Mach's Principle and Model for a Broken Symmetric Theory of Gravity

We investigate spontaneous symmetry breaking in a conformally invariant gravitational model. In particular, we use a conformally invariant scalar tensor theory as the vacuum sector of a gravitational model to examine the idea that gravitational coupling may be the result of a spontaneous symmetry breaking. In this model matter is taken to be coupled with a metric which is different but conformally related to the metric appearing explicitly in the vacuum sector. We show that after the spontaneous symmetry breaking the resulting theory is consistent with Mach's principle in the sense that inertial masses of particles have variable configurations in a cosmological context. Moreover, our analysis allows to construct a mechanism in which the resulting large vacuum energy density relaxes during evolution of the universe.Comment: 9 pages, no figure

De mogelijkheden van UV-C straling voor de bestrijding van ziekten en plagen in de akkerbouw : een literatuurstudie

In deze studie is op een rij gezet wat er voor de akkerbouw in de literatuur bekend is over de mogelijkheden van UV-C licht voor de bestrijding van ziekten en plagen. Het bleek dat er over de toepassing in de akkerbouw met lage doseringen UV-C licht en met korte blootstellingstijden, weinig relevante literatuur beschikbaar is. Daarom is ook naar andere literatuur gezocht om eventuele perspectieven voor de akkerbouw op te kunnen sporen. Het blijkt dat bacteriën relatief makkelijk gedood worden door UV-C straling. Het DNA van deze micro-organismen wordt snel beschadigd met minder reproductie of afsterving tot gevolg. De schimmels blijken sterk te verschillen in gevoeligheid voor UV-C. Door UV-C belichting van geoogste producten kunnen aanwezige pathogenen op de producten worden gedood (direct effect) of het product kan fysiologisch veranderen waardoor de pathogenen minder kans maken (geïnduceerde resistentie). De perspectieven voor de akkerbouw zijn beperkt

Signature of wide-spread clumping in B supergiant winds

We seek to establish additional observational signatures of the effects of clumping in OB star winds. The action of clumping on strategic wind-formed spectral lines is tested to steer the development of models for clumped winds and thus improve the reliability of mass-loss determinations for massive stars.The SiIV 1400 resonance line doublets of B0 to B5 supergiants are analysed using empirical line-synthesis models. The focus is on decoding information on wind clumping from measurements of ratios of the radial optical depths (tau_(rad)(w)) of the red and blue components of the SiIV doublet. We exploit in particular the fact that the two doublet components are decoupled and formed independently for targets with relatively low wind terminal velocities. Line-synthesis analyses reveal that the mean ratio of tau_(rad)(w) of the blue to red SiIV components are rarely close to the canonical value of ~ 2 (expected from atomic constants), and spread instead over a range of values between ~1 and 2. These results are interpreted in terms of a photosphere that is partially obscured by optically thick structures in the outflowing gas.The spectroscopic signatures established in this study demonstrate the wide-spread existence of wind clumping in B supergiants. The additional information in unsaturated doublet profiles provides a means to quantify the porosity of the winds.Comment: Accepted for publication in A&A Letter

Gene-modified T cells for adoptive immunotherapy of renal cell cancer maintain transgene-specific immune functions in vivo

