Оптимізація макрологістичних мереж

We report on a Laser source, emitting in the mid-IR spectral range. A mode-locked Nd: YVO Laser is used as a pump Laser, followed by two nonlinear frequency conversion steps (OPG, DFG). The broad-band output beam is tunable between 9 ?m and 13 ?m, with average powers up to 10 mW. Further extension of the tuning range is possible

Врахування вітчизняних умов при використанні закордонних Z-моделей прогнозування банкрутства

The green cw laser presented in this work is realized by means of a Pr:YLF crystal emitting at 523 nm that is pumped by a blue GaN laser diode in an extremely short resonator. With a 500 mW-diode a laser has been achieved with M2 = 1, a slope of 40 % and an output power of 140mW with an absorbed pump power of 410 mW which results in an electrooptical efficiency of 6.5 %. Despite the reduced overlap with a 1 W-diode the output power rises to 290 mW with an absorbed pump power of 850 mW and the M2 increases only slightly. Based on these results a compact laser package has been accomplished using a monolithic micro optics for the beam shaping of the diode light and joining all components with a low-shrinkage adhesive on a common base plate. In a first test of the alignment strategy a laser with an output power of 92 mW has been achieved by means of the 500 mW pump power

Stabilizing sodium hypochlorite at high pH: effects on soft tissue and dentin

NaOH-stabilized NaOCl solutions have a higher alkaline capacity and are thus more proteolytic than standard counterparts

Исследование влияния температуры на процесс восстановления ацетилдифенила изопропилатом алюминия

We present a compact module, emitting nearly diffraction limited green laser light at 531 nm at an average output power of more than 500 mW. As pump source for the second harmonic generation a DBR tapered laser with a total length of 6 mm was used. The RW section had a length of 2 mm including a 1 mm long passive DBR section. The devices were mounted p-side up on a copper block. For this mounting scheme, the device reaches up to 7 W maximal output power. At the power level of about 3.8 W used in the presented experiment, a wavelength of 1062.6 nm with a line-width below 0.02 nm (FWHM) was determined. More than 80% of the emitted power is originated within the central lobe of the beam waist profile illustrating the nearly diffraction limited beam quality. Using a 30mm long MgO-doped periodically poled LiNbO3 bulk crystal, the second harmonic wave is generated in a single-pass setup. Due to precise alignment and beam shaping based on the results of numerical simulations and a properly temperature control of the PPLN crystal, a maximum optical conversion efficiency of more than 14% (3.7%/W) was achieved. The fluctuation of the output power is far below 1%

Fixed bobbin friction stir welding of marine grade aluminium.

