Aktivitas Uji Antimitotik Senyawa Asam Heksadekanoat Isolat Dari Hydroid Aglaophenia Cupressina Lamoureoux Pada Cleavage Bulu Babi Tripneustes Gratilla Linn

Studi tentang aktivitas antimitotik senyawa asam heksadekanoat isolat dari hydroid Aglaopehenia cupressina Lamoureoux pada cleavage bulu babi Tripneustes gratilla Linn. Penelitian ini bertujuan untuk mengetahui pengaruh senyawa bioaktif asam heksadekanoat dari hydroid Aglaopehenia cupressina Lamoureoux pada cleavage bulu babi Tripneustes gratilla Linn. Metode yang digunakan adalah metode uji aktivitas antimitotik senyawa asam heksadekanoat pada cleavage bulu babi Tripneustes gratilla Linn. pada konsentrasi 1, 10, dan 100 µg/ml dengan kontrol positif vinkristin pada konsentrasi 0.01, 0.1, dan 1 µg/ml setelah fertilisasi penghambatan tertinggi terjadi pada konsentrasi 100 µg/ml yaitu 66,66 %. Kemudian setelah dihitung dengan analisis probit, asam heksadekanoat memiliki IC50 sebesar 22,076 µg/ml yang memiliki kesamaan sifat antimitotik dengan vinkristin yang memiliki IC 50 sebesar 0,219 µg/ml. Sehingga asam heksadekanoat isolat dari hydroid Aglaopehenia cupressina Lamoureoux berpotensi untuk dijadikan bahan dasar anti kanke

Uji Toksisitas Ekstrak Daun Jeruju Acanthus Ilicifolius Terhadap Artemia Salina Leach.

Jeruju Acanthus ilicifolius adalah tumbuhan mangrove yang memiliki berbagai potensi dalam bidang pangan dan kesehatan. Untuk dikembangkan sebagai tanaman obat perlu dilakukan penelitianlebih lanjut tentang bioaktivitas ekstrak daun jeruju terhadap A. Salina sebagai uji awal. Penelitian ini bertujuan untuk mengetahui toksisitas ekstrak daun A.illicifolius terhadap artemia salina L. Pengambilan sampel di Karst Ramang Ramang Kabupaten Maros Provinsi Sulawesi Selatan. Uji toksisitas dilakukan di lakukan di laboratorium Biokimia Departemen Kimia Fakultas MIPA Unhas, dengan menggunakan metode Brine Shrimp Lethality Test (BSLT). Hasil yang diperoleh ekstrak daun jeruju A. ilicifolius memiliki bioaktivitas tinggi dengan nilai LC50 = 7,56µg/mL. Diharapkan jeruju A. Ilicifolius dapat dikembangkan sebagai tanaman obat yang memiliki potensi dalam mengobati penyakit

Isolasi, Karakterisasi, dan Uji Bioaktivitas Metabolit Sekunder dari Hydroid Aglaophenia Cupressina Lamoureoux sebagai Bahan Dasar Antimikroba

Isolation, structure elucidation, activity test against Salmonella thypi, Staphylococcus aureus of secondary metabolite from hydroid Aglaophenia cupressina Lamoureoux, were conducted. This study was aimed to identify and isolate the compound with antibacterial properties of hydroid Aglaophenia crupessina Lamoureoux. This study used stepwise method with the following sequences: extraction, isolation, purification, structure elucidation, and bioactivity test. Study results indicated three compounds found: (1)carboxylate acid compound: hexadecanoic acid and has antibacterial properties; (2) alkaloidcompound which was considered as a new compound: aglaounhas and has antimicrobialproperties; (3) steroid compound: β-sitosterol with no antimicrobial properties

Bioaktivitas Ekstrak Daun Avicenia Marina terhadap Pertumbuhan Bakteri Staphylococcus Aureus

