Light Intensity Activity: a possible contribution to delay frailty

Trabalho apresentado em 24th Nordic Congress of Gerontology, 2-4 maio 2018, Oslo, NoruegaN/

Salt reduction in different bread types: an intervention study in the Northern Portugal

O pão é um dos alimentos mais antigos e consumidos em todo o Mundo, sendo considerado uma boa fonte de diversos nutrientes fundamentais para o desenvolvimento e saúde do organismo. Os dados do Inquérito Alimentar Nacional e de Atividade Física IAN-AF (2015/2016), em Portugal, identificam o pão como sendo considerado um dos principais alimentos a contribuir para a ingestão de sal dos portugueses. A Sociedade Portuguesa de Hipertensão, em colaboração com a Associação dos Industriais de Panificação, Pastelaria e Similares do Norte e o Instituto Nacional de Saúde Doutor Ricardo Jorge, desenvolveram um estudo interventivo com vista a reduzir o teor de sal no pão sem alterar as suas propriedades organoléticas. O objetivo deste estudo foi avaliar o teor de sal em 4 tipos de pão tradicional: carcaça, regueifa, broa e pão da avó, antes e depois da intervenção planeada. Antes da intervenção verificou-se que os valores de teor de sal encontrados, em todos os tipos de pão analisados, estavam abaixo de 1,4 g de sal por 100 g de pão (valor estabelecido pelo Decreto- -Lei n.º 75/2009, de 12 de agosto) e que, após intervenção, foi possível reduzir para valores entre 0,96 e 1,1 g de sal por 100 g de pão, sem alterar as características organoléticas. O estudo realizado demonstrou ser possível a redução do teor de sal no pão, sem alterações adicionais no processo de produção, constituindo uma medida de baixo custo que permite a melhoria da saúde pública, relativamente à prevenção de doenças crónicas associadas ao consumo excessivo de sal.Bread is a key staple in the Portuguese diet and provides many of the nutrients required for normal development and good health. The National Food and Physical Activity Survey IAN-AF (2015/2016), in Portugal, identify bread as one of the main foods contributing to Portuguese salt intake. The Portuguese Society of Hypertension, in collaboration with the Portuguese Bakeries Association from North and the National Institute of Health Dr. Ricardo Jorge, planned an intervention study to reduce the content of salt in the bread, without changing its organoleptic properties. The objective of this study was to evaluate the salt content in four types of traditional bread: “carcaça”, “regueifa”, “broa” e “pão da avó”, before and after the planned intervention. It was found that the values of salt content before the intervention were below the value established in legislation nº 75/2009 of 1.4 g of salt per 100 g of bread and that, after intervention it was possible to reduce the salt content to values between 0.96 and 1.1 g of salt per 100 g of bread, without changing the organoleptic characteristics. The study showed that it is possible to reduce the salt content in bread without additional changes in the production process, and it is a low-cost measure allowing the improvement of public health in relation to the prevention of chronic diseases associated with excessive salt consumptioninfo:eu-repo/semantics/publishedVersio

Elemental mapping of Portuguese ceramic pieces with a full-field XRF scanner based on a 2D-THCOBRA detector

UID/FIS/04559/2020In this work, we present a novel application of the full-field energy-dispersive X-ray fluorescence (EDXRF) imaging system based on a MicroPattern Gaseous Detector (2D-THCOBRA) in the cultural heritage field. The detector has an intrinsic imaging capability with spatial resolution of 400μmFWHM, and is energy sensitive, presenting an energy resolution of approximately 1keVFWHM at 5.9keV. The full-field XRF scanner based on the 2D-THCOBRA detector allows mapping the distribution of elements in large area samples with high detection efficiency (75 % at 5.9keV), being a very promising choice for elemental mapping analysis of large area cultural heritage samples. In this work, we have demonstrated the imaging capabilities of the full-field XRF scanner and used it to assess the restoration of a Portuguese faience piece.publishersversionpublishe

Meta-Analysis of MS-Based Proteomics Studies Indicates Interferon Regulatory Factor 4 and Nucleobindin1 as Potential Prognostic and Drug Resistance Biomarkers in Diffuse Large B Cell Lymphoma

Funding: Rune Matthiesen is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a Fiigureresult of the projects (iNOVA4Health— UIDB/04462/2020), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the project numbers: PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017.The prognosis of diffuse large B cell lymphoma (DLBCL) is inaccurately predicted using clinical features and immunohistochemistry (IHC) algorithms. Nomination of a panel of molecules as the target for therapy and predicting prognosis in DLBCL is challenging because of the divergences in the results of molecular studies. Mass spectrometry (MS)-based proteomics in the clinic represents an analytical tool with the potential to improve DLBCL diagnosis and prognosis. Previous proteomics studies using MS-based proteomics identified a wide range of proteins. To achieve a consensus, we reviewed MS-based proteomics studies and extracted the most consistently significantly dysregulated proteins. These proteins were then further explored by analyzing data from other omics fields. Among all significantly regulated proteins, interferon regulatory factor 4 (IRF4) was identified as a potential target by proteomics, genomics, and IHC. Moreover, annexinA5 (ANXA5) and nucleobindin1 (NUCB1) were two of the most up-regulated proteins identified in MS studies. Functional enrichment analysis identified the light zone reactions of the germinal center (LZ-GC) together with cytoskeleton locomotion functions as enriched based on consistent, significantly dysregulated proteins. In this study, we suggest IRF4 and NUCB1 proteins as potential biomarkers that deserve further investigation in the field of DLBCL sub-classification and prognosis.publishersversionpublishe

