Quality Of Seeds Of Jatobá-do-cerrado Processed And Stored In Diferents Forms

The preservation of the physical and chemical quality of jatobá-do-cerrado seeds during processing and storage is essential to the restocking of vegetation in degraded areas. Since no scientific studies have analysed the optimal post-harvest conditions for jatobá-do-cerrado seeds, this study aimed to evaluate the physical and chemical quality of jatobá-do-cerrado seeds following different types of processing: with pulp, without pulp (scarification), without pulp (fermentation) and at different storage temperatures (10 and 23 °C) and different packaging (tetrapack, paper, plastic, PET bottles and glass bottles), over six months. The physical and chemical constituents of jatobá-do-cerrado seeds varied according to the packaging conditions. An increased storage time reduced the quality of the seeds. Seeds with pulp showed better physical characteristics during storage. The pulping processing of seeds by mechanical scarification increased the acidity and ash content, but led to the highest percentage of crude protein during storage at 23 °C. The fermentation method of seed pulping positively affected seed quality during storage at 10 °C. Permeable packaging (paper bags and tetrapack) led to a greater reduction in seed quality than that in glass bottles, PET bottles or plastic bags. The best conditions for the processing of jatobá-do-cerrado seeds was fermentation and the best storage condition was in waterproof packaging (glass or PET bottles).37266568

Fire frequency analysis in Portugal (1975-2005), using Landsat-based burnt area maps

Fire frequency in 21 forest planning regions of Portugal during the period 1975–2005 was estimated from historical burnt area maps generated with semi-automatic classification of Landsat Thematic Mapper (TM) satellite imagery. Fire return interval distributions were modelled with the Weibull function and the estimated parameters were used to calculate regional mean, median and modal fire return intervals, as well as regional hazard functions. Arrangement of the available data into three different time series allowed for assessment of the effects of minimum mapping unit, time series length and use of censored data on the Weibull function parameter estimates. Varying the minimum mapping unit between 5 and 35 ha had a negligible effect on parameter estimates, whereas changing the time series length from 22 to 31 years substantially affected the estimates. However, the strongest effect was caused by censored data. Its exclusion led to substantial overestimation of fire frequency and of burning probability dependence on fuel age. We estimated a countrywide mean fire interval of 36 years and an annual burnt area of 1.2%. Regional variations in fire frequency descriptors were interpreted in terms of land cover and land use practices that affect the contemporary fire regime in Portugal

Transporte, fluxo de mercadoria e desenvolvimento econômico urbano na Amazônia: o caso de Belém e Manaus

This paper addresses the fraught relationships among commodity trade, urban economic development and the environment in the world’s largest rainforest reserve, in a historical narrative fashion. The conceptual framework in which we position this narrative is provided by Hesse (2010), in the “site” and “situation” dimensions of the interaction between places or locales on the one hand, and material flows or global value chains on the other. It is argued that the assemblage of both site and situation is what shapes the wealth of cities. The case study of Manaus and Belém shows how the rapid urbanization of the Amazon rainforest is accompanied by the growth of shipping as “new” commodities are being extracted from the jungle interior

In vivo and in vitro heat shock proteins gene expression in cattle.

The main purpose for this study was the quantification of the heat shock proteins HSPA1A and HSP90AA1, in cow lymphocytes, when subjected to heat stress directly - in vivo, or indirectly - in vitro. The aim was to identify differences between HSP expression in vitro and in vivo. The experiment was conducted in the Biometeorology and Ethology Laboratory of FZEA-USP. Were used three female Holstein Frisian, which were subjected to heat stress, by sun exposure. The blood samples were collected after sun exposure, with a temperature of 40 ± 2 º C, during three days. For in vitro tests, blood of the same animals was collected and placed for a period of 4 hours in a water bath at 40 º C, thus simulating the thermal stress. Total RNA of lymphocytes was extracted, treated with DNase I and submitted to cDNA synthesis for gene expression quantification of HSPA1A and HSP90AA1, by real time PCR (qRT-PCR). The data were tested for normality by Kolmogorov-Smirnov test and for homocedasticity by Levene test. Data were analyzed according to a general linear model procedure with 2 fixed factors treatment and genes expression. Significantly different means were submitted to post-hoc comparisons of means (LSD test) and regarded as significantly different when P<0.05. The results showed that there are no significant differences between the in vitro and the in vivo treatments

Potent Trivalent Inhibitors of Thrombin through Hybridization of Salivary Sulfopeptides from Hematophagous Arthropods

