Avaliação e redesenho da usabilidade e da acessibilidade do Sítio Web da ESTIG

Este artigo pretende descrever os processos de avaliação e de redesenho de um sítio web da administração pública portuguesa. Este trabalho teve como motivação focar a informação e os serviços oferecidos nas necessidades dos seus utilizadores alvo, tendo em atenção requisitos específicos que um sítio da administração pública portuguesa deve respeitar em diferentes domínios. Para tal realizou-se uma avaliação do anterior sítio web, dividida em 4 diferentes fases: (i) verificação da adopção de boas práticas de desenho de sítios web da administração pública; (ii) verificação de padrões universais de desenho de interfaces web; (iii) conformidade com os padrões de acessibilidade para pessoas com necessidades especiais WCAG1.0 e (iv) testes com utilizadores. Na sequência dos resultados desta avaliação foi desenvolvido um documento de recomendações de redesenho, que após várias iterações de decisão passou a uma fase de implementação. O sítio web redesenhado foi recentemente colocado em funcionamento (www.estig.ipbeja.pt). O artigo poderá fornecer ajuda metodológica e técnica, aos membros da administração pública que pretendam desenhar ou redesenhar um sítio web da administração pública

Ansiedad entre cuidadores de pacientes con Enfermedad Pulmonar Obstructiva Crónica tras el alta hospitalaria

Objective: To identify the factors that influence changes in caregivers anxiety status three months after discharge for acute exacerbation of Chronic Obstructive Pulmonary Disease (COPD).Methodology: Longitudinal study. Participants included 87 caregivers of patients hospitalized for acute exacerbation of COPD. Anxiety was measured at the time of hospitalization and three months after discharge. We measured factors from four domains: context of care, caregiving demands, caregiver resources, and patient characteristics. We used multiple univariate and multivariate logistic regressions to determine changes in anxiety three months later. Univariate and multivariate multiple logistic regressions were used to determine changes in anxiety three months later.Results: A total of 57.5% of caregivers reported anxiety at the time of hospitalization. Of these, 44% had a remission of their anxiety three months after discharge. However, 22% of caregivers who had not experienced anxiety at the hospitalization became anxious at 3 months. The severity of COPD and not receiving help from another caregiver decreased the likelihood of remission of anxiety. Moderately high overload increases the likelihood of experiencing anxiety symptoms.Conclusions: The perception of anxious symptoms is dynamic. Caregivers are likely to recover from anxiety when they receive help from another caregiver and if the patient they are caring for does not have severe COPD.Objetivo: Identificar los factores que influyen en cambios en la ansiedad de los cuidadores tres meses después del alta hospitalaria por exacerbación aguda de la Enfermedad Pulmonar Obstructiva Crónica (EPOC).  Metodología: Estudio longitudinal. Participaron 87 cuidadores de pacientes hospitalizados por exacerbación aguda de EPOC. Se midió la ansiedad en el momento de la hospitalización y tres meses después del alta. Además, se midieron potenciales factores asociados a su cambio en cuatro dominios: Contexto del cuidado, demandas del cuidado, recursos y características del paciente. Utilizamos regresiones logísticas múltiples univariadas y multivariadas para determinar los cambios en la ansiedad tres meses después. Resultados: Presentaron ansiedad en el momento de la hospitalización el 57,5% de los cuidadores. De ellos, el 44% había remitido su ansiedad tres meses después del alta hospitalaria. Sin embargo, el 22% de los cuidadores quienes no habían presentado ansiedad en el momento de la hospitalización se mostraron ansiosos a los 3 meses. La gravedad de la EPOC y no recibir apoyo de otro cuidador disminuyó las probabilidades de remisión de la ansiedad. La sobrecarga moderadamente alta incrementa las probabilidades de presentar  nuevos síntomas de ansiedad. Conclusiones: La percepción de los síntomas de la ansiedad es dinámica. Los cuidadores pueden recuperarse si reciben ayuda de otro cuidador o si el paciente al que cuidan no está en un estado severo de EPOC

Virtualidad: ¿positivo o negativo para el aprendizaje?

