208 research outputs found
Ultrafast Mid-IR Laser Scalpel: Protein Signals of the Fundamental Limits to Minimally Invasive Surgery
Lasers have in principle the capability to cut at the level of a single cell, the fundamental limit to minimally invasive procedures and restructuring biological tissues. To date, this limit has not been achieved due to collateral damage on the macroscale that arises from thermal and shock wave induced collateral damage of surrounding tissue. Here, we report on a novel concept using a specifically designed Picosecond IR Laser (PIRL) that selectively energizes water molecules in the tissue to drive ablation or cutting process faster than thermal exchange of energy and shock wave propagation, without plasma formation or ionizing radiation effects. The targeted laser process imparts the least amount of energy in the remaining tissue without any of the deleterious photochemical or photothermal effects that accompanies other laser wavelengths and pulse parameters. Full thickness incisional and excisional wounds were generated in CD1 mice using the Picosecond IR Laser, a conventional surgical laser (DELight Er:YAG) or mechanical surgical tools. Transmission and scanning electron microscopy showed that the PIRL laser produced minimal tissue ablation with less damage of surrounding tissues than wounds formed using the other modalities. The width of scars formed by wounds made by the PIRL laser were half that of the scars produced using either a conventional surgical laser or a scalpel. Aniline blue staining showed higher levels of collagen in the early stage of the wounds produced using the PIRL laser, suggesting that these wounds mature faster. There were more viable cells extracted from skin using the PIRL laser, suggesting less cellular damage. β-catenin and TGF-β signalling, which are activated during the proliferative phase of wound healing, and whose level of activation correlates with the size of wounds was lower in wounds generated by the PIRL system. Wounds created with the PIRL systsem also showed a lower rate of cell proliferation. Direct comparison of wound healing responses to a conventional surgical laser, and standard mechanical instruments shows far less damage and near absence of scar formation by using PIRL laser. This new laser source appears to have achieved the long held promise of lasers in minimally invasive surgery
Tycho 2 stars with infrared excess in the MSX Point Source Catalogue
Stars of all evolutionary phases have been found to have excess infrared
emission due to the presence of circumstellar material. To identify such stars,
we have positionally correlated the infrared MSX point source catalogue and the
Tycho 2 optical catalogue. A near/mid infrared colour criteria has been
developed to select infrared excess stars. The search yielded 1938 excess
stars, over half (979) have never previously been detected by IRAS. The excess
stars were found to be young objects such as Herbig Ae/Be and Be stars, and
evolved objects such as OH/IR and carbon stars. A number of B type excess stars
were also discovered whose infrared colours could not be readily explained by
known catalogued objects.Comment: Added Comment: 16 pages, 9 figures, accepted for publication in MNRA
Lycoris -- a large-area, high resolution beam telescope
A high-resolution beam telescope is one of the most important and demanding
infrastructure components at any test beam facility. Its main purpose is to
provide reference particle tracks from the incoming test beam particles to the
test beam users, which allows measurement of the performance of the
device-under-test (DUT). \LYCORIS, a six-plane compact beam telescope with an
active area of 10\SI{10}{\square\centi\metre} (extensible to
10\SI{20}{\square\centi\metre}) was installed at the \DIITBF in 2019,
to provide a precise momentum measurement in a \SI{1}{\tesla} solenoid magnet
or to provide tracking over a large area. The overall design of \LYCORIS will
be described as well as the performance of the chosen silicon sensor. The
\SI{25}{\micro\metre} pitch micro-strip sensor used for \LYCORIS was originally
designed for the \SID detector concept for the International Linear Collider.
