Get involved : stories of the Caribbean postcolonial black middle class and the development of civil society

Indiana University-Purdue University Indianapolis (IUPUI)The main research question of this project is: How do the narratives of Caribbean black middle class civil society within the bounds of the “post-postcolonial” state, explain the evolving yet current environment of local and postcolonial civil society development? Using the Bahamas as a case, this project explores the historical, political, cultural, and social conditions that supported the development of civil society within the context of a postcolonial society. Furthermore, an investigation via in-depth interviews, participation observation, archival, and contemporary document analysis contextualizes the present-day work of civil society leaders in the Bahamas. Methodologically, the project employs narrative analysis to uncover the perspectives, voices, and practices of black middle-class Bahamian civil society offering an unfolding, dynamic, and nuanced approach for understanding the historical legacies and contemporary structure of local civil society and philanthropy. The study focuses on three primary forms of narratives. These include the narratives of the past (historical), the narratives of expressive and aesthetic cultural practices, and the narratives of lived experience. The project locates that the development of civil society is linked to historical and cultural forces. The findings show that that the narratives of history, social, and artistic development foregrounds a hybrid model of civil society development drawn from the experience of slavery, colonialism, decolonization, as well as the emerging structures related to economic and political globalization. Furthermore, observed through resilience narratives, local civil society leaders negotiate the boundaries of hybridity in their understanding of their personal, social, and professional identities as well as the way in which they engage government, the public, as well as local and international funders

Women Give 2019

In Women Give 2019: Gender and Giving Across Communities of Color, the Women’s Philanthropy Institute seeks to understand how generosity links women across racial lines. While some research has examined race and charitable giving, this study is the frst to explore the intersection of race, gender, and giving.1 The study builds on a growing body of research that examines how women and men give. It is now well understood that gender diferences exist in women’s and men’s motivations for and patterns of giving. By focusing on the relationship among race, gender, and philanthropy, this report recognizes the philanthropic eforts of all women and in particular, women in communities of color

Advances in structure elucidation of small molecules using mass spectrometry

The structural elucidation of small molecules using mass spectrometry plays an important role in modern life sciences and bioanalytical approaches. This review covers different soft and hard ionization techniques and figures of merit for modern mass spectrometers, such as mass resolving power, mass accuracy, isotopic abundance accuracy, accurate mass multiple-stage MS(n) capability, as well as hybrid mass spectrometric and orthogonal chromatographic approaches. The latter part discusses mass spectral data handling strategies, which includes background and noise subtraction, adduct formation and detection, charge state determination, accurate mass measurements, elemental composition determinations, and complex data-dependent setups with ion maps and ion trees. The importance of mass spectral library search algorithms for tandem mass spectra and multiple-stage MS(n) mass spectra as well as mass spectral tree libraries that combine multiple-stage mass spectra are outlined. The successive chapter discusses mass spectral fragmentation pathways, biotransformation reactions and drug metabolism studies, the mass spectral simulation and generation of in silico mass spectra, expert systems for mass spectral interpretation, and the use of computational chemistry to explain gas-phase phenomena. A single chapter discusses data handling for hyphenated approaches including mass spectral deconvolution for clean mass spectra, cheminformatics approaches and structure retention relationships, and retention index predictions for gas and liquid chromatography. The last section reviews the current state of electronic data sharing of mass spectra and discusses the importance of software development for the advancement of structure elucidation of small molecules

Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S]

Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse brain. We established a practical experimental procedure for the IMS of GPLs. We demonstrated that optimization of the composition of the matrix solution and spectrum normalization to the total ion current (TIC) is critical. Using our procedure, we simultaneously differentiated and visualized the localizations of specific molecular species of GPLs in mouse brain sections. The results showed that PUFA-containing phosphatidylcholines (PCs) were distributed in a cell-selective manner: arachidonic acid- and docosahexaenoic acid-containing PCs were seen in the hippocampal neurons and cerebellar Purkinje cells, respectively. Furthermore, these characteristic localizations of PUFA-PCs were formed during neuronal maturation. The phenomenon of brain cell-selective production of specific PUFA-GPLs will help elucidate the potential physiological functions of PUFAs in specific brain regions