Modelo de receptor de antígeno quimérico en la línea celular Jurkat

En este TFG se explicarán los pasos necesarios para conseguir la expresión de un receptor de antígeno quimérico (CAR) en la línea celular Jurkat, así como la generación de un doble knock-out en los genes TRAC y B2M (que codifican respectivamente para el receptor de antígeno del lifocito T, TCR y para la beta2-microblobulina, proteína que forma parte del complejo mayor de histocompatibilidad de clase I, MHC-I). Esto se llevará a cabo mediante una herramienta de edición génica, variante del sistema CRISPR/Cas9, que se basa en el vanguardista método de unión de extremos independiente de homología (HITI) y que permitirá seleccionar una diana para realizar el corte en la doble cadena de DNA (generando el knock-out en el gen predeterminado), posibilitando la integración del material genético donado por los vectores en el lugar de la escisión. La generación de células modificadas genéticamente, más concretamente linfocitos T, abre numerosas posibilidades para ofrecer terapias curativas en pacientes afectados por cánceres de diversa índole, ofreciendo además especificidad gracias al dominio extramembrana del CAR.Grado en Medicin

Hábitos relacionados con la relajación y la atención plena (mindfulness) en estudiantes de secundaria

Podeu consultar el document complet a: http://hdl.handle.net/2445/58585El objetivo de este trabajo es conocer los hábitos personales, familiares y escolares relacionados con la relajación y el mindfulness y averiguar su incidencia tanto en el clima de clase como en el rendimiento académico. Para ello, se utilizó el CBCC (Cuestionario Breve del Clima de Clase de López-González y Bisquerra, 2013, la media de calificaciones globales de final de curso y dos instrumentos para mediar los hábitos: un diario y una escala diseñada para la ocasión (EHRE: Escala de Hábitos de Relajación Escolar). El estudio se llevó a cabo con una muestra n = 490 de alumnos de ESO y bachillerato de un instituto. Los resultados muestran que los hábitos personales y familiares predicen en el clima de clase y el rendimiento académico. Existen diferencias significativas por sexo y por nivel académico

Fractionation and fluxes of metals and radionuclides during the recycling process of phosphogypsum wastes applied to mineral CO2 sequestration

The industry of phosphoric acid produces a calcium-rich by-product known as phosphogypsum, which is usually stored in large stacks of millions of tons. Up to now, no commercial application has been widely implemented for its reuse because of the significant presence of potentially toxic contaminants. This work confirmed that up to 96% of the calcium of phosphogypsum could be recycled for CO2 mineral sequestration by a simple two-step process: alkaline dissolution and aqueous carbonation, under ambient pressure and temperature. This CO2 sequestration process based on recycling phosphogypsum wastes would help to mitigate greenhouse gasses emissions. Yet this work goes beyond the validation of the sequestration procedure; it tracks the contaminants, such as trace metals or radionuclides, during the recycling process in the phosphogypsum. Thus, most of the contaminants were transferred from raw phosphogypsum to portlandite, obtained by dissolution of the phosphogypsum in soda, and from portlandite to calcite during aqueous carbonation. These findings provide valuable information for managing phosphogypsum wastes and designing potential technological applications of the by-products of this environmentally-friendly proposal.Junta de Andalucía P10-RNM-6300, P12- RNM-226

Design of asymptotically optimal improper constellations with hexagonal packing

This paper addresses the problem of designing asymptotically optimal improper constellations with a given circularity coefficient (correlation coefficient between the constellation and its complex conjugate). The designed constellations are optimal in the sense that, at high signal-to-noise-ratio (SNR) and for a large number of symbols, yield the lowest probability of error under an average power constraint for additive white Gaussian noise channels. As the number of symbols grows, the optimal constellation is the intersection of the hexagonal lattice with an ellipse whose eccentricity determines the circularity coefficient. Based on this asymptotic result, we propose an algorithm to design finite improper constellations. The proposed constellations provide significant SNR gains with respect to previous improper designs, which were generated through a widely linear transformation of a standard M-ary quadrature amplitude modulation constellation. As an application example, we study the use of these improper constellations by a secondary user in an underlay cognitive radio network.The work of Jesús A. López-Fernández and R. G. Ayestarán was partly supported by the Ministerio de Ciencia, Innovación y Universidades under project TEC2017-86619-R (ARTEINE), and by the Gobierno del Principado de Asturias under project GRUPIN-IDI2018-000191. The work of I. Santamaria was partly supported by the Ministerio de Economía y Competitividad (MINECO) of Spain, and AEI/FEDER funds of the E.U., under grant TEC2016-75067-C4-4-R (CARMEN) and TEC2015-69648-REDC (Red COMONSENS). The work of C. Lameiro was supported by the German Research Foundation (DFG) under grant LA 4107/1-1

Immediate loading and immediate restoration in 105 expanded-platform implants via the Diem System after a 16-month follow-up period

Objective: The aim of this study was to report on our experience and outcomes with Certain Prevail Implants and immediate loading via the Diem System after a 16-month follow-up period. Materials and Methods: Over a 16- month period, 105 (14 maxilla, 91 mandible) expanded-platform implants were placed in 18 patients (15 females, 3 males; 55.97 ±7.25 SD years). Resonance frecuency analysis (RFA) was measured on the day of placement and at 3, 12 and 16 months. All prostheses were screw mounted on IOL DIEM standard abutments. The follow-up time varied between 3 up to 16 months. Results: One implant (0.9%) failed during final prothetic placement (3 months). The RFA (ISQ) measurements at 16 months evalution for 4 mm- and 5 mm diameter implants were: 76.13 ± 5.0 SD and 76 ±7.7 SD respectively. The RFA data related to mandible and maxilla, the day 0 mean was 76.47 ± 4.75 SD and 70.13 ± 1.35 SD, at 3 months 70.53 ± 4.0 SD and 66.07 ±2.9 SD, at 12 months 68.23± 3.6 SD and 66.40 ±2.7 SD and 16 months 68.23± 3.6 SD and 66.60 ± 2.5 SD respectively. The crestal bone resorption was 0.6 mm at 16 months in platform switching implants.Conclusions: The cumulative implant survival rate during the follow-up period was 99.1% Immediate loading on IOL Diem abutments is a reliable and effective technique for edentulous patients in the maxilla and mandible

Construction of a system of flow and temperature instrumentation on a porcine farm in the municipality of Marsella, Risaralda

We present a local monitoring system of temperature and caudal in a pig farm. The method consists of designing an instrumentation and measurement system, this uses a wireless sensor network (WSN) based on the ZigBee standard. The WSN sends the gathered data to a server that stores the information in a database with the purpose of consulting (local queries) at any time the data that have been measured by the electronic devices. The preliminary results show that the data we can be used to infer behavior of the variables under study, besides the prototype is scalable, efficient, that makes it easily adaptable to any pig farm.We present a local monitoring system of temperature and caudal in a pig farm. The method consists of designing an instrumentation and measurement system, this uses a wireless sensor network (WSN) based on the ZigBee standard. The WSN sends the gathered data to a server that stores the information in a database with the purpose of consulting (local queries) at any time the data that have been measured by the electronic devices. The preliminary results show that the data we can be used to infer behavior of the variables under study, besides the prototype is scalable, efficient, that makes it easily adaptable to any pig farm

Construction of a system of flow and temperature instrumentation on a porcine farm in the municipality of Marsella, Risaralda

We present a local monitoring system of temperature and caudal in a pig farm. The method consists of designing an instrumentation and measurement system, this uses a wireless sensor network (WSN) based on the ZigBee standard. The WSN sends the gathered data to a server that stores the information in a database with the purpose of consulting (local queries) at any time the data that have been measured by the electronic devices. The preliminary results show that the data we can be used to infer behavior of the variables under study, besides the prototype is scalable, efficient, that makes it easily adaptable to any pig farm.We present a local monitoring system of temperature and caudal in a pig farm. The method consists of designing an instrumentation and measurement system, this uses a wireless sensor network (WSN) based on the ZigBee standard. The WSN sends the gathered data to a server that stores the information in a database with the purpose of consulting (local queries) at any time the data that have been measured by the electronic devices. The preliminary results show that the data we can be used to infer behavior of the variables under study, besides the prototype is scalable, efficient, that makes it easily adaptable to any pig farm

