Improved inoculants for lentil

Non-Peer ReviewedThe effectiveness of the available commercial inoculants for lentil, Rhizogen, Enftx-L (Esso), Nitragin 'C', and Grip (Inotec) were compared with three inoculants which contained the rhizobia strains of 99A1, ICAR 20, and 92A3. Lentil inoculated with a sterile inoculant was used as the control. Total dry matter, total grain yield, percent total N and total N was determined At seven sites, an average grain yield for lentil was observed whereas for two sites yield was below average. Inoculant containing 99A1 and ICAR 20 an overall increase in grain yield by 14 % as compared with Nitragin 'C', strain 99A3 with 9 %, and Enftx-L with 7 %. The average grain yield of all nine sites for lentil inoculated with Grip and Rhizogen showed an insignificant decrease of 2 and 3 % as compared with lentil inoculated with Nitragin 'C'

Evaluation of the effectiveness of Rhizobium leguminosarum strains for pea under field conditions

Non-Peer ReviewedOne hundred and eight isolates of Rhizobium leguminosarum were screened for effectiveness for pea under controlled environments. Eight superior strains plus the commercially available pea inoculants, Nitragin 'C', Grip-Inotec, and Rhizogen were tested at Waldheim and Brooksby for effectiveness with Tipu and Trapper pea as host plants. The experiment was a RCBD, laid out as a split plot with the two pea varieties as main plot treatments and rhizobia! strain as the subplot treatments, replicated four times. At Waldheim total dry matter ranged from 2429 kg/ha for uninoculated Trapper to 4024 kg/ha for Tipu inoculated with strain 128C79. Grain yield ranged from 1046 kg/ha for Trapper inoculated with strain 175G3 to 1665 kg/ha for Tipu inoculated with strain 175G3. Total grain-N ranged from 33.7 kg/ha for uninoculated Tipu to 55.5 kg/ha for. Trapper inoculated with strain 128C56G. At this site, strain 175G3 appears to be a superior strain for Tipu but is the least effective strain tested for Trapper. At Brooks by, no significant differences due to strain or cultivar were observed. Values for total dry matter, grain yield, and total N were around 75 % of those values found at Waldheim. A survey to assess nodulation was carried out and all uninoculated pea were nodulated. This would indicate the presence of indigenous R. leguminosarum nodulating pea or possible cross contamination from adjacent plots. The below average yield and the absence of a yield response due to inoculation can largely be attributed to the extreme dry weather occurring at both sites. Nodulation of the uninoculated control has also reduced the effect of inoculation on yield

The influence of host and non-host crops on the rhizobial population of the root rhizosphere

Non-Peer ReviewedField pea (Pisum sativum), a new crop to the Dark Brown and Black Chernozemic Soil Zones of Saskatchewan, forms a symbiosis with Rhizobium leguminosarum biovar viceae. This rhizobium is not native to the region, yet numbers in excess of 10^4 g-1 soil have been observed several years after a single inoculated field pea crop was grown. Modified immunoblot and ELISA techniques utilizing strain-specific polyclonal antibodies were used to monitor the environmental effect of host and non-host crop on rhizobial populations. The proportion of soil rhizobia able to nodulate pea differed between a competitive and poorly competitive isolate. Numbers of rhizobia declined over time in non-rhizosphere soil and increased in the presence of host plant. Both isolates maintained or increased soil populations from the initial level in the presence of certain non-host plants. The proportion of rhizobia available to nodulate a pea root increased more for the poorly competitive isolate in the presence of host and non-host root systems but did not reach the level of the highly competitive isolate

Savage embraces: James Purdy, melodrama, and the narration of identity

In Savage Embraces: James Purdy, Melodrama, and the Narration of Identity, Looi van Kessel explores the ways in which the early works of the American author James Purdy undermine the notion of a stable and true identity. Writing in the 1950s and 60s, a time in which identity politics enjoyed increased purchase in the United States, Purdy imagines characters who feel the urge to act out their sexual desires without having to conform to oppressive identity categories. In so doing, Purdy is searching for a language that shows how identity is produced through narration. To tease out this language, Looi approaches Purdy’s writing through the mode of melodrama—a mode that focuses on the aesthetic dramatization of tensions in the plot—while also bringing his work in conversation with current queer thinking. Ultimately, this dissertation attempts to bring the disparate fields of narrative theory and queer theory in a meaningful relation with one another.Modern and Contemporary Studie

Illegible desire: James Purdy's resistance to sexual identity

Modern and Contemporary Studie

Effect of wheat distillers dried grains with solubles or sugar beet pulp on prevalence of Salmonella enterica Typhimurium in weaned pigs

