Weak forms of unconditionality of bases in greedy approximation

We study a new class of bases, weaker than quasi-greedy bases, which retain their unconditionality properties and can provide the same optimality for the thresholding greedy algorithm. We measure how far these bases are from being unconditional and use this concept to give a new characterization of nearly unconditional bases.F. Albiac acknowledges the support of the Spanish Ministry for Science and Innovation under Grant PID2019-107701GB-I00 for Operators, lattices, and structure of Banach spaces. F. Albiac and J. L. Ansorena acknowledge the support of the Spanish Ministry for Science, Innovation, and Universities under Grant PGC2018-095366-B-I00 for Analisis Vectorial, Multilineal y Aproximacion. M. Berasategui and S. Lassalle were supported by ANPCyT PICT-2018-04104 and CONICET PIP 1609. P. Berna by Grant PID2019-105599GB-I00 (Agencia Estatal de Investigacion, Spain) and 20906/PI/18 from Fundacion Seneca (Region de Murcia, Spain). S. Lassalle was also supported in part by CONICET PIP 0483 and PAI UdeSA 2020-2021

Carotenoids from persimmon juice processing

[EN] The aim of this study was the use and revalorization of two persimmon by-products A and B generated in the juice production process. The by-product B resulting from a pectinase enzymatic treatment of peels and pulp to optimize juice extraction was especially suitable for recovery of valuable bioactive carotenoids. The extraction solvents and solvent combinations used were: ethanol, acetone, ethanol/acetone (50:50 v/v) and ethanol/ acetone/hexane (25:25:50 v/v/v). HPLC-DAD analysis detected and identified a total of nine individual carotenoids namely violaxanthin, neoxanthin, antheraxanthin, lutein, zeaxanthin, ß-cryptoxanthin 5,6-epoxide, ß-cryptoxanthin, ¿-carotene, and ß-carotene. ß-cryptoxanthin and ß-carotene represented 49.2% and 13.2% of the total carotenoid content (TCC) in the acetone extract from by-product B. TCC contributed greatly to antioxidant activity of acetone extract derived from this by-product. Pectinase enzymatic treatment of persimmon peels and pulp followed by absolute acetone extraction of carotenoids could be an efficient method to obtain a rich extract in these compounds that could be used as nutraceutical ingredient.This study was supported by Ministerio de Ciencia, Innovacion y Universidades through the funded project 'Simbiosis industrial en el aprovechamiento integral del caqui (Diospyros kaki); Ejemplo de bioeconomia' (CTM2017-88978-R). Sara Gea-Botella thanks the Agencia Estatal de Investigacion la Ayuda para la Formacion de Doctores en Empresas "Doctorados Industriales" (DI-16-08465) through the R+D+i project entitled 'Evaluacion in vitro e in vivo de un extracto procedente de subproductos de la industrializacion del caqui'. The authors wish to thank Mitra Sol Technologies S.L. the given technical assistance.Gea-Botella, S.; Agulló, L.; Martí, N.; Martínez-Madrid, M.; Lizama Abad, V.; Martín-Bermudo, F.; Berná, G.... (2021). Carotenoids from persimmon juice processing. Food Research International. 141:1-8. https://doi.org/10.1016/j.foodres.2020.109882S1814

High-resolution seasonal and decadal inventory of anthropogenic gas-phase and particle emissions for Argentina

