Modeling the response of top-down control exerted by gelatinous carnivores on the Black Sea pelagic food web

Recent changes in structure and functioning of the interior Black Sea ecosystem are studied by a series of simulations using a one-dimensional, vertically resolved, coupled physical-biochemical model. The simulations are intended to provide a better understanding of how the pelagic food web structure responds to increasing grazing pressure by gelatinous carnivores (medusae Aurelia aurita and ctenophore Mnemiopsis leidyi) during the past 2 decades. The model is first shown to represent typical eutrophic ecosystem conditions of the late 1970s and early 1980s. This simulation reproduces reasonably well the observed planktonic food web structure at a particular location of the Black Sea for which a year-long data set is available from 1978. Additional simulations are performed to explore the role of the Mnemiopsis-dominated ecosystem in the late 1980s. They are also validated by extended observations from specific years. The results indicate that the population outbreaks of the gelatinous species, either Aurelia or Mnemiopsis, reduce mesozooplankton grazing and lead to increased phytoplankton blooms as observed throughout the 1980s and 1990s in the Black Sea. The peaks of phytoplankton, mesozooplankton, Noctiluca, and gelatinous predator biomass distributions march sequentially as a result of prey-predator interactions. The late winter diatom bloom and a subsequent increase in mesozooplankton stocks are robust features common to all simulations. The autotrophs and heterotrophs, however, have different responses during the rest of the year, depending on the nature of grazing pressure exerted by the gelatinous predators. In the presence of Mnemiopsis, phytoplankton have additional distinct and pronounced bloom episodes during the spring and summer seasons. These events appear with a 2 month time shift in the ecosystem prior to introduction of Mnemiopsis

Predation and food limitation as causes of mortality in larval herring at a spawning ground in British Columbia

We quantified both in situ predation on Pacific herring (Clupea haregus pallasi ) larvae by soft-bodied zooplankton, and microzooplankton prey of herring larvae in Kulleet Bay, Vancouver Island, British Columbia. Samples were collected at 0 to 5 m depth daily at peak larval hatching from 14 to 21 April 1985. The hydromedusa Aequorea victoria was the only soft-bodied zooplankter that ate herring larvae. Densities of A. victoria reached as much as 17 m⁻³, and averaged 1 to 5 m⁻³. Predation on the herring larvae was severe, averaging 57 ± 29% d⁻¹ of larvae during each sampling period. Microzooplankton prey of post-yolksac herring larvae were mainly copepod nauplii and eggs, shelled protozoans, and bivalve veligers, and averaged 40.8 ± 21.51⁻¹ in the environment.Keywords: Clupea harengus pallasi, mortality causes, zooplankton, spawning ground, fish larva

Next Generation Practitioner-Scholars Navigating Community Engagement Professional Development: A Collaborative Autoethnography

This collaborative autoethnographic research study examines the motivations, experiences, and professional outcomes of seven community engagement practitioner-scholars who served in a high-level elected position in a community engagement research association and its affiliated graduate student network. The findings highlight the role of professional associations and graduate student networks in facilitating professional development for next generation community engagement practitioner-scholars, such as supporting them in creating connections, expanding networks, developing professional identities, and cultivating cultural capital

Deterministic Factors Overwhelm Stochastic Environmental Fluctuations as Drivers of Jellyfish Outbreaks

16 pages, 4 figures, 1 table, supporting Information http://dx.doi.org/10.1371/journal.pone.0141060Jellyfish outbreaks are increasingly viewed as a deterministic response to escalating levels of environmental degradation and climate extremes. However, a comprehensive understanding of the influence of deterministic drivers and stochastic environmental variations favouring population renewal processes has remained elusive. This study quantifies the deterministic and stochastic components of environmental change that lead to outbreaks of the jellyfish Pelagia noctiluca in the Mediterranen Sea. Using data of jellyfish abundance collected at 241 sites along the Catalan coast from 2007 to 2010 we: (1) tested hypotheses about the influence of time-varying and spatial predictors of jellyfish outbreaks; (2) evaluated the relative importance of stochastic vs. deterministic forcing of outbreaks through the environmental bootstrap method; and (3) quantified return times of extreme events. Outbreaks were common in May and June and less likely in other summer months, which resulted in a negative relationship between outbreaks and SST. Cross- and along-shore advection by geostrophic flow were important concentrating forces of jellyfish, but most outbreaks occurred in the proximity of two canyons in the northern part of the study area. This result supported the recent hypothesis that canyons can funnel P. noctiluca blooms towards shore during upwelling. This can be a general, yet unappreciated mechanism leading to outbreaks of holoplanktonic jellyfish species. The environmental bootstrap indicated that stochastic environmental fluctuations have negligible effects on return times of outbreaks. Our analysis emphasized the importance of deterministic processes leading to jellyfish outbreaks compared to the stochastic component of environmental variation. A better understanding of how environmental drivers affect demographic and population processes in jellyfish species will increase the ability to anticipate jellyfish outbreaks in the futureThe authors gratefully acknowledge financial support by the European Community Seventh Framework Programme (FP7/2007–2013) for the project VECTORS (grant agreement no. 266445) (URL: http://cordis.europa.eu/fp7/home_en.html). AC was supported by a doctoral fellowship from the Chilean National Commission for Scientific and Technological Research (CONICYT – PFCHA/Doctorado al Extranjero 4a Convocatoria, 72120016).Peer Reviewe

