Folding of VemP into translation-arresting secondary structure is driven by the ribosome exit tunnel

The ribosome is a fundamental biomolecular complex that synthesizes proteins in cells. Nascent proteins emerge from the ribosome through a tunnel, where they may interact with the tunnel walls or small molecules such as antibiotics. These interactions can cause translational arrest with notable physiological consequences. Here, we studied the arrest caused by the regulatory peptide VemP, which is known to form alpha-helices inside the ribosome tunnel near the peptidyl transferase center under specific conditions. We used all-atom molecular dynamics simulations of the entire ribosome and circular dichroism spectroscopy to study the driving forces of helix formation and how VemP causes the translational arrest. To that aim, we compared VemP dynamics in the ribosome tunnel with its dynamics in solution. We show that the VemP peptide has a low helical propensity in water and that the propensity is higher in mixtures of water and trifluorethanol. We propose that helix formation within the ribosome is driven by the interactions of VemP with the tunnel and that a part of VemP acts as an anchor. This anchor might slow down VemP progression through the tunnel enabling alpha-helix formation, which causes the elongation arrest

Magnetohydrostatic atmospheres

We show that the atmospheric and magnetic height variations are coupled in general MHS equilibria with gravity when isolated thin non-force-free flux tubes are present. In gas-dominated environments, as in stellar photospheres, flux tubes must expand rapidly with height to maintain pressure balance with the cool surroundings. But in magnetically dominated environments, as in stellar coronae, the large-scale background magnetic field determines the average spreading of embedded flux tubes, and rigidly held flux tubes {\it require} a specific surrounding atmosphere with a unique temperature profile for equilibrium. The solar static equilibrium atmosphere exhibits correct transition-region properties and the accepted base coronal temperature for the sun's main magnetic spherical harmonic. Steady flows contribute to the overall pressure, so equilibria with accelerated wind outflows are possible as well. Flux tubes reflect a mathematical degeneracy in the form of non-force-free fields, which leads to coupling in general equilibrium conditions. The equilibrium state characterizes the system average in usual circumstances and dynamics tend to maintain the MHS atmosphere. Outflows are produced everywhere external to rigidly held flux tubes that refill a depleted or cool atmosphere to the equilibrium gas profile, heating the gas compressively.Comment: 12 pages, 5 figures, accepted by A&

A Contemporary View of Coronal Heating

Determining the heating mechanism (or mechanisms) that causes the outer atmosphere of the Sun, and many other stars, to reach temperatures orders of magnitude higher than their surface temperatures has long been a key problem. For decades the problem has been known as the coronal heating problem, but it is now clear that `coronal heating' cannot be treated or explained in isolation and that the heating of the whole solar atmosphere must be studied as a highly coupled system. The magnetic field of the star is known to play a key role, but, despite significant advancements in solar telescopes, computing power and much greater understanding of theoretical mechanisms, the question of which mechanism or mechanisms are the dominant supplier of energy to the chromosphere and corona is still open. Following substantial recent progress, we consider the most likely contenders and discuss the key factors that have made, and still make, determining the actual (coronal) heating mechanism (or mechanisms) so difficult

Le Service Européen pour l’action Extérieure á l’heure de son épreuve : Une contribution tenforcée de l’UE au maintien de la paix ?

Cet article a pour objet de s’intéresser à l’impact que la mise en place d’un nouvel organe comme le Service Européen pour l’Action Extérieure, extrêmement original et innovateur du point de vue institutionnel, pourrait avoir à court et moyen terme sur la capacité de l’UE pour décoller défnitivement en tant qu’acteur majeur et partenaire vraiment crédible dans le domaine du maintien de la paix. Le moment semble opportun pour le faire dans la mesure où, après quelques quatre ans d’existence et une fois surmontés un certain nombre de teething problems, un processus de réflexion sur les faiblesses et potentialités du SEAE est en cours au sein du système institutionnel de l’UE en vue d’une éventuelle révision de sa Décision de base.En este trabajo se propone un análisis del impacto que la creación y puesta en funcionamiento de un nuevo órgano tan original e innovador desde el punto de vista institucional como el Servicio Europeo de Acción Exterior podría ejercer, a corto y medio plazo, sobre la capacidad de la UE para afanzarse como actor realmente signifcativo y socio internacional creíble en el campo del mantenimiento de la paz. El momento parece oportuno para ello en la medida en que, tras cuatro años de existencia y una vez superado un buen número de difcultades iniciales, se ha abierto un proceso de reflexión sobre las defciencias y potencialidades del SEAE que podría conducir a una revisión de su Decisión fundacional.This paper aims to analyse the impact that the setup and functioning of a new body, as original and innovative from an institutional point of view as the European External Action Service, could have in the consolidation of the EU’s position as a signifcant and reliable international partner in peacekeeping, both in the short and medium terms. This timing appears appropriate to do so insofar as, after four years of existence and having overcome a signifcant number of teething problems, a reflection process on the weaknesses and potentialities of the EEAS is currently in place and could eventually lead to a review of its founding Decision

High Energy Properties of X-ray Sources observed with BeppoSAX

We report on highlight results on celestial sources observed in the high energy band (>20 keV) with BeppoSAX. In particular we review the spectral properties of sources that belong to different classes of objects, i.e. stellar coronae (Algol), supernova remnants (Cas A), low mass X-ray binaries (Cygnus X-2 and the X-ray burster GS1826-238), black hole candidates (Cygnus X-1) and Active Galactic Nuclei (Mkn 3). We detect, for the first time, the broad-band spectrum of a stellar corona up to 100 keV; for Cas A we report upper limits to the ^44Ti line intensities that are lower than those available to date; for Cyg X-2 we report the evidence of a high energy component; we report a clear detection of a broad Fe K line feature from Cygnus X-1 in soft state and during its transition to hard state; Mkn 3 is one of several Seyfert 2 galaxies detected with BeppoSAX at high energies, for which Compton scattering process is important.Comment: To appear in the Proceedings of the 1997 Conference on 'The Active X-ray Sky: Results from BeppoSAX and Rossi-XTE' eds. L. Scarsi, F. Fiore and P. Giomm

Coronal Temperature Diagnostic Capability of the Hinode/X-Ray Telescope Based on Self-Consistent Calibration

The X-Ray Telescope (XRT) onboard the Hinode satellite is an X-ray imager that observes the solar corona with unprecedentedly high angular resolution (consistent with its 1" pixel size). XRT has nine X-ray analysis filters with different temperature responses. One of the most significant scientific features of this telescope is its capability of diagnosing coronal temperatures from less than 1 MK to more than 10 MK, which has never been accomplished before. To make full use of this capability, accurate calibration of the coronal temperature response of XRT is indispensable and is presented in this article. The effect of on-orbit contamination is also taken into account in the calibration. On the basis of our calibration results, we review the coronal-temperature-diagnostic capability of XRT

Dynamics of Coronal Bright Points as seen by Sun Watcher using Active Pixel System detector and Image Processing (SWAP), Atmospheric Imaging Assembly AIA), and Helioseismic and Magnetic Imager (HMI)

The \textit{Sun Watcher using Active Pixel system detector and Image Processing}(SWAP) on board the \textit{PRoject for OnBoard Autonomy\todash 2} (PROBA\todash 2) spacecraft provides images of the solar corona in EUV channel centered at 174 \AA. These data, together with \textit{Atmospheric Imaging Assembly} (AIA) and the \textit{Helioseismic and Magnetic Imager} (HMI) on board \textit{Solar Dynamics Observatory} (SDO), are used to study the dynamics of coronal bright points. The evolution of the magnetic polarities and associated changes in morphology are studied using magnetograms and multi-wavelength imaging. The morphology of the bright points seen in low-resolution SWAP images and high-resolution AIA images show different structures, whereas the intensity variations with time show similar trends in both SWAP 174 and AIA 171 channels. We observe that bright points are seen in EUV channels corresponding to a magnetic-flux of the order of $10^{18}$ Mx. We find that there exists a good correlation between total emission from the bright point in several UV\todash EUV channels and total unsigned photospheric magnetic flux above certain thresholds. The bright points also show periodic brightenings and we have attempted to find the oscillation periods in bright points and their connection to magnetic flux changes. The observed periods are generally long (10\todash 25 minutes) and there is an indication that the intensity oscillations may be generated by repeated magnetic reconnection

Cross-correlation of the 2XMMi catalogue with Data Release 7 of the Sloan Digital Sky Survey

The Survey Science Centre of the XMM-Newton satellite released the first incremental version of the 2XMM catalogue in August 2008 . With more than 220,000 X-ray sources, the 2XMMi was at that time the largest catalogue of X-ray sources ever published and thus constitutes an unprecedented resource for studying the high-energy properties of various classes of X-ray emitters such as AGN and stars. The advent of the 7th release of the Sloan Digital Sky Survey offers the opportunity to cross-match two major surveys and extend the spectral energy distribution of many 2XMMi sources towards the optical bands. We here present a cross-matching algorithm based on the classical likelihood ratio estimator. The method developed has the advantage of providing true probabilities of identifications without resorting to Monte-Carlo simulations. Over 30,000 2XMMi sources have SDSS counterparts with individual probabilities of identification higher than 90%. Using spectroscopic identifications from the SDSS DR7 catalogue supplemented by extraction from other catalogues, we build an identified sample from which the way the various classes of X-ray emitters gather in the multi dimensional parameter space can be analysed. We investigate two scientific use cases. In the first example we show how these multi-wavelength data can be used to search for new QSO2s. Although no specific range of observed properties allows us to identify Compton Thick QSO2s, we show that the prospects are much better for Compton Thin AGN2 and discuss several possible multi-parameter selection strategies. In a second example, we confirm the hardening of the mean X-ray spectrum with increasing X-ray luminosity on a sample of over 500 X-ray active stars and reveal that on average X-ray active M stars display bluer $g-r$ colour indexes than less active ones (abridged).Comment: Accepted for publication in A&A. The corresponding fits file can be downloaded from the XCat-DB home page (http://xcatdb.u-strasbg.fr/) (tools and data). The file also contains line information for all SDSS spectroscopic entries matching a 2XMM source. Results from the cross-correlation with the 2XMM DR3 are also available at the same location. 22 pages and 14 figure

Automated Coronal Hole Detection using Local Intensity Thresholding Techniques

We identify coronal holes using a histogram-based intensity thresholding technique and compare their properties to fast solar wind streams at three different points in the heliosphere. The thresholding technique was tested on EUV and X-ray images obtained using instruments onboard STEREO, SOHO and Hinode. The full-disk images were transformed into Lambert equal-area projection maps and partitioned into a series of overlapping sub-images from which local histograms were extracted. The histograms were used to determine the threshold for the low intensity regions, which were then classified as coronal holes or filaments using magnetograms from the SOHO/MDI. For all three instruments, the local thresholding algorithm was found to successfully determine coronal hole boundaries in a consistent manner. Coronal hole properties extracted using the segmentation algorithm were then compared with in situ measurements of the solar wind at 1 AU from ACE and STEREO. Our results indicate that flux tubes rooted in coronal holes expand super-radially within 1 AU and that larger (smaller) coronal holes result in longer (shorter) duration high-speed solar wind streams