Optimization of a pulsed-field gel electrophoresis for molecular typing of Proteus mirabilis

Objective: For the detection of outbreaks caused byProteus mirabilis, strains clonal relations are determinedmethods as “pulsed-field gel electrophoresis (PFGE)”.The aim of this study was optimization of a pulsed-fieldgel electrophoresis for molecular typing of P. mirabilis.Methods: In this study, PFGE’ protocol is optimized foruse in molecular typing of P. mirabilis. Phylogenetic analyzesof strains were evaluated with Bionumerics softwaresystem (version 6.01; Applied Maths, Sint-Martens-Latem, Belgium).Results: This protocol compared with Gram-negativebacteria PFGE protocols, NotI enzyme is suitable for thisbacterium. Electrophoresis conditions should be revealedas; - block 1: initial pulse duration 1 sec, ending pulseduration 30 sec, striking angle 120°, the current 6 V/cm2,temperature 14°C, time 8 hours; - block 2: initial pulseduration 30 sec, ending pulse duration 70 sec, strikingangle 120°, the current 6 V/cm2, temperature 14°C, time16 hours; - TBE, pH=8.4.Conclusion: P. mirabilis strains were typed by PFGE andBionumerics analysis program were determined clonal relationships.The procedure was simple, reproducible andsuitable for these bacteria. Also it was evaluated, becauseof reducing time, the solution volumes and enzymes canbe economically. Outbreaks of nosocomial infections dueto bacteria studied assessment and the potential to provideuseful information about the degree of prevalence.This optimized protocol is allowed different centers’ PFGEresults to compare with other laboratories results. J ClinExp Invest 2013; 4 (3): 306-312Key words: Proteus mirabilis, molecular typing, pulsedfieldgel electrophoresis

Examining the relationship between job satisfaction and organizational commitment: A research on public sector healthcare professionals during the pandemic period: İş tatmini ile örgütsel bağlılık arasındaki ilişkinin incelenmesi: Pandemi döneminde kamu sektörü sağlık çalışanları üzerine bir araştırma

The aim of this study is to determine the relationship between the job satisfaction levels of public sector health workers and their organizational commitment. The research was conducted with the participation of health sector employees. Job Satisfaction Scale and Organizational Commitment Scale were used. In the study, job satisfaction and organizational commitment levels were also investigated in terms of demographic variables. The obtained data were analyzed by statistical methods.  Descriptive Statistics, ANOVA, t test, correlation and regression analysis were used. According to the findings; there is a negative relationship between emotional continuance and external satisfaction. There is positive correlation between continuance commitment internal satisfactions, and negatively significant relationship between continuance commitment and external satisfaction. In addition, a significant negative correlation was founded between normative commitment and internal satisfaction, positive significant relationship between normative commitment and external satisfaction. It was determined that continuance commitment and normative commitment variables had a significant effect on the internal job satisfaction of helthcare personnel, while emotional commitment, continuance commitment and normative commitment variables had a significant effect on external job satisfaction. It was found that demographic factors are also effective on job satisfaction and organizational commitment. ​Extended English summary is in the end of Full Text PDF (TURKISH) file.   Özet Bu çalışma ile kamu sektörü sağlık çalışanlarının iş tatmin düzeyleri ile örgütsel bağlılık düzeyleri arasındaki ilişkinin belirlenmesi amaçlanmıştır. Araştırma sağlık sektörü çalışanlarının katılımı ile gerçekleştirilmiştir. İş tatmini ölçeği ve örgütsel bağlılık ölçeği kullanılmıştır. Çalışmada ayrıca iş tatmini ve örgütsel bağlılık düzeyleri demografik değişkenler açısından da araştırılmıştır. Elde edilen veriler istatistiki yöntemler ile analize edilmiştir. Tanımlayıcı İstatistikler, ANOVA, t testi, korelasyon ve regresyon analizi kullanılmıştır.   Araştırma sonucunda elde edilen bulgulara göre; duygusal bağlılık ile dışsal tatmin arasında negatif yönlü bir ilişki vardır. Devam bağlılığı ile içsel tatmin arasında pozitif yönlü, devam bağlılığı ile dışsal tatmin arasında negatif yönlü anlamlı bir ilişki bulunmaktadır. Ayrıca normatif bağlılık ile içsel tatmin arasında negatif yönlü anlamlı bir ilişki ve normatif bağlılık ile dışsal tatmin arasında pozitif yönlü anlamlı bir ilişki bulgulanmıştır. Sağlık personellerinin içsel iş tatminlerine devam bağlılığı ve normative bağlılık değişkenlerinin anlamlı birer etkisi olduğu, dışsal iş tatminlerine duygusal bağlılık, devam bağlılığı ve normatif bağlılık değişkenlerinin anlamlı birer etkisi olduğu tespit edilmiştir. Demografik faktörlerin de iş tatmini ve örgütsel bağlılık üzerinde etkili olduğu bulgulanmıştır

