Aplicación de test con la aplicación Hangout para la asignatura de Biología Celular de 3º Biología

Memoria ID-010. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2018-2019

Mesenchymal stromal cells combined with elastin-like recombinamers increase angiogenesis in vivo after hindlimb ischemia

Producción CientíficaHindlimb ischemia is an unmet medical need, especially for those patients unable to undergo vascular surgery. Cellular therapy, mainly through mesenchymal stromal cell (MSC) administration, may be a potentially attractive approach in this setting. In the current work, we aimed to assess the potential of the combination of MSCs with a proangiogenic elastin-like recombinamer (ELR)–based hydrogel in a hindlimb ischemia murine model. Human bone marrow MSCs were isolated from four healthy donors, while ELR biomaterials were genetically engineered. Hindlimb ischemia was induced through ligation of the right femoral artery, and mice were intramuscularly injected with ELR biomaterial, 0.5 × 106 MSCs or the combination, and also compared to untreated animals. Tissue perfusion was monitored using laser Doppler perfusion imaging. Histological analysis of hindlimbs was performed after hematoxylin and eosin staining. Immunofluorescence with anti–human mitochondria antibody was used for human MSC detection, and the biomaterial was detected by elastin staining. To analyze the capillary density, immunostaining with an anti–CD31 antibody was performed. Our results show that the injection of MSCs significantly improves tissue reperfusion from day 7 (p = 0.0044) to day 21 (p = 0.0216), similar to the infusion of MSC + ELR (p = 0.0038, p = 0.0014), without significant differences between both groups. After histological evaluation, ELR hydrogels induced minimal inflammation in the injection sites, showing biocompatibility. MSCs persisted with the biomaterial after 21 days, both in vitro and in vivo. Finally, we observed a higher blood vessel density when mice were treated with MSCs compared to control (p<0.0001), but this effect was maximized and significantly different to the remaining experimental conditions when mice were treated with the combination of MSCs and the ELR biomaterial (p < 0.0001). In summary, the combination of an ELR-based hydrogel with MSCs may improve the angiogenic effects of both strategies on revascularization of ischemic tissues.Spanish Government (RTI2018-096320-B-C22, FPU16-04015, PID2019-110709RB-I00, PID2020-118669RA-I00)Interreg V España Portugal POCTEP (0624_2IQBIONEURO_6_E), Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y LeónConsejería de Educación de Castilla y León (CAS079P17)Instituto de Salud Carlos III (ISCIII) (PI19/01630)Programs of ISCIII- European Regional Development Fund (RD16/0011/0015, RD21/ 0017/0006

Effect of mTORC1/mTORC2 inhibition on T cell function: potential role in graft-versus-host disease control

Producción CientíficaThe mechanistic target of rapamycin (mTOR) pathway is crucial for the activation and function of T cells, which play an essential role in the development of graft-versus-host disease (GvHD). Despite its partial ability to block mTOR pathway, the mTORC1 inhibitor rapamycin has shown encouraging results in the control of GvHD. Therefore, we considered that simultaneous targeting of both mTORC1 and mTORC2 complexes could exert a more potent inhibition of T cell activation and, thus, could have utility in GvHD control. To assess this assumption, we have used the dual mTORC1/mTORC2 inhibitors CC214-1 and CC214-2. In vitro studies confirmed the superior ability of CC214-1 versus rapamycin to block mTORC1 and mTORC2 activity and to reduce T cell proliferation. Both drugs induced a similar decrease in Th1/Th2 cytokine secretion, but CC214-1 was more efficient in inhibiting na€ıve T cell activation and the expression of Tcell activation markers. In addition, CC214-1 induced specific tolerance against alloantigens, while preserving anti-cytomegalovirus response. Finally, in a mouse model of GvHD, the administration of CC214-2 significantly improved mice survival and decreased GvHD-induced damages. In conclusion, the current study shows, for the first time, the immunosuppressive ability of CC214-1 on T lymphocytes and illustrates the role of CC214-2 in the allogeneic transplantation setting as a possible GvHD prophylaxis agent.Gerencia Regional de Salud de Castilla y León (Proyecto GRS 726/A13

Diseño de una revista on line sobre noticias de investigaciones relevantes en Biotecnología en el campo de la Biología Celular y Tisular

Memoria ID-0010. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2017-2018

Ensayos formativos autoevaluables en asignaturas del área Biología Celular

Memoria ID12-0146. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2012-2013

Desarrollo de prácticas con realidad aumentada para las asignaturas del área de Biología Celular

