Join the Band: Benefits of Engaging Intergenerational Volunteers in the Local Church Worship Ministry

Throughout the Bible and much of church history, intergenerational ministry was the common practice. However, in recent decades many local evangelical churches have abandoned the practice of intergenerational ministry and have instead embraced age-segregated ministry models. Reflecting this shift, age-segregated worship ministries within the local evangelical church have become the norm. This qualitative study examined biblical examples and teachings regarding worship and ministry and examined worship and ministry practices from throughout church history. Analysis of the research findings addressed the characteristics of an effective intergenerational worship ministry. Although facilitating an intergenerational worship ministry is a complicated endeavor, this study revealed that participation in an effective worship ministry is relationally, developmentally, musically, and spiritually beneficial for all involved. While it is true that each generational cohort benefits from involvement in intergenerational worship ministry, research indicates that such participation is especially beneficial for the youngest generations. Therefore, this project also examined the generational characteristics and needs of Gen Z (those born between 1995 and 2010) in order to ascertain how to best engage them as active participants in intergenerational worship ministries within the local evangelical church

Design and optimisation of expression vectors for the synthesis of eukaryotic proteins in plants

Recombinant proteins are vital in sectors like medicine, industry, and agriculture. Conventional expression systems face challenges such as the inability of prokaryotic systems to perform eukaryotic post-translational modifications and the high costs of scaling up eukaryotic cell cultures. Plants are scalable and can produce eukaryotic modifications but suffer from low yields and underdeveloped downstream processing methods. This thesis aimed to enhance plant-based production of recombinant proteins, focussing on developing and optimizing plant expression vectors and protein processing procedures to maximize yields. A range of eukaryotic proteins, including those from plants and mammals, varying in size and sub-cellular localisation were expressed to understand how protein complexity affects yield. The goal was to express full-length rat and human P2X7 receptors and two truncated versions of isolated cytoplasmic domains. Using Golden Gate and conventional cloning, several expression constructs were created. These constructs significantly increased eGFP production in plants, up to 199-fold more than initial constructs and 2.2-fold more than the previously published pJL-TRBO vector. By comparing different protein extraction and purification methods an effective downstream processing pipeline was developed, enabling eGFP quantification of up to 20% total soluble protein. Compared to starting constructs, eGFP production was increased 254-fold at the protein level using the best construct when measured by western blot. With methods optimised for eGFP, other target proteins were expressed, extracted, purified, and quantified. This led to the development of several useful expression constructs for plants, enabling detectable production of the 53 kDa isolated cytoplasmic domain of P2X7 in a transient N. benthamiana expression system, although full-length transmembrane protein expression was not detected. Overall, this research successfully developed optimized expression vectors and protein extraction and purification techniques for high yields of poly(His)-tagged cytoplasmic proteins and paves the way for future large-scale research to express more diverse protein targets

Diamagnetic depression observations at Saturn’s magnetospheric cusp by the Cassini spacecraft

The magnetospheric cusp is a region where shocked solar wind plasma can enter a planetary magnetosphere, after magnetic reconnection has occurred at the dayside magnetopause or in the lobes. The dense plasma that enters the high‐latitude magnetosphere creates diamagnetic effects whereby a depression is observed in the magnetic field. We present observations of the cusp events at Saturn’s magnetosphere where these diamagnetic depressions are found. The data are subtracted from a magnetic field model, and the calculated magnetic pressure deficits are compared to the particle pressures. A high plasma pressure layer in the magnetosphere adjacent to the cusp is discovered to also depress the magnetic field, outside of the cusp. This layer is observed to contain energetic He++ (up to ∼100 keV) from the solar wind as well as heavy water group ions (W+) originating from the moon Enceladus. We also find a modest correlation of diamagnetic depression strength to solar wind dynamic pressure and velocity; however, unlike at Earth, there is no correlation found with He++ counts.Key PointsDiamagnetic depressions are found in the cusp and are observed to continue into the adjacent magnetosphereA heated plasma layer of mixed composition is found to depress the adjacent magnetospheric fieldDiamagnetic depression strength is correlated to solar wind dynamic pressure and velocity but not to the observed He++ counts, like at EarthPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137687/1/jgra53517_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137687/2/jgra53517-sup-0001-supinfo.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137687/3/jgra53517.pd