PROBLEM - The bobbin friction stir welding (BFSW) process has potential benefits for welding thin sheet aluminium alloy. The main benefits of friction stir welding over conventional thermal welding processes are minimisation of energy usage, no need for consumables, potential for good weld quality without porosity, no fumes, minimal adverse environmental effects (green), minimal waste (lean), and reduced threats to personal health and safety. The BFSW process has further advantages over conventional friction stir welding (CFSW) in the reduction of welding forces, faster welding, and less fixturing. It is especially attractive to industries that join thin sheet material, e.g. boat-building. The industrial need for this project arose from the desire to apply the technology at a ship manufacturing company, INCAT located in Hobart, Tasmania, Australia. However there are peculiar difficulties with the specific grade of material used in this industry, namely thin sheet aluminium Al6082-T6. Early efforts with a portable friction stir welding machine identified the process to have low repeatability and reproducibility, i.e. process-instability. There are a large number of process variables and situational factors that affect weld quality, and many of these are covert. This is also the reason for divergent recommendations in the literature for process settings. PURPOSE - The main purpose of this research was to identify covert variables and better understand their potentially adverse effects on weld quality. Therefore, this thesis investigated the hidden variables and their interactions. Developing this knowledge is a necessity for making reliable and repeatable welds for industrial application. APPROACH - An explorative approach that focused on the functional perspective was taken. An extensive empirical testing programme was undertaken to identify the variables and their effects. In the process a force platform and BFSW tools were designed and built. A variety of machine platforms were used, namely portable friction stir welding, manual milling machine and computer numerical control (CNC) milling machine. The trials were grouped into 14 test plans. These are tool shoulder gap, spindle and travel speed, tool features, machines, tool fixation, machinery, welding direction, plate size (width and dimension), support insulation, tool materials, substrate properties and fixation. For the welded plates besides visual inspection of the weld, current, force, and temperature were measured. The Fourier transform was used to analyse the frequency response of machines. Also the welded samples were tested to the maritime standards of Det Norske Veritas (DNV). A number of relationships of causality were identified whereby certain variables affected weld quality. A model was developed to represent the proposed causality using the IDEF0 systems engineering method. FINDINGS - From these trials six main variables have been identified. These are tool features, spindle speed, travel speed, shoulder gap compression, machine variability, tool and substrate fixation. A rigid system is required for a consistent weld results. Under this condition, full pin features (threads and flats) need to be used to balance the adverse effects of individual features. It has been shown that fabricated bobbin tools with sharp edges can cause cuts and digging thus this feature should be avoided. Additionally, the substrate should have continuous interaction with the tool so the shoulder interference needs to be fixed and well-controlled. It is found that the compression generated by the shoulder towards the substrate helps material grabbing for better tool-substrate interaction. It is also shown that tool entry causes ejection of material and hence an enduring mass deficit, which manifests as a characteristic tunnel defect. The new explanation of the formation, origin and location of this defect has been explained. Material transportation mechanisms within the weld have been elucidated. It is also found that the role of the travel speed is not only to control heat generation but also for replacing the deficit material. Additionally, heat supplied to the weld depends not only on thickness, but also the width of the plate. Different types of machine cause an interaction in the material flow through their controller strategies. Jerking motion can occur at a slow travel speed, which also alters the way material is being transported. The Fourier transform (FFT) has been used to identify the characteristics of good and bad BFSW welds. This has the potential to be expanded for real-time process control. IMPLICATIONS - Tool deflection and positioning, material flow and availability are identified as affecting weld quality through stated mechanisms. The impact is even more severe when involving thin-plate aluminium. For the industry to successfully adopt this technology the process typically needs tight control of shoulder gap, tool strength and stiffness, feature fabrication, substrate and tool fixation. Additionally spindle and travel speed need to be adjusted not only based on the type of materials and thickness, but also the width, type of machine and method of tool entry. ORIGINALITY - New data are presented, which lead to new insights into the welding mechanics, production settings, material transportation and weld defects for BFSW on thin sheet material. The conventional idea that the welding tool has a semi-steady interaction with the substrate is not supported. Instead the interaction is highly dynamic, and this materially affects the weld-quality, especially in the difficult-to-weld material under examination. Factors such as shoulder gap, tool and substrate fixation compliance and machine types emerge as variables that need to be given attention in the selection of process parameters. The causal relationships have been represented in a conceptual model using an IDEF0 system approach. This study has made several original contributions to the body of knowledge. First is the identification of previously hidden variables that effect weld formation for the fixed gap BFSW process. The second contribution is a new way of understanding the material transportation mechanics within the weld. This includes the flow around the pin in the plane of the weld, the vertical transportation of material up the pin, the formation of turbulent-like knit lines at the advancing side, and the formation of tunnel defects. Also included here is a new understanding of how material deficit arises at tool entry and exit, and from flash/chips, and how this contributes to the tunnel weld defect. In addition, new understandings of the role of feed rate have been identified. Related to the material transportation, the work has also identified the importance of an interference fit between the substrate and tool. A third contribution is the identification of the dynamic interaction between tool and substrate. This identifies the important role rigidity plays. Associated with this is the identification of frequency characteristics of the motors under load. The fourth contribution is identification of the specific process settings for the difficult-to-weld material of AL6082-T6. The fifth contribution is the development of a novel method of fabricating bobbin friction stir welding tools as embodied in a patent application

Multi-minicore Disease

Multi-minicore Disease (MmD) is a recessively inherited neuromuscular disorder characterized by multiple cores on muscle biopsy and clinical features of a congenital myopathy. Prevalence is unknown. Marked clinical variability corresponds to genetic heterogeneity: the most instantly recognizable classic phenotype characterized by spinal rigidity, early scoliosis and respiratory impairment is due to recessive mutations in the selenoprotein N (SEPN1) gene, whereas recessive mutations in the skeletal muscle ryanodine receptor (RYR1) gene have been associated with a wider range of clinical features comprising external ophthalmoplegia, distal weakness and wasting or predominant hip girdle involvement resembling central core disease (CCD). In the latter forms, there may also be a histopathologic continuum with CCD due to dominant RYR1 mutations, reflecting the common genetic background. Pathogenetic mechanisms of RYR1-related MmD are currently not well understood, but likely to involve altered excitability and/or changes in calcium homeoestasis; calcium-binding motifs within the selenoprotein N protein also suggest a possible role in calcium handling. The diagnosis of MmD is based on the presence of suggestive clinical features and multiple cores on muscle biopsy; muscle MRI may aid genetic testing as patterns of selective muscle involvement are distinct depending on the genetic background. Mutational analysis of the RYR1 or the SEPN1 gene may provide genetic confirmation of the diagnosis. Management is mainly supportive and has to address the risk of marked respiratory impairment in SEPN1-related MmD and the possibility of malignant hyperthermia susceptibility in RYR1-related forms. In the majority of patients, weakness is static or only slowly progressive, with the degree of respiratory impairment being the most important prognostic factor