Blood feeding arthropods, such as leeches, ticks, flies and mosquitoes, provide a privileged source of peptidic anticoagulant molecules. These primarily operate through inhibition of the central coagulation protease thrombin by binding to the active site and either exosite I or exosite II. Herein, we describe the rational design of a novel class of trivalent thrombin inhibitors that simultaneously block both exosites as well as the active site. These engineered hybrids were synthesized using tandem diselenide-selenoester ligation (DSL) and native chemical ligation (NCL) reactions in one-pot. The most potent trivalent inhibitors possessed femtomolar inhibition constants against alpha-thrombin and were selective over related coagulation proteases. A lead hybrid inhibitor possessed potent anticoagulant activity, blockade of both thrombin generation and platelet aggregation in vitro and efficacy in a murine thrombosis model at 1 mg kg(-1). The rational engineering approach described here lays the foundation for the development of potent and selective inhibitors for a range of other enzymatic targets that possess multiple sites for the disruption of protein-protein interactions, in addition to an active site

Nutritional Evaluation Of Children With Chronic Cholestatic Disease

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)To evaluate the nutritional status of children with persistent cholestasis and to compare the anthropometric indices between children with and without liver cirrhosis and children with and without jaundice. Methods Children with persistent cholestasis, i.e. increased direct bilirrubin or changes in the canalicular enzyme gamma-glutamyl transferase (GGT), were included. The anthropometric measures were weight (W), height or length (H), arm circumference (AC), triceps skinfold thickness (TST), arm muscle circumference (AMC), and body mass index (BMI). Results Ninety-one children with cholestasis, with current median age of 12 months, were evaluated. W/age (A) and H/A indices below -2 Z-scores were observed in 33% and 30.8% of patients, respectively. Concerning the W/H index and BMI, only 12% and 16% of patients, respectively, were below -2 Z-scores. Regarding AC, 43.8% of 89 evaluated patients had some depletion. Observing the TST, 64% of patients had depletion, and 71.1% of the 45 evaluated patients had some degree of depletion regarding the ACM index. Conclusion Evaluation using weight in patients with chronic liver diseases may overestimate the nutritional status due to visceromegaly, subclinical edema, or ascites. Indices that correlate weight and height, such as W/H and BMI, may also not show depletion because of the chronic condition in which there are depletion of both weight and height. TST, AC, and ACM are parameters that better estimate nutritional status and should be part of the management of patients with liver diseases and cholestasis. © 2015 Sociedade Brasileira de Pediatria.922197205CNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Gene expression in placentation of farm animals: An overview of gene function during development

AbstractEutherian mammals share a common ancestor that evolved into two main placental types, i.e., hemotrophic (e.g., human and mouse) and histiotrophic (e.g., farm animals), which differ in invasiveness. Pregnancies initiated with assisted reproductive techniques (ART) in farm animals are at increased risk of failure; these losses were associated with placental defects, perhaps due to altered gene expression. Developmentally regulated genes in the placenta seem highly phylogenetically conserved, whereas those expressed later in pregnancy are more species-specific. To elucidate differences between hemotrophic and epitheliochorial placentae, gene expression data were compiled from microarray studies of bovine placental tissues at various stages of pregnancy. Moreover, an in silico subtractive library was constructed based on homology of bovine genes to the database of zebrafish — a nonplacental vertebrate. In addition, the list of placental preferentially expressed genes for the human and mouse were collected using bioinformatics tools (Tissue-specific Gene Expression and Regulation [TiGER] — for humans, and tissue-specific genes database (TiSGeD) — for mice and humans). Humans, mice, and cattle shared 93 genes expressed in their placentae. Most of these were related to immune function (based on analysis of gene ontology). Cattle and women shared expression of 23 genes, mostly related to hormonal activity, whereas mice and women shared 16 genes (primarily sexual differentiation and glycoprotein biology). Because the number of genes expressed by the placentae of both cattle and mice were similar (based on cluster analysis), we concluded that both cattle and mice were suitable models to study the biology of the human placenta

IKK-induced NF-kappa B1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen

The importance of IκB kinase (IKK)–induced proteolysis of NF-κB1 p105 in B cells was investigated using Nfkb1SSAA/SSAA mice, in which this NF-κB signaling pathway is blocked. Nfkb1SSAA mutation had no effect on the development and homeostasis of follicular mature (FM) B cells. However, analysis of mixed bone marrow chimeras revealed that Nfkb1SSAA/SSAA FM B cells were completely unable to mediate T cell–dependent antibody responses. Nfkb1SSAA mutation decreased B cell antigen receptor (BCR) activation of NF-κB in FM B cells, which selectively blocked BCR stimulation of cell survival and antigen-induced differentiation into plasmablasts and germinal center B cells due to reduced expression of Bcl-2 family proteins and IRF4, respectively. In contrast, the antigen-presenting function of FM B cells and their BCR-induced migration to the follicle T cell zone border, as well as their growth and proliferation after BCR stimulation, were not affected. All of the inhibitory effects of Nfkb1SSAA mutation on B cell functions were rescued by normalizing NF-κB activation genetically. Our study identifies critical B cell-intrinsic functions for IKK-induced NF-κB1 p105 proteolysis in the antigen-induced survival and differentiation of FM B cells, which are essential for T-dependent antibody responses