FERMI-LAT Observations of Supernova Remnant G5.7-0.1, Believed to be Interacting with Molecular Clouds

This work reports on the detection of $\gamma$-ray emission coincident with the supernova remnant (SNR) SNR G5.7-0.1 using data collected by the Large Area Telescope aboard the Fermi Gamma-ray Space Telescope. The SNR is believed to be interacting with molecular clouds, based on 1720 MHz hydroxyl (OH) maser emission observations in its direction. This interaction is expected to provide targets for the production of $\gamma$-ray emission from $\pi^0$-decay. A $\gamma$-ray source was observed in the direction of SNR G5.7-0.1, positioned nearby the bright $\gamma$-ray source SNR W28. We model the emission from radio to $\gamma$-ray energies using a one-zone model. Following consideration of both $\pi^0$-decay and leptonically dominated emission scenarios for the MeV-TeV source, we conclude that a considerable component of the $\gamma$-ray emission must originate from the $\pi^0$-decay channel. Finally, constraints were placed on the reported ambiguity of the SNR distance through X-ray column density measurements made using XMM-Newton observations. We conclude SNR G5.7-0.1 is a significant $\gamma$-ray source positioned at a distance of $\sim 3$ kpc with luminosity in the 0.1--100 GeV range of $L_{\gamma} \approx 7.4 \times 10^{34}$ erg/s.Comment: 8 pages, 5 figures, 1 table, Accepted for publication in Ap

Intranasal Dexmedetomidine for Procedural Distress in Children: A Systematic Review.

CONTEXT: Intranasal dexmedetomidine (IND) is an emerging agent for procedural distress in children. OBJECTIVE: To explore the effectiveness of IND for procedural distress in children. DATA SOURCES: We performed electronic searches of Medline (1946-2019), Embase (1980-2019), Google Scholar (2019), Cumulative Index to Nursing and Allied Health Literature (1981-2019), and Cochrane Central Register. STUDY SELECTION: We included randomized trials of IND for procedures in children. DATA EXTRACTION: Methodologic quality of evidence was evaluated by using the Cochrane Collaboration\u27s risk of bias tool and the Grading of Recommendations Assessment, Development, and Evaluation system, respectively. The primary outcome was the proportion of participants with adequate sedation. RESULTS: Among 19 trials ( LIMITATIONS: The adequacy of sedation was subjective, which possibly led to biased outcome reporting. CONCLUSIONS: Given the methodologic limitations of included trials, IND is likely more effective at sedating children compared to oral chloral hydrate and oral midazolam. However, this must be weighed against the potential for adverse cardiovascular effects

PENGARUH LINGKUNGAN DAN STRESS KERJA TERHADAP KINERJA PEGAWAI PADA KANTOR KOMISI PEMILIHAN UMUM DI PROVINSI SULAWESI UTARA

ABSTRAK Penelitian ini bertujuan untuk menganalisa pengaruh lingkungan dan stress kerja terhadap kinerja pegawai pada Kantor Komisi Pemilihan Umum di Provinsi Sulawesi Utara. Populasi dalam penelitian ini adalah seluruh pegawai yang bekerja di Komisi Pemilihan Umum yang berada di Propinsi Sulawesi Utara berjumlah 242 pegawai. Sampel dalam penelitian ini adalah pegawai yang berada di 7 Kabupaten Kota yaitu Kab. Minahasa, Kab. Minahasa Selatan, Kab. Minahasa Utara, Kab. MInahasa Tenggaara, Kota Manado, Kota Bitung, dan Kota Tomohon. Jumlah sampel 140 pegawai, dimana 20 responden setiap kantor. Penelitian ini menggunakan stratified random sampling. Dari 140 kuisioner yang diedarkan, total kuisioner yang dapat digunakan dalam analisis berjumlah 74 kuisioner. Teknik analysis yang digunakan adalah regresi berganda dengan bantuan program spss 22. Hasil analisis mengidentifikasikan (1) lingkungan kerja berpengaruh signifikan terhadap kinerja pegawai, (2) stress kerja berpengaruh signifikan terhadap kinerja pegawai, (3) lingkungan kerja dan stress kerja secara bersama-sama berpengaruh terhadap kinerja pegawai. Kata- kata Kunci : Lingkungan kerja, Stress Kerja, Kinerja Pegawai, Komis Pemilihan Umum Provinsi Sulawesi Utara. ABSTRACT This study aims to analyze environmental influences and work stress on the performance of employees at the General Election Commission Office in North Sulawesi Province. The population in this study were all employees working in the General Election Commission in North Sulawesi Province totaling 242 employees. The sample in this study were employees who were in 7 City Regencies namely Kab. Minahasa, Kab. Minahasa Selatan, Kab. Minahasa Utara, Kab. Minahasa Tenggara, Kota Manado, Kota Bitung, and Kota Tomohon. The number of samples is 140 employees, of which 20 respondents are each office. This study uses stratified random sampling. Of the 140 questionnaires that were circulated, the total questionnaire that could be used in the analysis amounted to 74 questionnaires. The analysis technique used is multiple regression with the help of the SPSS 22 program. The results of the analysis identify (1) the work environment has a significant effect on employee performance, (2) work stress has a significant effect on employee performance, (3) the work environment and work stress together affect employee performance. Keywords : Work environment, Job Stress, Employee Performance, General Election Commission of North Sulawesi Province

