COMPORTAMENTO PRODUTIVO DE DIFERENTES GENÓTIPOS DE SOJA NA REGIÃO OESTE DO PARANÁ

The agribusiness sector is responsible for the largest percentage of the Brazilian GDP; nevertheless, agricultural activities have a low return rate and a high cost risk. To meet the financial needs of producers and agribusiness sectors, the choice of the genotype to be planted in commercial areas is one of the first decisions to be made. This study aimed to evaluate the production components of five different soybean genotypes in the western region of Paraná in the 2022/2023 season under standardized planting conditions. The experimental design used was a randomized complete block (RCB) with 5 treatments and 4 replications, totaling 20 experimental plots. The treatments consisted of planting different soybean genotypes (M 6100 XTD, AS 3615 I2X, P 96R10 IPRO, DM 60IX64 RSF I2X, and M 6110 I2X), all of indeterminate growth and early maturity. The genotypes AS 3615 I2X, DM 60IX64 RSF I2X, and M 6110 I2X have the Intacta2 Xtend technology, while M 6100 XTD has the Xtend Biotec technology, and P 96R10 has the Intacta RR2 PRO technology. Furthermore, all materials belong to the relative maturity group (GMR) 6.1, except for DM 60IX64 RSF I2X, which is in the GMR 6.0 group. The following parameters were evaluated: plant height (AP), number of racemes (NR), number of pods per plant (NVP), number of grains per pod (NGV), thousand-grain weight (MMG), productivity (PROD), and vegetative index (IV). After measuring the mentioned parameters, the results were subjected to the Tukey statistical test to compare the means, where significant differences (p<0.05) were observed among the genotypes (M 6100 XTD, AS 3615 I2X, P 96R10 IPRO, DM 60IX64 RSF I2X, and M 6110 I2X) for the morphological and productive variables NGV, MMG, and IV. For the productive component NGV, the genotype P 96R10 IPRO had the lowest mean (2.32 grains per pod) compared to the others. However, the same genotype had a higher mean MMG (153.59 g) when compared to AS 3615 I2X and DM 60IX64 RSF I2X. The variables that showed significant differences among the genotypes did not affect the final PROD of the experiment's genotypes because the evaluated genotypes have a high production capacity but different technologies. As there was no chemical management disruption, significant pest and disease attacks, or adverse weather conditions, all genotypes were able to develop their maximum production capacity.O agronegócio é responsável pelo maior percentual do PIB brasileiro, ainda assim, as atividades agrícolas têm uma baixa taxa de retorno e um alto risco de custo. Para suprir as necessidades financeiras dos produtores e dos setores do agronegócio, a escolha do genótipo a ser implantado nas áreas comerciais é uma das primeiras decisões a serem tomadas. Este trabalho teve como objetivo avaliar os componentes de produção de cinco diferentes genótipos de soja na região oeste paranaense, na safra de 2022/2023, em condições padronizadas de semeadura. O delineamento experimental utilizado foi o de blocos casualizado (DBC), com 5 tratamentos e 4 repetições, totalizando 20 parcelas experimentais. Os tratamentos consistiram na semeadura de diferentes genótipos de soja (M 6100 XTD, AS 3615 I2X, P 96R10 IPRO, DM 60IX64 RSF I2X e M 6110 I2X), todos de crescimento indeterminado e ciclo precoce. Os genótipos AS 3615 I2X, DM 60IX64 RSF I2X e M 6110 I2X possuem a tecnologia Intacta2 Xtend, enquanto o M 6100 XTD possui a tecnologia Xtend Biotec e o P 96R10 a tecnologia Intacta RR2 PRO. Além disso, todos os materiais são do grupo de maturação relativa (GMR) 6.1, com exceção do DM 60IX64 RSF I2X, que é do GMR 6.0. Foram avaliados os parâmetros altura de planta (AP), número de rácemos (NR), número de vagens por planta (NVP), número de grãos por vagem (NGV), massa de mil grãos (MMG), produtividade (PROD) e índice vegetativo (IV). Após a mensuração dos parâmetros citados, os resultados foram submetidos ao teste estatístico de tukey, para comparação das médias, onde foi observado diferenças significativas (p<0,05) entre os genótipos (M 6100 XTD, AS 3615 I2X, P 96R10 IPRO, DM 60IX64 RSF I2X e M 6110 I2X) para as variáveis morfológicas e produtivas NGV, MMG e IV.  Para o componente produtivo NGV, o genótipo P 96R10 IPRO apresentou menor média (2,32 grãos por vagem) em relação aos demais. No entanto, o mesmo genótipo apresentou maior média de MMG (153,59 g), quando comparado ao AS 3615 I2X e ao DM 60IX64 RSF I2X. As variáveis que apresentaram diferença significativa entre os genótipos não interferiram na PROD final dos genótipos do experimento, pois os genótipos avaliados apresentam alta capacidade produtiva, mas tecnologias diferentes. Como não houve descontrole no manejo químico, nem ataques expressivos de pragas e doenças, ou ainda intempéries climáticas, todos os genótipos puderam desenvolver sua máxima capacidade produtiva