Detekcija Coxiella burnetii u skupnim uzorcima kravljeg, kozjeg i ovčjeg mlijeka pomoću lančane reakcije polimeraze (PCR)

Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows’, goats’, and ewes’ bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of 150 BM samples (50 samples of each cows’, goats’, and ewes’ milk) collected from 15 randomly selected dairy farms in Hatay province were analyzed. The BM samples were taken between January 2012 and July 2013. Bacterial DNA was extracted directly from milk samples. Nine of the BM samples (6 %) were PCR positive; five from cows’ BM, two from ewes’ BM, and two from goats’ BM. Bacterial DNA was detected in 3 of 15 (20 %) dairy farms. Positive results were confirmed by DNA sequencing. The sequencing results of Coxiella DNA extracted from cows’, goats’, and ewes’ milk samples were consistent with the reference strain with 100 %, 99.7 %, 99.8 % homology, respectively. BM samples were found to be contaminated with C. burnetii; therefore, taking hygienic measures is necessary for food safety and public health.Q-groznica je široko rasprostranjena zoonoza koju uzrokuje obligatno unutarstanična bakterija Coxiella burnetii. Ovom studijom planirano je utvrditi prevalenciju C. burnetii u skupnim uzorcima kravljeg, kozjeg i ovčjeg mlijeka pomoću PCR i potvrditi pozitivne rezultate sekvenciranjem DNA. Analizirano je ukupno 150 uzoraka (50 uzoraka kravljeg, kozjeg i ovčjeg), sa 15 nasumično odabranih gospodarstava u pokrajini Hatay. Skupni uzorci uzeti su u razdoblju između siječnja 2012. i srpnja 2013. Bakterijska DNA izolirana je direktno iz mlijeka. Devet je uzoraka (6 %) bilo PCR pozitivno (pet uzoraka kravljeg mlijeka i po dva kozjeg i ovčjeg). Bakterijska DNA utvrđena je u 3 od 15 (20 %) mliječnih far¬mi. Pozitivni rezultati potvrđeni su sekvenciranjem bakterijske DNA, jer je utvrđena vrlo visoka (100 %, 99,7 %, 99,8 %) homolognost sekvenci sa referentnim sojem Coxiella burnetii, stoga je nužno provođenje higijenskih mjera da bi se osigurala sigurnost hrane i zdravlje ljudi

Extended-spectrum ?-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)- producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic ESBL-producing E. coli isolates were identified, eight of them had only the blaTEM-1. Twenty-five ESBL-producing isolates were detected. This research is the first on the investigation and detection of ESBL-producing E. coli isolates from broilers in Turkey. In this study, 8.3% ESBL-producing E. coli were isolated from the cloacal samples of broilers collected from slaughterhouses in Turkey. CTX-M-15 (80%) was the most frequently isolated ESBL type. Using PFGE analysis, it was determined that these isolates had clonal similarity

