Nuevos registros de escafópodos para las costas de Jalisco y Colima, México

The distribution of two species of scaphopods is extended south to the coast of Jalisco and Colima, México. Samples were taken with a Van Veen grab at depths between 41 and 75 m in medium sand, sandy silt and silty clay substrates. These species are: 1) Dentalium neohexagonum Sharp and Pilsbry, 1897, previouslyreported from Monterey Bay, California to Tiburón Island in the Gulf of California at depths of 7-256 m; it was collected in Jalisco at 72-75 m in sandy silt and silty clay; 2) Gadila fusiformis Pilsbry and Sharp,1898, recorded in Monterey, California, in San Carlos, Sonora and in Cabo San Lucas and the Gulf of California at depths of 7-365 m; it was collected in Colima and Jalisco, at 41 and 73 m in medium sand and silty clay substrata, respectively. The vertical distribution of another scaphopod species is also extended: Cadulus albicomatus Dall, 1890, previously registered between 733 and 3-050 m, it was collected in three localities at depths of 41, 48 and 73 m. The abundance and the frequency of occurrence of these species in the sampling stations is presented.Se informa sobre la ampliación del ámbito de distribución sur de dos especies de escafópodos hasta las costas de Jalisco y Colima, México. Las muestras fueron obtenidas con una draga Van Veen a profundidades entre 41 y 75 m en sustratos de tipo arena media, limo arenoso y limo arcilloso. Estas especies son: 1) Dentalium neohexagonum Sharp y Pilsbry, 1897, anteriormente con límites de distribución desde Bahía Monterey, California hasta Isla Tiburón, Golfo de California a profundidades de 7-256 m; se recolectó en tres localidades de la costa de Jalisco a profundidades de 72- 75 m en sustratos limo arenoso y limo arcilloso; 2) Gadila fusiformis Pilsbry y Sharp, 1898, antes registrada en Monterey, California, enSan Carlos, Sonora y en Cabo San Lucas y el Golfo de California a profundidades de 7-365 m; se recolectó en dos localidades de Colima y Jalisco, a profundidades de 41 y 73 m en sustratos de arena media y limo arcilloso, respectivamente. Además, se amplía la distribución vertical de Cadulus albicomatus Dall, 1890, anteriormente registrada entre los 733-3050 m, fue recolectada en tres localidades a 41, 48 y 73 m de profundidad. Se presentan valores de abundancia y frecuencia relativa en las estaciones para todas estas especies

Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA

The screening of the BCR::ABL1 kinase domain (KD) mutation has become a routine analysis in case of warning/failure for chronic myeloid leukemia (CML) and B-cell precursor acute lymphoblastic leukemia (ALL) Philadelphia (Ph)-positive patients. In this study, we present a novel DNA-based next-generation sequencing (NGS) methodology for KD ABL1 mutation detection and monitoring with a 1.0E−4 sensitivity. This approach was validated with a well-stablished RNA-based nested NGS method. The correlation of both techniques for the quantification of ABL1 mutations was high (Pearson r = 0.858, p < 0.001), offering DNA-DeepNGS a sensitivity of 92% and specificity of 82%. The clinical impact was studied in a cohort of 129 patients (n = 67 for CML and n = 62 for B-ALL patients). A total of 162 samples (n = 86 CML and n = 76 B-ALL) were studied. Of them, 27 out of 86 harbored mutations (6 in warning and 21 in failure) for CML, and 13 out of 76 (2 diagnostic and 11 relapse samples) did in B-ALL patients. In addition, in four cases were detected mutation despite BCR::ABL1 < 1%. In conclusion, we were able to detect KD ABL1 mutations with a 1.0E−4 sensitivity by NGS using DNA as starting material even in patients with low levels of disease.Tis project was funded in part by CRIS CANCER FOUNDATION

Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA

The screening of the BCR::ABL1 kinase domain (KD) mutation has become a routine analysis in case of warning/failure for chronic myeloid leukemia (CML) and B-cell precursor acute lymphoblastic leukemia (ALL) Philadelphia (Ph)-positive patients. In this study, we present a novel DNA-based next-generation sequencing (NGS) methodology for KD ABL1 mutation detection and monitoring with a 1.0E-4 sensitivity. This approach was validated with a well-stablished RNA-based nested NGS method. The correlation of both techniques for the quantification of ABL1 mutations was high (Pearson r = 0.858, p < 0.001), offering DNA-DeepNGS a sensitivity of 92% and specificity of 82%. The clinical impact was studied in a cohort of 129 patients (n = 67 for CML and n = 62 for B-ALL patients). A total of 162 samples (n = 86 CML and n = 76 B-ALL) were studied. Of them, 27 out of 86 harbored mutations (6 in warning and 21 in failure) for CML, and 13 out of 76 (2 diagnostic and 11 relapse samples) did in B-ALL patients. In addition, in four cases were detected mutation despite BCR::ABL1 < 1%. In conclusion, we were able to detect KD ABL1 mutations with a 1.0E-4 sensitivity by NGS using DNA as starting material even in patients with low levels of disease

