The distribution and ownership of English chapbooks and other cheap print in south Wales and its borders 1660 to 1730: developmental influences on commerce, religion and education

This thesis examines the distribution and ownership of English-language books in south Wales and the borders between 1660 and 1730, and will test the extent to which there was a direct correlation between the levels of literacy, the development of education, patterns of belief, and commerce. The focus within the book genre is on chapbooks and other types of cheap print. The historic counties under consideration are Brecon, Carmarthen, Glamorgan, Monmouth, Pembroke and the border areas of Hereford and Gloucester. Until 1695, printing remained largely confined to London, so all books reaching south Wales had to travel that distance. An examination of how this distribution was facilitated, and what was the demand for different types of material, is instructive in building a picture of its readership and ownership. Towards the end of the century, the political climate changed in two ways critical to this scenario. First, the printing Act of 1662, which prevented provincial printing, expired in 1695, and secondly the government decided to raise revenue through an Act of 1697 which required hawkers and pedlars to purchase an annual licence. So, from the closing years of seventeenth century, there was a slow burgeoning of provincial printing. In western regions of Britain, there were presses in Bristol (1695), Cirencester (1720), Gloucester (1722) and Carmarthenshire (1721) and their output included small books and newspapers. This study will consider the appetite of the people of south Wales and borders for popular cheap printed material in the English language, and produce evidence as to its effect on religious and educational development

How effective is ‘greening’ of urban areas in reducing human exposure to ground-level ozone concentrations, UV exposure and the ‘urban heat island effect’? An updated systematic review

Abstract Background This review updates a systematic review published in 2010 ( http://www.environmentalevidence.org/completed-reviews/how-effective-is-greening-of-urban-areas-in-reducing-human-exposure-to-ground-level-ozone-concentrations-uv-exposure-and-the-urban-heat-island-effect ) which addressed the question: How effective is ‘greening’ of urban areas in reducing human exposure to ground-level ozone concentrations, UV exposure and the ‘urban heat island effect’? Methods Searches of multiple databases and journals for relevant published articles and grey literature were conducted. Organisational websites were searched for unpublished articles. Eligibility criteria were applied at title, abstract and full text and included studies were critically appraised. Consistency checks of these processes were undertaken. Pre-defined data items were extracted from included studies. Quantitative synthesis was performed through meta-analysis and narrative synthesis was undertaken. Review findings 308 studies were included in this review. Studies were spread across all continents and climate zones except polar but were mainly concentrated in Europe and temperate regions. Most studies reported on the impact of urban greening on temperature with fewer studies reporting data on ground-level UV radiation, ozone concentrations (or precursors) or public health indicators. The findings of the original review were confirmed; urban green areas tended to be cooler than urban non-green areas. Air temperature under trees was on average 0.8 °C cooler but treed areas could be warmer at night. Cooling effect showed tree species variation. Tree canopy shading was a significant effect modifier associated with attenuation of solar radiation during the day. Urban forests were on average 1.6 °C cooler than comparator areas. Treed areas and parks and gardens were associated with improved human thermal comfort. Park or garden cooling effect was on average 0.8 °C and trees were a significant influence on this during the day. Park or garden cooling effect extended up to 1.25 kms beyond their boundaries. Grassy areas were cooler than non-green comparators, both during daytime and at night, by on average 0.6 °C. Green roofs and walls showed surface temperature cooling effect (2 and 1.8 °C on average respectively) which was influenced by substrate water content, plant density and cover. Ground-level concentrations of nitrogen oxides were on average lower by 1.0 standard deviation units in green areas, with tree species variation in removal of these pollutants and emission of biogenic volatile organic compounds (precursors of ozone). No clear impact of green areas on ground level ozone concentrations was identified. Conclusions Design of urban green areas may need to strike a balance between maximising tree canopy shading for day-time thermal comfort and enabling night-time cooling from open grassy areas. Choice of tree species needs to be guided by evapotranspiration potential, removal of nitrogen oxides and emission of biogenic volatile organic compounds. Choice of plant species and substrate composition for green roofs and walls needs to be tailored to local thermal comfort needs for optimal effect. Future research should, using robust study design, address identified evidence gaps and evaluate optimal design of urban green areas for specific circumstances, such as mitigating day or night-time urban heat island effect, availability of sustainable irrigation or optimal density and distribution of green areas. Future evidence synthesis should focus on optimal design of urban green areas for public health benefit