It adopts a second metallization layer to route signals from strips to the
bump-bonded \KPIX ASIC and uses a wire-bonded flex cable for the connection to
the DAQ and the power supply system. This arrangement eliminates the need for a
dedicated hybrid PCB. Its performance was tested for the first time in this
project. The system has been evaluated at the \DIITBF in several test-beam
campaigns and has demonstrated an average single-point resolution of
\SI{7.07}{\micro\meter}.Comment: 43 pages, 37 figure
Galactic bulge giants: probing stellar and galactic evolution I. Catalogue of Spitzer IRAC and MIPS sources
Aims: We aim at measuring mass-loss rates and the luminosities of a
statistically large sample of Galactic bulge stars at several galactocentric
radii. The sensitivity of previous infrared surveys of the bulge has been
rather limited, thus fundamental questions for late stellar evolution, such as
the stage at which substantial mass-loss begins on the red giant branch and its
dependence on fundamental stellar properties, remain unanswered. We aim at
providing evidence and answers to these questions. Methods: To this end, we
observed seven 15 times 15 arcmin^2 fields in the nuclear bulge and its
vicinity with unprecedented sensitivity using the IRAC and MIPS imaging
instruments on-board the Spitzer Space Telescope. In each of the fields, tens
of thousands of point sources were detected. Results: In the first paper based
on this data set, we present the observations, data reduction, the final
catalogue of sources, and a detailed comparison to previous mid-IR surveys of
the Galactic bulge, as well as to theoretical isochrones. We find in general
good agreement with other surveys and the isochrones, supporting the high
quality of our catalogue.Comment: 21 pages, accepted for publication in A&A. A version with
high-resolution figures, as well as the data catalogues (including cross-id
with GLIMPSE and GALCEN) and image mosaics are available at the anonymous
ftp://ftp.ster.kuleuven.be/dist/stefan/Spitzer
The RMS Survey: Mid-Infrared Observations of Candidate Massive YSOs in the Southern Hemisphere
Abridged abstract: The Red MSX Source (RMS) survey is an ongoing effort to
return a large, well-selected sample of massive young stellar objects (MYSOs)
within our Galaxy. A series of ground-based follow-up observations are being
undertaken in order to remove contaminant objects from our list of 2000
candidates, and to begin characterising these MYSOs. As a part of these
follow-up observations, high resolution (~1") mid-IR imaging aids the
identification of contaminant objects which are resolved (UCHII regions, PN) as
opposed to those which are unresolved (YSOs, evolved stars) as well as
identifying YSOs near UCHII regions and other multiple sources. We present 10.4
micron imaging observations for 346 candidate MYSOs in the RMS survey in the
Southern Hemisphere, primarily outside the region covered by the GLIMPSE
Spitzer Legacy Survey. These were obtained using TIMMI2 on the ESO 3.6m
telescope in La Silla, Chile. Our photometric accuracy is of order 0.05Jy, and
our astrometric accuracy is 0.8", which is an improvement over the nominal 2"
accuracy of the MSX PSC.Comment: 9 page paper accepted to A&A. Online data for table 2 and figure 1
will be available in the published online version of this paper via A&A. The
paper contains 7 figures and 3 table
Centre selection for clinical trials and the generalisability of results: a mixed methods study.