Kwapa: Gente del río. Estrategias transmedia de impacto social

El PAP Alter Código, período Primavera 2023, trabajó con los dos proyectos que se han venido trabajando en semestres anteriores: el videojuego A Orillas del río y el documental Déjennos pescar. Ambos proyectos parten de la metodología interdisciplinaria y colaborativa con miembros de la comunidad Cucapá para crear representaciones audiovisuales no estigmatizantes, que detonen el sentido de comunidad y refuercen su acervo cultural. El videojuego A Orillas del río es del género point and click, de vista isométrica, el cual está inspirado en el cuento oral tradicional El zorro y el coyote, que busca ser una herramienta lúdica de aprendizaje para reforzar el aprendizaje de la lengua Cucapá en los niños. Los resultados obtenidos fueron el demo del nivel uno (escenas uno y dos); colorimetría, arcos de personajes principales; programación de minijuegos. Dentro del documental ‘Déjennos pescar’ los resultados fueron un montaje, una clasificación del material grabado con transcripciones de audio, mientras que en la parte de estrategia de impacto se creó un manual de uso de redes sociales con colorimetría, tipografía, estilo de voz, tipo de contenido según la red social, para los futuros integrantes del equipo.ITESO, A.C

Mis casos clínicos de especialidades odontológicas

Libro que muestra la atención de casos clínicos particulares referente a las diferentes especialidades odontológicasLibro que muestra la atención de casos clínicos particulares referente a las diferentes especialidades odontológicasUniversidad Autónoma de Campeche Universidad Autónoma del Estado de Hidalgo Universidad Autónoma del Estado de Méxic

Estudio epidemiológico de caries dental en escolares del estado de Baja California, México, 2010 / 2010 Epidemiological Study of Dental Caries in Schoolchildren of the Baja California State, Mexico

Propósito: Determinar índices y prevalencia de caries dental en la población escolar del estado de Baja California, México, en el 2010. Métodos: Estudio de corte transversal realizado con los criterios y formato de encuesta de la Organización Mundial de la Salud (OMS), en tres grupos etarios (6, 12 y 15 años), en los cinco municipios del estado. Así mismo, se analizaron los resultados con relación al sexo y estrato socioeconómico. Los encuestadores fueron calibrados previamente (concordancia interexaminador kappa &gt; 0,80). La asociación del sexo y el nivel de ocupación de los padres sobre los indicadores de caries se evaluaron mediante χ2 para frecuencias y Andeva para efectos medios. El análisis se realizó con el paquete SAS 9,2. Resultados: La prevalencia de caries en dentición temporal a los 6 años fue del 60 %; en dentición permanente a los 12 años fue del 39 % y del 52 % a los 15. No hubo diferencias estadísticamente significativas respecto al sexo (p &gt; 0,05), pero sí respecto al tipo de ocupación de los padres. El índice ceo-d 6 años fue 2,58; el CPO-D 12 años, de 1,12, y de 1,88 a los 15. Conclusiones: La prevalencia de caries en dentición permanente pasó del 67,96 % en el 2001 al 39,53 % en el 2010. Según la OMS, el CPO-D de 1,12 a los 12 años sitúa a Baja California en índices muy bajos de caries; mientras que en el 2001 se hallaba en moderado, con CPO-D de 2,51. Hubo una mayor prevalencia de caries dental en los escolares con padres cuya ocupación requiere menor capacitación y estudio. Purpose: Determinate the prevalence and indexes of dental caries in the school population of the State of Baja California, Mexico, 2010. Methods: Cross-sectional study in schoolchildren carried out using the criteria and survey format provided by the World Health Organization (WHO), in three age groups (6, 12, and 15 years old), in the five municipalities of the state. Also, the results were analyzed in relation to sex and socioeconomic status of the school children. Interviewers were previously standardized (inter-examiner concordance kappa &gt; 0.80). The associations between sex and parent occupation and dental caries indicators were analyzed with the χ2 test for frequencies and Anova for media effects (SAS 9.2 statistical package). Results: The prevalence of dental caries in primary teeth at age 6 years old was 60 %; it was 39 % in permanent dentition at age 12 and 52 % among 15 years old. No statistically significant association was found for the variable sex (p&gt;0.05), but it was for the occupation of parents. The dmf-t index was 2.58 at 6 years of age, DMF-T 1.12 at 12 years, and 1.88 at age 15. Conclusions: The prevalence of dental caries in permanent teeth decreased from 67.96 % in 2001 to 39.53 % in 2010. According to the WHO, the DMF-T index 1.12 of 12 year-old schoolchildren from Baja California in 2010 is considered low, while it was moderate (DMF-T 2.51) in 2001. There was a higher prevalence of caries in schoolchildren with parents whose occupation requires lower education levels

Immunotolerance in liver transplantation: a primer for the clinician

The use of immunosuppressive medications for solid organ transplantation is associated with cardiovascular, metabolic, and oncologic complications. On the other hand, the development of graft rejection is associated with increased mortality and graft dysfunction. Liver transplant recipients can withdraw from immunosuppression without developing graft injury while preserving an adequate antimicrobial response - a characteristic known as immunotolerance. Immunotolerance can be spontaneously or pharmacologically achieved. Contrary to the classic dogma, clinical studies have elucidated low rates of true spontaneous immunotolerance (no serologic or histological markers of immune injury) among liver transplant recipients. However, clinical, serologic, and tissue biomarkers can aid in selecting patients in whom immunosuppression can be safely withdrawn. For those who failed an immunosuppression withdrawal trial or are at high risk of rejection, pharmacological interventions for immunotolerance induction are under development.In this review, we provide an overview of the mechanisms of immunotolerance, the clinical studies investigating predictors and biomarkers of spontaneous immunotolerance, as well as the potential pharmacological interventions for inducing it