Attraction of Culex mosquitoes to aldehydes from human emanations.

Anecdotes related to preferential mosquito bites are very common, but to date there is no complete explanation as to why one out of two people systematically receives more mosquito bites than the other when both are equally accessible. Here we tested the hypothesis that two constituents of skin emanations, 6-methyl-5-heptan-2-one (6-MHO) and geranylacetone (GA), are natural repellents and may account for differential attraction in different ratios. We studied skin emanations from two human subjects, confirmed in behavioral assays that female southern house mosquitoes are significantly more attracted to subject A (attractant) than to subject N (non-attractant), and tested their 6-MHO/GA ratios in a dual-choice olfactometer. Although repelling at high doses, 6-MHO/GA mixtures were not active at the levels emitted by human skin. We found, however, differential attraction elicited by the aldehydes in the ratios produced by subjects A and N. When tested in a dose commensurate with the level released from human skin and in the ratio produced by subject A, the aldehyde mixture significantly attracted mosquitoes. By contrast, an aldehyde mixture at the same ratio released by subject N did not attract mosquitoes. We, therefore, hypothesized that aldehydes may play a role in the commonly observed differential attraction

Peat’s secret archive: Interpreting the geochemical and palaeodust record from Scottish peat as a potential index of North Atlantic storminess and Holocene climate change

Four continuous high-resolution peat records for the Holocene have been reconstructed across a ~300km transect from Shebster in Caithness to Yell in the Shetland Isles. These records describe the nature and extent of North Atlantic climate changes inferred from indicators of storminess and minerogenic aeolian dust, and are supported by radiogenic isotope analysis, tephrochronology and radiocarbon dating. The environmental changes at all four sites displays a significant degree of synchrony in response to changes in the position of the polar front jet (PFJ) stream and the phase of the North Atlantic Oscillation (NAO). Bromine concentrations in the peat, derived from sea spray, are used to reconstruct storm frequency and storm intensity, and mire surface wetness is used as an indicator of longer-term climate shifts. The results suggest a strong link between positive phases of the NAO and storminess. Subtle differences between the bromine concentrations and the mire surface wetness suggest that high intensity but perhaps less frequent periods of storminess are not necessarily associated with a wetter climate. Atmospheric minerogenic dust concentrations are used to reconstruct large-scale climate changes across the wider North Atlantic region. The results suggest a sympathy between dust activity and periods of glacial advance and a negative index of the NAO. Radiogenic isotope analysis suggests that the smallest particles may originate from Iceland

Temporal trend in the transfer of Sellafield-derived 14C into different size fractions of the carbonate component of NE Irish Sea sediment

From 1994 onwards, 14C discharges from the Sellafield nuclear fuel reprocessing plant have been made largely to the Northeast Irish Sea. They represent the largest contributor to UK and European populations of the collective dose commitment derived from the entire nuclear industry discharges. Consequently, it is important to understand the long-term fate of 14C in the marine environment. Research undertaken in 2000 suggested that the carbonate component of Northeast Irish Sea sediments would increase in 14C activity as mollusc shells, which have become enriched in Sellafield-derived 14C, are broken down by physical processes including wave action and incorporated into intertidal and sub-tidal sediments. The current study, undertaken in 2011, tested this hypothesis. The results demonstrate significant increases in 14C enrichments found in whole mussel shells compared to those measured in 2000. Additionally, in 2000, there was an enrichment above ambient background within only the largest size fraction (>500 μm) of the intertidal inorganic sediment at Nethertown and Flimby (north of Sellafield). In comparison, the present study has demonstrated 14C enrichments above ambient background in most size fractions at sites up to 40 km north of Sellafield, confirming the hypothesis set out more than a decade ago

Gastropod-derived haemocyte extracellular traps entrap metastrongyloid larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior

Background: Phagocyte-derived extracellular traps (ETs) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. In the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (InEPT) formation in response to larval stages of important canine and feline metastrongyloid lungworms. Gastropod haemocytes were isolated from the slug species Arion lusitanicus and Limax maximus, and the snail Achatina fulica, and exposed to larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior and investigated for gastropod-derived InEPT formation. Results: Phase contrast as well as scanning electron microscopy (SEM) analyses of lungworm larvae-exposed haemocytes revealed ET-like structures to be extruded by haemocytes thereby contacting and ensnaring the parasites. Co-localization studies of haemocyte-derived extracellular DNA with histones and myeloperoxidase in larvae-entrapping structures confirmed classical characteristics of ETs. In vivo exposure of slugs to A. vasorum larvae resulted in InEPTs being extruded from haemocytes in the slug mucous extrapallial space emphasizing the pivotal role of this effector mechanism against invasive larvae. Functional larval entrapment assays demonstrated that almost half of the haemocyte-exposed larvae were contacted or even immobilized by released InEPTs. Overall, as reported for mammalian-derived ETs, different types of InEPTs were here observed, i.e. aggregated, spread and diffused InEPTs. Conclusions: To our knowledge, this study represents the first report on metastrongyloid lungworm-triggered ETosis in gastropods thereby providing evidence of early mollusc host innate immune reactions against invading larvae. These findings will contribute to the better understanding on complex parasite-intermediate host interactions since different gastropod species bear different transmitting capacities for metastrongyloid infections

Comparison of Acidic and Basic Chromosomal Proteins from Normal Human Endometrium and Undifferentiated Endometrial Carcinoma by Isoelectric Focussing and Microgel-Electrophoresis

Peer Reviewe

Enantioselective PCCP Brønsted acid-catalyzed aza-Piancatelli rearrangement.

An enantioselective aza-Piancatelli rearrangement has been developed using a chiral Brønsted acid based on pentacarboxycyclopentadiene (PCCP). This reaction provides rapid access to valuable chiral 4-amino-2-cyclopentenone building blocks from readily available starting material and is operationally simple

Evaluation of seatbelt use among pregnant women in Sweden

A digital questionnaire was distributed through social media targeting women who were or had been pregnant.The primary objective was to investigate self-reported seatbelt use and misuse during pregnancy. The secondobjective was to study if, and to which extent, women had received information regarding seatbelt use and howto wear it during pregnancy.The survey of 2,030 women who were or had been pregnant showed a total seatbelt wearing rate of 99%.However, 39% were wearing the seatbelt wrongly. In 35% of cases, the shoulder section of the seatbelt wasincorrectly positioned, and the lap section of the seatbelt in 8% of cases. In 4% of cases, both the shoulder andlap belt parts of the seatbelt were incorrectly positioned.The majority (66%) had not received any information regarding proper seatbelt use during pregnancy. Of the 700women (34%) who had received information, most of them had actively sought out information about how theseatbelt should be worn during pregnancy. This subgroup had significantly lower misuse rate, although a third ofthe group wore the seatbelt incorrectly. Very few (6%), had received information via a health care provider.Present data highlight the need for improved seatbelt fit for pregnant women. The result from the survey showsthat misuse was lower among the women whom actively searched for information regarding how to wear theseatbelt. To reach other user groups, it should be a priority for several stakeholders to communicate informationregarding proper seatbelt use during pregnancy

Domoic Acid-Induced Neurotoxicity Is Mainly Mediated by the AMPA/KA Receptor: Comparison between Immature and Mature Primary Cultures of Neurons and Glial Cells from Rat Cerebellum

Domoic acid (DomA) is a naturally occurring shellfish toxin that can induce brain damage in mammalians. Neonates have shown increased sensitivity to DomA-induced toxicity, and prenatal exposure has been associated with e.g. decreased brain GABA levels, and increased glutamate levels. Here, we evaluated DomA-induced toxicity in immature and mature primary cultures of neurons and glial cells from rat cerebellum by measuring the mRNA levels of selected genes. Moreover, we assessed if the induced toxicity was mediated by the activation of the AMPA/KA and/or the NMDA receptor. The expression of all studied neuronal markers was affected after DomA exposure in both immature and mature cultures. However, the mature cultures seemed to be more sensitive to the treatment, as the effects were observed at lower concentrations and at earlier time points than for the immature cultures. The DomA effects were completely prevented by the antagonist of the AMPA/KA receptor (NBQX), while the antagonist of the NMDA receptor (APV) partly blocked the DomA-induced effects. Interestingly, the DomA-induced effect was also partly prevented by the neurotransmitter GABA. DomA exposure also affected the mRNA levels of the astrocytic markers in mature cultures. These DomA-induced effects were reduced by the addition of NBQX, APV, and GABA