Overwintering of gelatinous zooplankton in the coastal Arctic Ocean.

Jellyfish and ctenophore blooms are of increasing concern for human enterprise in marine waters, although bloom development remains poorly understood. A key factor in popula- tion dynamics of blooms is individual lifespan, which for most gelatinous zooplankton is assumed to be only a few months, often from spring to autumn. Accumulating evidence, however, indicates that some species may overwinter. In this study, we used video photography to quantify medusae and ctenophores beneath land-fast sea ice in the Chukchi Sea near Utqiag ̇ vik (Barrow), Alaska, USA, from May to June, 2011−2014. Our data show large Chrysaora melanaster medusae over- wintering near the bottom, which could contribute to multiyear population increases suggested for this species. C. melanaster medusae were observed dragging their tentacles along the bottom, possibly feeding on epibenthic macrofauna. This highlights an underappreciated mechanism for benthic−pelagic coupling by gelatinous zooplankton. Additionally, numerous individuals of 3 ctenophore and 1 hydrozoan species were seen primarily at the sea−ice interface rather than near the bottom. We hypothesize that overwintering of medusae and ctenophores under sea ice is favored by the continued availability of prey, related to high productivity in the coastal Arctic, including production by ice algae. Successful overwintering may also relate to physical sheltering and low water temperatures. Overwintering may be a common life-history trait for gelatinous zoo- plankton, with important implications for understanding annual and interannual population trends in many environments and has important implications for models including jellyfish and ctenophore populations and ecosystem dynamics

High level of complexity and global diversity of the 3q29 locus revealed by optical mapping and long-read sequencing.

BACKGROUND: High sequence identity between segmental duplications (SDs) can facilitate copy number variants (CNVs) via non-allelic homologous recombination (NAHR). These CNVs are one of the fundamental causes of genomic disorders such as the 3q29 deletion syndrome (del3q29S). There are 21 protein-coding genes lost or gained as a result of such recurrent 1.6-Mbp deletions or duplications, respectively, in the 3q29 locus. While NAHR plays a role in CNV occurrence, the factors that increase the risk of NAHR at this particular locus are not well understood. METHODS: We employed an optical genome mapping technique to characterize the 3q29 locus in 161 unaffected individuals, 16 probands with del3q29S and their parents, and 2 probands with the 3q29 duplication syndrome (dup3q29S). Long-read sequencing-based haplotype resolved de novo assemblies from 44 unaffected individuals, and 1 trio was used for orthogonal validation of haplotypes and deletion breakpoints. RESULTS: In total, we discovered 34 haplotypes, of which 19 were novel haplotypes. Among these 19 novel haplotypes, 18 were detected in unaffected individuals, while 1 novel haplotype was detected on the parent-of-origin chromosome of a proband with the del3q29S. Phased assemblies from 44 unaffected individuals enabled the orthogonal validation of 20 haplotypes. In 89% (16/18) of the probands, breakpoints were confined to paralogous copies of a 20-kbp segment within the 3q29 SDs. In one del3q29S proband, the breakpoint was confined to a 374-bp region using long-read sequencing. Furthermore, we categorized del3q29S cases into three classes and dup3q29S cases into two classes based on breakpoints. Finally, we found no evidence of inversions in parent-of-origin chromosomes. CONCLUSIONS: We have generated the most comprehensive haplotype map for the 3q29 locus using unaffected individuals, probands with del3q29S or dup3q29S, and available parents, and also determined the deletion breakpoint to be within a 374-bp region in one proband with del3q29S. These results should provide a better understanding of the underlying genetic architecture that contributes to the etiology of del3q29S and dup3q29S

