Microrheology imaging of fiber suspensions – a case study for lyophilized collagen I in HCl solutions

In fiber suspensions with low optical contrast, the in situ characterization of structural properties with conventional microscopy methods fails. However, overlaying subsequent images of multiple particle tracking (MPT) videos including short trajectories usually discarded in MPT analysis allowed for direct visualization of individual fibers and the network structure of lyophilized collagen I (Coll) distributed in hydrochloric acid solutions. MPT yielded a broad distribution of mean square displacements (MSDs). Freely diffusing tracer particles yielded viscosities indicating that, irrespective of concentration, a constant amount of Coll is dissolved in the aqueous phase. Particles found elastically trapped within fibrous Coll structures exhibited a broad range of time-independent MSDs and we propose a structure comprising multiple fiber bundles with dense regions inaccessible to tracers and elastic regions of different stiffness in between. Bulky aggregates inaccessible to the 0.2 μm tracers exist even at low Coll concentrations, a network of slender fibers evolves above the sol–gel transition and these fibers densify with increasing Coll concentration. This novel MPT-based imaging technique possesses great potential to characterize the fiber distribution in and structural properties of a broad range of biological and technical suspensions showing low contrast when imaged with conventional techniques. Thus, MPT imaging and microrheology will help to better understand the effect of fiber distribution and network structure on the viscoelastic properties of complex suspensions

Ups and downs of a transcriptional landscape shape iron deficiency associated chlorosis of the maize inbreds B73 and Mo17

BACKGROUND: Improving nutrient homeostasis is a major challenge of a sustainable maize cultivation, and cornerstone to ensure food supply for a growing world population. Although, iron constitutes an important nutrient, iron availability is limited. In this respect, iron deficiency associated chlorosis causes severe yield losses every year. Natural variation of the latter trait has yet not been addressed in maize and was therefore studied in the present analysis. RESULTS: In this study, we i) report about the contrasting chlorosis phenotypes of the inbreds B73 and Mo17 at 10 and 300 μM iron regime, ii) identified over 400 significantly regulated transcripts (FDR < 0.05) within both inbreds at these growth conditions by deep RNA-Sequencing, iii) linked the gained knowledge with QTL information about iron deficiency related traits within the maize intermated B73 by Mo17 (IBM) population, and iv) highlighted contributing molecular pathways. In this respect, several genes within methionine salvage pathway and phytosiderophore synthesis were found to present constitutively high expression in Mo17, even under sufficient iron supply. Moreover, the same expression pattern could be observed for two putative bHLH transcription factors. In addition, a number of differentially expressed genes showed a co-localisation with QTL confidence intervals for iron deficiency related traits within the IBM population. CONCLUSIONS: Our study highlights differential iron deficiency associated chlorosis between B73 and Mo17 and represents a valuable resource for differentially expressed genes upon iron limitation and chlorosis response. Besides identifying two putative bHLH transcription factors, we propose that methionine salvage pathway and sterol metabolism amongst others; underlie the contrasting iron deficiency related chlorosis phenotype of both inbreds. Altogether, this study emphasizes a contribution of selected genes and pathways on natural trait variation within the IBM population

Fiche technique : Hoplocampe du pommier en AB

Le contrôle des ravageurs dits secondaires pré-occupe de plus en plus les arboriculteurs. La plus grande sélectivité des méthodes de protection phytosanitaire utilisées explique en partie l’augmentation de leur présence. Dans le contexte phytosanitaire actuel, il est primordial de caractériser les risques et d’identifier les solutions disponibles pour contrôler ces ravageurs. L’hoplocampe du pommier est un ravageur largement répandu en Europe et en Amérique du Nord. En verger de pommier conduit en AB, il peut entrainer de très fortes pertes de rende-ment préjudiciables économiquement. D’autres espèces d’hoplocampes s’attaquent à la poire (H. brevis) et à la prune (H. minuta et H. flava), elles ne sont pas traitées dans cette fiche

The European Registered Toxicologist (ERT) : Current status and prospects for advancement

Acknowledgements We would like to thank the participants of the five workshops in which the issues presented in this paper were discussed and the revised guidelines prepared, as well as the EUROTOX Executive Committee and the societies of toxicology of Sweden, the Netherlands, Switzerland, Austria and France for their support which allowed the workshops to take place.Peer reviewedPostprin

Fiche technique : Anthonome du pommier en AB

Le contrôle des ravageurs dits secondaires pré-occupe de plus en plus les arboriculteurs. La plus grande sélectivité des méthodes de protection phytosanitaire utilisées explique en partie l’augmentation de leur présence. Dans le contexte phytosanitaire actuel, il est primordial de caractériser les risques et d’identifier les solutions disponibles pour contrôler ces ravageurs.L’anthonome du pommier est un ravageur dont l’intensité des dégâts en verger a augmenté au cours des dernières décennies dans de nombreuses régions en Europe. Plusieurs travaux rapportent une intensité des dégâts pouvant atteindre 90%, remettant en cause la rentabilité économique à moyen terme de la culture

