Evaluation of IPM modules for the management of fruit borer and fruit rot diseases in tomato, Lycopersicon esculentum Miller

Among, five IPM modules tested against tomato fruit borer and fruit rot on tomato, the IPM module (M3) consisting of use of pheromone traps (@ 12 traps/ha) just after transplanting the tomato crop , Lycopersicon esculentum Miller for monitoring the population of Helicoverpa armigera . followed by three foliar sprays commencing with a mixture of lamba-cyhalothrin 5EC @ 0.8ml/L(0.04%) and Dithane Z-78 (Zineb) @ 2.5g/L (0.25%) after 10 days of appearance of moths in the traps (after 30 days of transplanting) followed by spray with a mixture of Helicide (Ha NPV) 100 LE @ 0.5ml/L+ Indofil M-45 @ 2.5g/L (0.25%) + Gur (0.05%) + Tween 80 (0.05%) after 15 days of first spray followed by spray with a mixture of lamba-cyhalothrin 5EC @ 0.8ml/L(0.04%) and moximate (cymoxanil + mancozeb) @ 0.25% after 15 days of the second spray was found to be most effective in minimizing the infestation of fruit borer and fruit rot diseases with 50.00% and 63.45% reduction over control, respectively. This module was also found to be most economic resulting in highest marketable fruit yield (255.94q/ha) and maximum net returns (Rs.10.36) per rupee spent. The present findings are of immense utility as there will be reduction in number of sprays resulting in the cost of production of tomato crop

An empirical investigation on the deployment of Operational excellence in SMEs

Purpose: This study investigates the quantitative aspect of the various strains of Operational excellence (OE) and “Competitive-potential (CP)” in the SME sector. It has five steps, i.e., identifying the key performance constructs of OE and their hypothesized relationship pattern from literature, validating these constructs through factor analysis, formalizing their empirical relationships by structural-equation-modeling (SEM), path analysis of performance constructs with the empirical results, and lastly proposing a framework for OE deployment in SMEs. Design/methodology/approach: Data for the deployment scores of Operational excellence procedures (OEPs) were collected through a structured questionnaire survey. Nine hundred participants from a stratified random sample were approached for the survey, and 473 responses were received. Sample stratification was based on Gender, Education, Experience, Position, Department, and Industry. Respondents had 5 to 30 years of experience managing manufacturing operations, holding the Manager position and above. Findings: The path analysis of the structural model provides unique insights into OE’s practical aspects in SMEs (Small and Medium Enterprises). Such as Contractual-conformance and Process-efficiency play pivotal roles as both have a significant positive impact on CP. Supplier efficacy, Consistency, and Product-excellence do not improve CP unless mediated by Contractual-conformance or Process-efficiency. Research limitations/implications: The study provides important implications for academia, policymakers, and managers. The study identifies and validates the operational excellence key performance practices and proposes a framework for manufacturing organizations. SME managers can follow the framework to develop effective operational excellence strategies to help them achieve their organizational goals. Additionally, the study emphasizes the need for continuous culture in SMEs, which will help to support operational excellence deployment. Overall, the implications presented in the study will help SMEs to enhance their competitiveness and operational performance. Originality/value: The study explores the empirical investigation of the operational excellence deployment in SMEs. The study uses a mixed method approach for research design, including qualitative and quantitative approaches, and uses SEM to test the proposed framework. Validation of OE’s six key performance constructs and establishing their empirical relation is an attempt to advance the Operations excellence theory. Unlike large enterprises, SMEs demonstrate an incohesive response to the practices pertaining to Supplier efficacy, Consistency, and Product-excellence. This unique response pattern requires special treatment, which is incorporated into the proposed framework

Machine Learning Applications for Sustainable Manufacturing: A Bibliometric-based Review for Future Research

The role of data analytics is significantly important in manufacturing industries as it holds the key to address sustainability challenges and handle the large amount of data generated from different types of manufacturing operations. The present study, therefore, aims to conduct a systematic and bibliometric-based review in the applications of machine learning (ML) techniques for sustainable manufacturing (SM). In the present study, we use a bibliometric review approach that is focused on the statistical analysis of published scientific documents with an unbiased objective of the current status and future research potential of ML applications in sustainable manufacturing. The present study highlights how manufacturing industries can benefit from ML techniques when applied to address SM issues. Based on the findings, a ML-SM framework is proposed. The framework will be helpful to researchers, policymakers and practitioners to provide guidelines on the successful management of SM practices. A comprehensive and bibliometric review of opportunities for ML techniques in SM with a framework is still limited in the available literature. This study addresses the bibliometric analysis of ML applications in SM, which further adds to the originalityN/

