Early permanent dental eruption in obese/overweigh schoolchildren

The WHO defines obesity as abnormal excessive fat accumulation. Indeed, it is the most relevant nutritional disorder in the developed countries. Nutrition turns out to be relevant for childhood development involving different systems and organs as and in

A Distributed Control Strategy for Islanded Single-Phase Microgrids with Hybrid Energy Storage Systems Based on Power Line Signaling

Energy management control is essential to microgrids (MGs), especially to single-phase ones. To handle the variety of distributed generators (DGs) that can be found in a MG, e.g., renewable energy sources (RESs) and energy storage systems (ESSs), a coordinated power regulation is required. The latter are generally battery-based systems whose lifetime is directly related to charge/discharge processes, whereas the most common RESs in a MG are photovoltaic (PV) units. Hybrid energy storage systems (HESS) extend batteries life expectancy, thanks to the effect of supercapacitors, but they also require more complex control strategies. Conventional droop methodologies are usually applied to provide autonomous and coordinated power control. This paper proposes a method for coordination of a single-phase MG composed by a number of sources (HESS, RES, etc.) using power line signaling (PLS). In this distributed control strategy, a signal whose frequency is higher than the grid is broadcasted to communicate with all DGs when the state of charge (SoC) of the batteries reaches a maximum value. This technique prevents batteries from overcharging and maximizes the power contribution of the RESs to the MG. Moreover, different commands apart from the SoC can be broadcasted, just by changing to other frequency bands. The HESS master unit operates as a grid-forming unit, whereas RESs act as grid followers. Supercapacitors in the HESS compensate for energy peaks, while batteries respond smoothly to changes in the load, also expanding its lifetime due to less aggressive power references. In this paper, a control structure that allows the implementation of this strategy in single-phase MGs is presented, with the analysis of the optimal range of PLS frequencies and the required self-adaptive proportional-resonant controllers

Effects of mechanical box pruning intensity on bud development, vegetative growth, and yield components on cv. Cabernet-Sauvignon in Mendoza, Argentina

Mechanised winter pruning is increasingly being used to reduce the high costs of manual pruning. Mechanised pruning is non-selective, and for its optimal application, the definition of pruning intensity is necessary to achieve the target yield and grape quality. Our objectives were to evaluate the effects of three intensities of mechanical box pruning compared to a manual pruning treatment on spur length, the number of retained buds, budburst, shoot growth, and development; yield components and berry composition, and vine balance. Treatments corresponded to three mechanical pruning intensities leading to different bud loads at the beginning of the experiment by pruning at different distances from the cordon (i.e., box size): MP1 (1 cm height × 7 cm width), MP2 (14 cm × 14 cm), and MP3 (21 cm × 21 cm). MP2 treatment was also compared to a traditional manual pruning treatment (SP) that was pruned to the same bud/plant in the first season. In the first season, MP2 retained the same bud/plant as SP, but in the second and third seasons, MP2 retained 88 % and 100 % more buds/plant than SP, respectively. Accordingly, MP2 and SP showed no difference in yields in the first season, but in the second season, MP2 showed a 39 % higher yield than SP. Regarding the intensity of mechanical pruning, after treatments were applied, the number of retained buds increased as box size increased. Treatments modified the length of spurs, budburst percentage and their pattern. In the first season, the yield on the highest box (MP3) was four times higher than the smallest box (MP1), but they showed similar yields in the second season. The intensity of mechanical box pruning, applied after two seasons, generated regulatory and compensation mechanisms (i.e., lower budburst and cluster weight), leading to similar yields independently of the pruning intensity. Our results show that (i) after two seasons, mechanical pruning was more productive than manual pruning, and (ii) mechanical pruning intensity had a marked influence on yield only during the first year of application, while some compensation mechanisms among yield components equilibrate yield afterwards

