1,236 research outputs found
Optimizing urothelial cell preparation for the human urinary micronucleus assay
Biological monitoring of early genotoxic effects in urothelial cells using the urinary micronucleus (MNu) assay is promising for early detection of cancer, such as bladder carcinoma. But many problems are encountered, the major being the poorly differential staining of cells, particularly in women having an important amount of squamous cells. We have optimized the protocol and obtained a differential staining of the cell types present in urine on 10 subjects. Following Carnoy I fixation and Papanicolaou staining, urothelial cells were blue while most squamous cells were pink. This differential staining allowed for optimization of the MNu assay on a single urine void, for both females and males. Even if our MNu means were comparable to the literature, the great variation in reported MNu results could reside in the ability of scorers to distinguish correctly between urothelial and squamous cells. When monitoring exposed populations, this erroneous distinction could largely influence the results, even more in women’s urine samples. Given a situation where exposure would not increase micronuclei frequency in vaginal squamous cells, their erroneous analysis in the MNu assay could mask an early genotoxic effect. Therefore, as transitional cell carcinoma of the bladder originates from transformed urothelial cells, restricting micronuclei analysis to urothelial cells could yield a more precise estimate of cancer risk in exposed populations. Moreover, it is hoped that the improvements proposed in this paper will allow for an easier implementation of the MNu assay in various set-ups and enhance its specificity, since MNu are considered a suitable biomarker
Chandra Observations of Galaxy Zoo Mergers: Frequency of Binary Active Nuclei in Massive Mergers
We present the results from a Chandra pilot study of 12 massive galaxy
mergers selected from Galaxy Zoo. The sample includes major mergers down to a
host galaxy mass of 10 that already have optical AGN
signatures in at least one of the progenitors. We find that the coincidences of
optically selected active nuclei with mildly obscured ( cm) X-ray nuclei are relatively common (8/12), but the
detections are too faint ( counts per nucleus; erg s cm) to reliably separate starburst and
nuclear activity as the origin of the X-ray emission. Only one merger is found
to have confirmed binary X-ray nuclei, though the X-ray emission from its
southern nucleus could be due solely to star formation. Thus, the occurrences
of binary AGN in these mergers are rare (0-8%), unless most merger-induced
active nuclei are very heavily obscured or Compton thick.Comment: 8 pages, including 5 figures and 1 table. Accepted by Ap
A Hard Look at NGC 5347: Revealing a Nearby Compton-thick AGN
Current measurements show that the observed fraction of Compton-thick (CT) active galactic nuclei (AGN) is smaller than the expected values needed to explain the cosmic X-ray background. Prior fits to the X-ray spectrum of the nearby Seyfert-2 galaxy NGC 5347 (z = 0.00792, D = 35.5 Mpc ) have alternately suggested a CT and Compton-thin source. Combining archival data from Suzaku, Chandra, and—most importantly—new data from NuSTAR, ... See full text for complete abstrac
IC 751: a new changing-look AGN discovered by NuSTAR
We present the results of five NuSTAR observations of the type 2 active
galactic nucleus (AGN) in IC 751, three of which were performed simultaneously
with XMM-Newton or Swift/XRT. We find that the nuclear X-ray source underwent a
clear transition from a Compton-thick () to a Compton-thin () state on timescales of months, which makes
IC 751 the first changing-look AGN discovered by NuSTAR. Changes of the
line-of-sight column density at a level are also found on a
time-scale of hours ().
From the lack of spectral variability on timescales of ks we infer
that the varying absorber is located beyond the emission-weighted average
radius of the broad-line region, and could therefore be related either to the
external part of the broad-line region or a clumpy molecular torus. By adopting
a physical torus X-ray spectral model, we are able to disentangle the column
density of the non-varying absorber () from that of the varying clouds
[], and to constrain that of
the material responsible for the reprocessed X-ray radiation (). We find evidence of significant intrinsic X-ray
variability, with the flux varying by a factor of five on timescales of a few
months in the 2-10 and 10-50 keV band.Comment: Accepted for publication in ApJ, 11 pages, 6 figure
Crossover Scaling in Dendritic Evolution at Low Undercooling
We examine scaling in two-dimensional simulations of dendritic growth at low
undercooling, as well as in three-dimensional pivalic acid dendrites grown on
NASA's USMP-4 Isothermal Dendritic Growth Experiment. We report new results on
self-similar evolution in both the experiments and simulations. We find that
the time dependent scaling of our low undercooling simulations displays a
cross-over scaling from a regime different than that characterizing Laplacian
growth to steady-state growth
Optical, near-IR and sub-mm IFU Observations of the nearby dual AGN Mrk 463
We present optical and near-IR Integral Field Unit (IFU) and ALMA band 6
observations of the nearby dual Active Galactic Nuclei (AGN) Mrk 463. At a
distance of 210 Mpc, and a nuclear separation of 4 kpc, Mrk 463 is an
excellent laboratory to study the gas dynamics, star formation processes and
supermassive black hole (SMBH) accretion in a late-stage gas-rich major galaxy
merger. The IFU observations reveal a complex morphology, including tidal
tails, star-forming clumps, and emission line regions. The optical data, which
map the full extent of the merger, show evidence for a biconical outflow and
material outflowing at 600 km s, both associated with the Mrk 463E
nucleus, together with large scale gradients likely related to the ongoing
galaxy merger. We further find an emission line region 11 kpc south of
Mrk 463E that is consistent with being photoionized by an AGN. Compared to the
current AGN luminosity, the energy budget of the cloud implies a luminosity
drop in Mrk 463E by a factor 3-20 over the last 40,000 years. The ALMA
observations of CO(2-1) and adjacent 1mm continuum reveal the presence
of 10M in molecular gas in the system. The molecular gas
shows velocity gradients of 800 km/s and 400 km/s around the Mrk
463E and 463W nuclei, respectively. We conclude that in this system the infall
of 100s /yr of molecular gas is in rough balance with the
removal of ionized gas by a biconical outflow being fueled by a relatively
small, 0.01% of accretion onto each SMBH.Comment: Accepted by The Astrophysical Journal, 23 pages, 19 figure
X-ray bolometric corrections for Compton-thick active galactic nuclei
We present X-ray bolometric correction factors, (), for Compton-thick (CT) active galactic nuclei (AGN) with the aim
of testing AGN torus models, probing orientation effects, and estimating the
bolometric output of the most obscured AGN. We adopt bolometric luminosities,
, from literature infrared (IR) torus modeling and compile published
intrinsic 2--10 keV X-ray luminosities, , from X-ray torus modeling of
NuSTAR data. Our sample consists of 10 local CT AGN where both of these
estimates are available. We test for systematic differences in
values produced when using two widely used IR torus models and two widely used
X-ray torus models, finding consistency within the uncertainties. We find that
the mean of our sample in the range
erg/s is log
with an intrinsic scatter of dex, and that our derived
values are consistent with previously established relationships between
and and and Eddington ratio. We
investigate if is dependent on by comparing our results on
CT AGN to published results on less-obscured AGN, finding no significant
dependence. Since many of our sample are megamaser AGN, known to be viewed
edge-on, and furthermore under the assumptions of AGN unification whereby
unobscured AGN are viewed face-on, our result implies that the X-ray emitting
corona is not strongly anisotropic. Finally, we present values
for CT AGN identified in X-ray surveys as a function of their observed ,
where an estimate of their intrinsic is not available, and redshift,
useful for estimating the bolometric output of the most obscured AGN across
cosmic time.Comment: Accepted for publication in Ap
- …