Abstract BACKGROUND: We have treated three patients with carboxy-anhydrase-IX (CAIX) positive metastatic renal cell cancer (RCC) by adoptive transfer of autologous T-cells that had been gene-transduced to express a single-chain antibody-G250 chimeric receptor [scFv(G250)], and encountered liver toxicity necessitating adaptation of the treatment protocol. Here, we investigate whether or not the in vivo activity of the infused scFv(G250)(+) T cells is reflected by changes of selected immune parameters measured in peripheral blood. METHODS: ScFv(G250)-chimeric receptor-mediated functions of peripheral blood mononuclear cells (PBMC) obtained from three patients during and after treatment were compared to the same functions of scFv(G250)(+) T lymphocytes prior to infusion, and were correlated with plasma cytokine levels. RESULTS: Prior to infusion, scFv(G250)(+) T lymphocytes showed in vitro high levels of scFv(G250)-chimeric receptor-mediated functions such as killing of CAIX(+) RCC cell lines and cytokine production upon exposure to these cells. High levels of IFN-gamma were produced, whilst production of TNF-alpha, interleukin-4 (IL-4), IL-5 and IL-10 was variable and to lower levels, and that of IL-2 virtually absent. PBMC taken from patients during therapy showed lower levels of in vitro scFv(G250)-receptor-mediated functions as compared to pre-infusion, whilst IFN-gamma was the only detectable cytokine upon in vitro PBMC exposure to CAIX. During treatment, plasma levels of IFN-gamma increased only in the patient with the most prominent liver toxicity. IL-5 plasma levels increased transiently during treatment in all patients, which may have been triggered by the co-administration of IL-2. CONCLUSION: ScFv(G250)-receptor-mediated functions of the scFv(G250)(+) T lymphocytes are, by and large, preserved in vivo upon administration, and may be reflected by fluctuations in plasma IFN-gamma levels

Spectral analysis of the sdO K 648, the exciting star of the planetary nebula Ps 1 in the globular cluster M 15 (NGC 7078)

We present a spectral analysis of the sdO central star K 648 based on medium-resolution optical and high-resolution UV spectra. The photospheric parameters are determined by means of state-of-the-art NLTE model atmosphere techniques. We found Teff = 39 +/- 2 kK and log g = 3.9 +/- 0.2. The helium (He/H=0.08) and oxygen (O/H=0.001) abundances are about solar while carbon is enriched by a factor of 2.5 (C/H=0.001). Nitrogen (N/H = 10**(-6), [N/H] = -2.0) appears at a sub-solar value. However, these metal abundances are much higher than the cluster's metallicity M 15: [Fe/H] = -2.25). The surface composition appears to be a mixture of the original hydrogen-rich material and products of helium burning (3 alpha process) which have been mixed up to the surface. The abundances of He, C, and N are consistent with the nebular abundance, while O is considerably more abundant in the photosphere than in the nebula. From a comparison of its position in the log Teff - log g plane with evolutionary calculations a mass of 0.57 (+0.02, -0.01) Msun and a luminosity of 3810 +/- 1200 Lsun are deduced. Our spectroscopic distance d = 11.1 (+2.4, -2.9) kpc is in agreement with the distance of M 15 as determined by Alves et al. (2000). From the GHRS spectra we measure a radial velocity of vrad = -130 km/sec.Comment: 8 pages, 13 figure

Phosphorylation by protein kinase C and the responsiveness of Mg2+-ATPase to Ca2+ of myofibrils isolated from stunned and non-stunned porcine myocardinm

Previously we showed in an in situ porcine model that the thiadiazinone derivative [+]EMD 60263, a Ca2+ sensitizer without phosphodiesterase III inhibitory properties, increased contractility more profoundly in stunned than in non-stunned myocardium. This finding was consistent with the observed leftward shifts of the pCa2+/Mg2+-ATPase curves of isolated myofibrils induced by [+]EMD 60263. The aim of the present investigation was to study the possible involvement of protein kinase C in the mechanism of reduced Ca2+ responsiveness of myofilaments during stunning. No differences were observed in the maximal activity of the Ca2+-stimulated Mg2+-ATPase and in the pCa50 of myofibrils isolated from non-stunned and stunned myocardium. After phosphorylation with [gamma-32P]-ATP and excess of purified rat brain protein kinase C, the myofibrils were separated on sodiumdodecylsulphate-polyacrylamide gelectrophoresis and the 32P incorporation counted by the Molecular Imager. Ca2+/ phosphatidylserine/sn-1,2 diolein-dependent 32P incorporation catalyzed by excess of purified rat brain protein kinase C in C-protein, TnT and TnI subunits did not show any differences between myofibrils from non-stunned and stunned myocardium. However, protein kinase C-induced phosphorylation of myofibrils isolated from ventricular myocardium of sham-operated pigs resulted in a marked leftward shift of the PCa50 from 6.03 ± 0.04 to 6.44 ± 0.06 (p < 0.05), while porcine heart cyclic AMP-dependent protein kinase-induced phosphorylation resulted in an expected small rightward shift to 5.97, although statistical significance was not reached. Protein kinase C-induced phosphorylation also stimulated (80%) the maximal myofibrillar Mg2+-ATPase activity. [+]EMD 60263 (3 μM) produced a leftward shift of the myofibrillar pCa2+/Mg2+-ATPase curve which was unaffected by prior protein kinase C-induced phosphorylation. In conclusion, the findings with isolated myofibrils from myocardium of anaesthetized open-chest pigs indicate that protein kinase C might be involved in the mechanism of reduced Ca2+ responsiveness of myofilaments in stunned myocardium. However, at this stage no differences could be found between the maximal activity of the Ca2+-stimulated Mg2+ -ATPase, the pCa50 and the degree of phosphorylation of myofibrils isolated from stunned and non-stunned myocardium