Salmonella enterica Typhimurium (ST) is of concern in the swine industry with relevance for animal health and consumer safety. Nutritional strategies might help to reduce ST infection and transmission. This study examined the potential of wheat (Triticum aestivum) distillers dried grains with solubles (DDGS) and sugar beet (Beta vulgaris) pulp (SBP) to alter intestinal microbial communities and ST shedding using a Trojan model. Weaned pigs (n = 105; 28.5 ± 3.5 d of age) were separated into 3 treatment groups (7 pigs/pen) and fed a wheat-based control diet or the control diet formulated with 15% wheat DDGS or 6% SBP inclusion. Following 12 d of diet adaptation, 2 pigs/pen were inoculated with 2 x 109 cfu ST, resistant to novobiocin and nalidixic acid. Fecal swabs were taken from infected pigs and pen-mates (contact pigs) for 9 d following challenge, enriched in nutrient broth for 24 h, and plated on selective media to determine prevalence of ST. The ranges of prevalence of ST in feces were from 90 to 100% in challenged pigs and 74 to 78% in contact pigs. No influence of treatment on rectal temperature and prevalence of ST in contact pigs were observed. Fifteen contact pigs were euthanized per treatment group on 9 and 10 d postchallenge to enumerate in intestinal contents (ileum, cecum, and proximal colon), Lactobacillus spp., Enterobacteriaceae, and Clostridium clusters I, VI, and XVIa by quantitative PCR (qPCR) and to determine ST prevalence by selective culture. No significant effects of diet were observed with respect to ST prevalence in feces, ileum, cecum, colon, and lymph nodes of contact pigs. Compared with the control diet, DGGS and SBP diets showed a trend towards increased (P < 0.1) number of Lactobacillus species in the cecum and colon. Although both wheat DGGS and SBP tended to increase the Lactobacillus spp. neither of the feed ingredients affected ST prevalence

CRISPR/Cas9-induced (CTG⋅CAG)n repeat instability in the myotonic dystrophy type 1 locus: implications for therapeutic genome editing

Myotonic dystrophy type 1 (DM1) is caused by (CTG⋅CAG)n-repeat expansion within the DMPK gene and thought to be mediated by a toxic RNA gain of function. Current attempts to develop therapy for this disease mainly aim at destroying or blocking abnormal properties of mutant DMPK (CUG)n RNA. Here, we explored a DNA-directed strategy and demonstrate that single clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-cleavage in either its 5′ or 3′ unique flank promotes uncontrollable deletion of large segments from the expanded trinucleotide repeat, rather than formation of short indels usually seen after double-strand break repair. Complete and precise excision of the repeat tract from normal and large expanded DMPK alleles in myoblasts from unaffected individuals, DM1 patients, and a DM1 mouse model could be achieved at high frequency by dual CRISPR/Cas9-cleavage at either side of the (CTG⋅CAG)n sequence. Importantly, removal of the repeat appeared to have no detrimental effects on the expression of genes in the DM1 locus. Moreover, myogenic capacity, nucleocytoplasmic distribution, and abnormal RNP-binding behavior of transcripts from the edited DMPK gene were normalized. Dual sgRNA-guided excision of the (CTG⋅CAG)n tract by CRISPR/Cas9 technology is applicable for developing isogenic cell lines for research and may provide new therapeutic opportunities for patients with DM1

Editorial Introduction

Modern and Contemporary Studie

Gebieden met bijzondere ecologische waarden op het Nederlands Continentaal Plat

Coproductie van Rijkswaterstaat RIKZ en Alterra Texel, met medewerking van RIVO en NIOZ, in opdracht van Verkeer en Waterstaat (directie Water) en LNV (directie Natuur). In navolging van wat Nota Ruimte aangeeft, komen de territoriale wateren van Noordzee in aanmerking voor beschermd gebied. Dit onderzoek zal meegenomen worden in het medio 2005 uit te brengen Integraal Beheerplan Noordzee 2015. Dit onderzoek behandelt namelijk de nadere begrenzing van het aan te wijzen gebied, aan de hand van internationaal in EU en OSPAR kader overeengekomen criteri

Metagenomic recovery of two distinct comammox Nitrospira from the terrestrial subsurface

Contains fulltext : 205810pub.pdf (publisher's version ) (Open Access)Summary The recently discovered comammox process encompasses both nitrification steps, the aerobic oxidation of ammonia and nitrite, in a single organism. All known comammox bacteria are affiliated with Nitrospira sublineage II and can be grouped into two distinct clades, referred to as A and B, based on ammonia monooxygenase phylogeny. In this study, we report high-quality draft genomes of two novel comammox Nitrospira from the terrestrial subsurface, representing one clade A and one clade B comammox organism. The two metagenome-assembled genomes were compared with other representatives of Nitrospira sublineage II, including both canonical and comammox Nitrospira. Phylogenomic analyses confirmed the affiliation of the two novel Nitrospira with comammox clades A and B respectively. Based on phylogenetic distance and pairwise average nucleotide identity values, both comammox Nitrospira were classified as novel species. Genomic comparison revealed high conservation of key metabolic features in sublineage II Nitrospira, including respiratory complexes I?V and the machineries for nitrite oxidation and carbon fixation via the reductive tricarboxylic acid cycle. In addition, the presence of the enzymatic repertoire for formate and hydrogen oxidation in the Rifle clades A and B comammox genomes, respectively, suggest a broader distribution of these metabolic features than previously anticipated.11 p