Memoria ID-138. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2019-2020.[ES]El objetivo final del presente proyecto de innovación docente ha sido poner a punto la realidad aumentada (RA) mediante la utilización de imágenes y hologramas enlazados al sistema Merge Cube para que el alumno pueda analizar estructuras en 3D de los diferentes temas del Área de Biología celula

Talleres de olfacción y gusto: prácticas académicas y divulgativas de sistemas sensoriales químicos

Memoria ID-185. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014

Targeting of PI3K/AKT/mTOR pathway to inhibit T cell activation and prevent graft-versus-host disease development

Producción CientíficaBackground: Graft-versus-host disease (GvHD) remains the major obstacle to successful allogeneic hematopoietic stem cell transplantation, despite of the immunosuppressive regimens administered to control T cell alloreactivity. PI3K/AKT/mTOR pathway is crucial in T cell activation and function and, therefore, represents an attractive therapeutic target to prevent GvHD development. Recently, numerous PI3K inhibitors have been developed for cancer therapy. However, few studies have explored their immunosuppressive effect. Methods: The effects of a selective PI3K inhibitor (BKM120) and a dual PI3K/mTOR inhibitor (BEZ235) on human T cell proliferation, expression of activation-related molecules, and phosphorylation of PI3K/AKT/mTOR pathway proteins were analyzed. Besides, the ability of BEZ235 to prevent GvHD development in mice was evaluated. Results: Simultaneous inhibition of PI3K and mTOR was efficient at lower concentrations than PI3K specific targeting. Importantly, BEZ235 prevented naïve T cell activation and induced tolerance of alloreactive T cells, while maintaining an adequate response against cytomegalovirus, more efficiently than BKM120. Finally, BEZ235 treatment significantly improved the survival and decreased the GvHD development in mice. Conclusions: These results support the use of PI3K inhibitors to control T cell responses and show the potential utility of the dual PI3K/mTOR inhibitor BEZ235 in GvHD prophylaxis.Asociación Española Contra el Cáncer (Proyecto AIOA110296BLAN).Gerencia Regional de Salud de Castilla y León (Proyecto GRS 726/A13

Calbindin D-28k immunoreactivity in the rat accessory olfactory bulb

The distribution pattern and the morphology of calbindin D-28k-immunoreactive neurons were studied in the accessory olfactory bulb of the rat using a monoclonal antibody and the avidin-biotin-immunoperoxidase method. Positive neurons were observed in all layers but the vomeronasal nerve layer. Scarce monodendritic periglomerular neurons were calbindin D-28k immunoreactive. Different morphological types of short-axon cells were calbindin D-28k immunostained, with different degrees of intensity, in the boundary between the internal and external plexiform layer. In addition, deep short-axon cells located in the granule cell layer were calbindin D-28k-immunopositive. By contrast, previous studies described all cells in the rat accessory olfactory bulb as calbindin D-28kimmunonegative.The staining pattern in the rat accessory olfactory bulb showed both similarities and differences with the distribution pattern of the same calcium-binding protein in the main olfactory bulb

Neuroquímica del encéfalo : cd-rom de la organización y quimioarquitectura del cerebro

El proyecto se ha llevado acabo en el departamento de Biología Celular y Patología de la Universidad de Salamanca por un grupo de seis profesores. Se ha elaborado un cd-rom con un índice donde se describen los elementos con los que se ha construido, incluyendo: animales, técnicas empleadas, material utilizado y el diseño informático con el que se ha estructurado. En cuanto al contenido, el alumno va eligiendo entre niveles cada vez con más detalle; primero elige el nivel de corte dentro de las cinco grandes regiones encefálicas, después elige entre los distintos marcadores neuroquímicos utilizados y además puede elegir vistas panorámicas o detalles. El alumno puede también comparar la organización cerebral en condiciones normales y tras un proceso de neurodegeneración. Los objetivos del proyecto son: familiarizar al alumno con la organización neuroquímica del cerebro y facilitarle el conocimiento e identificación de regiones cerebrales, núcleos y poblaciones neuronales; mejorar la calidad de la enseñanza y la motivación del alumno; convertir al alumno en protagonista de su propio aprendizaje, permitiéndole hacer búsquedas comparar niveles; conseguir un nuevo recurso para las clases prácticas con más potencia, más flexibilidad y más económico; favorecer la integración entre diferentes asignaturas y distintas disciplinas. El cd-rom que se ha elaborado supone un método complementario a la enseñanza clásica. Se trata de recursos propios, flexibles y adaptables para la utilización en asignaturas de la universidad, seminarios conferencias y podrá ser compartido por diferentes profesores y utilizado de forma individualizada por el alumno.Junta de Castilla y León. Consejería de Educación y CulturaCastilla y LeónES