Musicando

Anais do I Encontro de Iniciação Científica e de Extensão da Unila – Extensão: Grupo temático “Tons e Sons da América Latina” - 05/06/12 - 08h00 às 18h00 - Unila-Centro - Salas 10 e 11 - 2o PisoEl proyecto Musicando se desarrolló en torno al conocimiento y la difusión de ritmos latinoamericanos (tango, candombe, samba, cuarteto, joropo, bolero, entre otros) entre la comunidad académica de Unila. Constó de reuniones temáticas semanales realizadas en el PTI. Estas fueron de carácter expositivo (a través de información, música y videos) y de debate. Llevadas a cabo, de forma alternada, por la profesora y los bolsistas. También fueron introducidos algunos conceptos músicales como: timbre, altura, intensidad. Estos sirvieron para generar un mayor acercamiento y sensibilidad al material sonoro.Universidade Federal da Integração Latino-Americana (UNILA

Age estimation of Russian individuals by teeth using the London Atlas

Conhecer a idade de um indivíduo, vivo ou morto, é essencial em diversas situações. O método London Atlas utiliza desenvolvimento e erupção dentais para estimar a idade. Testar o método em diferentes populações é importante para avaliar sua performance. Esse estudo objetivou analisar o desempenho do London Atlas em uma amostra de origem russa, utilizando radiografias panorâmicas. Uma amostra de 703 indivíduos russos (n = 405 mulheres, 57,61% e n = 298 homens, 42,39%), com idades entre 8 e 23 anos foram analisadas. Resultados obtidos mostram uma superestimação em indivíduos de 8 a 14 anos e subestimação nos grupos de 15 a 23 anos. A diferença média entre idades estimadas e reais não excederam o valor de 0,7 anos nos indivíduos com idade abaixo de 19 anos. Essa diferença aumentou em até três anos em indivíduos de 20 a 23 anos. Diferenças estatisticamente significantes foram encontradas entre homens e mulheres com 17 e 18 anos (p<0,05). O London Atlas é adequado para crianças e adolescentes de origem russa, com idades de 8 a 19 anos. No entanto, observou-se resultados insatisfatórios para sua aplicação em indivíduos acima de 20 anos.Knowing an individual's age is necessary for several situations, both in the living and the deceased. The London Atlas uses dental development and eruption to estimate age. Testing the method in different populations is necessary to assess its performance. This study aimed to assess the performance of the London Atlas method in a Russian sample using panoramic radiographs. A sample of 703 panoramic radiographs of Russian individuals (n = 405 females, 57.61% and n = 298 males, 42.39%) with ages between 8 and 23 years were analyzed. The results showed overestimation in individuals from 8 to 14 years and underestimation from 15 to 23 years. The mean difference between estimated and chronological ages did not exceed 0.7 years among individuals with ages below 19 years. The difference increased to over three years in individuals from 20 to 23 years. Statistically significant differences were found between females and males between 17 and 18 years (p<0.05). The London Atlas is suitable for Russian children and adolescents aged between 8 and 19 years; however, it showed unsatisfactory results for application in individuals over 20 years

Ciliate Communities Respond via Their Traits to a Wastewater Treatment Plant With a Combined UASB-Activated Sludge System

Assessing functional diversity of communities is an efficient method to link community composition to ecosystem quality. Still, studies using functional traits of microeukaryote ciliate communities in biological wastewater treatment plants are lacking. The present work explores the functional diversity of the ciliate protist community in a wastewater treatment plant (WWTP) operating with a combined UASB-activated sludge system, and specifically to: 1) investigate the taxonomic and functional composition of the ciliate communities over time; 2) compare taxonomic and functional diversity indices with regard to its applicability in WWPS; 3) assess the relationship between the ciliate community's functional composition and the WWTPs temporal conditions; and 4) investigate the potential use of functional diversity as an indicator of WWTP efficiency. Totally, we recorded 21 ciliate species throughout 37 samplings. The number of species was low compared to other plants. Bacterivorous and flake-forming species were the main functional strategies found in the samples. The correlation between taxonomic and functional richness was significant, indicating a functionally redundant community. There was a correlation between the Simpson and Rao's quadratic entropy indexes suggesting that loss of taxonomic diversity leads to a loss of functional diversity. The homogeneity of the measured physical and chemical data led to functional homogenization and redundancy (homogenous CWM) of the ciliate community. The functional diversity is positively correlated with parameters of removal efficiency, indicating a promising application in WWTPs. Future studies will broaden knowledge on functional diversity in biological wastewater treatment systems, this being a first step with the unprecedented application of this methodology in artificial ecosystems.Peer reviewe

Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content

Physical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other organs communicate to translate exercise into health. Low, moderate and high intensity treadmill protocols were applied to rat groups, aiming to investigate the impact of exercise on serum EVs and their associated small RNA molecules. Transmission electron microscopy, resistive pulse sensing, and western blotting were used to investigate EVs morphology, size distribution, concentration and EVs marker proteins. Small RNA libraries from EVs RNA were sequenced. Exercise did not change EVs size, while increased EVs concentration. Twelve miRNAs were found differentially expressed after exercise: rno-miR-128-3p, 1033p, 330-5p, 148a-3p, 191a-5p, 10b-5p, 93-5p, 25-3p, 142-5p, 3068-3p, 142-3p, and 410-3p. No piRNA was found differentially expressed, and one tRNA, trna8336, was found down-regulated after exercise. The differentially expressed miRNAs were predicted to target genes involved in the MAPK pathway. A single bout of exercise impacts EVs and their small RNA load, reinforcing the need for a more detailed investigation into EVs and their load as mediators of health-promoting exercise

Antifungal nanofibers made by controlled release of sea animal derived peptide

© The Royal Society of Chemistry 2015Candida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite the clear difficulties of Candida control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for fungal control. In this report, different concentrations of antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers for drug delivery. The nanofibers were characterized by mass spectrometry confirming the presence of the peptide on the scaffold. Atomic force microscopy and scanning electronic microscopy were used to measure the diameters, showing that Cm-p1 affects fiber morphology as well as the diameter and scaffold thickness. The Cm-p1 release behavior from the nanofibers demonstrated peptide release from 30 min to three days, leading to effective yeast control in the first 24 hours. Moreover, the biocompatibility of the fibers were evaluated through a MTS assay as well as ROS production by using a HUVEC model, showing that the fibers do not affect cell viability and only nanofibers containing 10% Cm-p1–PVA improved ROS generation. In addition, the secretion of pro-inflammatory cytokines IL-6 and TNF-α by the HUVECs was also slightly modified by the 10% Cm-p1–PVA nanofibers. In conclusion, the electrospinning technique applied here allowed for the manufacture of biodegradable biomimetic nanofibrous extracellular membranes with the ability to control fungal infectionThis work was supported by CAPES, FUNDECT, CNPq, FAPDF, UCB, Fundação para a Ciência e Tecnologia – Ministério da Educação e Ciência (FCT-MEC, Portugal) and the Calouste Gulbenkian Foundation (Portugal

INVESTIGAÇÃO DA OCORRÊNCIA DE REAÇÕES ADVERSAS COM USO DE COLEIRA IMPREGNADA POR DELTAMETRINA A 4% EM CÃES

A deltametrina é um inseticida do grupo dos piretróides tipo II muito utilizada como ectoparasiticida na veterinária em várias formas de aplicação tópica, como banho, spray, solução para aplicação no dorso e coleira. A coleira impregnada com deltametrina a 4% (CD4%), além de sua indicação contra carrapatos, tem sido recomendada também contra as picadas dos flebótomos transmissores da Leishmaniose. Alguns relatos têm sido descritos de ingestão e intoxicação, além de reações cutâneas com o uso da CD4%, porém, até o presente momento não há um estudo controlado sobre a possibilidade destes eventos em cães. O objetivo deste trabalho foi investigar em cães a ocorrência de reações adversas à CD4%. Foram utilizados 33 cães mantidos em baias coletivas do canil da UNOESTE, divididos em 3 grupos com 11 animais cada, sendo o grupo A de fêmeas adultas (utilização da coleira conforme a indicação do fabricante), grupo B de machos adultos (utilização da coleira não seguindo a indicação do fabricante) e grupo C de fêmeas jovens (utilização da coleira conforme a indicação do fabricante, porém com um período de adaptação). Os animais foram observados com relação às possíveis reações adversas ou alérgicas com preenchimento de ficha clínica e dermatológica diariamente durante 15 dias e depois três vezes por semana até completar 4 meses, tempo recomendado de permanência da coleira com eficácia pelo fabricante. Dos 33 cães, 39,4% (13) retiram a coleira, sendo 9 fêmeas (3 do grupo A e 6 do grupo C) e 4 machos (grupo B). Dos 13 animais que retiram a coleira, 18,2% ingeriram a mesma, 4 fêmeas (grupo C) e 2 machos (grupo B). Nenhum animal apresentou reação alérgica ou adversa à coleira, inclusive naqueles que ingeriram a mesma. Não houve a ocorrência de ectoparasitas durante o período de observação. Podemos concluir que a CD4% é segura e eficiente no controle de ectoparasitas e que as recomendações do fabricante são adequadas para o uso da mesma não havendo necessidade de período de adaptação. A aglomeração de cães, principalmente jovens, pode aumentar a incidência da retirada da coleira