Leading Infrared Logarithms from Unitarity, Analyticity and Crossing

We derive non-linear recursion equations for the leading infrared logarithms in massless non-renormalizable effective field theories. The derivation is based solely on the requirements of the unitarity, analyticity and crossing symmetry of the amplitudes. That emphasizes the general nature of the corresponding equations. The derived equations allow one to compute leading infrared logarithms to essentially unlimited loop order without performing a loop calculation. For the implementation of the recursion equation one needs to calculate tree diagrams only. The application of the equation is demonstrated on several examples of effective field theories in four and higher space-time dimensions.Comment: 12 page

A short treatment of cells with the lanthanide ions La3+, Ce3+, Pr3+ or Nd3+ changes the cellular chemistry into a state in which RNA replication of flaviviruses is specifically blocked without interference with host-cell multiplication

Alpha- and flaviviruses contain class II fusion proteins, which form ion-permeable pores in the target membrane during virus entry. The pores generated during entry of the alphavirus Semliki Forest virus have been shown previously to be blocked by lanthanide ions. Here, analyses of the influence of rare earth ions on the entry of the flaviviruses West Nile virus and Uganda S virus revealed an unexpected effect of lanthanide ions. The results showed that a 30 s treatment of cells with an appropriate lanthanide ion changed the cellular chemistry into a state in which the cells no longer supported the multiplication of flaviviruses. This change occurred in cells treated before, during or after infection, did not inhibit multiplication of Semliki Forest virus and did not interfere with host-cell multiplication. The change was generated in vertebrate and insect cells, and was elicited in the presence of actinomycin D. In vertebrate cells, the change was elicited specifically by La3+, Ce3+, Pr3+ and Nd3+. In insect cells, additional lanthanide ions had this activity. Further analyses showed that lanthanide ion treatment blocked the ability of the host cell to support the replication of flavivirus RNA. These results open two areas of research: the study of molecular alterations induced by lanthanide ion treatment in uninfected cells and the analysis of the resulting modifications of the flavivirus RNA replicase complex. The findings possibly open the way for the development of a general chemotherapy against flavivirus diseases such as Dengue fever, Japanese encephalitis, West Nile fever and yellow fever

High Resolution Electron Beam Induced Current Measurements in an Scanning Tunneling Microscope on GaAs-MESFET

Recently, the first results of electron beam induced current (EBIC) measurements in a scanning tunneling microscope (STM) have been reported. Although the acquired results match with those obtained in conventional EBIC investigations, the interpretation of the obtained results is still restricted solely to a qualitative discussion. In this paper, a quantitative approach is used for two-dimensional numerical simulations of induced currents in GaAs-MESFET leading to a first starting point for a sophisticated interpretation of the dependence of induced currents on experimental and device parameters

Development of an Scanning Tunneling Microscopy-Based Electron Beam Induced Current (EBIC) Microscope

Measurements of electron beam induced currents (EBIC) can either be performed in a scanning electron microscope (SEM) or in a scanning tunneling microscope (STM), since both microscopes are very similar in their basic assembly. However, a straightforward application of an STM in EBIC-measurements, i.e. the use of a microscope tip as a fine source for low energetic electrons is not possible due to the specific demands on the instrument in an EBIC application. The present paper gives a compilation of these demands and describes their conversion into an optimized STM-EBIC microscope