Left Ventricular Structure and Function in Elite Swimmers and Runners

Sport-specific differences in the left ventricle (LV) of land-based athletes have been observed; however, comparisons to water-based athletes are sparse. The purpose of this study was to examine differences in LV structure and function in elite swimmers and runners. Sixteen elite swimmers [23 (2) years, 81% male, 69% white] and 16 age, sex, and race matched elite runners participated in the study. All athletes underwent resting echocardiography and indices of LV dimension, global LV systolic and diastolic function, and LV mechanics were determined. All results are presented as swimmers vs. runners. Early diastolic function was lower in swimmers including peak early transmitral filling velocity [76 (13) vs. 87 (11) cm ⋅ s-1, p = 0.02], mean mitral annular peak early velocity [16 (2) vs. 18 (2) cm ⋅ s-1, p = 0.01], and the ratio of peak early to late transmitral filling velocity [2.68 (0.59) vs. 3.29 (0.72), p = 0.005]. The diastolic mechanics index of time to peak untwisting rate also occurred later in diastole in swimmers [12 (10)% diastole vs. 5 (4)% diastole, p = 0.01]. Cardiac output was larger in swimmers [5.8 (1.5) vs. 4.7 (1.2) L ⋅ min-1, p = 0.04], which was attributed to their higher heart rates [56 (6) vs. 49 (6) bpm, p < 0.001] given stroke volumes were similar between groups. All other indices of LV systolic function and dimensions were similar between groups. Our findings suggest enhanced early diastolic function in elite runners relative to swimmers, which may be attributed to faster LV untwisting

The cruel optimism of mobility : aspiration, belonging, and the “good life” among transnational Chinese migrants in Tokyo

Over the past thirty years, moving overseas has been a positively valued aspiration in China. On both a government level, and within popular discourse, migration has been propagated as a means to be better citizens, and a better nation, resonating with families’ desire for a better life. However, there are consequences for those who move, in terms of belonging and how they imagine their life projects. This article extends the established scholarship on mobility out of China by comparing the rhetorical construction of mobility with the experiences of Chinese migrants in Japan. Based on ethnographic fieldwork among educationally channeled Chinese migrants in Tokyo, I show how the imaginaries that shape migrant projects are constituted by conflicting aspirations and desires. The mismatch between daily experiences and discursively informed perceptions of what constitutes a “good life” and “success,” in many senses resemble what Lauren Berlant has called “cruel optimism.” Educationally channeled migration out of China is posited as a desirable object-idea that is “cruel” because the “cluster of promises” that constitute its “optimism” cannot be reconciled with the mobile lifeworlds of many Chinese transnational migrants. Due to the impossibility of simultaneously achieving the promises of success, pleasing one’s family, and attaining a sense of cosmopolitanism, many migrants resign themselves to the instabilities of mobile life. Their experiences are suggestive of the consequences of a world that increasingly celebrates mobility, with implications for how “being at home in the world” is imagined today

Saturn's open‐closed field line boundary:a Cassini electron survey at Saturn's magnetosphere

We investigate the average configuration and structure of Saturn's magnetosphere in the nightside equatorial and high‐latitude regions. Electron data from the Cassini Plasma Spectrometer's Electron Spectrometer (CAPS‐ELS) is processed to produce a signal‐to‐noise ratio for the entire CAPS‐ELS time of operation at Saturn's magnetosphere. We investigate where the signal‐to‐noise ratio falls below 1, to identify regions in the magnetosphere where there is a significant depletion in the electron content. In the nightside equatorial region we use this to find that the most planetward reconnection x‐line location is at 20 – 25 RS downtail from the planet in the midnight to dawn sector. We also find an equatorial dawn‐dusk asymmetry at a radial distance of >20 RS which may indicate the presence of plasma depleted flux tubes returning to the dayside after reconnection in the tail. Furthermore, we find that the high‐latitude magnetosphere is predominantly in a state of constant plasma depletion and located on open field lines. We map the region of high‐latitude magnetosphere that is depleted of electrons to the polar cap to estimate the size and open flux content within the polar caps. The mean open flux content for the northern and southern polar caps are found to be 25±5 and 32±5 GWb, respectively. The average location of the open‐closed field boundary is found at invariant colatitudes of 12.7±0.6° and 14.5±0.6°. The northern boundary is modulated by planetary period oscillations more than the southern boundary

