Is tagging with visual implant elastomer a reliable technique for marking earthworms?

Visual implant elastomer (VIE) has recently been employed to investigate different aspects of earthworm ecology. However, a number of fundamental questions relating to the detection and positioning of the tag, its persistence and potential effects on earthworms remain unknown. Seven earthworm species belonging to three ecological groupings, with different pigmentation and burrowing behaviour, were tagged using different coloured VIE. External inspection after two days, one week and 1, 10 and 27 months were followed by preservation, dissection and internal inspection. Tags could be seen in living specimens to 27 months, and dissection revealed that in most cases they were lodged in the coelomic cavity, held in place by septa. However, over longer time periods (more than two years), the chlorogogenous tissue tended to bind to the tags and made external observation increasingly difficult. Migration of the VIE material towards the posterior of the earthworm and potential loss of the tag were only observed on rare occasions, and a recovery rate in excess of 98% was recorded. By introducing a reasonable amount of VIE into segments, just after the clitellum, this technique can become a valuable tool in earthworm ecology and life history studies, particularly in short-medium term laboratory and field experiments

Water intake, faecal output and intestinal motility in horses moved from pasture to a stabled management regime with controlled exercise

Reasons for performing study: A change in management from pasture to stabling is a risk factor for equine colic. Objectives: To investigate the effect of a management change from pasture with no controlled exercise to stabling with light exercise on aspects of gastrointestinal function related to large colon impaction. The hypothesis was that drinking water intake, faecal output, faecal water content and large intestinal motility would be altered by a transition from a pastured to a stabled regime. Study design: Within-subject management intervention trial involving changes in feeding and exercise using noninvasive techniques. Methods: Seven normal horses were evaluated in a within-subjects study design. Horses were monitored while at pasture 24 h/day, and for 14 days following a transition to a stabling regime with light controlled exercise. Drinking water intake, faecal output and faecal dry matter were measured. Motility of the caecum, sternal flexure and left colon (contractions/min) were measured twice daily by transcutaneous ultrasound. Mean values were pooled for the pastured regime and used as a reference for comparison with stabled data (Days 1–14 post stabling) for multilevel statistical analysis. Results: Drinking water intake was significantly increased (mean ± s.d. pasture 2.4 ± 1.8 vs. stabled 6.4 ± 0.6 l/100 kg bwt/day), total faecal output was significantly decreased (pasture 4.62 ± 1.69 vs. stabled 1.81 ± 0.5 kg/100 kg bwt/day) and faecal dry matter content was significantly increased (pasture 18.7 ± 2.28 vs. stabled 27.2 ± 1.93% DM/day) on all days post stabling compared with measurements taken at pasture (P<0.05). Motility was significantly decreased in all regions of the large colon collectively on Day 2 post stabling (-0.76 contractions/min), and in the left colon only on Day 4 (-0.62 contractions/min; P<0.05). Conclusions: There were significant changes in large intestinal motility patterns and parameters relating to gastrointestinal water balance during a transition from pasture to stabled management, particularly during the first 5 days

Visualising high-dimensional Pareto relationships in two-dimensional scatterplots

Copyright © 2013 Springer-Verlag Berlin Heidelberg. The final publication is availablevia the DOI in this recordBook title: Evolutionary Multi-Criterion Optimization7th International Conference on Evolutionary Multi-Criterion Optimization (EMO 2013), Sheffield, UK, March 19-22, 2013The codebase for this paper is available at https://github.com/fieldsend/emo_2013_vizIn this paper two novel methods for projecting high dimensional data into two dimensions for visualisation are introduced, which aim to limit the loss of dominance and Pareto shell relationships between solutions to multi-objective optimisation problems. It has already been shown that, in general, it is impossible to completely preserve the dominance relationship when mapping from a higher to a lower dimension – however, approaches that attempt this projection with minimal loss of dominance information are useful for a number of reasons. (1) They may represent the data to the user of a multi-objective optimisation problem in an intuitive fashion, (2) they may help provide insights into the relationships between solutions which are not immediately apparent through other visualisation methods, and (3) they may offer a useful visual medium for interactive optimisation. We are concerned here with examining (1) and (2), and developing relatively rapid methods to achieve visualisations, rather than generating an entirely new search/optimisation problem which has to be solved to achieve the visualisation– which may prove infeasible in an interactive environment for real time use. Results are presented on randomly generated data, and the search population of an optimiser as it progresses. Structural insights into the evolution of a set-based optimiser that can be derived from this visualisation are also discussed

Systematic review of factors influencing decisions to limit treatment in the emergency department

Background Emergency physicians are frequently faced with making decisions regarding how aggressive to be in caring for critically ill patients. We aimed to identify factors that influence decisions to limit treatment in the Emergency Department (ED) through a systematic search of the available literature. Design Prospectively registered systematic review of studies employing any methodology to investigate factors influencing decisions to limit treatment in the ED. Medline and EMBASE were searched from their inception until January 2019. Methodological quality was assessed using the Mixed Methods Appraisal Tool, but no studies were excluded based on quality. Findings were summarised by narrative analysis. Results 10 studies published between 1998 and 2016 were identified for inclusion in this review, including seven cross-sectional studies investigating factors associated with treatment-limiting decisions, two surveys of physicians making treatment-limiting decisions and one qualitative study of physicians making treatment-limiting decisions. There was significant heterogeneity in patient groups, outcome measures, methodology and quality. Only three studies received a methodology-specific rating of ‘high quality’. Important limitations of the literature include the use of small single-centre retrospective cohorts often lacking a comparison group, and survey studies with low response rates employing closed-response questionnaires. Factors influencing treatment-limiting decisions were categorised into ‘patient and disease factors’ (age, chronic disease, functional limitation, patient and family wishes, comorbidity, quality of life, acute presenting disorder type, severity and reversibility), ‘hospital factors’ (colleague opinion, resource availability) and ‘non-patient healthcare factors’ (moral, ethical, social and cost factors). Conclusions Several factors influence decisions to limit treatment in the ED. Many factors are objective and quantifiable, but some are subjective and open to individual interpretation. This review highlights the complexity of the subject and the need for more robust research in this field

Diversity and impact of rare variants in genes encoding the platelet G protein-coupled receptors

Platelet responses to activating agonists are influenced by common population variants within or near G protein-coupled receptor (GPCR) genes that affect receptor activity. However, the impact of rare GPCR gene variants is unknown. We describe the rare single nucleotide variants (SNVs) in the coding and splice regions of 18 GPCR genes in 7,595 exomes from the 1,000-genomes and Exome Sequencing Project databases and in 31 cases with inherited platelet function disorders (IPFDs). In the population databases, the GPCR gene target regions contained 740 SNVs (318 synonymous, 410 missense, 7 stop gain and 6 splice region) of which 70 % had global minor allele frequency (MAF) < 0.05 %. Functional annotation using six computational algorithms, experimental evidence and structural data identified 156/740 (21 %) SNVs as potentially damaging to GPCR function, most commonly in regions encoding the transmembrane and C-terminal intracellular receptor domains. In 31 index cases with IPFDs (Gi-pathway defect n=15; secretion defect n=11; thromboxane pathway defect n=3 and complex defect n=2) there were 256 SNVs in the target regions of 15 stimulatory platelet GPCRs (34 unique; 12 with MAF< 1 % and 22 with MAF≥ 1 %). These included rare variants predicting R122H, P258T and V207A substitutions in the P2Y12 receptor that were annotated as potentially damaging, but only partially explained the platelet function defects in each case. Our data highlight that potentially damaging variants in platelet GPCR genes have low individual frequencies, but are collectively abundant in the population. Potentially damaging variants are also present in pedigrees with IPFDs and may contribute to complex laboratory phenotypes

The Seiberg-Witten Map for a Time-dependent Background

In this paper the Seiberg-Witten map for a time-dependent background related to a null-brane orbifold is studied. The commutation relations of the coordinates are linear, i.e. it is an example of the Lie algebra type. The equivalence map between the Kontsevich star product for this background and the Weyl-Moyal star product for a background with constant noncommutativity parameter is also studied.Comment: latex, 13 pages, references added and some misprints correcte

Cutaneous manifestations of hospitalized coronavirus disease 2019 patients: a report of six cases with clinicopathologic features and viral RNA in situ hybridization

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/163863/1/jdv16741.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/163863/2/jdv16741_am.pd

Multi-objective optimisation for receiver operating characteristic analysis

Copyright © 2006 Springer-Verlag Berlin Heidelberg. The final publication is available at link.springer.comBook title: Multi-Objective Machine LearningSummary Receiver operating characteristic (ROC) analysis is now a standard tool for the comparison of binary classifiers and the selection operating parameters when the costs of misclassification are unknown. This chapter outlines the use of evolutionary multi-objective optimisation techniques for ROC analysis, in both its traditional binary classification setting, and in the novel multi-class ROC situation. Methods for comparing classifier performance in the multi-class case, based on an analogue of the Gini coefficient, are described, which leads to a natural method of selecting the classifier operating point. Illustrations are given concerning synthetic data and an application to Short Term Conflict Alert

Agricultura familiar, multifuncionalidade da agricultura e ruralidade: interfaces de uma realidade complexa.

Nas últimas décadas, assistimos à revitalização do debate em torno da agricultura familiar pela incorporação das discussões sobre as múltiplas funções da agricultura num rural que não pode mais ser visto como domínio exclusivo da atividade agrícola e dos agricultores. No contexto brasileiro, acreditamos que essa discussão deva ser permeada pela análise de como processos diferenciados de distribuição fundiária e desenvolvimento econômico e o tipo de agricultura resultante geram ruralidades específicas com características como, no caso da agricultura familiar, a manutenção de práticas sociais e de trabalho marcadas pelas relações de parentesco e vizinhança presentes numa sociedade de interconhecimento. Partindo desse princípio, este trabalho analisa a agricultura familiar de um bairro rural do município de Ouro Fino - MG, sua herança histórica e sua participação na criação de uma paisagem natural e humana que muito contribui para a atratividade da região