Phosphorylation of ezrin on Thr567 is required for the synergistic activation of cell spreading by EPAC1 and protein kinase A in HEK293T cells

Recent studies have demonstrated that the actin binding protein, ezrin, and the cAMP-sensor, EPAC1, cooperate to induce cell spreading in response to elevations in intracellular cAMP. To investigate the mechanisms underlying these effects we generated a model of EPAC1-dependent cell spreading based on the stable transfection of EPAC1 into HEK293T (HEK293T–EPAC1) cells. We found that direct activation of EPAC1 with the EPAC-selective analogue, 8-pCPT-2′-O-Me-cAMP (007), promoted cell spreading in these cells. In addition, co-activation of EPAC1 and PKA, with a combination of the adenylate cyclase activator, forskolin, and the cAMP phosphodiesterase inhibitor, rolipram, was found to synergistically enhance cell spreading, in association with cortical actin bundling and mobilisation of ezrin to the plasma membrane. PKA activation was also associated with phosphorylation of ezrin on Thr567, as detected by an electrophoretic band mobility shift during SDS-PAGE. Inhibition of PKA activity blocked ezrin phosphorylation and reduced the cell spreading response to cAMP elevation to levels induced by EPAC1-activation alone. Transfection of HEK293T–EPAC1 cells with inhibitory ezrin mutants lacking the key PKA phosphorylation site, ezrin-Thr567Ala, or the ability to associate with actin, ezrin-Arg579Ala, promoted cell arborisation and blocked the ability of EPAC1 and PKA to further promote cell spreading. The PKA phospho-mimetic mutants of ezrin, ezrin-Thr567Asp had no effect on EPAC1-driven cell spreading. Our results indicate that association of ezrin with the actin cytoskeleton and phosphorylation on Thr567 are required, but not sufficient, for PKA and EPAC1 to synergistically promote cell spreading following elevations in intracellular cAMP

Thomas A. Williams, Mallarmé and the language of mysticism, Athens, University of Georgia Press, 1970, 99 p.

<div><p>Norepinephrine, a neuromodulator that activates β-adrenergic receptors (βARs), facilitates learning and memory as well as the induction of synaptic plasticity in the hippocampus. Several forms of long-term potentiation (LTP) at the Schaffer collateral CA1 synapse require stimulation of both βARs and <i>N</i>-methyl-<i>D</i>-aspartate receptors (NMDARs). To understand the mechanisms mediating the interactions between βAR and NMDAR signaling pathways, we combined FRET imaging of cAMP in hippocampal neuron cultures with spatial mechanistic modeling of signaling pathways in the CA1 pyramidal neuron. Previous work implied that cAMP is synergistically produced in the presence of the βAR agonist isoproterenol and intracellular calcium. In contrast, we show that when application of isoproterenol precedes application of NMDA by several minutes, as is typical of βAR-facilitated LTP experiments, the average amplitude of the cAMP response to NMDA is attenuated compared with the response to NMDA alone. Models simulations suggest that, although the negative feedback loop formed by cAMP, cAMP-dependent protein kinase (PKA), and type 4 phosphodiesterase may be involved in attenuating the cAMP response to NMDA, it is insufficient to explain the range of experimental observations. Instead, attenuation of the cAMP response requires mechanisms upstream of adenylyl cyclase. Our model demonstrates that Gs-to-Gi switching due to PKA phosphorylation of βARs as well as Gi inhibition of type 1 adenylyl cyclase may underlie the experimental observations. This suggests that signaling by β-adrenergic receptors depends on temporal pattern of stimulation, and that switching may represent a novel mechanism for recruiting kinases involved in synaptic plasticity and memory.</p></div

Leading infrared logarithms for sigma-model with fields on arbitrary Riemann manifold

We derive non-linear recursion equation for the leading infrared logarithms (LL) in four dimensional sigma-model with fields on an arbitrary Riemann manifold. The derived equation allows one to compute leading infrared logarithms to essentially unlimited loop order in terms of geometric characteristics of the Riemann manifold. We reduce the solution of the SU(oo) principal chiral field in arbitrary number of dimensions in the LL approximation to the solution of very simple recursive equation. This result paves a way to the solution of the model in arbitrary number of dimensions at N-->ooComment: Talk given by MVP at the conference devoted to memory of A.N. Vasilie