Dominant protection from HLA-linked autoimmunity by antigen-specific regulatory T cells

Susceptibility and protection against human autoimmune diseases, including type I diabetes, multiple sclerosis, and Goodpasture disease, is associated with particular human leukocyte antigen (HLA) alleles. However, the mechanisms underpinning such HLA-mediated effects on self-tolerance remain unclear. Here we investigate the molecular mechanism of Goodpasture disease, an HLA-linked autoimmune renal disorder characterized by an immunodominant CD4+ T-cell self-epitope derived from the α3 chain of type IV collagen (α3135–145)1,2,3,4. While HLA-DR15 confers a markedly increased disease risk, the protective HLA-DR1 allele is dominantly protective in trans with HLA-DR15 (ref. 2). We show that autoreactive α3135–145-specific T cells expand in patients with Goodpasture disease and, in α3135–145-immunized HLA-DR15 transgenic mice, α3135–145-specific T cells infiltrate the kidney and mice develop Goodpasture disease. HLA-DR15 and HLA-DR1 exhibit distinct peptide repertoires and binding preferences and present the α3135–145 epitope in different binding registers. HLA-DR15-α3135–145 tetramer+ T cells in HLA-DR15 transgenic mice exhibit a conventional T-cell phenotype (Tconv) that secretes pro-inflammatory cytokines. In contrast, HLA-DR1-α3135–145 tetramer+ T cells in HLA-DR1 and HLA-DR15/DR1 transgenic mice are predominantly CD4+Foxp3+ regulatory T cells (Treg cells) expressing tolerogenic cytokines. HLA-DR1-induced Treg cells confer resistance to disease in HLA-DR15/DR1 transgenic mice. HLA-DR15+ and HLA-DR1+ healthy human donors display altered α3135–145-specific T-cell antigen receptor usage, HLA-DR15-α3135–145 tetramer+ Foxp3− Tconv and HLA-DR1-α3135–145 tetramer+ Foxp3+CD25hiCD127lo Treg dominant phenotypes. Moreover, patients with Goodpasture disease display a clonally expanded α3135–145-specific CD4+ T-cell repertoire. Accordingly, we provide a mechanistic basis for the dominantly protective effect of HLA in autoimmune disease, whereby HLA polymorphism shapes the relative abundance of self-epitope specific Treg cells that leads to protection or causation of autoimmunity

Propulsion in cubomedusae : mechanisms and utility

© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 8 (2013): e56393, doi:10.1371/journal.pone.0056393.Evolutionary constraints which limit the forces produced during bell contractions of medusae affect the overall medusan morphospace such that jet propulsion is limited to only small medusae. Cubomedusae, which often possess large prolate bells and are thought to swim via jet propulsion, appear to violate the theoretical constraints which determine the medusan morphospace. To examine propulsion by cubomedusae, we quantified size related changes in wake dynamics, bell shape, swimming and turning kinematics of two species of cubomedusae, Chironex fleckeri and Chiropsella bronzie. During growth, these cubomedusae transitioned from using jet propulsion at smaller sizes to a rowing-jetting hybrid mode of propulsion at larger sizes. Simple modifications in the flexibility and kinematics of their velarium appeared to be sufficient to alter their propulsive mode. Turning occurs during both bell contraction and expansion and is achieved by generating asymmetric vortex structures during both stages of the swimming cycle. Swimming characteristics were considered in conjunction with the unique foraging strategy used by cubomedusae.This work was supported by an ONR MURI award (N000140810654) and National Science Foundation grant OCE 0623508 to JHC, SPC, JOD. And the work was supported by the Roger Williams University Foundation to Promote Scholarship

Lets Talk about Race: Identity, Chatbots, and AI

Why is it so hard for chatbots to talk about race? This work explores how the biased contents of databases, the syntactic focus of natural language processing, and the opaque nature of deep learning algorithms cause chatbots difficulty in handling race-talk. In each of these areas, the tensions between race and chatbots create new opportunities for people and machines. By making the abstract and disparate qualities of this problem space tangible, we can develop chatbots that are more capable of handling race-talk in its many forms. Our goal is to provide the HCI community with ways to begin addressing the question, how can chatbots handle race-talk in new and improved ways