Dataset of the sperm proteome of stallions with different motility

This paper provides a detailed set of data on how the stal-lion sperm proteome differs among stallions with different sperm motilities, although within normal ranges. Findings distinguish proteins that may help to identify stallions of su-perior sperm motility. Sperm proteins were analyzed using a UHPLC/MS/MS system comprising of an Agilent 1290 in-finity series UHPLC coupled to an Agilent 6550 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). These data can be used to disclose potential targets to iden-tify good sperm samples and to study specific pathways in-volved in the regulation of sperm motility. This data article is linked to the paper "Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility Journal of Proteomics 247:104335 doi: 10.1016/j.jprot.2021.104335".(c) 2022 The Author(s). Published by Elsevier Inc.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/

Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy

Osteoporosis is a public health problem, with bone loss being the main consequence. Hydroxyapatite (HA) has been largely used as a bioceramic to stimulate bone growth. In our work, a cerium-containing HA (Ce-HA) has been proposed and its effects on the antimicrobial and bone-inducing properties were investigated. The synthesis of the materials occurred by the suspension–precipitation method (SPM). The XRD (X-ray Diffraction) confirmed the crystalline phase, and the Rietveld refinement confirmed the crystallization of HA and Ce-HA in a hexagonal crystal structure in agreement with ICSD n◦ 26205. Characterizations by FT-IR (Fourier Transform Infrared Spectroscopy), XPS (X-ray Photoemission Spectroscopy), and FESEM-EDS (Field Emission Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) confirmed the presence of cerium (Ce3+ and Ce4+ ). The antibacterial activity of Has was evaluated against Staphylococcus aureus 25,923 and Escherichia coli 25,922 strains, which revealed that the material has antimicrobial properties and the cytotoxicity assay indicated that Ce-containing HA was classified as non-toxic. The effects of Ce-HA on bone repair, after application in bone defects in the tibia of female rats with osteoporosis induced by ovariectomy (OVX), were evaluated. After 15 and 30 days of implantation, the samples were analyzed by Raman, histology and X-ray microtomography. The results showed that the animals that had the induced bone defects filled with the Ce-HA materials had more expressive bone neoformation than the control group.info:eu-repo/semantics/publishedVersio

Triatoma sordida: considerações sobre o tempo de vida das formas adultas e sobre a oviposição das fêmeas

Fifty three specimen of T. sordida which arrived at the adult stage were studied; there were 22 males and 31 females, all obtained from 110 eggs. The average time duration of life in the nymph phase was not statistically different between sexes, being 174.6 and 170.6 days respectively for mate and female. The females had a larger mean time duration of life as adults (503 days) than the males (284 days). During their adult life, 86.42% of the time corresponded to oviposition, the greater intensity of egg-laying ocurring during the first half of the period. The mean number of eggs was 570.8 and the ratio egg/day varied from 0.68 to 1.97.Foram observados 53 exemplares do T. sordida que chegaram à fase alada, oriundos de 110 ovos; os machos eram em número de 22 e as fêmeas 31. Os tempos médios de duração da fase de ninfa não foram estatisticamente diferentes entre os sexos, sendo 174,6 e 170,6 dias respectivamente para machos e fêmeas. O tempo médio de vida na fase alada foi significativamente maior para as fêmeas, com 503 dias para estas e 284 para os machos. O período de oviposição correspondeu a 86,42% do tempo de fase alada das fêmeas, com a postura tendo maior intensidade na primeira metade desta fase. Cada fêmea pôs em média 570,8 ovos, variando a postura de 0,68 a 1,97 ovos por dia

Quality of life in Dutch patients with primary biliary cholangitis:Discrepancies between patients’ perspectives and objective disease parameters

Aim: This study aims to assess the health-related quality of life (HRQoL) in a Dutch population of patients with primary biliary cholangitis (PBC) in relation to the prognosis and need for second line-therapy, based on both objective disease parameters and patients’ perspectives. Methods: In this cross-sectional multicenter study, HRQoL was assessed by using the Dutch PBC-40 according to objective clinical parameters and patients’ perspectives on treatment and prognosis. Results: In total, 178/269 (66%) patients responded; mean age 61.2 (SD 9.9) years and 165 (92.7%) women. The PBC-40 domain scores did not differ according to the GLOBE score response (p &gt; 0.05 for all) or according to the POISE criteria (p &gt; 0.05), except for the domain itch (p = 0.031). Patients who considered their survival to be impaired scored higher on all domains as compared to those expecting a normal prognosis (p &lt; 0.05). Similarly, PBC-40 domain scores were higher among patients who considered that they were in need of additional therapy compared to those who did not (p &lt; 0.05 for all, except for domain itch [p = 0.056]). However, 45/62 (72.6%) patients with a self-expected impaired prognosis had a GLOBE score indicative of a normal prognosis. Twenty-five of the 40 (62.5%) patients who believed they needed additional therapy were below POISE criteria. Conclusion: The HRQoL of patients with PBC was impaired in terms of nonfavorable disease status according to the expectations of patients, but not according to objective disease parameters. Substantial discrepancies between patients’ perspectives and objective parameters were observed, which highlights the need for better patient guidance among patient with PBC.</p