Finding qualitative research: an evaluation of search strategies

BACKGROUND: Qualitative research makes an important contribution to our understanding of health and healthcare. However, qualitative evidence can be difficult to search for and identify, and the effectiveness of different types of search strategies is unknown. METHODS: Three search strategies for qualitative research in the example area of support for breast-feeding were evaluated using six electronic bibliographic databases. The strategies were based on using thesaurus terms, free-text terms and broad-based terms. These strategies were combined with recognised search terms for support for breast-feeding previously used in a Cochrane review. For each strategy, we evaluated the recall (potentially relevant records found) and precision (actually relevant records found). RESULTS: A total yield of 7420 potentially relevant records was retrieved by the three strategies combined. Of these, 262 were judged relevant. Using one strategy alone would miss relevant records. The broad-based strategy had the highest recall and the thesaurus strategy the highest precision. Precision was generally poor: 96% of records initially identified as potentially relevant were deemed irrelevant. Searching for qualitative research involves trade-offs between recall and precision. CONCLUSIONS: These findings confirm that strategies that attempt to maximise the number of potentially relevant records found are likely to result in a large number of false positives. The findings also suggest that a range of search terms is required to optimise searching for qualitative evidence. This underlines the problems of current methods for indexing qualitative research in bibliographic databases and indicates where improvements need to be made

Sustainability, corporate community engagement and enterprise development: lessons from a case study in Emu-Ebendo Niger-Delta, Nigeria.

Corporate community engagement remains an under researched area in sustainability debates. Presenting a case study, based on in-depth interviews and site visits, of how one indigenous company Energia Ltd has formed a strong partnership or bond with the Emu-Ebendo community in the oil rich Niger Delta, the dynamics of corporate community engagement are critically discussed. Evidence of how both the company and community has benefited from this relationship is presented. The case analysis concludes that the company has effectively utilised a clearer understanding of the culture of the host community, leading to a transformational relationship. The approach adopted by Energia Ltd in the Emu-Ebendo community is unique in context. The key conclusion reached is that community benefit funds and community enterprise development, as a derivative of corporate engagement, can play a transformational role in societal and economic development

Genetic Testing Reveals Germline Mutations Among Patients Undergoing Surgery for Colorectal Carcinoma in a Community Hospital Setting

Introduction: Defined germline mutations contribute to 5% to 10% of cases of colorectal carcinoma (CRC). While protocols for universal tumor screening have been adopted to detect mismatch repair (MMR) protein deficiency, widespread multigene panel testing has not been achieved. Barriers to implementing testing protocols may occur in community settings. Methods: A total of 160 patients presenting for surgical management of CRC between 2011 and 2020 were considered for retrospective analysis in a single-surgeon, single-institution, community-based cohort. The rate of multigene panel testing and prevalence of germline mutations were calculated, and patient characteristics were assessed. Results: A total of 32/160 (20%) patients underwent multigene panel testing, with 14/160 (9%) patients having germline mutations. While 88% of patients underwent panel testing after CRC diagnosis, 43% of these patients would have met testing criteria before diagnosis. Among the patients meeting criteria before diagnosis, 50% were found to carry a germline mutation. Discussion: The prevalence of germline mutations was similar to previously reported values, while the rate of multigene panel testing was higher than previously reported. These results may be unique to the study setting or result from multidisciplinary conference discussion. A significant number of patients with abnormal panel testing were not tested before CRC diagnosis, despite meeting the criteria. This finding represents a missed opportunity for risk stratification and underscores the importance of addressing testing barriers in the primary care setting. Conclusions: Primary care providers and oncologists in community hospitals must remain cognizant of changing guidelines as multigene panel testing becomes increasingly available

Covalent attachment of active enzymes to upconversion phosphors allows ratiometric detection of substrates

Upconverting phosphors (UCPs) convert multiple low energy photons into higher energy emission via the process of photon upconversion and offer an attractive alternative to organic fluorophores for use as luminescent probes. Here, UCPs were capped with functionalized silica in order to provide a surface to covalently conjugate proteins with surface?accessible cysteines. Variants of green fluorescent protein (GFP) and the flavoenzyme pentaerythritol tetranitrate reductase (PETNR) were then attached via maleimide?thiol coupling in order to allow energy transfer from the UCP to the GFP or flavin cofactor of PETNR, respectively. PETNR retains its activity when coupled to the UCPs, which allows reversible detection of enzyme substrates via ratiometric sensing of the enzyme redox state

How makeup makes other women jealous

First paragraph: Imagine you’re sitting at an important job interview. You’re suitably dressed and have taken the time to do your hair and apply some makeup. You’re smiling, answering questions, and attempting to appear competent, and, obviously, hireable.  Access article on The Conversation website at:https://theconversation.com/how-makeup-makes-other-women-jealous-60744

Positive Facial Affect Looks Healthy

A healthy appearance is linked to important behavioural outcomes. Here we investigated whether positive facial affect is a cue for perceived health. In study one, two groups of participants rated the perceived health or perceived happiness of a large set of faces with neutral expressions. Perceived happiness predicted perceived health, as did anthropometric measures of expression. In a second experimental study, we collected ratings of perceived health for a wide age range of target faces with either neutral or smiling expressions. Smiling faces were rated as being much healthier looking than neutral faces, confirming that facial expression plays a role in the perception of health. A third study investigating attractiveness as a possible mediator found that expression still had a significant direct effect on perceived health, after accounting for attractiveness. Together, these studies systematically show that facial affect plays a critical role in shaping our perceptions of health in others

Rfam: annotating non-coding RNAs in complete genomes

Rfam is a comprehensive collection of non-coding RNA (ncRNA) families, represented by multiple sequence alignments and profile stochastic context-free grammars. Rfam aims to facilitate the identification and classification of new members of known sequence families, and distributes annotation of ncRNAs in over 200 complete genome sequences. The data provide the first glimpses of conservation of multiple ncRNA families across a wide taxonomic range. A small number of large families are essential in all three kingdoms of life, with large numbers of smaller families specific to certain taxa. Recent improvements in the database are discussed, together with challenges for the future. Rfam is available on the Web at http://www.sanger.ac.uk/Software/Rfam/ and http://rfam.wustl.edu/