Introducción: En tiempos de pandemia el sistema educativo ha tenido que transformarse de manera imprevista a una modalidad virtual, la cual no era la habitual. Por este motivo, se quiere investigar si el proceso de enseñanza/aprendizaje on-line tiene el mismo impacto que el tradicional en forma presencial, y cuáles son las ventajas y desventajas que presentan los alumnos a la hora de estudiar. Esta idea surgió por la expectativa de comenzar un año facultativo de una manera que no se pudo llevar a cabo y también por el cambio rotundo de los estudiantes en su vida cotidiana para ajustarse a la emergencia sanitaria. Inevitablemente, la pérdida de socialización que forma parte de la experiencia cotidiana de un estudiante en educación superior podría tener sus consecuencias. Objetivos: Se quiere demostrar como el estudiante trasciende su año facultativo en esta situación y si en verdad afecta en su aprendizaje.Facultad de Odontologí

Ruthenium(II) Tris(2,2'-bipyridyl) Complex Incorporated in UiO-67 as Photoredox Catalyst

This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry C, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/acs.jpcc.8b07204[EN] Ruthenium(II) bis(2,2'-bipyridine) (2,2'-bipyridyl-5,5'-dicarboxylic acid), Rudcbpy, attached to the UiO-67(Zr) metal organic framework (MOF), generates upon visible light excitation the localized triplet excited state of the ruthenium(II) bipyridyl complex that decays partly to a very long-lived (millisecond time scale) photoinduced charge-separated state following a similar behavior as that of analogous ruthenium(II) tris(bipyridyl) complex dissolved in water, except that the lifetime of the soluble complex excited state is orders of magnitude shorter. The occurrence of photoinduced charge separation on the Rudcbpy-UiO-67(Zr) MOF has been proven visually by using methyl viologen as electron acceptor. Rudcbpy-UiO-67(Zr) behaves as a heterogeneous, reusable, photoredox catalyst promoting debromination of alpha-bromoketones with excellent selectivity at high conversions. Characterization data show that Rudcbpy-UiO-67(Zr) is stable under the photocatalytic reaction conditions.Financial support by the Spanish Ministry of Economy and Competitiveness (CTQ2014-53292-R-AR, Severo Ochoa, CTQ2015-69153-CO2-R) and Generalitat Valenciana (Prometeo 2017/083) is gratefully acknowledged. S.N. thanks the financial support by Fundacion Ramon Areces (XVIII Concurso Nacional, Ciencias de la Vida y de la Materia, Energia renovable: materiales y procesos 2016).Santiago-Portillo, A.; Garcia-Baldovi, H.; Carbonell Llopis, EJ.; Navalón Oltra, S.; Alvaro Rodríguez, MM.; García Gómez, H.; Ferrer Ribera, RB. (2018). Ruthenium(II) Tris(2,2'-bipyridyl) Complex Incorporated in UiO-67 as Photoredox Catalyst. The Journal of Physical Chemistry C. 122(51):29190-29199. https://doi.org/10.1021/acs.jpcc.8b07204S29190291991225