Naltrexone Reverses Ethanol-Induced Rat Hippocampal and Serum Oxidative Damage

Naltrexone, an antagonist of �-opioid receptors, is clinically used as adjuvant therapy of alcohol dishabituation. The aim of the present work was to test the effect of 1 mg/kg body weight of naltrexone to revert oxidative stress-related biochemical alterations, in the hippocampus and serum of chronic alcoholic adult rats. Malondialdehyde concentration was increased and glutathione peroxidase activity was decreased in hippocampus and serum of alcohol-treated rats. Naltrexone treatment restored these alterations. The in vitro antioxidant ability of Ntx could not justify these effects considering the doses used. Thus this apparent protective effect of Ntx can only be attributed to its pharmacological effects, as herein discussed.Ministerio de Educacion y Ciencia SAF2010-21317 Universidad Catolica de Valencia "San Vicente Martir" 2012-029-001 Plan Nacional sobre Drogas 2010/059 AI/ICB-Santander 07/1

Oxidative stress-induced angiogenesis is mediated by miR-205-5p

miR-205-5p is known to be involved in VEGF-related angiogenesis and seems to reg-ulate associated cell signalling pathways, such as cell migration, proliferation and ap-optosis. Therefore, several studies have focused on the potential role of miR-205-5p as an anti-angiogenic factor. Vascular proliferation is observed in diabetic retinopathy and the ‘wet’ form of age-related macular degeneration. Today, the most common treatments against these eye-related diseases are anti-VEGF therapies. In addition, both AMD and DR are typically associated with oxidative stress; hence, the use of antioxidant agents is accepted as a co-adjuvant therapy for these patients. According to previous data, ARPE-19 cells release pro-angiogenic factors when exposed to oxi-dative insult, leading to angiogenesis. Matching these data, results reported here, indicate that miR-205-5p is modulated by oxidative stress and regulates VEGFA-angiogenesis. Hence, miR-205-5p is proposed as a candidate against eye-related pro-liferative diseasesUniversidad Católica de Valencia San Vicente Mártir research Fund and Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia San Vicente Mártir, Grant/Award Number: 2018-128-001, 2019-128-001; Escuela de Doctorado Universidad Católica de Valencia San Vicente Mártir, Grant/Award Number: EDUCV-PRE-2015-006 and EDUCV-PRE-2016-005Medicin

La evaluación por competencias en la Formación Profesional.: Una revisión sistemática de la producción científica española

Las reformas legislativas de la Formación Profesional han forzado un cambio de paradigma y han favorecido un enfoque competencial con consecuencias directas en la evaluación. En este trabajo de revisión sistemática, se han analizado las publicaciones relativas a la evaluación en la FP con clasificación superior a D en CIRC durante el periodo 2011-2021. Los resultados principales indican una baja producción con un periodo álgido en 2017-2018, con tendencia a la publicación puntual en parejas, pero con un impacto moderado. La nueva realidad legislativa y los nuevos enfoques educativos invitan a una necesidad de impulsar la investigación en este campo.Observatorio de la Innovación Educativa2022-2

Differential hippocampal response to chronic alcohol consumption of young adult and mature adult rats

AIMS Early ethanol consumption could be a risk factor for young brain integrity and its maturation, and also for the development of addictive behaviors in adulthood. Neuronal nitric oxide synthase (nNOS) expressing neurons are specifically located in the subgranular layer (SGL) of dentate gyrus and may be relevant for hippocampal neurogenesis. The focus of this work is aimed to determine local changes in the nNOS-like immunoreactive (nNOS-LIR) cell populations of the SGL after chronic ethanol exposure in young adult and mature adult rats. METHODS We used the nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase (NADPH-d) reaction as a qualitative marker of nNOS enzyme activity. We also analyzed the nNOS-LIR cell density by the nNOS immunocytochemistry in order to compare these two methods of labeling. Dorsal striatum (CPu) was also analyzed in order to compare two neural areas with high nNOS-LIR cell density. RESULTS The young adult group showed less hippocampal NADPH-d(+) cell density than the mature adult group. Interestingly, the NADPH-d(+) cell density was increased in the SGL of the young adult ethanol-treated group, whereas it decreased in the mature adult ethanol-treated group, when compared with their respective controls. No change was observed in any of the groups for the hippocampal nNOS-LIR cell density and no differences could be established in CPu for nNOS-LIR and NADPH-d(+) cell densities in any of the groups studied. CONCLUSION The NADPH-d expression is affected by chronic ethanol exposure in opposite ways between both age groups studied. Further studies are needed to evaluate the relative importance of these findings, especially when considering human subject