The star cluster formation history of the LMC

The Large Magellanic Cloud is one of the nearest galaxies to us and is one of only few galaxies where the star formation history can be determined from studying resolved stellar populations. We have compiled a new catalogue of ages, luminosities and masses of LMC star clusters and used it to determine the age distribution and dissolution rate of LMC star clusters. We find that the frequency of massive clusters with masses M>5000 Msun is almost constant between 10 and 200 Myr, showing that the influence of residual gas expulsion is limited to the first 10 Myr of cluster evolution or clusters less massive than 5000 Msun. Comparing the cluster frequency in that interval with the absolute star formation rate, we find that about 15% of all stars in the LMC were formed in long-lived star clusters that survive for more than 10 Myr. We also find that the mass function of LMC clusters younger than 1 Gyr can be fitted by a power-law mass function with slope \alpha=-2.3, while older clusters follow a significantly shallower slope and interpret this is a sign of the ongoing dissolution of low-mass clusters. Our data shows that for ages older than 200 Myr, about 90% of all clusters are lost per dex of lifetime. The implied cluster dissolution rate is significantly faster than that based on analytic estimates and N-body simulations. Our cluster age data finally shows evidence for a burst in cluster formation about 1 Gyr ago, but little evidence for bursts at other ages.Comment: 18 pages, 6 figures, MNRAS in pres

In vitro analysis of SERCA2 gene regulation in hypertrophic cardiomyocytes and increasing transfection efficiency by gene-gun biolistics

The transcriptional downregulation of the SERCA2 gene is studied using neonatal rat cardiomyocytes stimulated with endothelin-1 to induce hypertrophy. Liposome-based transfection of cells with a 1.9 kb SERCA2 promoter fragment directed expression of a reporter gene identical to the downregulation of genomic SERCA2 expression by endothelin-1. Results of a new gene gun technology for transient transfection of cardiomyocytes with a RSVβ-galactosidase construct are reported. This new method for propelling DNA-coated gold beads into cardiomyocytes is extremely suitable for directly testing promoter/reporter gene DNA constructs since the transfection efficiency (approximately 10%) appears to be higher than traditional transfection methods

Properties of galactic B[e] supergiants. IV. Hen3-298 and Hen3-303

We present the results of optical and near-IR spectroscopic and near-IR photometric observations of the emission-line stars Hen3-298 and Hen3-303. Strong emission in the H-alpha line is found in both objects. The presence of Fe II and [O I] emission lines in the spectrum of Hen3-298 indicates that it is a B[e] star. The double-peaked CO line profiles, found in the infrared spectrum of Hen3-298, along with the optical line profiles suggest that the star is surrounded by a rotating circumstellar disk. Both objects also show infrared excesses, similar to those of B[e] stars. The radial velocities of the absorption and emission lines as well as a high reddening level suggest that the objects are located in the Norma spiral arm at a distance of 3-4.5 kpc. We estimated a luminosity of log (L/L_sun) ~ 5.1 and a spectral type of no earlier than B3 for Hen3-298. Hen3-303 seems to be a less luminous B-type object (log (L/L_sun) ~ 4.3), located in the same spiral arm.Comment: 8 pages, 5 figures, accepted by Astronomy and Astrophysic