АДАПТАЦІЯ МЕТОДИЧНИХ МАТЕРІАЛІВ ДО ПРАКТИЧНОГО ЗАНЯТТЯ НА ТЕМУ “ПРОВЕДЕННЯ УРОКУ ЗДОРОВ’Я” НА ІІІ КУРСІ СТОМАТОЛОГІЧНОГО ФАКУЛЬТЕТУ ДЛЯ ПІДВИЩЕННЯ ЙОГО ПРАКТИЧНОГО СПРЯМУВАННЯ

There are advantages of the credit-module system , but at practical lessons some principles might be skip . According to the  program of “Prophylaxis of stomatological disorders” at the III course of stomatological faculty the subject of  the practical lesson № 17-18 is a conduction of the “Health lesson” that should be managed within four academic hours. This practical lesson should be held in the organized children’s groups. To enhance its practical guide we have introduce some changes to the “Part VI. Plan and management of the lesson” at the Pediatric Stomatology Department of Ivano-Frankivsk National Medical University. The dress rehearsal is operated before the lesson instead of the test control and oral part and the main part is a performance itself. Besides, the initial and the final level of the test control should be administer at the previous lesson. Therefore, such innovation of methodical materials meets all practical requirements and provides the students and pedagogues with massive options of creativity.  // o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "ojs.tdmu.edu.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;e// o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "ojs.tdmu.edu.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;eКредитно-модульна система освіти має свої переваги, але є практичні заняття, на яких необхідно відступити від деяких її принципів. На ІІІ курсі стоматологічного факультету при вивченні предмета “Профілактика стоматологічних захворювань” згідно з робочою програмою є практичне заняття № 17-18 на тему “Проведення уроку здоров’я”, на яке відводиться чотири академічні години. Це заняття передбачає роботу студентів в організованих дитячих колективах. Із метою повного його практичного спрямування на кафедрі дитячої стоматології Івано-Франківського національного медичного університету внесено зміни в “Розділ VI. План і організаційна структура заняття” до цієї методичної розробки, що полягають у проведенні генеральної репетиції уроку здоров’я замість опитування і тестування, а основна частина заняття відводиться на власне виступ – урок здоров’я. При цьому вихідний та кінцевий рівень знань студентів викладач з’ясовує на попередньому занятті. Проведена нами адаптація методичних матеріалів допомагає отримати повне практичне спрямування цього практичного заняття та дозволяє студентам і викладачу цілком віддатися творчій праці.// o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "ojs.tdmu.edu.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;

A nearly complete database on the records and ecology of the rarest boreal tiger moth from 1840s to 2020

Global environmental changes may cause dramatic insect declines but over century-long time series of certain species’ records are rarely available for scientific research. The Menetries’ Tiger Moth (Arctia menetriesii) appears to be the most enigmatic example among boreal insects. Although it occurs throughout the entire Eurasian taiga biome, it is so rare that less than 100 specimens were recorded since its original description in 1846. Here, we present the database, which contains nearly all available information on the species’ records collected from 1840s to 2020. The data on A. menetriesii records (N = 78) through geographic regions, environments, and different timeframes are compiled and unified. The database may serve as the basis for a wide array of future research such as the distribution modeling and predictions of range shifts under climate changes. It represents a unique example of a more than century-long dataset of distributional, ecological, and phenological data designed for an exceptionally rare but widespread boreal insect, which primarily occurs in hard-to-reach, uninhabited areas of Eurasia.Peer reviewe

TRPM2-mediated rise in mitochondrial Zn2+ promotes palmitate-induced mitochondrial fission and pancreatic β-cell death in rodents

Rise in plasma free fatty acids (FFAs) represents a major risk factor for obesity-induced type 2 diabetes. Saturated FFAs cause a progressive decline in insulin secretion by promoting pancreatic β-cell death through increased production of reactive oxygen species (ROS). Recent studies have demonstrated that palmitate (a C16-FFA)-induced rise in ROS causes β-cell death by triggering mitochondrial fragmentation, but the underlying mechanisms are unclear. Using the INS1-832/13 β-cell line, here we demonstrate that palmitate generates the ROS required for mitochondrial fission by activating NOX (NADPH oxidase)-2. More importantly, we show that chemical inhibition, RNAi-mediated silencing and knockout of ROS-sensitive TRPM (transient receptor potential melastatin)-2 channels prevent palmitate-induced mitochondrial fission. Although TRPM2 activation affects the intracellular dynamics of Ca2+ and Zn2+, chelation of Zn2+ alone was sufficient to prevent mitochondrial fission. Consistent with the role of Zn2+, palmitate caused a rise in mitochondrial Zn2+, leading to Zn2+-dependent mitochondrial recruitment of Drp-1 (a protein that catalyses mitochondrial fission) and loss of mitochondrial membrane potential. In agreement with the previous reports, Ca2+ caused Drp-1 recruitment, but it failed to induce mitochondrial fission in the absence of Zn2+. These results indicate a novel role for Zn2+ in mitochondrial dynamics. Inhibition or knockout of TRPM2 channels in mouse islets and RNAi-mediated silencing of TRPM2 expression in human islets prevented FFA/cytokine-induced β-cell death, findings that are consistent with the role of abnormal mitochondrial fission in cell death. To conclude, our results reveal a novel, potentially druggable signalling pathway for FFA-induced β-cell death. The cascade involves NOX-2-dependent production of ROS, activation of TRPM2 channels, rise in mitochondrial Zn2+, Drp-1 recruitment and abnormal mitochondrial fission