Gain-switched all-fiber laser with narrow bandwidth

Gain-switching of a CW fiber laser is a simple and cost-effective approach to generate pulses using an all-fiber system. We report on the construction of a narrow bandwidth (below 0.1 nm) gain-switched fiber laser and optimize the pulse energy and pulse duration under this constraint. The extracted pulse energy is 20 jiJ in a duration of 135 ns at 7 kHz. The bandwidth increases for a higher pump pulse energy and repetition rate, and this sets the limit of the output pulse energy. A single power amplifier is added to raise the peak power to the kW-level and the pulse energy to 230 ßJ while keeping the bandwidth below 0.1 nm. This allows frequency doubling in a periodically poled lithium tantalate crystal with a reasonable conversion efficiency

Altered splicing of the BIN1 muscle-specific exon in humans and dogs with highly progressive centronuclear myopathy

Amphiphysin 2, encoded by BIN1, is a key factor for membrane sensing and remodelling in different cell types. Homozygous BIN1 mutations in ubiquitously expressed exons are associated with autosomal recessive centronuclear myopathy (CNM), a mildly progressive muscle disorder typically showing abnormal nuclear centralization on biopsies. In addition, misregulation of BIN1 splicing partially accounts for the muscle defects in myotonic dystrophy (DM). However, the muscle-specific function of amphiphysin 2 and its pathogenicity in both muscle disorders are not well understood. In this study we identified and characterized the first mutation affecting the splicing of the muscle-specific BIN1 exon 11 in a consanguineous family with rapidly progressive and ultimately fatal centronuclear myopathy. In parallel, we discovered a mutation in the same BIN1 exon 11 acceptor splice site as the genetic cause of the canine Inherited Myopathy of Great Danes (IMGD). Analysis of RNA from patient muscle demonstrated complete skipping of exon 11 and BIN1 constructs without exon 11 were unable to promote membrane tubulation in differentiated myotubes. Comparative immunofluorescence and ultrastructural analyses of patient and canine biopsies revealed common structural defects, emphasizing the importance of amphiphysin 2 in membrane remodelling and maintenance of the skeletal muscle triad. Our data demonstrate that the alteration of the muscle-specific function of amphiphysin 2 is a common pathomechanism for centronuclear myopathy, myotonic dystrophy, and IMGD. The IMGD dog is the first faithful model for human BIN1-related CNM and represents a mammalian model available for preclinical trials of potential therapies

An overview of cancer/testis antigens expression in classical Hodgkin's lymphoma (cHL) identifies MAGE-A family and MAGE-C1 as the most frequently expressed antigens in a set of Brazilian cHL patients

<p>Abstract</p> <p>Background</p> <p>Cancer/testis antigens are considered potential targets for immunotherapy due to their tumor-associated expression pattern. Although recent studies have demonstrated high expression of CT45 in classical Hodgkin's lymphomas (cHL), less is known about the expression pattern of other families of CTAs in cHL. We aim to evaluate the expression of MAGE-A family, MAGE-C1/CT7, MAGE-C2/CT10, NY-ESO1 and GAGE family in cHL and to correlate their expression with clinical and prognostic factors in cHL.</p> <p>Methods</p> <p>Tissue microarray was generated from 38 cHL archival cases from Pathology Department of Universidade Federal de Sao Paulo. Immunohistochemistry (IHC) was done using the following panel of antibodies: MAGE-A family (MA454, M3H67, 57B and 6C1), GAGE (#26), NY-ESO-1 (E978), MAGE-C1/CT7 (CT7-33) and MAGE-C2/CT10 (CT10#5).</p> <p>Results</p> <p>We found CTA expression in 21.1% of our cHL series. Among the tested CTAs, only MAGE-A family 7/38 (18.4%) and MAGE-C1/CT7 5/38 (13.2%) were positive in our cHL samples. We found higher CTA positivity in advanced stage (28.6%) compared to early stage (11.8%) disease, but this difference was not statistically significant. Analysis of other clinicopathological subgroups of cHL including histological subtypes, EBV status and response to treatment also did not demonstrate statistical significant differences in CTA expression.</p> <p>Conclusion</p> <p>We found CTA expression in 21.1% of cHL samples using our panel. Our preliminary findings suggest that from all CTAs included in this study, MAGE-A family and MAGE-C1/CT7 are the most interesting ones to be explored in further studies.</p