Non-carious cervical lesions in patients aged 19 to 59 years

Introducción: las lesiones cervicales no cariosas, son la pérdida patológica de la estructura dentaria localizada en el límite amelo-cementario, que no responden a un agente causal bacteriano.Objetivo: describir las lesiones cervicales no cariosas en Historias Clínicas de pacientes atendidos en el servicio de Periodoncia de La Clínica Estomatológica Docente Municipal de 19 a 59 años.Métodos: se realizó una investigación observacional descriptivo retrospectivo en el período de enero a diciembre del 2019. La población estuvo constituida por todas las Historias Clínicas de pacientes de 19 a 59 años ascendente a 1063 que acudieron al servicio de Periodoncia, la muestra quedo conformada por 233 Historias Clínicas que tenían diagnóstico de Lesiones cervicales no cariosas en ese período. Las variables que se utilizaron fueron: la edad, sexo, lesiones cervicales no cariosas, factores de riesgo, hábitos lesivos, cepillado traumático, dieta ácida, estrés, bruxismo y grupos dentarios.Resultados: el grupo de edad más representativo fue el de 50 a 59 años y el sexo femenino con 51,1%, la abrasión con un 40,3 %. Predominaron los grupos dentarios de los premolares con un 45,5 % y los factores de riesgo de cepillado traumático y el bruxismo con el 30,0 % y 18,9 % respectivamente.Conclusiones: predominó el grupo etario de 50 a 59 años y el sexo femenino. El grupo dentario más afectados fueron los premolares. De los tipos de lesiones cervicales no cariosas la más frecuente fue la abrasión y el cepillado traumático fue el factor de riesgo de mayor aparición. Introduction: non-carious cervical lesions are the pathological loss of the dental structure located in the amelo-cementary limit, which do not respond to a bacterial causal agent.Objective: to describe the non-carious cervical lesions in the clinical histories of patients attended at the Periodontics Service of the Clínica Estomatológica Docente Municipal from 19 to 59 years old.Methods: a retrospective descriptive observational research was carried out from January to December 2019. The population was constituted by all the Clinical Histories of patients from 19 to 59 years old, amounting to 1063 that attended the Periodontics service, the sample was conformed by 233 Clinical Histories that had a diagnosis of non-carious cervical lesions in that period. The variables used were: age, sex, non-carious cervical lesions, risk factors, injurious habits, traumatic brushing, acid diet, stress, bruxism and dental groups. Results. The most representative age group was 50 to 59 years and female  sex    with 51,1 %,   abrasion with 40,3 %. The premolar tooth groups predominated with 45,5 % and the risk factors of traumatic brushing and bruxism with 30,0 % and 18,9 % respectively.Conclusions: the age group 50 to 59 years and the female sex predominated. The most affected dental group was the premolars. Of the types of non-carious cervical lesions, the most frequent was abrasion and traumatic brushing was the risk factor of greatest occurrence

Hypermethylated 14-3-3-σ and ESR1 gene promoters in serum as candidate biomarkers for the diagnosis and treatment efficacy of breast cancer metastasis

Background: Numerous hypermethylated genes have been reported in breast cancer, and the silencing of these genes plays an important role in carcinogenesis, tumor progression and diagnosis. These hypermethylated promoters are very rarely found in normal breast. It has been suggested that aberrant hypermethylation may be useful as a biomarker, with implications for breast cancer etiology, diagnosis, and management. The relationship between primary neoplasm and metastasis remains largely unknown. There has been no comprehensive comparative study on the clinical usefulness of tumor-associated methylated DNA biomarkers in primary breast carcinoma and metastatic breast carcinoma. The objective of the present study was to investigate the association between clinical extension of breast cancer and methylation status of Estrogen Receptor1 (ESR1) and Stratifin (14-3-3-σ) gene promoters in disease-free and metastatic breast cancer patients. Methods: We studied two cohorts of patients: 77 patients treated for breast cancer with no signs of disease, and 34 patients with metastatic breast cancer. DNA was obtained from serum samples, and promoter methylation status was determined by using DNA bisulfite modification and quantitative methylation-specific PCR. Results: Serum levels of methylated gene promoter 14-3-3-σ significantly differed between Control and Metastatic Breast Cancer groups (P < 0.001), and between Disease-Free and Metastatic Breast Cancer groups (P < 0.001). The ratio of the 14-3-3-σ level before the first chemotherapy cycle to the level just before administration of the second chemotherapy cycle was defined as the Biomarker Response Ratio [BRR]. We calculated BRR values for the "continuous decline" and "rise-and-fall" groups. Subsequent ROC analysis showed a sensitivity of 75% (95% CI: 47.6 - 86.7) and a specificity of 66.7% (95% CI: 41.0 - 86.7) to discriminate between the groups for a cut-off level of BRR = 2.39. The area under the ROC curve (Z = 0.804 ± 0.074) indicates that this test is a good approach to post-treatment prognosis. Conclusions: The relationship of 14-3-3-σ with breast cancer metastasis and progression found in this study suggests a possible application of 14-3-3-σ as a biomarker to screen for metastasis and to follow up patients treated for metastatic breast cancer, monitoring their disease status and treatment response.This study was supported by a grant from the Ministerio de Ciencia e Innovación: SAF 2004-00889; JL Linares is supported by the Junta de Andalucía (P06-CTS-1385)