Phosphorus and nitrogen limitation and impairment of headwater streams relative to rivers in Great Britain: a national perspective on eutrophication

This study provides a first national-scale assessment of the nutrient status of British headwater streams within the wider river network, by joint analysis of the national Countryside Survey Headwater Stream and Harmonised River Monitoring Scheme datasets. We apply a novel Nutrient Limitation Assessment methodology to explore the extent to which nutrients may potentially limit primary production in headwater streams and rivers, by coupling ternary assessment of nitrogen (N), phosphorus (P), and carbon (C) depletion, with N:P stoichiometry, and threshold P and N concentrations. P limitation was more commonly seen in the rivers, with greater prevalence of N limitation in the headwater streams. High levels of potential P and N co-limitation were found in the headwater streams, especially the Upland-Low-Alkalinity streams. This suggests that managing both P and N inputs may be needed to minimise risks of degradation of these sensitive headwater stream environments. Although localised nutrient impairment of headwater streams can occur, there were markedly lower rates of P and N impairment of headwater streams relative to downstream rivers at the national scale. Nutrient source contributions, relative to hydrological dilution, increased with catchment scale, corresponding with increases in the extent of agricultural and urban land-use. The estimated nutrient reductions needed to achieve compliance with Water Framework Directive standards, and to reach limiting concentrations, were greatest for the Lowland-High-Alkalinity rivers and streams. Preliminary assessments suggest that reducing P concentrations in the Lowland-High-Alkalinity headwater streams, and N concentrations in the Upland-Low-Alkalinity rivers, might offer greater overall benefits for water-quality remediation at the national scale, relative to the magnitude of nutrient reductions required. This approach could help inform the prioritisation of nutrient remediation, as part of a directional approach to water quality management based on closing the gaps between current and target nutrient concentrations

GAA repeat expansion mutation mouse models of Friedreich ataxia exhibit oxidative stress leading to progressive neuronal and cardiac pathology

Friedreich ataxia (FRDA) is a neurodegenerative disorder caused by an unstable GAA repeat expansion mutation within intron 1 of the FXN gene. However, the origins of the GAA repeat expansion, its unstable dynamics within different cells and tissues, and its effects on frataxin expression are not yet completely understood. Therefore, we have chosen to generate representative FRDA mouse models by using the human FXN GAA repeat expansion itself as the genetically modified mutation. We have previously reported the establishment of two lines of human FXN YAC transgenic mice that contain unstable GAA repeat expansions within the appropriate genomic context. We now describe the generation of FRDA mouse models by crossbreeding of both lines of human FXN YAC transgenic mice with heterozygous Fxn knockout mice. The resultant FRDA mice that express only human-derived frataxin show comparatively reduced levels of frataxin mRNA and protein expression, decreased aconitase activity, and oxidative stress, leading to progressive neurodegenerative and cardiac pathological phenotypes. Coordination deficits are present, as measured by accelerating rotarod analysis, together with a progressive decrease in locomotor activity and increase in weight. Large vacuoles are detected within neurons of the dorsal root ganglia (DRG), predominantly within the lumbar regions in 6-month-old mice, but spreading to the cervical regions after 1 year of age. Secondary demyelination of large axons is also detected within the lumbar roots of older mice. Lipofuscin deposition is increased in both DRG neurons and cardiomyocytes, and iron deposition is detected in cardiomyocytes after 1 year of age. These mice represent the first GAA repeat expansion-based FRDA mouse models that exhibit progressive FRDA-like pathology and thus will be of use in testing potential therapeutic strategies, particularly GAA repeat-based strategies. © 2006 Elsevier Inc. All rights reserved

International meta-analysis of PTSD genome-wide association studies identifies sex- and ancestry-specific genetic risk loci.