Questioning the rise of gelatinous zooplankton in the World's oceans

During the past several decades, high numbers of gelatinous zooplankton species have been reported in many estuarine and coastal ecosystems. Coupled with media-driven public perception, a paradigm has evolved in which the global ocean ecosystems are thought to be heading toward being dominated by “nuisance” jellyfish. We question this current paradigm by presenting a broad overview of gelatinous zooplankton in a historicalcontext to develop the hypothesis that population changes reflect the human-mediated alteration of global ocean ecosystems. To this end, we synthesize information related to the evolutionary context of contemporary gelatinous zooplankton blooms, the human frame of reference forchanges in gelatinous zooplankton populations, and whether sufficient data are available to have established the paradigm. We conclude that the current paradigm in which it is believed that there has been a global increase in gelatinous zooplankton is unsubstantiated, and we develop a strategy for addressing the critical questions about long-term, human-related changes in the sea as they relate to gelatinous zooplankton blooms

Cis-Acting Regulation of Brain-Specific ANK3 Gene Expression by a Genetic Variant Associated with Bipolar Disorder

Several genome-wide association studies (GWAS) for bipolar disorder (BD) have found a strong association of the Ankyrin3 (ANK3) gene. This association spans numerous linked single nucleotide polymorphisms (SNPs) in a ~250 kb genomic region overlapping ANK3. The associated region encompasses predicted regulatory elements as well as two of six validated alternative first exons, which encode distinct protein domains at the N-terminus of the protein also known as ankyrin-G (AnkG). Using RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM-RACE) to identify novel transcripts in conjunction with a highly sensitive, exon-specific multiplexed mRNA expression assay, we detected differential regulation of distinct ANK3 transcription start sites (TSSs) and coupling of specific 5’ ends with 3’ mRNA splicing events in post-mortem human brain and human stem cell-derived neural progenitors and neurons. Furthermore, allelic variation at the BD–associated SNP rs1938526 correlated with a significant difference in cerebellar expression of a brain-specific ANK3 transcript. These findings suggest a brain-specific cis-regulatory transcriptional effect of ANK3 may be relevant to BD pathophysiology

Clonal Hematopoiesis and Blood-Cancer Risk Inferred from Blood DNA Sequence

Background Cancers arise from multiple acquired mutations, which presumably occur over many years. Early stages in cancer development might be present years before cancers become clinically apparent. Methods We analyzed data from whole-exome sequencing of DNA in peripheral-blood cells from 12,380 persons, unselected for cancer or hematologic phenotypes. We identified somatic mutations on the basis of unusual allelic fractions. We used data from Swedish national patient registers to follow health outcomes for 2 to 7 years after DNA sampling. Results Clonal hematopoiesis with somatic mutations was observed in 10% of persons older than 65 years of age but in only 1% of those younger than 50 years of age. Detectable clonal expansions most frequently involved somatic mutations in three genes (DNMT3A, ASXL1, and TET2) that have previously been implicated in hematologic cancers. Clonal hematopoiesis was a strong risk factor for subsequent hematologic cancer (hazard ratio, 12.9; 95% confidence interval, 5.8 to 28.7). Approximately 42% of hematologic cancers in this cohort arose in persons who had clonality at the time of DNA sampling, more than 6 months before a first diagnosis of cancer. Analysis of bone marrow–biopsy specimens obtained from two patients at the time of diagnosis of acute myeloid leukemia revealed that their cancers arose from the earlier clones. Conclusions Clonal hematopoiesis with somatic mutations is readily detected by means of DNA sequencing, is increasingly common as people age, and is associated with increased risks of hematologic cancer and death. A subset of the genes that are mutated in patients with myeloid cancers is frequently mutated in apparently healthy persons; these mutations may represent characteristic early events in the development of hematologic cancers. (Funded by the National Human Genome Research Institute and others.)National Human Genome Research Institute (U.S.) (Grant U54 HG003067)National Human Genome Research Institute (U.S.) (Grant R01 HG006855)Stanley Center for Psychiatric ResearchAlexander and Margaret Stewart TrustNational Institute of Mental Health (U.S.) (Grant R01 MH 077139)National Institute of Mental Health (U.S.) (Grant RC2 MH089905)Sylvan C. Herman Foundatio

Ears of the Armadillo: Global Health Research and Neglected Diseases in Texas

Neglected tropical diseases (NTDs) have\ud been recently identified as significant public\ud health problems in Texas and elsewhere in\ud the American South. A one-day forum on the\ud landscape of research and development and\ud the hidden burden of NTDs in Texas\ud explored the next steps to coordinate advocacy,\ud public health, and research into a\ud cogent health policy framework for the\ud American NTDs. It also highlighted how\ud U.S.-funded global health research can serve\ud to combat these health disparities in the\ud United States, in addition to benefiting\ud communities abroad