Realisation of Service-Oriented Architecture: Web Services

Tématem diplomové práce je architektura orientovaná na služby (SOA). Zprostředkovává celkový přehled webových služeb jako realizace SOA. Práce zmiňuje, co je připraveno a co schází k naplnění základních principů SOA. Analyzuje nástroje platformy Java EE pro webové služby. Představuje technologii Java Management Extensions (JMX) pro řešení jedné z oblastí webových služeb - SOA governance. Analyzuje možnosti použití JMX pro řízení a monitorování webových služeb. Za účelem testování implementuje základní aplikaci pro řízení a monitorování webových služeb na serveru GlassFish.The main subject of this master thesis is the Service-Oriented Architecture (SOA). It shows a complete overview of Web Services in terms of realisation of a SOA. The thesis mentions what is already done and what else remains to ful ll the basic SOA principles. Then, it reviews Java EE platform tools used to deal with the Web Services. Next, it introduces the Java Management Extensions (JMX) technology as a solution of the web service's area - SOA governance. Moreover, it examinates the possible usage of JMX for the web service's management and monitoring issues. Finally, it implements an exemplary web services management and monitoring application on GlassFish server for testing purposes.

Geotechnical Geocaching

Import 26/06/2013V mé bakalářské práci se budu zabývat spojením dvou zdánlivě neslučitelných fenoménů, a to Geotechniky a Geocachingu. Tedy oborů, které mají rozdílnou rychlost v získávání popularity u občanů. Teoretická část práce je zaměřena na stručnou charakteristiku obou disciplín, jejich historii a význam v praxi. Praktická část je rozdělena do čtyř částí, a to zcela samostatně uvažovaná Katedra geotechniky a podzemního stavitelství na Fakultě stavební VŠB - TUO; dále pak podzemní stavby, základové konstrukce a ostatní geotechnické lokality či objekty. V této části se již věnuji konkrétním geotechnicky zajímavým objektům, nalézajícím se v Moravskoslezském kraji, a především jejich geotechnické specifikaci a cache údajům potřebným k nalezení lokality. Na konci těchto jednotlivých kapitol se nachází cache údaje potřebné k nalezení lokality a fotodokumentace.My bachelor’s dissertation is dedicated to Geotechnics and Geocaching. Both seemingly incompatible fields with different popularity increase within their industries. The theoretical part of my bachelor’s dissertation is focused on a brief review of geotechnical and Geocaching, their history and significance in practice. The practical part is divided into four parts. It mentioned Department of Geotechnical and Underground Engineering at the Faculty of Civil Engineering Technical University Ostrava independently. Further it mentioned underground construction, foundation engineering and geotechnical locations or objects. This section has been devoted to the specific geotechnically interesting objects across the territory, North Moravia Region, and especially their geotechnical specification, cache data to find the location and the photographic documentation.Prezenční224 - Katedra geotechniky a podzemního stavitelstvívýborn

Prefabricated container house in the passive standard.

Import 26/02/2015Cílem diplomové práce s názvem Prefabrikovaný kontejnerový dům v pasivním standardu bylo zpracování textové a výkresové dokumentace pro zadaný objekt. Práce je složena z části stavebně a tepelně technické, studie denního osvětlení a bilance zasklení, akustického zhodnocení, části vytápění a větrání, návrhu vnitřní kanalizace a ekonomického zhodnocení jednotlivých částí. Stavba byla navržena tak, aby vyhověla požadavkům a principům pasivní výstavby. Snaha byla o co nejjednodušší konstrukci, která by splňovala nejvyšší požadavky na rychlost, současně však pevnost, trvanlivost a následnou recyklaci výstavby a která by byla současně ekonomicky dostupná v rámci pasivního standardu. Tento požadavek ocelová konstrukce splňuje. Celý projekt je pojat jako industriální stavba a jeho snahou je začlenění a rozšíření podobného druhu prefabrikované rychlé výstavby do standardu rodinného bydlení.The aim of my thesis titled as The Prefabricated Container House in the passive standard was to create technical documentation including technical description and drawings for the specified object. The work consists of construction of a thermal technical study of daylighting and glazing, sound appreciation of heating and ventilation, the design of inner sewerage and evaluation of the individual parts. The building was designed to meet the requirements and principles of passive construction. The goal was the simplest design that would meet the highest requirements such as rapid construction time, strength, durability, subsequent recycling of construction and the passive standard within low budget. All those requirements are met. The project is conceived as an industrial building. Its goal is integration and extension of a similar project type with rapid construction for the standard family housing.Prezenční229 - Katedra prostředí staveb a TZBvýborn