The risk of posttraumatic stress disorder (PTSD) following trauma is heritable, but robust common variants have yet to be identified. In a multi-ethnic cohort including over 30,000 PTSD cases and 170,000 controls we conduct a genome-wide association study of PTSD. We demonstrate SNP-based heritability estimates of 5-20%, varying by sex. Three genome-wide significant loci are identified, 2 in European and 1 in African-ancestry analyses. Analyses stratified by sex implicate 3 additional loci in men. Along with other novel genes and non-coding RNAs, a Parkinson's disease gene involved in dopamine regulation, PARK2, is associated with PTSD. Finally, we demonstrate that polygenic risk for PTSD is significantly predictive of re-experiencing symptoms in the Million Veteran Program dataset, although specific loci did not replicate. These results demonstrate the role of genetic variation in the biology of risk for PTSD and highlight the necessity of conducting sex-stratified analyses and expanding GWAS beyond European ancestry populations

Not all waits are equal: An investigation of emergency care patient pathway.

Abstract Background: Increasing pressure in the United Kingdom (UK) urgent care system has led to Emergency Departments (EDs) failing to meet the national requirement that 95% of patients are admitted, discharged or transferred within 4-h of arrival. Despite the target being the same for all acute hospitals, individual Trusts organise their services in different ways. The impact of this variation on patient journey time and waiting is unknown. Our study aimed to apply the Lean technique of Value Stream Mapping (VSM) to investigate care processes and delays in patient journeys at four contrasting hospitals. Methods: VSM timing data were collected for patients accessing acute care at four hospitals in South West England. Data were categorised according to waits and activities, which were compared across sites to identify variations in practice from the patient viewpoint. We included Public and Patient Involvement (PPI) to fully interpret our findings; observations and initial findings were considered in a PPI workshop. Results: One hundred eight patients were recruited, comprising 25,432 min of patient time containing 4098 episodes of care or waiting. The median patient journey was 223 min (3 h, 43 min); just within the 4-h target. Although total patient journey times were similar between sites, the stage where the greatest proportion of waiting occurred varied. Reasons for waiting were dominated by waits for beds, investigations or results to be available. From our sample we observed that EDs without a discharge/clinical decision area exhibited a greater proportion of waiting time following an admission or discharge decision. PPI interpretation indicated that patients who experience waits at the beginning of their journey feel more anxious because they are ‘not in the system yet’. Conclusions: The novel application of VSM analysis across different hospitals, coupled with PPI interpretation, provides important insight into the impact of care provision on patient experience. Measures that could reduce patient waiting include automatic notification of test results, and the option of discharge/clinical decision areas for patients awaiting results or departure. To enhance patient experience, good communication with patients and relatives about reasons for waits is essential. Keywords: Health service research, Acute care, Emergency admissions, Patient care, Value stream mapping, Emergency department, Patient public involvemen

A socio-technical approach to improving retail energy efficiency behaviours

In recent years, the UK retail sector has made a significant contribution to societal responses on carbon reduction. We provide a novel and timely examination of environmental sustainability from a systems perspective, exploring how energy-related technologies and strategies are incorporated into organisational life. We use a longitudinal case study approach, looking at behavioural energy efficiency from within one of the UK's leading retailers. Our data covers a two-year period, with qualitative data from a total of 131 participants gathered using phased interviews and focus groups. We introduce an adapted socio-technical framework approach in order to describe an existing organisational behavioural strategy to support retail energy efficiency. Our findings point to crucial socio-technical and goal-setting factors which both impede and/or enable energy efficient behaviours, these include: tensions linked to store level perception of energy management goals; an emphasis on the importance of technology for underpinning change processes; and, the need for feedback and incentives to support the completion of energy-related tasks. We also describe the evolution of a practical operational intervention designed to address issues raised in our findings. Our study provides fresh insights into how sustainable workplace behaviours can be achieved and sustained over time. Secondly, we discuss in detail a set of issues arising from goal conflict in the workplace; these include the development of a practical energy management strategy to facilitate secondary organisational goals through job redesign