Insulin Storage and Glucose Homeostasis in Mice Null for the Granule Zinc Transporter ZnT8 and Studies of the Type 2 Diabetes–Associated Variants

International audienceObjective. Zinc ions are essential for the formation of hexameric insulin and hormone crystallisation. Correspondingly, a non-synonymous single nucleotide polymorphism rs13266634 in the SLC30A8 gene, encoding the secretory granule zinc transporter ZnT8, is associated with type 2 diabetes. Here, we describe the effects of deleting the ZnT8 gene in mice and explore the action of the at-risk allele. Research Design and Methods. Slc30a8 null mice were generated and backcrossed at least twice onto a C57BL/6J background. Glucose and insulin tolerance were measured by intraperitoneal injection, or euglycemic clamp, respectively. Insulin secretion, electrophysiology, imaging, and the generation of adenoviruses encoding the low- (W325) or elevated- (R325) risk ZnT8 alleles, were undertaken using standard protocols. Results. ZnT8(-/-) mice displayed age, sex and diet-dependent abnormalities in glucose tolerance, insulin secretion and body weight. Islets isolated from null mice had reduced granule zinc content, and showed age-dependent changes in granule morphology, with markedly fewer dense cores but more rod-like crystals. Glucose-stimulated insulin secretion, granule fusion and insulin crystal dissolution, as assessed by total internal reflection fluorescence microscopy, were unchanged or enhanced in ZnT8(-/-) islets. Insulin processing was normal. Molecular modelling revealed that residue-325 was located at the interface between ZnT8 monomers. Correspondingly, the R325 variant displayed lower apparent Zn(2+) transport activity than W325 ZnT8 by fluorescence-based assay. Discussion and conclusions. ZnT8 is required for normal insulin crystallisation and insulin release in vivo but not, remarkably, in vitro. Defects in the former processes in carriers of the R allele may increase type 2 diabetes risk

A large topographic feature on the surface of the trans-Neptunian object (307261) 2002 MS4_4 measured from stellar occultations

This work aims at constraining the size, shape, and geometric albedo of the dwarf planet candidate 2002 MS4 through the analysis of nine stellar occultation events. Using multichord detection, we also studied the object's topography by analyzing the obtained limb and the residuals between observed chords and the best-fitted ellipse. We predicted and organized the observational campaigns of nine stellar occultations by 2002 MS4 between 2019 and 2022, resulting in two single-chord events, four double-chord detections, and three events with three to up to sixty-one positive chords. Using 13 selected chords from the 8 August 2020 event, we determined the global elliptical limb of 2002 MS4. The best-fitted ellipse, combined with the object's rotational information from the literature, constrains the object's size, shape, and albedo. Additionally, we developed a new method to characterize topography features on the object's limb. The global limb has a semi-major axis of 412 $\pm$ 10 km, a semi-minor axis of 385 $\pm$ 17 km, and the position angle of the minor axis is 121 $^\circ$ $\pm$ 16$^\circ$. From this instantaneous limb, we obtained 2002 MS4's geometric albedo and the projected area-equivalent diameter. Significant deviations from the fitted ellipse in the northernmost limb are detected from multiple sites highlighting three distinct topographic features: one 11 km depth depression followed by a 25$^{+4}_{-5}$ km height elevation next to a crater-like depression with an extension of 322 $\pm$ 39 km and 45.1 $\pm$ 1.5 km deep. Our results present an object that is $\approx$138 km smaller in diameter than derived from thermal data, possibly indicating the presence of a so-far unknown satellite. However, within the error bars, the geometric albedo in the V-band agrees with the results published in the literature, even with the radiometric-derived albedo

50 years of the first mammary-coronary bypass grafting using microsurgical techniques and operating microscope in human (George E. Green, 1968)

The use of an internal mammary artery in coronary artery bypass grafting operations is the gold standard nowadays. One of the pioneers of such a technique is George E. Green. He introduced a microsurgical technique in routine clinical practice when performing direct myocardial revascularization. The article presents a brief biography and data on the professional path of George E. Green, as well as the history of development and microscope-based performance of the first mammary-coronary bypass grafting operation, which was done in 1968

THE SET OF METHODS AND ALGORITHMS FOR THE REMOTE PHOTOMETRIC AND COORDINATE MONITORING OF COSMIC OBJECTS

The paper is devoted to elaboration of the new optimized methods, algorithms and programs for the solution of problems of the remote control of near-earth space (CS), which are related to investigation of cosmic objects (CO) based on the photometric and coordinate measurements. It is shown, that for these determination of satellite's rotation period around center of mass, the orientation of rotation axis, and also its color, polarization and others characteristics can be used. The main principles of a system of the CO identification based on their light curves are elaborated. A new algorithm of CO orbit determination using the network station data is created. Its accuracy is by 3-4 orders at magnitude higher than that of algorithms based on the well-known methods of celestial mechanics

THE STUDY OF TIMING RELATIONSHIPS WHICH ARISE WHEN USING A TELEVISION CCD CAMERA WATEC WAT-902H2 SUPREME IN ASTRONOMICAL INVESTIGATIONS

The present paper describes the study of timing relationships which arise when using an analogue CCD camera Watec WAT-902H2 Sup in astronomical investigations, particularly in time-domain measurements of LEO satellites which are fast-moving against stellar background