Avicenia marina yang dikenal dengan nama api-api, merupakan salah satu spesies mangrove, memiliki manfaat dalam bidang pangan dan kesehatan, yang berpotensi untuk dikembangkan. Penelitian ini bertujuan mengetahui aktivitas senyawa bioaktif yang terdapat pada ekstrak daun A. marina dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Sampel A. marina diperoleh dari Karst Ramang-Ramang Kabupaten Maros Propinsi Sulawesi Selatan. Uji antibakteri dilakukan di laboratorium mikrobiologi Departemen Biologi fakultas MIPA Unhas. Hasil uji antibakteri menunjukkan A. Marina, bersifat bakteriostatik terhadap bakteri Staphylococcus aureus

Qualitative and quantitative characterization of exosomes secreted by rat hepatocytes

Comunicaciones a congreso

PHANGS CO kinematics: disk orientations and rotation curves at 150 pc resolution

We present kinematic orientations and high resolution (150 pc) rotation curves for 67 main sequence star-forming galaxies surveyed in CO (2-1) emission by PHANGS-ALMA. Our measurements are based on the application of a new fitting method tailored to CO velocity fields. Our approach identifies an optimal global orientation as a way to reduce the impact of non-axisymmetric (bar and spiral) features and the uneven spatial sampling characteristic of CO emission in the inner regions of nearby galaxies. The method performs especially well when applied to the large number of independent lines-of-sight contained in the PHANGS CO velocity fields mapped at 1'' resolution. The high resolution rotation curves fitted to these data are sensitive probes of mass distribution in the inner regions of these galaxies. We use the inner slope as well as the amplitude of our fitted rotation curves to demonstrate that CO is a reliable global dynamical mass tracer. From the consistency between photometric orientations from the literature and kinematic orientations determined with our method, we infer that the shapes of stellar disks in the mass range of log($\rm M_{\star}(M_{\odot})$)=9.0-10.9 probed by our sample are very close to circular and have uniform thickness.Comment: 19 figures, 36 pages, accepted for publication in ApJ. Table of PHANGS rotation curves available from http://phangs.org/dat

Whole exome sequencing enhanced imputation identifies 85 metabolite associations in the Alpine CHRIS cohort

Metabolites are intermediates or end products of biochemical processes involved in both health and disease. Here, we take advantage of the well-characterized Cooperative Health Research in South Tyrol (CHRIS) study to perform an exome-wide association study (ExWAS) on absolute concentrations of 175 metabolites in 3294 individuals. To increase power, we imputed the identified variants into an additional 2211 genotyped individuals of CHRIS. In the resulting dataset of 5505 individuals, we identified 85 single-variant genetic associations, of which 39 have not been reported previously. Fifteen associations emerged at ten variants with \u3e5-fold enrichment in CHRIS compared to non-Finnish Europeans reported in the gnomAD database. For example, the CHRIS-enriche

Pathology of Echinococcosis

Infection of humans by the larval stage of the tapeworms Echinococcus granulosus sensu lato or Echinococcus multilocularis causes the life-threatening zoonoses cystic echinococcosis (CE) and alveolar echinococcosis (AE). Although cystic liver lesions are a hallmark of both diseases, course, prognosis, and patients' management decisively differ between the two. The wide and overlapping spectrum of morphologies and the limited availability of ancillary tools are challenges for pathologists to reliably diagnose and subtype echinococcosis. Here, we systematically and quantitatively recorded the pathologic spectrum in a clinically and molecularly defined echinococcosis cohort (138 specimens from 112 patients). Immunohistochemistry using a novel monoclonal antibody (mAbEmG3) was implemented, including its combined application with the mAbEm2G11. Six morphologic criteria sufficiently discriminated between CE and AE: size of smallest (CE/AE: >2/≤2 mm) and largest cyst (CE/AE: >25/≤25 mm), thickness of laminated layer (CE/AE: >0.15/≤0.15 mm) and pericystic fibrosis (CE/AE: >0.6/≤0.6 mm), striation of laminated layer (CE/AE: moderate-strong/weak), and number of cysts (CE/AE: ≤9/>9). Combined immunohistochemistry with mAbEm2G11 (E. multilocularis specific) and mAbEmG3 (reactive in AE and CE) was equally specific as and occasionally more sensitive than polymerase chain reaction. On the basis of these findings, we developed a diagnostic algorithm for the differential diagnosis of echinococcosis. In summary, we have not only identified the means to diagnose echinococcosis with greater certainty, but also defined morphologic criteria, which robustly discriminate between CE and AE. We expect our findings to improve echinococcosis diagnostics, especially of challenging cases, beneficially impacting the management of echinococcosis patients

Pathology of Echinococcosis: A Morphologic and Immunohistochemical Study on 138 Specimens With Focus on the Differential Diagnosis Between Cystic and Alveolar Echinococcosis.

Infection of humans by the larval stage of the tapeworms Echinococcus granulosus sensu lato or Echinococcus multilocularis causes the life-threatening zoonoses cystic echinococcosis (CE) and alveolar echinococcosis (AE). Although cystic liver lesions are a hallmark of both diseases, course, prognosis, and patients' management decisively differ between the two. The wide and overlapping spectrum of morphologies and the limited availability of ancillary tools are challenges for pathologists to reliably diagnose and subtype echinococcosis. Here, we systematically and quantitatively recorded the pathologic spectrum in a clinically and molecularly defined echinococcosis cohort (138 specimens from 112 patients). Immunohistochemistry using a novel monoclonal antibody (mAbEmG3) was implemented, including its combined application with the mAbEm2G11. Six morphologic criteria sufficiently discriminated between CE and AE: size of smallest (CE/AE: >2/≤2 mm) and largest cyst (CE/AE: >25/≤25 mm), thickness of laminated layer (CE/AE: >0.15/≤0.15 mm) and pericystic fibrosis (CE/AE: >0.6/≤0.6 mm), striation of laminated layer (CE/AE: moderate-strong/weak), and number of cysts (CE/AE: ≤9/>9). Combined immunohistochemistry with mAbEm2G11 (E. multilocularis specific) and mAbEmG3 (reactive in AE and CE) was equally specific as and occasionally more sensitive than polymerase chain reaction. On the basis of these findings, we developed a diagnostic algorithm for the differential diagnosis of echinococcosis. In summary, we have not only identified the means to diagnose echinococcosis with greater certainty, but also defined morphologic criteria, which robustly discriminate between CE and AE. We expect our findings to improve echinococcosis diagnostics, especially of challenging cases, beneficially impacting the management of echinococcosis patients

Serum glial fibrillary acidic protein in natalizumab-treated relapsing-remitting multiple sclerosis: An alternative to neurofilament light

BACKGROUND: There is a need in Relapsing-Remitting Multiple Sclerosis (RRMS) treatment for biomarkers that monitor neuroinflammation, neurodegeneration, treatment response, and disease progression despite treatment. OBJECTIVE: To assess the value of serum glial fibrillary acidic protein (sGFAP) as a biomarker for clinical disease progression and brain volume measurements in natalizumab-treated RRMS patients. METHODS: sGFAP and neurofilament light (sNfL) were measured in an observational cohort of natalizumab-treated RRMS patients at baseline, +3, +12, and +24 months and at the last sample follow-up (median 5.17 years). sGFAP was compared between significant clinical progressors and non-progressors and related to magnetic resonance imaging (MRI)-derived volumes of the whole brain, ventricle, thalamus, and lesion. The relationship between sGFAP and sNfL was assessed. RESULTS: sGFAP and neurofilament light (sNfL) were measured in an observational cohort of natalizumab-treated RRMS patients at baseline, +3, +12, and +24 months and at the last sample follow-up (median 5.17 years). sGFAP was compared between significant clinical progressors and non-progressors and related to magnetic resonance imaging (MRI)-derived volumes of the whole brain, ventricle, thalamus, and lesion. The relationship between sGFAP and sNfL was assessed. DISCUSSION: sGFAP levels related to MRI markers of neuroinflammation and neurodegeneration