Use of stable CHO pools and CHO TGE to accelerate SARS-CoV-2 vaccine development

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On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation

Although the genus Saccharomyces has been thoroughly studied, some species in the genus has not yet been accurately resolved; an example is S. bayanus, a taxon that includes genetically diverse lineages of pure and hybrid strains. This diversity makes the assignation and classification of strains belonging to this species unclear and controversial. They have been subdivided by some authors into two varieties (bayanus and uvarum), which have been raised to the species level by others. In this work, we evaluate the complexity of 46 different strains included in the S. bayanus taxon by means of PCR-RFLP analysis and by sequencing of 34 gene regions and one mitochondrial gene. Using the sequence data, and based on the S. bayanus var. bayanus reference strain NBRC 1948, a hypothetical pure S. bayanus was reconstructed for these genes that showed alleles with similarity values lower than 97% with the S. bayanus var. uvarum strain CBS 7001, and of 99¿100% with the non S. cerevisiae portion in S. pastorianus Weihenstephan 34/70 and with the new species S. eubayanus. Among the S. bayanus strains under study, different levels of homozygosity, hybridization and introgression were found; however, no pure S. bayanus var. bayanus strain was identified. These S. bayanus hybrids can be classified into two types: homozygous (type I) and heterozygous hybrids (type II), indicating that they have been originated by different hybridization processes. Therefore, a putative evolutionary scenario involving two different hybridization events between a S. bayanus var. uvarum and unknown European S. eubayanus-like strains can be postulated to explain the genomic diversity observed in our S. bayanus var. bayanus strains

Identification and Structural Characterization of a New Three-Finger Toxin Hemachatoxin from Hemachatus haemachatus Venom

10.1371/journal.pone.0048112PLoS ONE710

Potential of yeasts as biocontrol agents of the phytopathogen causing cacao Witches' Broom Disease: Is microbial warfare a solution?

Plant diseases caused by fungal pathogens are responsible for major crop losses worldwide, with a significant socio-economic impact on the life of millions of people who depend on agriculture-exclusive economy. This is the case of the Witches' Broom Disease (WBD) affecting cacao plant and fruit in South and Central America. The severity and extent of this disease is prospected to impact the growing global chocolate market in a few decades. WBD is caused by the basidiomycete fungus Moniliophthora perniciosa. The methods used to contain the fungus mainly rely on chemical fungicides, such as copper-based compounds or azoles. Not only are these highly ineffective, but also their utilization is increasingly restricted by the cacao industry, in part because it promotes fungal resistance, in part related to consumers' health concerns and environmental awareness. Therefore, the disease is being currently tentatively controlled through phytosanitary pruning, although the full removal of infected plant material is impossible and the fungus maintains persistent inoculum in the soil, or using an endophytic fungal parasite of Moniliophthora perniciosa which production is not sustainable. The growth of Moniliophthora perniciosa was reported as being antagonized in vitro by some yeasts, which suggests that they could be used as biological control agents, suppressing the fungus multiplication and containing its spread. Concurrently, some yeast-based products are used in the protection of fruits from postharvest fungal spoilage, and the extension of diverse food products shelf-life. These successful applications suggest that yeasts can be regarded a serious alternative also in the pre-harvest management of WBD and other fungal plant diseases. Yeasts' GRAS (Generally Recognized as Safe) nature adds to their appropriateness for field application, not raising major ecological concerns as do the present more aggressive approaches. Importantly, mitigating WBD, in a sustainable manner, would predictably have a high socioeconomic impact, contributing to diminish poverty in the cacao-producing rural communities severely affected by the disease. This review discusses the importance/advantages and the challenges that such a strategy would have for WBD containment, and presents the available information on the molecular and cellular mechanisms underlying fungi antagonism by yeasts.This work was supported by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI), and the project EcoAgriFood (NORTE-01-0145-FEDER-000009), supported by the Norte Portugal Regional Operational Programme (NORTE 2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF). PF is a Ph.D. student of the Doctoral Programme in Applied and Environmental Microbiology (DP_AEM) and FCT grantee PD/BD/113810/2015

Evaluation of genetic susceptibility to childhood allergy and asthma in an African American urban population