Role of hippocampal NF-κB and GluN2B in the memory acquisition impairment of experiences gathered prior to cocaine administration in rats

Cocaine can induce severe neurobehavioral changes, among others, the ones involved in learning and memory processes. It is known that during drug consumption, cocaine-associated memory and learning processes take place. However, much less is known about the effects of this drug upon the mechanisms involved in forgetting.The present report focuses on the mechanisms by which cocaine affects memory consolidation of experiences acquired prior to drug administration. We also study the involvement of hippocampus in these processes, with special interest on the role of Nuclear factor kappa B (NF-κB), N-methyl-D-aspartate glutamate receptor 2B (GluN2B), and their relationship with other proteins, such as cyclic AMP response element binding protein (CREB). For this purpose, we developed a rat experimental model of chronic cocaine administration in which spatial memory and the expression or activity of several proteins in the hippocampus were assessed after 36 days of drug administration. We report an impairment in memory acquisition of experiences gathered prior to cocaine administration, associated to an increase in GluN2B expression in the hippocampus. We also demonstrate a decrease in NF-κB activity, as well as in the expression of the active form of CREB, confirming the role of these transcription factors in the cocaine-induced memory impairment

Plasmid flux in Escherichia coli ST131 sublineages, analyzed by plasmid constellation network (PLACNET), a new method for plasmid reconstruction from whole genome sequences

This is an open-access article distributed under the terms of the Creative Commons Attribution License.Bacterial whole genome sequence (WGS) methods are rapidly overtaking classical sequence analysis. Many bacterial sequencing projects focus on mobilome changes, since macroevolutionary events, such as the acquisition or loss of mobile genetic elements, mainly plasmids, play essential roles in adaptive evolution. Existing WGS analysis protocols do not assort contigs between plasmids and the main chromosome, thus hampering full analysis of plasmid sequences. We developed a method (called plasmid constellation networks or PLACNET) that identifies, visualizes and analyzes plasmids in WGS projects by creating a network of contig interactions, thus allowing comprehensive plasmid analysis within WGS datasets. The workflow of the method is based on three types of data: assembly information (including scaffold links and coverage), comparison to reference sequences and plasmid-diagnostic sequence features. The resulting network is pruned by expert analysis, to eliminate confounding data, and implemented in a Cytoscape-based graphic representation. To demonstrate PLACNET sensitivity and efficacy, the plasmidome of the Escherichia coli lineage ST131 was analyzed. ST131 is a globally spread clonal group of extraintestinal pathogenic E. coli (ExPEC), comprising different sublineages with ability to acquire and spread antibiotic resistance and virulence genes via plasmids. Results show that plasmids flux in the evolution of this lineage, which is wide open for plasmid exchange. MOBF12/IncF plasmids were pervasive, adding just by themselves more than 350 protein families to the ST131 pangenome. Nearly 50% of the most frequent γ–proteobacterial plasmid groups were found to be present in our limited sample of ten analyzed ST131 genomes, which represent the main ST131 sublineages.Work was financed by the Spanish Ministry of Economy and Competitivity (BFU2011-26608 to FdlC, FIS-PI09/01273 and AGL2013-47852-R to JB and FIS-PI12-01581 and CB06/02/0053 to TMC), by the European Seventh Framework Program (612146/FP7-ICT-2013-10 to FdlC and 282004/FP7-HEALTH-2011-2.3.1-2 to FdlC and TMC); by Red Española de Investigación en Patología­ Infecciosa (REIPI RD06/0008/1018-1016) to JB, by Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia and European Regional Development Fund, ERDF (CN2012/303 and EM2014/001) to JB and by the regional government of Madrid (PROMPT-S2010/BMD2414) to TMC. We are also grateful to the Spanish Network for the Study of Plasmids and Extrachromosomal Elements (REDEEX) for funding cooperation among Spanish microbiologists working on the biology of MGEs (Spanish Ministry of Science and Innovation BFU2011-14145-E).Peer Reviewe