Suggestions for changes in the production of a part "revolver trigger"

Studie pojednává o technologii výroby součásti „spoušť revolveru“. Finální sestavou je krátká ruční palná zbraň ALFA Steel a její modelové řady společnosti ALFA-PROJ spol. s r.o. V úvodu se studie zabývá spouští a její technologičností. Následuje seznámení s průběhem výroby podle současného technologického postupu a jejími nedostatky. Byla zjištěna hlavní příčina výrobní nepřesnosti a provedla se analýza návrhů změn včetně úpravy technologického postupu. Na základě experimentu se v technické kontrole měřily rozměry na poskytnutých vzorcích odlitků navržené výroby. V experimentu se ověřovaly i rozměry spouští vyrobených současnou výrobou. Výsledky naměřených hodnot se navzájem porovnaly a na základě shody produktů vyhodnotily. V konečné fázi studie se porovnaly výrobní náklady na práci jednoho kusu spouště s nově navrženou technologií.The study deals with production technology of “revolver trigger“. Final assembly is short firearm ALFA Steel and its model series of ALFA-PROJ, Ltd company. Introduction submits an explanation of trigger technology suitability. The following analysis describes current manufacturing process, which has manufacturing inaccuracy problem. Furthermore rootcause of manufacturing inaccuracy was determined and analysis of suggestions for changes (including modifications of technological process) was realized. Based on experiment dimensions were measured on provided samples of suggest production in Technical Control Department. Dimensions of triggers manufactured by current production were verified during the experiment too. Results were compared according to the rejects of production. In the final phase of the study manufacturing labor costs were compared for the one piece of trigger with the new suggested technology.

Mono-\u3cem\u3eN\u3c/em\u3e-acyl-2,6-diaminopimelic Acid Derivatives: Analysis by Electromigration and Spectroscopic Methods and Examination of Enzyme Inhibitory Activity

Thirteen mono-N-acyl derivatives of 2,6-diaminopimelic acid (DAP)—new potential inhibitors of the dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE; EC 3.5.1.18)—were analyzed and characterized by infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies and two capillary electromigration methods: capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC). Structural features of DAP derivatives were characterized by IR and NMR spectroscopies, whereas CZE and MEKC were applied to evaluate their purity and to investigate their electromigration properties. Effective electrophoretic mobilities of these compounds were determined by CZE in acidic and alkaline background electrolytes (BGEs) and by MEKC in acidic and alkaline BGEs containing a pseudostationary phase of anionic detergent sodium dodecyl sulfate (SDS) or cationic detergent cetyltrimethylammonium bromide (CTAB). The best separation of DAP derivatives, including diastereomers of some of them, was achieved by MEKC in an acidic BGE (500 mM acetic acid [pH 2.54] and 60 mM SDS). All DAP derivatives were examined for their ability to inhibit catalytic activity of DapE from Haemophilus influenzae (HiDapE) and ArgE from Escherichia coli (EcArgE). None of these DAP derivatives worked as an effective inhibitor of HiDapE, but one derivative—N-fumaryl, Me-ester-DAP—was found to be a moderate inhibitor of EcArgE, thereby providing a promising lead structure for further studies on ArgE inhibitors

Converting Insulin-like Growth Factors 1 and 2 into High-Affinity Ligands for Insulin Receptor Isoform A by the Introduction of an Evolutionarily Divergent Mutation

Insulin-like growth factors 1 and 2 (IGF-1 and -2, respectively) are protein hormones involved not only in normal growth and development but also in life span regulation and cancer. They exert their functions mainly through the IGF-1R or by binding to isoform A of the insulin receptor (IR-A). The development of IGF-1 and IGF-2 antagonists is of great clinical interest. Mutations of A4 and A8 sites of human insulin lead to disproportionate effects on hormone IR binding and activation. Here, we systematically modified IGF-1 sites 45, 46, and 49 and IGF-2 sites 45 and 48, which correspond, or are close, to insulin sites A4 and A8. The IGF-1R and IR-A binding and autophosphorylation potencies of these analogues were characterized. They retained the main IGF-1R-related properties, but the hormones with His49 in IGF-1 and His48 in IGF-2 showed significantly higher affinities for IR-A and for IR-B, being the strongest IGF-1- and IGF-2-like binders of these receptors ever reported. All analogues activated IR-A and IGF-1R without major discrepancies in their binding affinities. This study revealed that IR-A and IGF-1R contain specific sites, likely parts of their so-called sites 2′, which can interact differently with specifically modified IGF analogues. Moreover, a clear importance of IGF-2 site 44 for effective hormone folding was also observed. These findings may facilitate novel and rational engineering of new hormone analogues for IR-A and IGF-1R studies and for potential medical applications