Spatial and seasonal variation in reproductive indices of the clupeids Limnothrissa miodon and Stolothrissa tanganicae in the Congolese waters of northern Lake Tanganyika

publishedVersio

Assessment of diseases susceptibility of peach cultivars in experimental plots and on-farm for organic and low-input systems. Baseline of French case studies

Despite a high turn-over of new peach cultivars, their suitability for organic and low-input systems remains unknown for most of them. Diseases susceptibility is an important criteria to consider since diseases control is a bottleneck to peach production in theses systems. Since 2001, 81 peach cultivars were assessed in 2 experimental sites and 7 on-farm plots

Screening Of Alternatives To Decrease Copper Dependency For Plasmopara Viticola Control In Organic Grape Production

Downy mildew caused by Plasmopara viticola is a major pest in viticulture worldwide. Organic viticulture is highly dependent of copper-based product to control this disease. Because copper has negative environmental impact, strategies to decrease dependency to copper-based products are needed. In that context, during 2005-2017 period, we have assessed the efficacy of 35 compounds to control downy mildew on-farm. This study present a selection of our results. The screening showed that several formulated products and compounds have an efficacy to control the downy mildew when they are compared with the untreated control, but none at an efficiency similar to copper. When alternatives compounds are associated with a low copper dose, significant additional effect are rarely observed over the whole season

Putative role of arthropod vectors in African swine fever virus transmission in relation to their bio-ecological properties

African swine fever (ASF) is one of the most important diseases in Suidae due to its significant health and socioeconomic consequences and represents a major threat to the European pig industry, especially in the absence of any available treatment or vaccine. In fact, with its high mortality rate and the subsequent trade restrictions imposed on affected countries, ASF can dramatically disrupt the pig industry in afflicted countries. In September 2018, ASF was unexpectedly identified in wild boars from southern Belgium in the province of Luxembourg, not far from the Franco-Belgian border. The French authorities rapidly commissioned an expert opinion on the risk of ASF introduction and dissemination into metropolitan France. In Europe, the main transmission routes of the virus comprise direct contact between infected and susceptible animals and indirect transmission through contaminated material or feed. However, the seasonality of the disease in some pig farms in Baltic countries, including outbreaks in farms with high biosecurity levels, have led to questions on the possible involvement of arthropods in the transmission of the virus. This review explores the current body of knowledge on the most common arthropod families present in metropolitan France. We examine their potential role in spreading ASF—by active biological or mechanical transmission or by passive transport or ingestion—in relation to their bio-ecological properties. It also highlights the existence of significant gaps in our knowledge on vector ecology in domestic and wild boar environments and in vector competence for ASFV transmission. Filling these gaps is essential to further understanding ASF transmission in order to thus implement appropriate management measures

Bioaffinity sensor based on nanoarchitectonic films: control of the specific adsorption of proteins through the dual role of an ethylene oxide spacer.

The identification and quantification of biomarkers or proteins is a real challenge in allowing the early detection of diseases. The functionalization of the biosensor surface has to be properly designed to prevent nonspecific interactions and to detect the biomolecule of interest specifically. A multilayered nanoarchitecture, based on polyelectrolyte multilayers (PEM) and the sequential immobilization of streptavidin and a biotinylated antibody, was elaborated as a promising platform for the label-free sensing of targeted proteins. We choose ovalbumin as an example. Thanks to the versatility of PEM films, the platform was built on two types of sensor surface and was evaluated using both optical- and viscoelastic-based techniques, namely, optical waveguide lightmode spectroscopy and the quartz crystal microbalance, respectively. A library of biotinylated poly(acrylic acids) (PAAs) was synthesized by grafting biotin moieties at different grafting ratios (GR). The biotin moieties were linked to the PAA chains through ethylene oxide (EO) spacers of different lengths. The adsorption of the PAA-EOn-biotin (GR) layer on a PEM precursor film allows tuning the surface density in biotin and thus the streptavidin adsorption mainly through the grafting ratio. The nonspecific adsorption of serum was reduced and even suppressed depending on the length of the EO arms. We showed that to obtain an antifouling polyelectrolyte the grafting of EO9 or EO19 chains at 25% in GR is sufficient. Thus, the spacer has a dual role: ensuring the antifouling property and allowing the accessibility of biotin moieties. Finally, an optimized platform based on the PAA-EO9-biotin (25%)/streptavidin/biotinylated-antibody architecture was built and demonstrated promising performance as interface architecture for bioaffinity sensing of a targeted protein, in our case, ovalbumin.journal articleresearch support, non-u.s. gov't2013 Jun 182013 02 11importe