TaMSH7: A cereal mismatch repair gene that affects fertility in transgenic barley (Hordeum vulgare L.)

Background: Chromosome pairing, recombination and DNA repair are essential processes during meiosis in sexually reproducing organisms. Investigating the bread wheat (Triticum aestivum L.) Ph2 (Pairing homoeologous) locus has identified numerous candidate genes that may have a role in controlling such processes, including TaMSH7, a plant specific member of the DNA mismatch repair family. Results: Sequencing of the three MSH7 genes, located on the short arms of wheat chromosomes 3A, 3B and 3D, has revealed no significant sequence divergence at the amino acid level suggesting conservation of function across the homoeogroups. Functional analysis of MSH7 through the use of RNAi loss-of-function transgenics was undertaken in diploid barley (Hordeum vulgare L.). Quantitative real-time PCR revealed several T0 lines with reduced MSH7 expression. Positive segregants from two T1 lines studied in detail showed reduced MSH7 expression when compared to transformed controls and null segregants. Expression of MSH6, another member of the mismatch repair family which is most closely related to the MSH7 gene, was not significantly reduced in these lines. In both T1 lines, reduced seed set in positive segregants was observed. Conclusion: Results presented here indicate, for the first time, a distinct functional role for MSH7 in vivo and show that expression of this gene is necessary for wild-type levels of fertility. These observations suggest that MSH7 has an important function during meiosis and as such remains a candidate for Ph2.Andrew H Lloyd, Andrew S Milligan, Peter Langridge, and Jason A Abl

Properties of Sequential Chromospheric Brightenings and Associated Flare Ribbons

We report on the physical properties of solar sequential chromospheric brightenings (SCBs) observed in conjunction with moderate-sized chromospheric flares with associated CMEs. To characterize these ephemeral events, we developed automated procedures to identify and track subsections (kernels) of solar flares and associated SCBs using high resolution H-alpha images. Following the algorithmic identification and a statistical analysis, we compare and find the following: SCBs are distinctly different from flare kernels in their temporal characteristics of intensity, Doppler structure, duration, and location properties. We demonstrate that flare ribbons are themselves made up of subsections exhibiting differing characteristics. Flare kernels are measured to have a mean propagation speed of 0.2 km/s and a maximum speed of 2.3 km/s over a mean distance of 5 x 10^3 km. Within the studied population of SCBs, different classes of characteristics are observed with coincident negative, positive, or both negative and positive Doppler shifts of a few km/s. The appearance of SCBs precede peak flare intensity by ~12 minutes and decay ~1 hour later. They are also found to propagate laterally away from flare center in clusters at 41 km/s or 89 km/s. Given SCBs distinctive nature compared to flares, we suggest a different physical mechanism relating to their origin than the associated flare. We present a heuristic model of the origin of SCBs.Comment: 24 pages, 17 figure

An Improved Model for Dynamin Assembly Revealed by Cryo-EM

Continua com: Avaluació de la qualitat de l'aire a la ciutat de Barcelon

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Thermodynamics and Kinetics of Adaptive Binding in the Malachite Green RNA Aptamer

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biochemistry, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see http://dx.doi.org/10.1021/bi400549sAdaptive binding, the ability of molecules to fold themselves around the structure of a ligand and thereby incorporating it into their three-dimensional fold, is a key feature of most RNA aptamers. The malachite green aptamer (MGA) has been shown to bind several closely related triphenyl dyes with planar and nonplanar structures in this manner. Competitive binding studies using isothermal titration calorimetry and stopped flow kinetics have been conducted with the aim of understanding the adaptive nature of RNA–ligand interaction. The results of these studies reveal that binding of one ligand can reduce the ability of the aptamer pocket to adapt to another ligand, even if this second ligand has a significantly higher affinity to the free aptamer. A similar effect is observed in the presence of Mg2+ ions which stabilize the binding pocket in a more ligand bound-like conformation.National Science and Engineering Research Council (NSERC) [326911-2009]Canada Foundation for Innovation (CFI

The impact of immediate breast reconstruction on the time to delivery of adjuvant therapy: the iBRA-2 study

Background: Immediate breast reconstruction (IBR) is routinely offered to improve quality-of-life for women requiring mastectomy, but there are concerns that more complex surgery may delay adjuvant oncological treatments and compromise long-term outcomes. High-quality evidence is lacking. The iBRA-2 study aimed to investigate the impact of IBR on time to adjuvant therapy. Methods: Consecutive women undergoing mastectomy ± IBR for breast cancer July–December, 2016 were included. Patient demographics, operative, oncological and complication data were collected. Time from last definitive cancer surgery to first adjuvant treatment for patients undergoing mastectomy ± IBR were compared and risk factors associated with delays explored. Results: A total of 2540 patients were recruited from 76 centres; 1008 (39.7%) underwent IBR (implant-only [n = 675, 26.6%]; pedicled flaps [n = 105,4.1%] and free-flaps [n = 228, 8.9%]). Complications requiring re-admission or re-operation were significantly more common in patients undergoing IBR than those receiving mastectomy. Adjuvant chemotherapy or radiotherapy was required by 1235 (48.6%) patients. No clinically significant differences were seen in time to adjuvant therapy between patient groups but major complications irrespective of surgery received were significantly associated with treatment delays. Conclusions: IBR does not result in clinically significant delays to adjuvant therapy, but post-operative complications are associated with treatment delays. Strategies to minimise complications, including careful patient selection, are required to improve outcomes for patients

A cereal mismatch repair gene that affects fertility in transgenic barley (L.)-0

<p><b>Copyright information:</b></p><p>Taken from ": A cereal mismatch repair gene that affects fertility in transgenic barley (L.)"</p><p>http://www.biomedcentral.com/1471-2229/7/67</p><p>BMC Plant Biology 2007;7():67-67.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2234410.</p><p></p>cture when transcribed. This dsRNA may then reduce expression through RNAi. The construct contains a hygromycin resistance gene, hygromycin phosphotransferase (), which was used as a selectable marker during tissue culture. This gene was also utilised for analysis of transgene segregation in the Tpopulation

A cereal mismatch repair gene that affects fertility in transgenic barley (L.)-1

<p><b>Copyright information:</b></p><p>Taken from ": A cereal mismatch repair gene that affects fertility in transgenic barley (L.)"</p><p>http://www.biomedcentral.com/1471-2229/7/67</p><p>BMC Plant Biology 2007;7():67-67.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2234410.</p><p></p> empty vector control, lane 9 – non-transformed barley control, lanes 10 to 15 – various transgenic lines (#, , , , , ), lane 16 – transformed empty vector control, lanes 17 and 18 – transgenic lines and respectively. Copy numbers for selected lines represented on this blot (-, , , , , ) and subsequently analysed by Q-PCR for expression levels, are highlighted in Table 1