This work presents the integration of a gas-phase and particulate atmospheric emission inventory (AEI) for Argentina in high spatial resolution (0.025×0.025; approx. 2.5km×2.5 km) considering monthly variability from 1995 to 2020. The new inventory, called GEAA-AEIv3.0M, includes the following activities: Energy production, fugitive emissions from oil and gas production, industrial fuel consumption and production, transport (road, maritime, and air), agriculture, livestock production, manufacturing, residential, commercial, and biomass and agricultural waste burning. The following species, grouped by atmospheric reactivity, are considered: (i) greenhouse gases (GHGs)-CO2, CH4, and N2O; (ii) ozone precursors-CO, NOx (NO+NO2), and non-methane volatile organic compounds (NMVOCs); (iii) acidifying gases-NH3 and SO2; and (iv) particulate matter (PM)-PM10, PM2.5, total suspended particles (TSPs), and black carbon (BC). The main objective of the GEAA-AEIv3.0M high-resolution emission inventory is to provide temporally resolved emission maps to support air quality and climate modeling oriented to evaluate pollutant mitigation strategies by local governments. This is of major concern, especially in countries where air quality monitoring networks are scarce, and the development of regional and seasonal emissions inventories would result in remarkable improvements in the time and space chemical prediction achieved by air quality models. Despite distinguishing among different sectoral and activity databases as well as introducing a novel spatial distribution approach based on census radii, our high-resolution GEAA-AEIv3.0M shows equivalent national-wide total emissions compared to the Third National Communication of Argentina (TNCA), which compiles annual GHG emissions from 1990 through 2014 (agreement within ±7.5%). However, the GEAA-AEIv3.0M includes acidifying gases and PM species not considered in TNCA. Temporal comparisons were also performed against two international databases: Community Emissions Data System (CEDS) and EDGAR HTAPv5.0 for several pollutants; for EDGAR it also includes a spatial comparison. The agreement was acceptable within less than 30% for most of the pollutants and activities, although a >90% discrepancy was obtained for methane from fuel production and fugitive emissions and >120% for biomass burning. Finally, the updated seasonal series clearly showed the pollution reduction due to the COVID-19 lockdown during the first quarter of year 2020 with respect to same months in previous years. Through an open-access data repository, we present the GEAA-AEIv3.0M inventory as the largest and more detailed spatial resolution dataset for the Argentine Republic, which includes monthly gridded emissions for 12 species and 15 stors between 1995 and 2020.Fil: Puliafito, Salvador Enrique. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bolanõ Ortiz, Tomás R.. Universidad Tecnológica Nacional; Argentina. Universidad Técnica Federico Santa María; ChileFil: Fernandez, Rafael Pedro. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Berná, Lucas L.. Universidad Tecnológica Nacional; Argentina. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; ArgentinaFil: Pascual Flores, Romina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Tecnológica Nacional; ArgentinaFil: Urquiza, Josefina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Lopez Noreña, Ana Isabel. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo; ArgentinaFil: Tames, María Florencia. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentin

Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype

In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.Fil: Colombatti Olivieri, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Fresia, P.. Instituto Pasteur de Montevideo; UruguayFil: Graña, M.. Instituto Pasteur de Montevideo; UruguayFil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Nagel, Ariel Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Berná, L.. Instituto Pasteur de Montevideo; UruguayFil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentin

XTEND: Extending the depth of field in cryo soft X-ray tomography

We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.This work was partially supported by MINECO grants BFU2014-54181 to JLC and AIC-A-2011-0638, BIO2013-44647-R and BIO2016-76400-R to JMC, Madrid. Regional government grants S2013/MIT-2850 to JLC and S2010/BMD-2305 to JMC, National Science Foundation grant DMS-1114901 to GTH, the European Union through BioStruct-X Project 283570 and Horizon 2020 through grant iNEXT (INFRAIA-1-2014-2015, Proposal: 653706).S

Genome-wide diversity and differentiation in New World populations of the human malaria parasite Plasmodium vivax.

BACKGROUND: The Americas were the last continent colonized by humans carrying malaria parasites. Plasmodium falciparum from the New World shows very little genetic diversity and greater linkage disequilibrium, compared with its African counterparts, and is clearly subdivided into local, highly divergent populations. However, limited available data have revealed extensive genetic diversity in American populations of another major human malaria parasite, P. vivax. METHODS: We used an improved sample preparation strategy and next-generation sequencing to characterize 9 high-quality P. vivax genome sequences from northwestern Brazil. These new data were compared with publicly available sequences from recently sampled clinical P. vivax isolates from Brazil (BRA, total n = 11 sequences), Peru (PER, n = 23), Colombia (COL, n = 31), and Mexico (MEX, n = 19). PRINCIPAL FINDINGS/CONCLUSIONS: We found that New World populations of P. vivax are as diverse (nucleotide diversity π between 5.2 × 10-4 and 6.2 × 10-4) as P. vivax populations from Southeast Asia, where malaria transmission is substantially more intense. They display several non-synonymous nucleotide substitutions (some of them previously undescribed) in genes known or suspected to be involved in antimalarial drug resistance, such as dhfr, dhps, mdr1, mrp1, and mrp-2, but not in the chloroquine resistance transporter ortholog (crt-o) gene. Moreover, P. vivax in the Americas is much less geographically substructured than local P. falciparum populations, with relatively little between-population genome-wide differentiation (pairwise FST values ranging between 0.025 and 0.092). Finally, P. vivax populations show a rapid decline in linkage disequilibrium with increasing distance between pairs of polymorphic sites, consistent with very frequent outcrossing. We hypothesize that the high diversity of present-day P. vivax lineages in the Americas originated from successive migratory waves and subsequent admixture between parasite lineages from geographically diverse sites. Further genome-wide analyses are required to test the demographic scenario suggested by our data