ZBTB32 restrains antibody responses to murine cytomegalovirus infections, but not other repetitive challenges

ZBTB32 is a transcription factor that is highly expressed by a subset of memory B cells and restrains the magnitude and duration of recall responses against hapten-protein conjugates. To define physiological contexts in which ZBTB32 acts, we assessed responses by Zbtb32-/- mice or bone marrow chimeras against a panel of chronic and acute challenges. Mixed bone marrow chimeras were established in which all B cells were derived from either Zbtb32-/- mice or control littermates. Chronic infection of Zbtb32-/- chimeras with murine cytomegalovirus led to nearly 20-fold higher antigen-specific IgG2b levels relative to controls by week 9 post-infection, despite similar viral loads. In contrast, IgA responses and specificities in the intestine, where memory B cells are repeatedly stimulated by commensal bacteria, were similar between Zbtb32-/- mice and control littermates. Finally, an infection and heterologous booster vaccination model revealed no role for ZBTB32 in restraining primary or recall antibody responses against influenza viruses. Thus, ZBTB32 does not limit recall responses to a number of physiological acute challenges, but does restrict antibody levels during chronic viral infections that periodically engage memory B cells. This restriction might selectively prevent recall responses against chronic infections from progressively overwhelming other antibody specificities.National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01AI99109, R01AI131680, U01AI131349, K08AI04991]; New York Stem Cell FoundationOpen access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Exploiting genetic diversity of walnut (Juglans regia) from Jammu region of India for export related traits

The present field work was done to study the existing genetic diversity and its exploitation through identifying elite walnut (Juglans regia L.) mother trees from the large number of naturally growing seedling trees in erstwhile Doda district of Jammu region of Jammu & Kashmir state. The field work was done from 2006 to 2012. A total of 63 walnut trees were marked as elite, out of 790 trees visited at 18 locations situated at 1500m-1750m above mean sea level. Out of total collections, 49.20% samples have attractive colour influencing the consumer acceptability. Five collections (BS1207, BT1807, MR0309, MR0509 and GL0109) had kernel recovery of >50%. Wide range of variability was recorded for various nut characters being highest in kernel recovery (18.54-61.40%) with highest coefficient of variation. Nut weight was positively correlated with nut size. The total variance in first principal component was due to nut size and weight while, in second component, it was contributed by kernel recovery. Further, analysis based on export quality traits (prescribed by J&K walnut grower association) lead to the identification of 8 most promising walnut clones, viz. BS1207, PK2707, MR0309, MR0509, MR0809, GL0109, PR0309 and NZ0112. Of these, GL0109 proved best in respect of export related traits and can be developed as a good table cultivar

Rapid Downregulation of DAB2 by Toll-Like Receptor Activation Contributes to a Pro-Inflammatory Switch in Activated Dendritic Cells

Dendritic cells (DCs) are pivotal in regulating tolerogenic as well as immunogenic responses against microorganisms by directing both the innate and adaptive immune response. In health, phenotypically different DC subsets found in the gut mucosa are maintained in their tolerogenic state but switch to a pro-inflammatory phenotype during infection or chronic autoinflammatory conditions such as inflammatory bowel disease (IBD). The mechanisms that promote the switch among the mucosal DCs from a tolerogenic to an immunogenic, pro-inflammatory phenotype are incompletely understood. We hypothesized that disabled homolog 2 (DAB2), recently described as a negative regulator of DC immunogenicity during their development, is regulated during intestinal inflammation and modulates mucosal DC function. We show that DAB2 is highly expressed in colonic CD11b+CD103− DCs, a subset known for its capacity to induce inflammatory Th1/Th17 responses in the colon, and is downregulated predominantly in this DC subset during adoptive T cell transfer colitis. Administration of Dab2-deficient DCs (DC2.4Dab2−/− cells) modulated the course of DSS colitis in wild-type mice, enhanced mucosal expression of Tnfa, Il6, and Il17a, and promoted neutrophil recruitment. In bone-marrow derived dendritic cells (BMDC), DAB2 expression correlated with CD11b levels and DAB2 was rapidly and profoundly inhibited by TLR ligands in a TRIF- and MyD88-dependent manner. The negative modulation of DAB2 was biphasic, initiated with a quick drop in DAB2 protein, followed by a sustained reduction in Dab2 mRNA. DAB2 downregulation promoted a more functional and activated DC phenotype, reduced phagocytosis, and increased CD40 expression after TLR activation. Furthermore, Dab2 knockout in DCs inhibited autophagy and promoted apoptotic cell death. Collectively, our results highlight the immunoregulatory role for DAB2 in the intestinal dendritic cells and suggest that DAB2 downregulation after microbial exposure promotes their switch to an inflammatory phenotype

Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya

The importance of medicinal plants in traditional healthcare practices, providing clues to new areas of research and in biodiversity conservation is now well recognized. However, information on the uses for plants for medicine is lacking from many interior areas of Himalaya. Keeping this in view the present study was initiated in a tribal dominated hinterland of western Himalaya. The study aimed to look into the diversity of plant resources that are used by local people for curing various ailments. Questionnaire surveys, participatory observations and field visits were planned to illicit information on the uses of various plants. It was found that 35 plant species are commonly used by local people for curing various diseases. In most of the cases (45%) under ground part of the plant was used. New medicinal uses of Ranunculus hirtellus and Anemone rupicola are reported from this area. Similarly, preparation of "sik" a traditional recipe served as a nutritious diet to pregnant women is also not documented elsewhere. Implication of developmental activities and changing socio-economic conditions on the traditional knowledge are also discussed

Clinical isolates of the modern Mycobacterium tuberculosis lineage 4 evade host defense in human macrophages through eluding IL-1\u3b2-induced autophagy article

Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), has infected over 1.7 billion people worldwide and causes 1.4 million deaths annually. Recently, genome sequence analysis has allowed the reconstruction of Mycobacterium tuberculosis complex (MTBC) evolution, with the identification of seven phylogeographic lineages: four referred to as evolutionarily "ancient", and three "modern". The MTBC strains belonging to "modern" lineages appear to show enhanced virulence that may have warranted improved transmission in humans over ancient lineages through molecular mechanisms that remain to be fully characterized. To evaluate the impact of MTBC genetic diversity on the innate immune response, we analyzed intracellular bacterial replication, inflammatory cytokine levels, and autophagy response in human primary macrophages infected with MTBC clinical isolates belonging to the ancient lineages 1 and 5, and the modern lineage 4. We show that, when compared to ancient lineage 1 and 5, MTBC strains belonging to modern lineage 4 show a higher rate of replication, associated to a significant production of proinflammatory cytokines (IL-1\u3b2, IL-6, and TNF-\u3b1) and induction of a functional autophagy process. Interestingly, we found that the increased autophagic flux observed in macrophages infected with modern MTBC is due to an autocrine activity of the proinflammatory cytokine IL-1\u3b2, since autophagosome maturation is blocked by an interleukin-1 receptor antagonist. Unexpectedly, IL-1\u3b2-induced autophagy is not disadvantageous for the survival of modern Mtb strains, which reside within Rab5-positive phagosomal vesicles and avoid autophagosome engulfment. Altogether, these results suggest that autophagy triggered by inflammatory cytokines is compatible with a high rate of intracellular bacilli replication and may therefore contribute to the increased pathogenicity of the modern MTBC lineages

Human Antibodies that Slow Erythrocyte Invasion Potentiate Malaria-Neutralizing Antibodies.

The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is the leading target for next-generation vaccines against the disease-causing blood-stage of malaria. However, little is known about how human antibodies confer functional immunity against this antigen. We isolated a panel of human monoclonal antibodies (mAbs) against PfRH5 from peripheral blood B cells from vaccinees in the first clinical trial of a PfRH5-based vaccine. We identified a subset of mAbs with neutralizing activity that bind to three distinct sites and another subset of mAbs that are non-functional, or even antagonistic to neutralizing antibodies. We also identify the epitope of a novel group of non-neutralizing antibodies that significantly reduce the speed of red blood cell invasion by the merozoite, thereby potentiating the effect of all neutralizing PfRH5 antibodies as well as synergizing with antibodies targeting other malaria invasion proteins. Our results provide a roadmap for structure-guided vaccine development to maximize antibody efficacy against blood-stage malaria. Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved