) AGN detected by the Burst Alert Telescope (BAT) aboard Swift. The
luminosity distribution for the mid-infrared emission-lines, [O IV] 25.89
micron, [Ne II] 12.81 micron, [Ne III] 15.56 micron and [Ne V] 14.32/24.32
micron, and hard X-ray continuum show no differences between Seyfert 1 and
Seyfert 2 populations, however six newly discovered BAT AGNs are under-luminous
in [O IV], most likely the result of dust extinction in the host galaxy. The
overall tightness of the mid-infrared correlations and BAT fluxes and
luminosities suggests that the emission lines primarily arise in gas ionized by
the AGN. We also compare the mid-infrared emission-lines in the BAT AGNs with
those from published studies of ULIRGs, PG QSOs, star-forming galaxies and
LINERs. We find that the BAT AGN sample fall into a distinctive region when
comparing the [Ne III]/[Ne II] and the [O IV]/[Ne III] ratios. These line
ratios are lower in sources that have been previously classified in the
mid-infrared/optical as AGN than those found for the BAT AGN, suggesting that,
in our X-ray selected sample, the AGN represents the main contribution to the
observed line emission. These ratios represent a new emission line diagnostic
for distinguishing between AGN and star forming galaxies.Comment: 54 pages, 9 Figures. Accepted for publication in The Astrophysical
Journal
On twisted Fourier analysis and convergence of Fourier series on discrete groups
We study norm convergence and summability of Fourier series in the setting of
reduced twisted group -algebras of discrete groups. For amenable groups,
F{\o}lner nets give the key to Fej\'er summation. We show that Abel-Poisson
summation holds for a large class of groups, including e.g. all Coxeter groups
and all Gromov hyperbolic groups. As a tool in our presentation, we introduce
notions of polynomial and subexponential H-growth for countable groups w.r.t.
proper scale functions, usually chosen as length functions. These coincide with
the classical notions of growth in the case of amenable groups.Comment: 35 pages; abridged, revised and update
Repeated cleavage failure does not establish centrosome amplification in untransformed human cells
Transient cleavage failure in dividing cells is not sufficient to establish stable populations of cells with extra centrosomes
A formation scenario of young stellar groups in the region of the Scorpio Centaurus OB association
The main objective of this work is to investigate the role played by Lower
Centaurus Crux (LCC) and Upper Centaurus Lupus (UCL), both subcomponents of the
Scorpio Centaurus OB association (Sco-Cen), in the formation of the groups beta
Pictoris, TW Hydrae and the eta Chamaeleontis cluster. The dynamical evolution
of all the stellar groups involved and of the bubbles and shells blown by LCC
and UCL are calculated and followed from the past to the present. This leads to
a formation scenario in which (1) the groups beta Pictoris, TW Hydrae were
formed in the wake of the shells created by LCC and UCL, (2) the young cluster
eta Chamaeleontis was born as a consequence of the collision of the shells of
LCC and UCL, and (3) the formation of Upper Scorpius (US), the other main
subcomponent of the Sco-Cen association, may have been started by the same
process that created eta Chamaeleontis
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