BACKGROUND: The rationale for centre selection in randomised controlled trials (RCTs) is often unclear but may have important implications for the generalisability of trial results. The aims of this study were to evaluate the factors which currently influence centre selection in RCTs and consider how generalisability considerations inform current and optimal practice. METHODS AND FINDINGS: Mixed methods approach consisting of a systematic review and meta-summary of centre selection criteria reported in RCT protocols funded by the UK National Institute of Health Research (NIHR) initiated between January 2005-January 2012; and an online survey on the topic of current and optimal centre selection, distributed to professionals in the 48 UK Clinical Trials Units and 10 NIHR Research Design Services. The survey design was informed by the systematic review and by two focus groups conducted with trialists at the Birmingham Centre for Clinical Trials. 129 trial protocols were included in the systematic review, with a total target sample size in excess of 317,000 participants. The meta-summary identified 53 unique centre selection criteria. 78 protocols (60%) provided at least one criterion for centre selection, but only 31 (24%) protocols explicitly acknowledged generalisability. This is consistent with the survey findings (n = 70), where less than a third of participants reported generalisability as a key driver of centre selection in current practice. This contrasts with trialists' views on optimal practice, where generalisability in terms of clinical practice, population characteristics and economic results were prime considerations for 60% (n = 42), 57% (n = 40) and 46% (n = 32) of respondents, respectively. CONCLUSIONS: Centres are rarely enrolled in RCTs with an explicit view to external validity, although trialists acknowledge that incorporating generalisability in centre selection should ideally be more prominent. There is a need to operationalize 'generalisability' and incorporate it at the design stage of RCTs so that results are readily transferable to 'real world' practice
Spatial scales of COVID-19 transmission in Mexico
During outbreaks of emerging infectious diseases, internationally connected cities often experience large and early outbreaks, while rural regions follow after some delay. This hierarchical structure of disease spread is influenced primarily by the multiscale structure of human mobility. However, during the COVID-19 epidemic, public health responses typically did not take into consideration the explicit spatial structure of human mobility when designing nonpharmaceutical interventions (NPIs). NPIs were applied primarily at national or regional scales. Here, we use weekly anonymized and aggregated human mobility data and spatially highly resolved data on COVID-19 cases at the municipality level in Mexico to investigate how behavioral changes in response to the pandemic have altered the spatial scales of transmission and interventions during its first wave (March–June 2020). We find that the epidemic dynamics in Mexico were initially driven by exports of COVID-19 cases from Mexico State and Mexico City, where early outbreaks occurred. The mobility network shifted after the implementation of interventions in late March 2020, and the mobility network communities became more disjointed while epidemics in these communities became increasingly synchronized. Our results provide dynamic insights into how to use network science and epidemiological modeling to inform the spatial scale at which interventions are most impactful in mitigating the spread of COVID-19 and infectious diseases in general
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Paired SARS-CoV-2 spike protein mutations observed during ongoing SARS-CoV-2 viral transfer from humans to minks and back to humans
A mutation analysis of SARS-CoV-2 genomes collected around the world sorted by sequence, date, geographic location, and species has revealed a large number of variants from the initial reference sequence in Wuhan. This analysis also reveals that humans infected with SARS-CoV-2 have infected mink populations in the Netherlands, Denmark, United States, and Canada. In these animals, a small set of mutations in the spike protein receptor binding domain (RBD), often occurring in specific combinations, has transferred back into humans. The viral genomic mutations in minks observed in the Netherlands and Denmark show the potential for new mutations on the SARS-CoV-2 spike protein RBD to be introduced into humans by zoonotic transfer. Our data suggests that close attention to viral transfer from humans to farm animals and pets will be required to prevent build-up of a viral reservoir for potential future zoonotic transfer
Reconfiguration, contestation, and decline: conceptualizing mature large technical systems
Large technical systems (LTS) are integral to modern lifestyles but arduous to analyze. In this paper, we advance a conceptualization of LTS using the notion of mature “phases,” drawing from insights into innovation studies, science and technology studies, political science, the sociology of infra- structure, history of technology, and governance. We begin by defining LTS as a unit of analysis and explaining its conceptual utility and novelty, situating it among other prominent sociotechnical theories. Next, we argue that after LTS have moved through the (overlapping) phases proposed by Thomas Hughes of invention, expansion, growth, momentum, and style,mature LTS undergo the additional (overlapping) phases of reconfiguration, contestation (subject to pressures such as drift and crisis), and eventually stagnation and decline. We illustrate these analytical phases with historical case studies and the conceptual literature, and close by suggesting future research to refine and develop the LTS framework, particularly related to more refined typologies, temporal dimensions, and a broadening of system users. We aim to contribute to theoretical debates about the coevolution of LTS as well as empirical discussions about system-related use, socio- technical change, and policy-making
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