BOSS Ultracool Dwarfs I: Colors and Magnetic Activity of M and L dwarfs

We present the colors and activity of ultracool (M7-L8) dwarfs from the Tenth Data Release of the Sloan Digital Sky Survey (SDSS). We combine previous samples of SDSS M and L dwarfs with new data obtained from the Baryon Oscillation Sky Survey (BOSS) to produce the BOSS Ultracool Dwarf (BUD) sample of 11820 M7-L8 dwarfs. By combining SDSS data with photometry from the Two Micron All Sky Survey and the Wide-Field Infrared Sky Explorer mission, we present ultracool dwarf colors from $i-z$ to $W2-W3$ as a function of spectral type, and extend the SDSS-2MASS-WISE color locus to include ultracool dwarfs. The $i-z$, $i-J$, and $z-J$ colors provide the best indication of spectral type for M7-L3 dwarfs. We also examine ultracool dwarf chromospheric activity through the presence and strength of H$\alpha$ emission. The fraction of active dwarfs rises through the M spectral sequence until it reaches $\sim$90% at spectral type L0. The fraction of active dwarfs then declines to 50% at spectral type L5; no H$\alpha$ emission is observed in the late-L dwarfs in the BUD sample. The fraction of active L0-L5 dwarfs is much higher than previously observed. The strength of activity declines with spectral type from M7 through L3, after which the data do not show a clear trend. Using one-dimensional chromosphere models, we explore the range of filling factors and chromospheric temperature structures that are consistent with H$\alpha$ observations of M0-L7 dwarfs. M dwarf chromospheres have a similar, smoothly varying range of temperature and surface coverage while L dwarf chromospheres are cooler and have smaller filling factors.Comment: 24 pages and 13 figures, submitted to AJ. A short video describing these results can be found at https://www.youtube.com/watch?v=wwX5WkuJCU

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.

Heart of Glass (HEG1), a transmembrane receptor, and Rasip1, an endothelial-specific Rap1-binding protein, are both essential for cardiovascular development. Here we performed a proteomic screen for novel HEG1 interactors and report that HEG1 binds directly to Rasip1. Rasip1 localizes to forming endothelial cell (EC) cell-cell junctions and silencing HEG1 prevents this localization. Conversely, mitochondria-targeted HEG1 relocalizes Rasip1 to mitochondria in cells. The Rasip1-binding site in HEG1 contains a 9 residue sequence, deletion of which abrogates HEG1's ability to recruit Rasip1. HEG1 binds to a central region of Rasip1 and deletion of this domain eliminates Rasip1's ability to bind HEG1, to translocate to EC junctions, to inhibit ROCK activity, and to maintain EC junctional integrity. These studies establish that the binding of HEG1 to Rasip1 mediates Rap1-dependent recruitment of Rasip1 to and stabilization of EC cell-cell junctions

Application of Aura OMI L2G Products Compared with NASA MERRA-2 Assimilation

The Ozone Monitoring Instrument (OMI) is one of the instruments aboard NASA's Aura satellite. It measures ozone total column and vertical profile, aerosols, clouds, and trace gases including NO2, SO2, HCHO, BrO, and OClO using absorption in the ultraviolet electromagnetic spectrum (280 - 400 nm). OMI Level-2G (L2G) products are based on the pixel-level OMI granule satellite measurements stored within global 0.25 deg. X 0.25 deg. grids, therefore they conserve all the Level 2 (L2) spatial and temporal details for 24 hours of scientific data in one file. The second Modern-Era Retrospective analysis for Research and Applications (MERRA-2) is NASA's atmospheric reanalysis, using an upgraded version of Goddard Earth Observing System Model, version 5 (GEOS-5) data assimilation system. MERRA-2 includes aerosol data reanalysis and improved representations of stratospheric ozone, compared with its predecessor MERRA, in both instantaneous and time-averaged collections. It is found that simply comparing satellite Level-3 products might cause biases, due to lack of detailed temporal and original retrieval information. It is therefore preferable to inter-compare or implement satellite derived physical quantities directly with/to model assimilation with as high temporal and spatial resolutions as possible. This study will demonstrate utilization of OMI L2G daily aerosol and ozone products by comparing them with MERRA-2 hourly aerosol/ozone simulations, matched in both space and time aspects. Both OMI and MERRA-2 products are accessible online through NASA Goddard Earth Sciences Data Information Services Center (GES DISC, https://disc.gsfc.nasa.gov/)

Atomic-scale control of magnetic anisotropy via novel spin-orbit coupling effect in La2/3Sr1/3MnO3/SrIrO3 superlattices

Magnetic anisotropy (MA) is one of the most important material properties for modern spintronic devices. Conventional manipulation of the intrinsic MA, i.e. magnetocrystalline anisotropy (MCA), typically depends upon crystal symmetry. Extrinsic control over the MA is usually achieved by introducing shape anisotropy or exchange bias from another magnetically ordered material. Here we demonstrate a pathway to manipulate MA of 3d transition metal oxides (TMOs) by digitally inserting non-magnetic 5d TMOs with pronounced spin-orbit coupling (SOC). High quality superlattices comprised of ferromagnetic La2/3Sr1/3MnO3 (LSMO) and paramagnetic SrIrO3 (SIO) are synthesized with the precise control of thickness at atomic scale. Magnetic easy axis reorientation is observed by controlling the dimensionality of SIO, mediated through the emergence of a novel spin-orbit state within the nominally paramagnetic SIO.Comment: Proceedings of the National Academy of Sciences, May 201

Quasi-local energy and the choice of reference

A quasi-local energy for Einstein's general relativity is defined by the value of the preferred boundary term in the covariant Hamiltonian formalism. The boundary term depends upon a choice of reference and a time-like displacement vector field (which can be associated with an observer) on the boundary of the region. Here we analyze the spherical symmetric cases. For the obvious analytic choice of reference based on the metric components, we find that this technique gives the same quasi-local energy values using several standard coordinate systems and yet can give different values in some other coordinate systems. For the homogeneous-isotropic cosmologies, the energy can be non-positive, and one case which is actually flat space has a negative energy. As an alternative, we introduce a way to determine the choice of both the reference and displacement by extremizing the energy. This procedure gives the same value for the energy in different coordinate systems for the Schwarzschild space, and a non-negative value for the cosmological models, with zero energy for the dynamic cosmology which is actually Minkowski space. The timelike displacement vector comes out to be the dual mean curvature vector of the two-boundary.Comment: 21 pages; revised version to appear in CQ

Phosphorylation Mechanism of Phosphomevalonate Kinase: Implications for Rational Engineering of Isoprenoid Biosynthetic Pathway Enzymes

The mevalonate pathway is of important clinical, pharmaceutical, and biotechnological relevance. However, lack of the understanding of the phosphorylation mechanism of the kinases in this pathway has limited rationally engineering the kinases in industry. Here the phosphorylation reaction mechanism of a representative kinase in the mevalonate pathway, phosphomevalonate kinase, was studied by using molecular dynamics and hybrid QM/MM methods. We find that a conserved residue (Ser106) is reorientated to anchor ATP via a stable H-bond interaction. In addition, Ser213 located on the α-helix at the catalytic site is repositioned to further approach the substrate, facilitating the proton transfer during the phosphorylation. Furthermore, we elucidate that Lys101 functions to neutralize the negative charge developed at the β-, γ-bridging oxygen atom of ATP during phosphoryl transfer. We demonstrate that the dissociative catalytic reaction occurs via a direct phosphorylation pathway. This is the first study on the phosphorylation mechanism of a mevalonate pathway kinase. The elucidation of the catalytic mechanism not only sheds light on the common catalytic mechanism of the GHMP kinase superfamily but also provides the structural basis for engineering the mevalonate pathway kinases to further exploit their applications in the production of a wide range of fine chemicals such as biofuels or pharmaceuticals

Pharmacologically blocking p53-dependent apoptosis protects intestinal stem cells and mice from radiation.

Exposure to high levels of ionizing radiation (IR) leads to debilitating and dose-limiting gastrointestinal (GI) toxicity. Using three-dimensional mouse crypt culture, we demonstrated that p53 target PUMA mediates radiation-induced apoptosis via a cell-intrinsic mechanism, and identified the GSK-3 inhibitor CHIR99021 as a potent radioprotector. CHIR99021 treatment improved Lgr5+ cell survival and crypt regeneration after radiation in culture and mice. CHIR99021 treatment specifically blocked apoptosis and PUMA induction and K120 acetylation of p53 mediated by acetyl-transferase Tip60, while it had no effect on p53 stabilization, phosphorylation or p21 induction. CHIR99021 also protected human intestinal cultures from radiation by PUMA but not p21 suppression. These results demonstrate that p53 posttranslational modifications play a key role in the pathological and apoptotic response of the intestinal stem cells to radiation and can be targeted pharmacologically

Hypothalamic Proopiomelanocortin Neurons Are Glucose Responsive and Express KATP Channels

Hypothalamic proopiomelanocortin (POMC) neurons are critical for controlling homeostatic functions in the mammal. We used a transgenic mouse model in which the POMC neurons were labeled with enhanced green fluorescent protein to perform visualized, whole-cell patch recordings from prepubertal female hypothalamic slices. The mouse POMC-enhanced green fluorescent protein neurons expressed the same endogenous conductances (a transient outward K current and a hyperpolarization-activated, cation current) that have been described for guinea pig POMC neurons. In addition, the selective -opioid receptor agonist DAMGO induced an outward current (maximum of 12.8 1.2 pA), which reversed at K equilibrium potential (EK), in the majority (85%) of POMC neurons with an EC50 of 102 nM. This response was blocked by the opioid receptor antagonist naloxone with an inhibition constant of 3.1 nM. In addition, the -aminobutyric acidB receptor agonist baclofen (40 M) caused an outward current (21.6 4.0 pA) that reversed at EK in these same neurons. The ATP-sensitive potassium channel opener diazoxide also induced an outward K current (maximum of 18.7 2.2 pA) in the majority (92%) of POMC neurons with an EC50 of 61 M. The response to diazoxide was blocked by the sulfonylurea tolbutamide, indicating that the POMC neurons express both Kir6.2 and sulfonylurea receptor 1 channel subunits, which was verified using single cell RT-PCR. This pharmacological and molecular profile suggested that POMC neurons might be sensitive to metabolic inhibition, and indeed, we found that their firing rate varied with changes in glucose concentrations. Therefore, it appears that POMC neurons may function as an integrator of metabolic cues and synaptic input for controlling homeostasis in the mammal