A Hybrid Approach for Credibility Detection in Twitter

Nowadays, microblogging services are seen as a source of information. It brings us a question. Can we trust information in a microblogging service? In this paper, we focus on one of the popular microblogging services, Twitter, and try to answer which information in Twitter is credible. Newsworthiness, importance and correctness are the dimensions to be measured in this study. We propose a hybrid credibility analysis which combines feature based and graph based approaches. Our model is based on three types of structures, which are tweet, user and topic. Initially, we use feature based learning to construct a prediction model. In the second step, we use the results of this model as input to authority transfer and further refine the credibility scores for each type of node. The same process is used for measuring each of the dimensions of newsworthiness, importance and correctness. Experimental results show that the proposed hybrid method improves the prediction accuracy for each of these credibility dimensions

Determination Of Genotypic Profiles Of Eschericia Colı Strains By Pfge Molecular Method

From the family Enterobacteriaceae is Escherichia coli ( E. Coli) is a pathogenic bacterium that is becoming increasingly important, especially in hospitals, common social areas. Thanks to the broad-spectrum beta-lactams (ESBL) they produce, they are usually multi-resistant and are common in intestinal and urinary tract infections. It is of great importance to reduce cross-contamination from public areas and to examine and reveal the genotype structure of the bacteria in question, i.e. to reach fingerprints, subtype sequences of strains. 50 E. Coli strains selected in the study were typified by Pulse field gel electrophoresis

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Background/aim: The purpose of the study was to evaluate the performance of the VITEK mass spectrometry (MS) (bioMérieux, France) system for the identification of Klebsiella spp. isolated from different sources. Moreover, while assessing the ability of the VITEK 2 automated expert system (AES) to recognize antimicrobial resistance patterns, the researchers have extended the study to compare VITEK 2 with the routine antimicrobial susceptibility testing method. Materials and methods: This study tested 51 Klebsiella spp. isolates that were isolated from environmental examples and clinical examples. Results of conventional methods and the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS were compared. Then, any differing results were compared against a reference 16S rRNA gene sequence, and when indicated, a recA sequencing analysis was done. Results: VITEK MS correctly identified 100% of the Klebsiella spp. isolates. There were two K. oxy toca isolates incorrectly identified to the species level with conventional methods according to the 16S rRNA gene sequencing analysis. In addition, a VITEK 2 AST-N261 card was used for the detection of extended spectrum beta-lactamases (ESBL). Using the VITEK 2 AES, ESBL positivity was found at the rate of 16.3% whereas this rate was 4.08% using the disk diffusion method. Conclusion: MALDI-TOF MS is a rapid and accurate method for the identification of Klebsiella spp. Moreover, the bioMérieux AES provides a useful laboratory tool for the interpretation of susceptibility results

Sıcak Şerit Haddelemede İş Merdane Malzemeleri ve Hadde Merdanesi Döküm Yöntemleri

En etkili üretim proseslerinden biri olan haddelemeyi gerçekleştirmek için kullanılacak malzemelerin seçimi ve bu malzemelerin merdanenin özelliklerine etkisi önemli bir parametredir. Aynı zamanda uygun malzeme ile çeşitli üretim metotlarının kullanılması da bu özellikler üzerinde önemli bir etkiye sahiptir. Hangi uygulamalarda ne çeşit bileşimde merdane malzemesine ihtiyaç bulunduğunu anlamak için, haddeleme koşulları ile merdane malzemesi bileşimlerinin ve özelliklerinin bilinmesi gerekmektedir. Bu çalışmada klasik malzemelerden, son zamanalarda geliştirilen malzemelere kadar olan gelişmenin değerlendirilmesi sunulmuş ve bi-metal merdane üretim teknolojileri irdelenmiştir. Ayrıca deneysel olarak dökümü gerçekleştirilen perlitik ve martenzitik merdane mikroyapıları incelenmiştir. Elde edilen sonuçlar ile mikroyapı oluşumlarının merdaneden beklenen özellikler üzerine etkileri analiz edilmiştir