Construction of a system of flow and temperature instrumentation on a porcine farm in the municipality of Marsella, Risaralda

We present a local monitoring system of temperature and caudal in a pig farm. The method consists of designing an instrumentation and measurement system, this uses a wireless sensor network (WSN) based on the ZigBee standard. The WSN sends the gathered data to a server that stores the information in a database with the purpose of consulting (local queries) at any time the data that have been measured by the electronic devices. The preliminary results show that the data we can be used to infer behavior of the variables under study, besides the prototype is scalable, efficient, that makes it easily adaptable to any pig farm.We present a local monitoring system of temperature and caudal in a pig farm. The method consists of designing an instrumentation and measurement system, this uses a wireless sensor network (WSN) based on the ZigBee standard. The WSN sends the gathered data to a server that stores the information in a database with the purpose of consulting (local queries) at any time the data that have been measured by the electronic devices. The preliminary results show that the data we can be used to infer behavior of the variables under study, besides the prototype is scalable, efficient, that makes it easily adaptable to any pig farm

Changes in biomarkers of redox status in serum and saliva of dogs with hypothyroidism

Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on a dog's life quality. This study aims to evaluate changes in the redox status in canine hypothyroidism. For this purpose, a comprehensive panel of antioxidants and oxidants biomarkers were measured in serum and saliva of 23 dogs with hypothyroidism, 21 dogs with non-thyroidal illness, and 16 healthy dogs. Among the antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol, paraoxonase type 1 (PON-1) and glutathione peroxidase (GPx) were determined in serum and CUPRAC, ferric reducing ability of saliva (FRAS) and TEAC in saliva. The oxidant biomarkers included were total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP), and thiobarbituric acid reactive substances (TBARS) in serum and AOPP and TBARS in saliva. Results showed a significantly higher TEAC, PON-1, GPx, TOS, POX-Act, and d-ROMs, and a significantly lower AOPP in serum of dogs with hypothyroidism. Meanwhile, significantly lower FRAS and AOPP were observed in saliva of dogs with hypothyroidism. Once salivary concentrations were corrected based on their total protein concentrations, the only analyte showing significant changes was TBARS which was significantly higher in dogs with hypothyroidism. Our results show that dogs with hypothyroidism present alterations in the redox status in both serum and saliva. This study should be considered a preliminary study and further research addressing these changes should be made using larger populations. The online version contains supplementary material available at 10.1186/s12917-023-03586-4

Seasonal and interannual variability of dissolved oxygen around the Balearic Islands from hydrographic data

Oceanographic data obtained between 2001 and 2011 by the Spanish Institute of Oceanography (IEO, Spain) have been used to characterise the spatial distribution and the temporal variability of the dissolvedoxygen around the Balearic Islands (Mediterranean Sea). The study area includes most of the Western Mediterranean Sea, from the Alboran Sea to Cape Creus,atthe border between France and Spain. Dissolved Oxygen (DO) at thewatersurface is found to be in a state of equilibrium exchange with the atmosphere. In the spring and summer a subsurface oxygen supersaturation is observed due to the biological activity, above the subsurface fluorescence maximum. Minimum observed values of dissolved oxygen are related to theLevantine Intermediate Waters (LIW). An unusual minimum of dissolved oxygen concentrations were also recorded in the Alboran Sea Oxygen Minimum Zone. The Western Mediterranean Deep Waters (WMDW) and the Western Intermediate Waters (WIW) show higher values of dissolved oxygenthanthe Levantine Intermediate Waters due to their more recent formation. Using these dissolved oxygen concentrations it is possible to showthat the Western Intermediate Waters move southwards across the Ibiza Channel and the deep water circulates around the Balearic Islands. It has also been possible to characterise the seasonal evolution of the different watermassesandtheir dissolved oxygen content in a station in the Algerian sub-basin. Keywords: Ocean circulation, dissolved oxygen, water masses, Western Mediterranean Sea, Balearic SeaPost-print