<p>Abstract</p> <p>Background</p> <p>Asthma and allergy represent complex phenotypes, which disproportionately burden ethnic minorities in the United States. Strong evidence for genomic factors predisposing subjects to asthma/allergy is available. However, methods to utilize this information to identify high risk groups are variable and replication of genetic associations in African Americans is warranted.</p> <p>Methods</p> <p>We evaluated 41 single nucleotide polymorphisms (SNP) and a deletion corresponding to 11 genes demonstrating association with asthma in the literature, for association with asthma, atopy, testing positive for food allergens, eosinophilia, and total serum IgE among 141 African American children living in Detroit, Michigan. Independent SNP and haplotype associations were investigated for association with each trait, and subsequently assessed in concert using a genetic risk score (GRS).</p> <p>Results</p> <p>Statistically significant associations with asthma were observed for SNPs in <it>GSTM1, MS4A2</it>, and <it>GSTP1 </it>genes, after correction for multiple testing. Chromosome 11 haplotype CTACGAGGCC (corresponding to <it>MS4A2 </it>rs574700, rs1441586, rs556917, rs502581, rs502419 and <it>GSTP1 </it>rs6591256, rs17593068, rs1695, rs1871042, rs947895) was associated with a nearly five-fold increase in the odds of asthma (Odds Ratio (OR) = 4.8, <it>p </it>= 0.007). The GRS was significantly associated with a higher odds of asthma (OR = 1.61, 95% Confidence Interval = 1.21, 2.13; <it>p </it>= 0.001).</p> <p>Conclusions</p> <p>Variation in genes associated with asthma in predominantly non-African ethnic groups contributed to increased odds of asthma in this African American study population. Evaluating all significant variants in concert helped to identify the highest risk subset of this group.</p

Two Chromogranin A-Derived Peptides Induce Calcium Entry in Human Neutrophils by Calmodulin-Regulated Calcium Independent Phospholipase A2

Background: Antimicrobial peptides derived from the natural processing of chromogranin A (CgA) are co-secreted with catecholamines upon stimulation of chromaffin cells. Since PMNs play a central role in innate immunity, we examine responses by PMNs following stimulation by two antimicrobial CgA-derived peptides. Methodology/Principal Findings: PMNs were treated with different concentrations of CgA-derived peptides in presence of several drugs. Calcium mobilization was observed by using flow cytometry and calcium imaging experiments. Immunocytochemistry and confocal microscopy have shown the intracellular localization of the peptides. The calmodulin-binding and iPLA2 activating properties of the peptides were shown by Surface Plasmon Resonance and iPLA2 activity assays. Finally, a proteomic analysis of the material released after PMNs treatment with CgA-derived peptides was performed by using HPLC and Nano-LC MS-MS. By using flow cytometry we first observed that after 15 s, in presence of extracellular calcium, Chromofungin (CHR) or Catestatin (CAT) induce a concentration-dependent transient increase of intracellular calcium. In contrast, in absence of extra cellular calcium the peptides are unable to induce calcium depletion from the stores after 10 minutes exposure. Treatment with 2-APB (2-aminoethoxydiphenyl borate), a store operated channels (SOCs) blocker, inhibits completely the calcium entry, as shown by calcium imaging. We also showed that they activate iPLA2 as the two CaM-binding factors (W7 and CMZ) and that the two sequences can be aligned with the two CaMbinding domains reported for iPLA2. We finally analyzed by HPLC and Nano-LC MS-MS the material released by PMNs following stimulation by CHR and CAT. We characterized several factors important for inflammation and innate immunity. Conclusions/Significance: For the first time, we demonstrate that CHR and CAT, penetrate into PMNs, inducing extracellular calcium entry by a CaM-regulated iPLA2 pathway. Our study highlights the role of two CgA-derived peptides in the active communication between neuroendocrine and immune systems

An English cover-up: masks, murders, and English cruelty in Goncourt, Lorrain, and Schwob

Fin-de-siècle writers from diverse disciplines were drawn to the seductive potential of masks and disguise; mask-wearing characters of indefinite identity, indeterminate gender, and insecure psychology proliferate in their texts. However, when characters are designated as English in such stories, they are also, and with remarkable frequency, associated with cruelty or murder: the mask-wielding murderers of Marcel Schwob’s ‘MM. Burke et Hare, Assassins’ carry out their crimes in Britain upon British victims; Edmond de Goncourt weaves his theatrical narrative around the mask-like demeanour of Lord Annandale in La Faustin; and Jean Lorrain’s malicious Lord Ethal exacerbates the Duc de Fréneuse’s perverse obsessions with masks in Monsieur de Phocas. This article explores this unexpected correlation, and examines the ways that English masks are used as narrative devices – at once to mould and play with national distinctions, and to reflect upon the psychological state of the French subject