Educación ambiental y sociedad. Saberes locales para el desarrollo y la sustentabilidad

EL LIBRO PERMITE REFLEXIONAR SOBRE LA IMPORTANCIA DE FOMENTAL LA EDUCACIÓN AMBIENTAL PARA RESOLVER LA PROBLEMÁTICA AMBIENTALEL LIBRO PRESENTA DIFERENTES TRABAJOS QUE ESTUDIAN EL TEMA D ELA SUSTENTABILIDAD, ENFATIZANDO LA IMPORTANCIA DE LA EDUCACIÓN AMBIENTAL Y LA TRANSDISCIPLINANINGUN

Data from: Wolbachia effects in natural populations of Chorthippus parallelus from the Pyrenean hybrid zone.

We evaluate for the first time the effect of Wolbachia infection, involving two different supergoups, on the structure and dynamics of the hybrid zone between two subspecies of Chorthippus parallelus (Orthoptera) in the Pyrenees. Wolbachia infection showed no effects on female fecundity or a slight increment in females infected by F supergroup although in the last case it has to be well-established. Cytoplasmic incompatibility (CI) is confirmed in crosses carried out in the field between individuals from a natural hybrid population. This CI, registered as the relative reduction in embryo production (sh), was of sh = 0.355 and sh = 0.286 in unidirectional crosses involving B and F supergroups, respectively. CI also occurred in bidirectional crosses (sh = 0.147) but with a weaker intensity. The transmission rates of the two Wolbachia strains (B and F) were estimated by the optimization of a theoretical model in order to reach the infection frequencies observed in certain population. To fit this scenario both supergroups should present transmission rates close to 1. Further, we have simulated the infection dynamics, and hence the capacity of Wolbachia to structure the population of the host insects, and to affect to reproduction and genetic introgression in the hybrid zone. This represents a first example of the influence of Wolbachia in an insect natural hybrid zone

Lactic acid production using cheese whey based medium in a stirred tank reactor by a ccpA mutant of Lacticaseibacillus casei

In lactobacilli, CcpA is known to modulate the expression of genes involved in sugar metabolism, stress response and aerobic adaptation. This study aimed to evaluate a ccpA mutant of Lacticaseibacillus casei BL23 to increase lactic acid production using cheese whey. The ccpA derivative (BL71) showed better growth than the L. casei wild-type in the whey medium. In a stirred tank reactor, at 48 h, lactate production by BL71 was eightfold higher than that by BL23. In batch fermentations, the final values reached were 44.23 g L⁻¹ for BL71 and 27.58 g L⁻¹ for BL23. Due to a decrease in the delay of lactate production in the mutant, lactate productivity increased from 0.17 g (L.h)⁻¹ with BL23 to 0.80 g (L.h)⁻¹ with BL71. We found that CcpA would play additional roles in nitrogen metabolism by the regulation of the proteolytic system. BL71 displayed higher activity of the PepX, PepQ and PrtP enzymes than BL23. Analysis of prtP expression confirmed this deregulation in BL71. Promoter analysis of the prtP gene revealed CcpA binding sites with high identity to the cre consensus sequence and the interaction of CcpA with this promoter was confirmed in vitro. We postulate that deregulation of the proteolytic system in BL71 allows a better exploitation of nitrogen resources in cheese whey, resulting in enhanced fermentation capacity. Therefore, the ccpA gene could be a good target for future technological developments aimed at effective and inexpensive lactate production from dairy industrial wastes.Fil: Catone, Mariela Verónica. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; ArgentinaFil: Palomino, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Legisa, Danilo Mario. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fina Martin, Joaquina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Monedero Garcia V.. Consejo Superior de Investigaciones Científicas; EspañaFil: Ruzal, Sandra Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Allievi, Mariana Caludia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin