891 research outputs found
Evaluation of modelling approaches for predicting the spatial distribution of soil organic carbon stocks at the national scale
Soil organic carbon (SOC) plays a major role in the global carbon budget. It
can act as a source or a sink of atmospheric carbon, thereby possibly
influencing the course of climate change. Improving the tools that model the
spatial distributions of SOC stocks at national scales is a priority, both for
monitoring changes in SOC and as an input for global carbon cycles studies. In
this paper, we compare and evaluate two recent and promising modelling
approaches. First, we considered several increasingly complex boosted
regression trees (BRT), a convenient and efficient multiple regression model
from the statistical learning field. Further, we considered a robust
geostatistical approach coupled to the BRT models. Testing the different
approaches was performed on the dataset from the French Soil Monitoring
Network, with a consistent cross-validation procedure. We showed that when a
limited number of predictors were included in the BRT model, the standalone BRT
predictions were significantly improved by robust geostatistical modelling of
the residuals. However, when data for several SOC drivers were included, the
standalone BRT model predictions were not significantly improved by
geostatistical modelling. Therefore, in this latter situation, the BRT
predictions might be considered adequate without the need for geostatistical
modelling, provided that i) care is exercised in model fitting and validating,
and ii) the dataset does not allow for modelling of local spatial
autocorrelations, as is the case for many national systematic sampling schemes
Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro
Mycoplasma hyopneumoniae, the agent of porcine enzootic pneumonia (EP), is able to persist in the lung tissue and evade destruction by the host for several weeks. To understand the mechanism of pathogen survival, phagocytic uptake of M. hyopneumoniae by primary porcine alveolar macrophages was investigated. Intracellular location and survival of the pathogen were explored using gentamicin survival assays, flow cytometry and confocal microscopy of M. hyopneumoniae 232 labelled with green fluorescent protein (GFP). Following 1 h and 16 h of co-incubation, few viable M. hyopneumoniae were recovered from inside macrophages. Flow cytometric analysis of macrophages incubated with M. hyopneumoniae expressing GFP indicated that the mycoplasmas became associated with macrophages, but were shown to be extracellular when actin-dependent phagocytosis was blocked with cytochalasin D. Confocal microscopy detected GFP-labelled M. hyopneumoniae inside macrophages and the numbers increased modestly with time of incubation. Neither the addition of porcine serum complement or convalescent serum from EP-recovered pigs was able to enhance engulfment of M. hyopneumoniae. This investigation suggests that M. hyopneumoniae evades significant uptake by porcine alveolar macrophages and this may be a mechanism of immune escape by M. hyopneumoniae in the porcine respiratory tract
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Basis for a Waste Management Public Communication Policy: Actual Situation Analysis and Implementation of Corrective Actions
Argentina will require new sites for the location of radioactive waste final disposal systems. It is currently mandatory to have social and political consensus to obtain the corresponding agreements. The experience obtained with the cancellation of the project ''Feasibility Study and Engineering Project--Repository for High Level Radioactive Waste'', reinforces even more the necessity to count with the acceptance of the public to carry out projects of this kind. The first phase of the former was developed in the 80's: geological, geophysical and hydrogeological studies were performed in a compact granitic rock located in Sierra del Medio, Chubut province. This project had to be called off in the early 90's due to strong social rejection. This decision was closely related to the poor attention given to social communication issues. The governmental decision-makers in charge underwent a lot of pressure from social groups claiming for the cancellation of the project due to the lack of information and the fear it triggered. Thus, the lesson learnt: ''social communication activities must be carefully undertaken in order to achieve the appropriate management of the radioactive waste produced in our country.'' The same as in other countries, the specific National Law demands the formulation of a Strategic Plan which will not only include the research into radioactive waste, but the design of a Social Communication Programme as well. The latter will be in charge of informing the population clearly and objectively about the latest scientific and technological advances in the issue. A tentative perception-attitude pattern of the Argentine society about the overall nuclear issue is outlined in this paper. It is meant to contribute to the understanding of the public's adverse reaction to this kind of project. A communication programme is also presented. Its objective is to install the waste management topic in the public's opinion with a positive real outlook
Large magnetic anisotropy in Ferrihydrite nanoparticles synthesized from reverse micelles
Six-line ferrihydrite(FH) nanoparticles have been synthesized in the core of
reverse micelles, used as nanoreactors to obtain average particle sizes
2 to 4 nm. The blocking temperatures extracted from
magnetization data increased from to 20 K for increasing particle
size. Low-temperature \MOS measurements allowed to observe the onset of
differentiated contributions from particle core and surface as the particle
size increases. The magnetic properties measured in the liquid state of the
original emulsion showed that the \FH phase is not present in the liquid
precursor, but precipitates in the micelle cores after the free water is
freeze-dried. Systematic susceptibility \chi_{ac}(\emph{f},T) measurements
showed the dependence of the effective magnetic anisotropy energies
with particle volume, and yielded an effective anisotropy value of kJ/m.Comment: 8 pages, 10 figures. Nanotechnology, v17 (Nov. 2006) In pres
Use of automated change detection and VGI sources for identifying and validating urban land use change
© 2020, by the authors. Land use and land cover (LULC) mapping is often undertaken by national mapping agencies, where these LULC products are used for different types of monitoring and reporting applications. Updating of LULC databases is often done on a multi-year cycle due to the high costs involved, so changes are only detected when mapping exercises are repeated. Consequently, the information on LULC can quickly become outdated and hence may be incorrect in some areas. In the current era of big data and Earth observation, change detection algorithms can be used to identify changes in urban areas, which can then be used to automatically update LULC databases on a more continuous basis. However, the change detection algorithm must be validated before the changes can be committed to authoritative databases such as those produced by national mapping agencies. This paper outlines a change detection algorithm for identifying construction sites, which represent ongoing changes in LU, developed in the framework of the LandSense project. We then use volunteered geographic information (VGI) captured through the use of mapathons from a range of different groups of contributors to validate these changes. In total, 105 contributors were involved in the mapathons, producing a total of 2778 observations. The 105 contributors were grouped according to six different user-profiles and were analyzed to understand the impact of the experience of the users on the accuracy assessment. Overall, the results show that the change detection algorithm is able to identify changes in residential land use to an adequate level of accuracy (85%) but changes in infrastructure and industrial sites had lower accuracies (57% and 75 %, respectively), requiring further improvements. In terms of user profiles, the experts in LULC from local authorities, researchers in LULC at the French national mapping agency (IGN), and first-year students with a basic knowledge of geographic information systems had the highest overall accuracies (86.2%, 93.2%, and 85.2%, respectively). Differences in how the users approach the task also emerged, e.g., local authorities used knowledge and context to try to identify types of change while those with no knowledge of LULC (i.e., normal citizens) were quicker to choose 'Unknown' when the visual interpretation of a class was more difficult
Elucidating the crystal-chemistry of Jbel Rhassoul stevensite (Morocco) by advanced analytical techniques
The composition of Rhassoul clay is controversial regarding the nature of the puremineral clay fraction which is claimed to be stevensite rather than saponite. In this study, the raw and mineral fractions were characterized using various techniques including Fourier transform infrared spectroscopy and magic angle spinning nuclear magnetic resonance (MAS NMR). The isolated fine clay mineral fraction contained a larger amount of Al (>1 wt.%) than that reported for other stevensite occurrences. The 27Al MAS NMR technique confirmed that the mineral is stevensite in which the Al is equally split between the tetrahedral and octahedral coordination sites. The 29Si NMR spectrum showed a single unresolved resonance indicating little or no short-range ordering of silicon. The chemical composition of the stevensite from Jbel Rhassoul was determined to be ((Na0.25K0.20 (Mg5.04Al0.37Fe0.20&0.21)5.61(Si7.76Al0.24)8O20(OH)4). This formula differs from previous compositions described from this locality and shows it to be an Al-bearing lacustrine clay mineral
Segmented Hellenic slab rollback driving Aegean deformation and seismicity
The NE dipping slab of the Hellenic subduction is imaged in unprecedented detail using
teleseismic receiver function analysis on a dense 2-D seismic array. Mapping of slab geometry for over 300 km along strike and down to 100 km depth reveals a segmentation into dipping panels by along-dip faults. Resolved intermediate-depth seismicity commonly attributed to dehydration embrittlement is shown to be clustered along these faults. Large earthquakes occurrence within the upper and lower plate and at the interplate megathrust boundary show a striking correlation with the slab faults suggesting high mechanical coupling between the two plates. Our results imply that the general slab rollback occurs here in a differential piecewise manner imposing its specific stress and deformation pattern onto the overriding Aegean plate
Beliefs on the local effects of climate change: causal attribution of flooding and shoreline retreat
Adaptation to climate change is a process that should engage different participants, including not only researchers and technicians but also other stakeholders and local individuals, and, therefore, it is important to understand their beliefs on the local effects of climate change. Recent studies illustrate a linear relation between coastal distance and scepticism, which is lower in coastal zones than in inland. A possible explanation is that people living inland do not experience (or do not perceive) particular natural hazards as being caused by climate change, or attribute the natural hazards to other causes, apart from climate change. This might influence the relative importance of dealing with direct anthropogenic effects and planning adaptation to climate change. Therefore, the goal of this work was to explore this effect by comparing beliefs on the local effects of climate change in Aveiro region (Portugal), specifically in Baixo Vouga Lagunar (BVL, located in the inner side of Ria de Aveiro Coastal Lagoon, 10 km distance from the coast) with the nearby coastal zone between Esmoriz and Vagueira settlements. Stakeholders were interviewed and local individuals were surveyed in order to explore causal attributions towards relevant local environmental problems and compare with data available from the coastal zone. Natural hazards concerned flooding in BVL and shoreline retreat in the coastal zone. Results suggest that in BVL both stakeholders and local residents did not attribute local natural hazards mostly to climate change. However, in the coastal zone, local natural hazards were indeed mostly attributed to climate change. This attribution to climate change was further correlated to a higher risk perception of natural hazards in the coastal zone but not in BVL. Thereby, it is important to consider distance from the shoreline in order to promote local processes of adaptation to climate change.info:eu-repo/semantics/publishedVersio
A severe case of pneumopathy in a duck breeder due to Chlamydia psittaci diagnosed by 16S rDNA sequencing
Introduction: Psittacosis is a zoonotic infectious disease contracted from birds and caused by Chlamydia psittaci, an obligate intracellular pathogen. In humans, the symptoms of the disease range from inapparent illness to systemic illness with severe pneumonia.
Case presentation: A severe case of atypical pneumonia requiring extra‐corporeal membrane oxygenation in a duck breeder is described. Because of the critical urgency of the case described here, and without any clear identification of the pathogen during the first days of hospitalization, treatment had to be adjusted daily. While conventional clinical methods failed to identify the causative agent, C. psittaci was finally identified using broad‐range 16S rDNA PCR analysis performed on a sample of broncho‐alveolar fluid.
Conclusion: Owing to the non‐specific clinical signs of psittacosis, early identification of cases of the disease remains a challenge. C. psittaci should be sought in patients presenting severe acute respiratory distress syndrome without any evidence of other infectious causes and especially when exposure to birds or bird products is reported. PCR is a very useful method to help identify fastidious organisms of this kind
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