Computational and Structural Evidence for Neurotransmitter-mediated Modulation of the Oligomeric States of Human Insulin in Storage Granules

Human insulin is a pivotal protein hormone controlling metabolism, growth and ageing, and whose malfunctioning underlies diabetes, some cancers and neuro-degeneration. Despite its central position in human physiology, the in vivo oligomeric state and conformation of insulin in its storage granules in the pancreas are not known. In contrast, many in vitro structures of hexamers of this hormone are available, which fall into three conformational states: T6, T3Rf3 and R6. As there is strong evidence for accumulation of neurotransmitters, such as serotonin and dopamine, in insulin storage granules in pancreatic β-cells, we probed by molecular dynamics (MD) and protein crystallography (PC) if these endogenous ligands affect and stabilize insulin oligomers. Parallel studies independently converged on the observation that serotonin binds well within the insulin hexamer (site I), stabilizing it in the T3R3 conformation. Both methods indicated serotonin binding on the hexamer surface (site III) as well. MD, but not PC, indicated that dopamine was also a good site III ligand. Some of the PC studies also included arginine, which may be abundant in insulin granules upon processing of pro-insulin, and stable T3R3 hexamers loaded with both serotonin and arginine were obtained. The MD and PC results were supported further by in solution spectroscopic studies with R-state specific chromophore. Our results indicate that the T3R3 oligomer is a plausible insulin pancreatic storage form, resulting from its complex interplay with neurotransmitters, and pro-insulin processing products. These findings may have implications for clinical insulin formulations

Insulin-like Growth Factor 1 Analogs Clicked in the C Domain : Chemical Synthesis and Biological Activities

Human insulin-like growth factor 1 (IGF-1) is a 70 amino acid protein hormone, with key impact on growth, development, and lifespan. The physiological and clinical importance of IGF-1 prompted challenging chemical and biological trials toward the development of its analogs as molecular tools for the IGF-1 receptor (IGF1-R) studies and as new therapeutics. Here, we report a new method for the total chemical synthesis of IGF-1 analogs, which entails the solid-phase synthesis of two IGF-1 precursor chains that is followed by the CuI-catalyzed azide-alkyne cycloaddition ligation and by biomimetic formation of a native pattern of disulfides. The connection of the two IGF-1 precursor chains by the triazole-containing moieties, and variation of its neighboring sequences (Arg36 and Arg37), was tolerated in IGF-1R binding and its activation. These new synthetic IGF-1 analogs are unique examples of disulfide bonds' rich proteins with intra main-chain triazole links. The methodology reported here also presents a convenient synthetic platform for the design and production of new analogs of this important human hormone with non-standard protein modifications

Probing Receptor Specificity by Sampling the Conformational Space of the Insulin-like Growth Factor II C-domain

Insulin and insulin-like growth factors I and II are closely related protein hormones. Their distinct evolution has resulted in different yet overlapping biological functions with insulin becoming a key regulator of metabolism, whereas insulin-like growth factors (IGF)-I/II are major growth factors. Insulin and IGFs cross-bind with different affinities to closely related insulin receptor isoforms A and B (IR-A and IR-B) and insulin-like growth factor type I receptor (IGF-1R). Identification of structural determinants in IGFs and insulin that trigger their specific signaling pathways is of increasing importance in designing receptor-specific analogs with potential therapeutic applications. Here, we developed a straightforward protocol for production of recombinant IGF-II and prepared six IGF-II analogs with IGF-I-like mutations. All modified molecules exhibit significantly reduced affinity toward IR-A, particularly the analogs with a Pro-Gln insertion in the C-domain. Moreover, one of the analogs has enhanced binding affinity for IGF-1R due to a synergistic effect of the Pro-Gln insertion and S29N point mutation. Consequently, this analog has almost a 10-fold higher IGF-1R/IR-A binding specificity in comparison with native IGF-II. The established IGF-II purification protocol allowed for cost-effective isotope labeling required for a detailed NMR structural characterization of IGF-II analogs that revealed a link between the altered binding behavior of selected analogs and conformational rearrangement of their C-domains