Inhibiting cyclin-dependent kinase 5 in the nucleus accumbens enhances the expression of amphetamine-induced locomotor conditioning

When psychostimulant drugs like amphetamine are administered repeatedly in the presence of a contextual stimulus complex, long-lasting associations form between the unconditioned effects of the drug and the contextual stimuli. Here we assessed the role played by the proline-directed serine/threonine kinase cyclin-dependent kinase 5 (Cdk5) in the nucleus accumbens (NAcc) on the expression of the conditioned locomotion normally observed when rats are returned to a context previously paired with amphetamine. Infusing the Cdk5 inhibitor roscovitine (40 nmol/0.5µl/side) into the NAcc 30-min before the test for conditioning significantly enhanced the conditioned locomotor response observed in rats previously administered amphetamine in the test environment. This effect was specific to the expression of a conditioned response as inhibiting Cdk5 produced no effect in control rats previously administered saline or previously administered amphetamine elsewhere. As inhibiting Cdk5 during exposure to amphetamine has been found to block the accrual of locomotor conditioning, the present results suggest distinct roles for NAcc Cdk5 in the induction and expression of excitatory conditioning by amphetamine

A neglected aspect of human-elephant conflict: fence damage by elephants in the Trans Mara, Kenya

Human–elephant conflict (HEC) is one of the most complex issues for elephant conservation today and is on the increase. Incidents involving elephants can have severe consequences for people who co-exist with them, creating negative attitudes. While there has been a great deal of research on crop raiding, other forms of conflict including fence damage are poorly documented, but could still give rise to significant costs for households. In this study, we investigated the frequency, severity and patterns of fence damage caused by elephants in communities of the Trans Mara District, Kenya in 2014–2015 and compared these to patterns of crop raiding. In total there were 792 incidents involving fence damage only, 517 incidents involving crop and fence damage, and 72 incidents involving only crop damage. While the majority of fence damage incidents occurred between 18:00 and 06:00, some damage continued until 09:00. Fence damage occurred in every month of the study period and peaked when the frequency of crop damage decreased, highlighting the year-round nature of HEC in this region. The persistent occurrence of HEC in the Trans Mara, on both a daily and an annual timescale, becomes much more apparent when incidents of fence damage are considered as well as crop raiding. Such constant conflict could have significant implications for effects on human wellbeing and consequently, on local attitudes towards elephants and conservation efforts. This research highlights the need for more attention to be paid to the patterns and perceptions of all types of HEC, not just crop raiding, across elephant ranges in order to plan effective mitigation strategies

A framework for the prospective analysis of super-diversity coming from high levels of immigration

Background Pressures to keep immigration rates at relatively high levels are likely to persist in most developed countries. At the same time, immigrant cohorts are becoming more and more diverse, leading host societies to become increasingly heterogeneous across multiple dimensions. For scholars who study demographic or socio-economic behaviours, the need to account for ethnocultural “super-diversity” brings new challenges and complications. Objective The main objective of this paper is to present a framework for the prospective analysis of super-diversity in several high immigration countries. Methods We developed microsimulation models that simultaneously project several population-dimensions for Canada, the United States and countries of the European Union, with the aim of studying the consequences of alternate future population and migration trends. Results The paper presents the projected progression of three indicators of diversity for Canada, the USA and the EU28: percentage of foreign-born population, percentage of the population using a non-official language at home and percentage of non-Christians under the reference scenario. Results from alternative scenarios show the potential impact of modifying the composition of migrant cohorts. The paper also examines the projected changes in the labour force for each region by education level and language. Finally, the paper proposes a new longitudinal indicator that counts the number of years lived as active and inactive over the life course for foreign- and native-born cohorts. Contribution The microsimulation models provide much more informative results than more traditional cohort-component or multi-state models to study the future effects of ethnocultural super-diversity on high immigration countries

Neuronal and psychological underpinnings of pathological gambling

Like in the case of drugs, gambling hijacks reward circuits in a brain which is not prepared to receive such intense stimulation. Dopamine is normally released in response to reward and uncertainty in order to allow animals to stay alive in their environment – where rewards are relatively unpredictable. In this case, behavior is regulated by environmental feedbacks, leading animals to persevere or to give up. In contrast, drugs provide a direct, intense pharmacological stimulation of the dopamine system that operates independently of environmental feedbacks, and hence causes “motivational runaways”. With respect to gambling, the confined environment experienced by gamblers favors the emergence of excitatory conditioned cues, so that positive feedbacks take over negative feedbacks. Although drugs and gambling may act differently, their abnormal activation of reward circuitry generates an underestimation of negative consequences and promotes the development of addictive/compulsive behavior. In Parkinson’s and Huntington’s disease, dopamine-related therapies may disrupt these feedbacks on dopamine signalling, potentially leading to various addictions, including pathological gambling. The goal of this Research Topic is to further our understanding of the neurobiological mechanisms underlying the development of pathological gambling. This eBook contains a cross-disciplinary collection of research and review articles, ranging in scope from animal behavioral models to human imaging studies

What causes the decrease in haematocrit during egg production?

1. Anaemia has been reported in wild animals, typically associated with traumatic events or ill health. However, female birds routinely become \u27anaemic\u27 during egg-laying; we sought to determine the causes of this reduction in haematocrit. 2. Haematocrit in female European Starlings (Sturnus vulgaris Linnaeus) decreased between pre-breeding and egg-laying in 3 out of 4 years (the decrease was marginally non-significant in the fourth year). This was independent of changes in ambient temperature altering the metabolic requirements for thermoregulation. 3. There was a positive relationship between haematocrit and plasma levels of the yolk precursor vitellogenin among egg-laying birds, supporting the hypothesis that the initial reduction in haematocrit is caused by increased blood volume associated with osmoregulatory adjustments to elevated levels of yolk precursors. 4. However, haematocrit did not always recover upon cessation of egg production, remaining low a.t clutch completion (2 of 4 years), incubation (1 of 2 years) and chick rearing (1 of 4 years), suggesting an additional cause of the prolonged reduction in haematocrit. 5. Given the magnitude and prolonged nature of the changes in haematocrit we report, and the interannual variation in haematocrit even during chick-rearing (47-54%), we suggest that \u27anaemia\u27 associated with egg production might have implications for aerobic performance during later stages of breeding

Oxygen Cost of Performing Selected Adult and Child Care Activities

International Journal of Exercise Science 6(1) : 11-19, 2013. Little is known about the oxygen cost of caring for infants and older adults. Many people perform these activities so it is useful to know the energy cost and if the activities are of sufficient intensity to contribute to meeting physical activity recommendations. The purpose of this study was to assess the oxygen cost of four care-related activities in the Compendium of Physical Activities. Nineteen participants (n = 10 women, n = 9 men; Age = 36.4 ± 13.6 y; % Fat = 34.1 ± 10.5; BMI = 28.1 ± 4.5 kg/m2) performed four activities: 1) pushing an infant in a stroller, 2) pushing an adult in a wheelchair, 3) carrying an infant, and 4) bathing and dressing an infant. The oxygen cost was assessed using a portable metabolic unit. Activities were performed in random order for 8 minutes. The oxygen cost and heart rates, respectively, for healthy adults during care related activities were 3.09 METs and 90 ± 8 beats per minute (bpm) for pushing an infant in a stroller, 3.69 METs and 97 ± 9 bpm for pushing an adult in a wheelchair, 2.37 METs and 85 ± 9 bpm for carrying an infant, and 2.00 METs and 87 ± 9 bpm for bathing and dressing an infant. Carrying an infant and bathing an infant are light-intensity physical activities and pushing a wheelchair or a stroller are moderate intensity activities. The latter activities are of sufficient intensity to meet health-related physical activity recommendations

What causes the decrease in haematocrit during egg production?

1. Anaemia has been reported in wild animals, typically associated with traumatic events or ill health. However, female birds routinely become \u27anaemic\u27 during egg-laying; we sought to determine the causes of this reduction in haematocrit. 2. Haematocrit in female European Starlings (Sturnus vulgaris Linnaeus) decreased between pre-breeding and egg-laying in 3 out of 4 years (the decrease was marginally non-significant in the fourth year). This was independent of changes in ambient temperature altering the metabolic requirements for thermoregulation. 3. There was a positive relationship between haematocrit and plasma levels of the yolk precursor vitellogenin among egg-laying birds, supporting the hypothesis that the initial reduction in haematocrit is caused by increased blood volume associated with osmoregulatory adjustments to elevated levels of yolk precursors. 4. However, haematocrit did not always recover upon cessation of egg production, remaining low a.t clutch completion (2 of 4 years), incubation (1 of 2 years) and chick rearing (1 of 4 years), suggesting an additional cause of the prolonged reduction in haematocrit. 5. Given the magnitude and prolonged nature of the changes in haematocrit we report, and the interannual variation in haematocrit even during chick-rearing (47-54%), we suggest that \u27anaemia\u27 associated with egg production might have implications for aerobic performance during later stages of breeding

Abnormal glycosylation in Joubert syndrome type 10.

BACKGROUND: The discovery of disease pathogenesis requires systematic agnostic screening of multiple homeostatic processes that may become deregulated. We illustrate this principle in the evaluation and diagnosis of a 5-year-old boy with Joubert syndrome type 10 (JBTS10). He carried the OFD1 mutation p.Gln886Lysfs*2 (NM_003611.2: c.2656del) and manifested features of Joubert syndrome. METHODS: We integrated exome sequencing, MALDI-TOF mass spectrometry analyses of plasma and cultured dermal fibroblasts glycomes, and full clinical evaluation of the proband. Analyses of cilia formation and lectin staining were performed by immunofluorescence. Measurement of cellular nucleotide sugar levels was performed with high-performance anion-exchange chromatography with pulsed amperometric detection. Statistical analyses utilized the Student\u27s and Fisher\u27s exact t tests. RESULTS: Glycome analyses of plasma and cultured dermal fibroblasts identified abnormal N- and O-linked glycosylation profiles. These findings replicated in two unrelated males with OFD1 mutations. Cultured fibroblasts from affected individuals had a defect in ciliogenesis. The proband\u27s fibroblasts also had an abnormally elevated nuclear sialylation signature and increased total cellular levels of CMP-sialic acid. Ciliogenesis and each glycosylation anomaly were rescued by expression of wild-type OFD1. CONCLUSIONS: The rescue of ciliogenesis and glycosylation upon reintroduction of WT OFD1 suggests that both contribute to the pathogenesis of JBTS10

An overview of commonalities in the mechanisms underlying gambling and substance use disorders

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Cell wall biochemical alterations during Agrobacterium -mediated expression of haemagglutinin-based influenza virus-like vaccine particles in tobacco

International audienceInfluenza virus‐like particles (VLPs) have been shown to induce a safe and potent immune response through both humoral and cellular responses. They represent promising novel influenza vaccines. Plant‐based biotechnology allows for the large‐scale production of VLPs of biopharmaceutical interest using different model organisms, including Nicotiana benthamiana plants. Through this platform, influenza VLPs bud from the plasma membrane and accumulate between the membrane and the plant cell wall. To design and optimize efficient production processes, a better understanding of the plant cell wall composition of infiltrated tobacco leaves is a major interest for the plant biotechnology industry. In this study, we have investigated the alteration of the biochemical composition of the cell walls of N. benthamiana leaves subjected to abiotic and biotic stresses induced by the Agrobacterium‐mediated transient transformation and the resulting high expression levels of influenza VLPs. Results show that abiotic stress due to vacuum infiltration without Agrobacterium did not induce any detectable modification of the leaf cell wall when compared to non infiltrated leaves. In contrast, various chemical changes of the leaf cell wall were observed post‐Agrobacterium infiltration. Indeed, Agrobacterium infection induced deposition of callose and lignin, modified the pectin methylesterification and increased both arabinosylation of RG‐I side chains and the expression of arabinogalactan proteins. Moreover, these modifications were slightly greater in plants expressing haemagglutinin‐based VLP than in plants infiltrated with the Agrobacterium strain containing only the p19 suppressor of silencing