Environmental History of Lago di Tovel, Trento, Italy, Revealed by Sediment Cores and 3.5kHz Seismic Mapping

Proxy data from a total of 30 sediment cores and information from a seismic survey show that the sedimentological and limnological history of Lago di Tovel (1178m a.s.l.) has been significantly influenced by slope dynamics of its mountainous catchment. The lake represents a dead-ice lake with pro-glacial deposits at the base of its sedimentary record. A prominent lake level rise in 1597/1598 that increased maximum water depth from ∼20 to 39m caused slope instabilities, leading to the deposition of mass-flow sediments with a maximum thickness of 2.5m in the northern part of the lake and less than 50cm in the southern part, resulting in a total volume of more than 113,000m3. Consequently, a rough lake bottom morphology was produced, which led to distinct differences in sedimentation rates of 0.07cmyr−1 on sills and 0.18cmyr−1 within depressions. The age of the top of the mass-flow deposits was used to validate the ages of the younger, laminated sediments, which were dated by 210Pb and 137Cs. Lithological investigations showed that the sediments below the mass-flow deposits are also laminated and that they were not bioturbated. The long-term meromixis of Lago di Tovel is therefore mainly due to a combination of its topographic setting and the 5-month period of ice cover. Both prevent effective mixing of the lake by strong winds during spring and autumn. Distinct spatial differences in sediment distribution within the lake show that it is risky to interpret proxy data from only one coring site, even if the lake is very small. This is especially true in mountainous areas, where rock falls, mass movements, and slope instabilities of a significant size may have considerable effects on lake

Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

2,5-diketo-D-gluconic acid reductase (2,5-DKG reductase) catalyses the reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG), a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid. As 2,5-DKG reductase is usually produced recombinantly, it is of interest to establish an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. In the present study, three recently described food grade variants of the Lactobacillales based expression systems pSIP (Lactobacillus plantarum) and NICE (Lactococcus lactis) were evaluated with regard to their effictiveness to produce 2,5-DKG reductase from Corynebacterium glutamicum. Our results indicate that both systems are suitable for 2,5-DKG reductase expression. Maximum production yields were obtained with Lb. plantarum/pSIP609 by pH control at 6.5. With 262 U per litre of broth, this represents the highest heterologous expression level so far reported for 2,5-DKG reductase from C. glutamicum. Accordingly, Lb. plantarum/pSIP609 might be an interesting alternative to Escherichia coli expression systems for industrial 2,5-DKG reductase production

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer

CRUK programme grant C587/A16354 and a research grant from Affitech AS.The study was supported by Cancer Research UK (CRUK) programme grant C587/A16354 and a research grant from Affitech AS

Sediment accumulation rates in subarctic lakes: Insights into age-depth modeling from 22 dated lake records from the Northwest Territories, Canada

Age-depth modeling using Bayesian statistics requires well-informed prior information about the behavior of sediment accumulation. Here we present average sediment accumulation rates (represented as deposition times, DT, in yr/cm) for lakes in an Arctic setting, and we examine the variability across space (intra- and inter-lake) and time (late Holocene). The dataset includes over 100 radiocarbon dates, primarily on bulk sediment, from 22 sediment cores obtained from 18 lakes spanning the boreal to tundra ecotone gradients in subarctic Canada. There are four to twenty-five radiocarbon dates per core, depending on the length and character of the sediment records. Deposition times were calculated at 100-year intervals from age-depth models constructed using the 'classical' age-depth modeling software Clam. Lakes in boreal settings have the most rapid accumulation (mean DT 20±10 yr/cm), whereas lakes in tundra settings accumulate at moderate (mean DT 70±10 yr/cm) to very slow rates, (>100yr/cm). Many of the age-depth models demonstrate fluctuations in accumulation that coincide with lake evolution and post-glacial climate change. Ten of our sediment cores yielded sediments as old as c. 9000cal BP (BP=years before AD 1950). From between c. 9000cal BP and c. 6000cal BP, sediment accumulation was relatively rapid (DT of 20-60yr/cm). Accumulation slowed between c. 5500 and c. 4000cal BP as vegetation expanded northward in response to warming. A short period of rapid accumulation occurred near 1200cal BP at three lakes. Our research will help inform priors in Bayesian age modeling